ROBERT J contact HONDALSummaryAffiliation: University of Vermont Country: USA Publications
Research Grants
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Detail Information
Publications
Differing views of the role of selenium in thioredoxin reductaseRobert J Hondal
Department of Biochemistry, College of Medicine, University of Vermont, Burlington, 05405, USA
Amino Acids 41:73-89. 2011....
Incorporation of selenocysteine into proteins using peptide ligationRobert J Hondal
Department of Biochemistry, Vermont College of Medicine, The University of Vermont, Burlington, VT 05405, USA
Protein Pept Lett 12:757-64. 2005..This replacement can confer unique structural and catalytic properties to enzymes and proteins. The development of this technique also allows for naturally occurring selenoproteins to be produced semisynthetically...- Using chemical approaches to study selenoproteins-focus on thioredoxin reductasesRobert J Hondal
Department of Biochemistry, University of Vermont, College of Medicine, 89 Beaumont Ave, Given Building Room B413, Burlington, VT 05405, USA
Biochim Biophys Acta 1790:1501-12. 2009..The enzyme utilizes a macromolecular mechanism for protein substrates, a second mechanism for small molecule substrates and a third pathway for selenium-containing substrates such as selenocystine... - No selenium required: reactions catalyzed by mammalian thioredoxin reductase that are independent of a selenocysteine residueAdam P Lothrop
Department of Biochemistry, University of Vermont, 89 Beaumont Avenue, Given Building Room B413, Burlington, Vermont 05405, USA
Biochemistry 48:6213-23. 2009..7 in the full-length mammalian enzyme (83-142 nM), but surprisingly the truncated enzyme showed much stronger inhibition (25 nM). This contrasts with auranofin, where the absence of Sec more strongly perturbed inhibition... - Synthesis of peptide substrates for mammalian thioredoxin reductaseStevenson Flemer
Department of Biochemistry, Given Laboratory, Burlington, VT 05405, USA
J Pept Sci 14:637-47. 2008..The results show that the ring structure contributes a factor of 52 to the rate, but the presence of selenium in the peptide is more important to catalysis than the presence of the ring... - Semisynthesis of proteins containing selenocysteineRobert J Hondal
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
Methods Enzymol 347:70-83. 2002 - Semisynthesis and characterization of mammalian thioredoxin reductaseBrian Eckenroth
Department of Biochemistry, University of Vermont, 89 Beaumont Avenue, Given Laboratory, Room B413, Burlington, Vermont 05405, USA
Biochemistry 45:5158-70. 2006..This noncovalent peptide-enzyme complex has 350-500-fold lower activity than the semisynthetic enzyme produced by peptide ligation... - Structural and biochemical studies reveal differences in the catalytic mechanisms of mammalian and Drosophila melanogaster thioredoxin reductasesBrian E Eckenroth
Department of Biochemistry, University of Vermont College of Medicine, 89 Beaumont Avenue, Burlington, Vermont 05405, USA
Biochemistry 46:4694-705. 2007..This places the Sec residue farther away from the protonating histidine residue, but the lower pKa of Sec in comparison to that of Cys eliminates the need for Sec to be protonated... - Studies on deprotection of cysteine and selenocysteine side-chain protecting groupsKatharine M Harris
Department of Biochemistry, 89 Beaumont Ave, Given Laboratory, Room B413, Burlington, VT 05405, USA
J Pept Sci 13:81-93. 2007.... 
Selenium in thioredoxin reductase: a mechanistic perspectiveBrian M Lacey
Department of Biochemistry, College of Medicine, University of Vermont, 89 Beaumont Avenue, Given Building Room B413, Burlington, Vermont 05405, USA
Biochemistry 47:12810-21. 2008..Further study of the biochemical properties of the truncated Cys and Sec TR enzymes demonstrates that the chemical advantage conferred on the eukaryotic enzyme by a selenol is the ability to function at acidic pH...- Characterization of mitochondrial thioredoxin reductase from C. elegansBrian M Lacey
Department of Biochemistry, 89 Beaumont Ave, Given Laboratory, Room B413, Burlington, VT 05405, USA
Biochem Biophys Res Commun 346:629-36. 2006..The enzyme would reduce selenocysteine, but not hydrogen peroxide or insulin. The flanking glycine residues of the GCCG motif were mutated to serine. The mutants improved substrate binding, but decreased the catalytic rate...
Research Grants
- Selenium-Thioredoxin Reductase Studied by SemisynthesisROBERT HONDAL; Fiscal Year: 2009..The importance of the thioredoxin system in disease processes such as cancer, arthritis, and malaria gives impetus to the study of the enzyme mechanism for the development of potential therapeutic inhibitors. ..
- The Biological and Chemical Function of Selenium in EnzymesROBERT J contact HONDAL; Fiscal Year: 2010..Cancer cells must divide rapidly to cause pathogenesis and require increased expression of thioredoxin reductase to survive. ..