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Genomes and Genes | M HochstrasserSummaryAffiliation: University of Chicago Country: USA Publications
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Publications
The Saccharomyces cerevisiae ubiquitin-proteasome systemM Hochstrasser
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637, USA
Philos Trans R Soc Lond B Biol Sci 354:1513-22. 1999..Finally, it has become clear that protein (poly) ubiquitination is highly dynamic in vivo, and our studies of yeast de-ubiquitinating enzymes illustrate how such enzymes can facilitate the proteolysis of diverse substrates...
Ubiquitin-dependent protein degradationM Hochstrasser
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA
Annu Rev Genet 30:405-39. 1996..This diversity underlies both the high substrate specificity of the ubiquitin system and the variety of regulatory mechanisms that it serves...- The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 proteinS J Li
Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA
Mol Cell Biol 20:2367-77. 2000..Our data suggest the existence of a feedback mechanism that limits Smt3-protein ligation when Smt3 deconjugation by both Ulp1 and Ulp2 is compromised, allowing a partial recovery of cell function... - A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matalpha2 repressor degradationR Swanson
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
Genes Dev 15:2660-74. 2001..The likely human ortholog of DOA10 is in the cri-du-chat syndrome critical region on chromosome 5p, suggesting that defective ubiquitin ligation might contribute to this common genetic disorder... - The Doa4 deubiquitinating enzyme is required for ubiquitin homeostasis in yeastS Swaminathan
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
Mol Biol Cell 10:2583-94. 1999..We propose that Doa4 helps recycle ubiquitin from both proteasome-bound ubiquitinated intermediates and membrane proteins destined for destruction in the vacuole... - Eukaryotic 20S proteasome catalytic subunit propeptides prevent active site inactivation by N-terminal acetylation and promote particle assemblyC S Arendt
University of Chicago, Department of Biochemistry and Molecular Biology, 920 East 58th Street, Chicago, IL 60637, USA
EMBO J 18:3575-85. 1999..In addition to delineating a novel function for proteasome propeptides, these data provide the first biochemical evidence for the postulated participation of the alpha-amino group in the proteasome catalytic mechanism... - Interaction of the Doa4 deubiquitinating enzyme with the yeast 26S proteasomeF R Papa
Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA
Mol Biol Cell 10:741-56. 1999..Together, these data support a model in which Doa4 promotes proteolysis through removal of ubiquitin from proteolytic intermediates on the proteasome before or after initiation of substrate breakdown... - A new protease required for cell-cycle progression in yeastS J Li
University of Chicago, Department of Biochemistry and Molecular Biology, Illinois 60637, USA
Nature 398:246-51. 1999..The pattern of Smt3-coupled proteins in yeast changes markedly throughout the cell cycle, and specific conjugates accumulate in ulp1 mutants. Ulp1 has several functions, including an essential role in the G2/M phase of the cell cycle... - Degradation signal masking by heterodimerization of MATalpha2 and MATa1 blocks their mutual destruction by the ubiquitin-proteasome pathwayP R Johnson
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA
Cell 94:217-27. 1998.... - Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressorP Chen
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637
Cell 74:357-69. 1993..These data reveal an unexpected overlap in substrate specificity among diverse UBC enzymes and suggest a combinatorial mechanism of substrate selection in which UBC enzymes partition into multiple ubiquitination complexes... - Autocatalytic subunit processing couples active site formation in the 20S proteasome to completion of assemblyP Chen
Department of Biochemistry and Molecular Biology, The University of Chicago, Illinois 60637, USA
Cell 86:961-72. 1996.... - Identification of the yeast 20S proteasome catalytic centers and subunit interactions required for active-site formationC S Arendt
Department of Biochemistry and Molecular Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
Proc Natl Acad Sci U S A 94:7156-61. 1997.... - The yeast DOA4 gene encodes a deubiquitinating enzyme related to a product of the human tre-2 oncogeneF R Papa
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637
Nature 366:313-9. 1993..The human tre-2 oncogene encodes a deubiquitinating enzyme similar to Doa4, indicating a role for the ubiquitin system in mammalian growth control... - Biogenesis, structure and function of the yeast 20S proteasomeP Chen
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637, USA
EMBO J 14:2620-30. 1995.... - Analysis of the deubiquitinating enzymes of the yeast Saccharomyces cerevisiaeA Y Amerik
Yale University, Department of Molecular Biophysics and Biochemistry, New Haven, CT 06520, USA
Biol Chem 381:981-92. 2000..The data suggest that the DOA4 locus underwent a duplication after the divergence of K. lactis and S. cerevisiae. This information will facilitate fine-structure analysis of the Doa4 protein to help delineate its key functional elements... - The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathwaysA Y Amerik
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
Mol Biol Cell 11:3365-80. 2000.... - The yeast SEN3 gene encodes a regulatory subunit of the 26S proteasome complex required for ubiquitin-dependent protein degradation in vivoD J Demarini
Laboratory of Genetics and Molecular Biology, University of Wisconsin, Madison 53706, USA
Mol Cell Biol 15:6311-21. 1995.... - Molecular organization of the 20S proteasome gene family from Arabidopsis thalianaH Fu
Cellular and Molecular Biology Program and the Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706, USA
Genetics 149:677-92. 1998.... - Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particleD Finley
Trends Biochem Sci 23:244-5. 1998 - DUB-1, a deubiquitinating enzyme with growth-suppressing activityY Zhu
Division of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 93:3275-9. 1996..Our results suggest that DUB-1 regulates cellular growth by modulating either the ubiquitin-dependent proteolysis or the ubiquitination state of an unknown growth regulatory factor(s)... - An evolutionarily conserved gene on human chromosome 5q33-q34, UBH1, encodes a novel deubiquitinating enzymeT E Hansen-Hagge
Department of Pediatrics II, University of Ulm, Germany
Genomics 49:411-8. 1998..This new deubiquitinating enzyme has been named UBH1, for ubiquitin hydrolyzing enzyme 1... - In vivo degradation of a transcriptional regulator: the yeast alpha 2 repressorM Hochstrasser
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
Cell 61:697-708. 1990..This subunit-specific degradation makes possible a novel type of posttranslational remodeling in which a heteromeric protein could be functionally modified by selective, degradation-mediated replacement of its subunits...