Research Topics
| LEXIE HOLLIDAYSummaryAffiliation: University of Florida Country: USA Publications
Research Grants
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Detail Information
Publications
Rational identification of enoxacin as a novel V-ATPase-directed osteoclast inhibitorEdgardo J Toro
Department of Orthodontics, University of Florida College of Dentistry, Gainesville, FL 32610, USA
Curr Protein Pept Sci 13:180-91. 2012....
Osteoclast polarization and orthodontic tooth movementL S Holliday
Department of Orthodontics, University of Florida College of Dentistry, Gainesville, Florida 32610, USA
Orthod Craniofac Res 12:105-12. 2009..One potential use of such agents is to block tooth movement in spatially discrete locations to provide orthodontic anchorage...
Interactions between vacuolar H+-ATPases and microfilaments in osteoclastsL Shannon Holliday
Department of Orthodontics, University of Florida College of Dentistry, Gainesville, Florida, USA
J Bioenerg Biomembr 37:419-23. 2005..Studies are ongoing to establish the physiological role of the microfilament-binding activity of subunit B in osteoclasts and in other cells...
Interstitial collagenase activity stimulates the formation of actin rings and ruffled membranes in mouse marrow osteoclastsL S Holliday
Department of Orthodontics, University of Florida College of Dentistry, Gainesville, FL 32610, USA
Calcif Tissue Int 72:206-14. 2003..These data support the hypothesis that cleaved collagen stimulates osteoclastic bone resorption by triggering cytoskeletal reorganization and transport of V-ATPase from cytoplasmic stores to ruffled membranes...
Vacuolar H+-ATPase binding to microfilaments: regulation in response to phosphatidylinositol 3-kinase activity and detailed characterization of the actin-binding site in subunit BShih-Hua Chen
Department of Orthodontics, University of Florida College of Dentistry, Gainesville, Florida 32610, USA
J Biol Chem 279:7988-98. 2004..This response was fully reversible. The actin binding activities of the B subunits of V-ATPase required 11-amino acid actin-binding motifs that are similar in sequence to the actin-binding site of mammalian profilin I...
Identification of enoxacin as an inhibitor of osteoclast formation and bone resorption by structure-based virtual screeningDavid A Ostrov
Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL 32610, USA
J Med Chem 52:5144-51. 2009..In summary, enoxacin is a novel small molecule inhibitor of osteoclast bone resorption that acts by an unique mechanism and is therefore an attractive lead molecule for the development of a new class of antiosteoclastic agents...
Effects of basic fibroblast growth factor on osteoclasts and osteoclast-like cellsJian Zuo
Department of Orthodontics, University of Florida College of Dentistry, USA
Biochem Biophys Res Commun 318:162-7. 2004..7 osteoclast-like cells. These results suggest that FGF-2 has both direct and indirect effects on osteoclast formation and bone resorption...
Biochemical and functional characterization of the actin-binding activity of the B subunit of yeast vacuolar H+-ATPaseJian Zuo
Department of Orthodontics, University of Florida College of Dentistry, Gainesville, FL 32610, USA
J Exp Biol 211:1102-8. 2008..These results suggest that actin-binding activity confers on the B subunit of yeast a function that is distinct from its role in the enzymatic activity of the proton pump...
The amino-terminal domain of the E subunit of vacuolar H(+)-ATPase (V-ATPase) interacts with the H subunit and is required for V-ATPase functionMing Lu
Department of Medicine University of Florida College of Medicine, Gainesville, Florida 32610, USA
J Biol Chem 277:38409-15. 2002..Our data demonstrate the physiological significance of the interaction between the E and H subunits of V-ATPase and extend previous studies on the arrangement of subunits on the peripheral stalk of V-ATPase...
Calcium hydroxide reduces lipopolysaccharide-stimulated osteoclast formationJin Jiang
College of Dentistry, Department of Endodontics, University of Florida, Gainsville 32610 0436, USA
Oral Surg Oral Med Oral Pathol Oral Radiol Endod 95:348-54. 2003..The purpose of this study was to determine the direct effects of lipopolysaccharide (LPS) on osteoclastogenesis and to assess the ability of calcium hydroxide-Ca(OH)(2)-to inhibit the osteoclast formation stimulated by LPS...
The synergistic effect of peptidoglycan and lipopolysaccaride on osteoclast formationJin Jiang
Department of Endodontics, College of Dentistry, University of Florida, Gainesville 32610 0436, USA
Oral Surg Oral Med Oral Pathol Oral Radiol Endod 96:738-43. 2003..The purpose of this study was to investigate the mechanisms leading to periapical tissue destruction by the Gram-positive bacterial cell component, peptidoglycan (PGN) and Gram-negative bacterial cell component lipopolysaccaride (LPS)...
Actin binding activity of subunit B of vacuolar H+-ATPase is involved in its targeting to ruffled membranes of osteoclastsJian Zuo
Department of Orthodontics, University of Florida College of Dentistry, Gainesville, 32610, USA
J Bone Miner Res 21:714-21. 2006..The actin binding activity of B1 is involved in proper ruffled membrane targeting...
Actin-related protein 2/3 complex is required for actin ring formationI Rita Hurst
Department of Orthodontics, University of Florida College of Dentistry, Gainesville, Florida 32610-0444, USA
J Bone Miner Res 19:499-506. 2004..In addition, the results show the use of siRNAs for the study of RAW 264.7 osteoclast-like cells...
Regulation of vacuolar H(+)-ATPase in microglia by RANKLEric M Serrano
Department of Orthodontics, University of Florida College of Dentistry, Gainesville, FL 32610, USA
Biochem Biophys Res Commun 389:193-7. 2009..In summary, microglia express the a3-subunit of V-ATPase. The expression of a3 and the interaction between V-ATPases and microfilaments was modulated by RANKL. These data suggest a novel molecular pathway for regulating microglia...
Formation of GW/P bodies as marker for microRNA-mediated regulation of innate immune signaling in THP-1 cellsKaleb M Pauley
Department of Oral Biology, University of Florida, Gainesville, FL 32610 0424, USA
Immunol Cell Biol 88:205-12. 2010..These data show that the increase in size and number of GWB may serve as a biomarker for miRNA-mediated gene regulation, and miR-146a has a significant role in the regulation of LPS-induced cytokine production in THP-1 cells...
The 1.4-MDa apoptosome is a critical intermediate in apoptosome maturationElaine Beem
Division of Nephrology, Hypertension and Transplantation, Department of Medicine, University of Florida, PO Box 100224, Gainesville, FL 32610, USA
Am J Physiol Cell Physiol 287:C664-72. 2004..Consistent with these results, in vivo data suggest that blocking acidification during the induction of apoptosis inhibits activation of procaspase-3. On the basis of these results, we propose a model of apoptosome maturation...
Research Grants
- Osteoclast activation in uremic bone diseaseLEXIE HOLLIDAY; Fiscal Year: 2005....
