PATRICIA HINKLE

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. ncbi Use of chimeric melanocortin-2 and -4 receptors to identify regions responsible for ligand specificity and dependence on melanocortin 2 receptor accessory protein
    Patricia M Hinkle
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, United States
    Eur J Pharmacol 660:94-102. 2011
  2. ncbi Role of TRH receptors as possible mediators of analeptic actions of TRH-like peptides
    Patricia M Hinkle
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, Box 711, 601 Elmwood Ave, Rochester, NY 14642, USA
    Brain Res 935:59-64. 2002
  3. ncbi Structure and function of the melanocortin2 receptor accessory protein (MRAP)
    Patricia M Hinkle
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, United States
    Mol Cell Endocrinol 300:25-31. 2009
  4. ncbi Endoplasmic reticulum calcium storage and release in cells expressing misfolded growth hormone
    Thomas K Graves
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Box 711, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Growth Horm IGF Res 13:36-43. 2003
  5. ncbi Regions of melanocortin 2 (MC2) receptor accessory protein necessary for dual topology and MC2 receptor trafficking and signaling
    Julien A Sebag
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 284:610-8. 2009
  6. ncbi Opposite effects of the melanocortin-2 (MC2) receptor accessory protein MRAP on MC2 and MC5 receptor dimerization and trafficking
    Julien A Sebag
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 284:22641-8. 2009
  7. ncbi Regulated dimerization of the thyrotropin-releasing hormone receptor affects receptor trafficking but not signaling
    Gyun Jee Song
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    Mol Endocrinol 19:2859-70. 2005
  8. ncbi Arrestin binds to different phosphorylated regions of the thyrotropin-releasing hormone receptor with distinct functional consequences
    Brian W Jones
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Mol Pharmacol 74:195-202. 2008
  9. ncbi Subcellular trafficking of the TRH receptor: effect of phosphorylation
    Brian W Jones
    Department of Pharmacology, University of Rochester Medical Center, Box 711, Rochester, New York 14642, USA
    Mol Endocrinol 23:1466-78. 2009
  10. ncbi Role of helix 8 of the thyrotropin-releasing hormone receptor in phosphorylation by G protein-coupled receptor kinase
    Austin U Gehret
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Mol Pharmacol 77:288-97. 2010

Research Grants

  1. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    PATRICIA HINKLE; Fiscal Year: 2000
  2. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    PATRICIA HINKLE; Fiscal Year: 2005
  3. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    PATRICIA HINKLE; Fiscal Year: 2007
  4. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    PATRICIA HINKLE; Fiscal Year: 1993
  5. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    Patricia M Hinkle; Fiscal Year: 2010

Collaborators

  • Carrie Haskell-Luevano
  • A E Pekary
  • Julie A Kelly
  • Brian W Jones
  • Julien A Sebag
  • Laurie B Cook
  • Gyun Jee Song
  • Austin U Gehret
  • Nicola Hogan
  • Thomas K Graves
  • Emileigh K Greuber
  • Chang Cheng Zhu
  • Kathy M O'Boyle
  • Gaia A Scalabrino
  • Chang-Cheng Zhu
  • Phuong N Tran
  • Carvell H Williams
  • Karl Bauer
  • Geoffrey W Bennett
  • Gillian R Slator
  • Daniel J Gregg
  • Sylvia M Draper
  • Keith F Tipton
  • Christopher M Fitchett

Detail Information

Publications23

  1. ncbi Use of chimeric melanocortin-2 and -4 receptors to identify regions responsible for ligand specificity and dependence on melanocortin 2 receptor accessory protein
    Patricia M Hinkle
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, United States
    Eur J Pharmacol 660:94-102. 2011
    ....
  2. ncbi Role of TRH receptors as possible mediators of analeptic actions of TRH-like peptides
    Patricia M Hinkle
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, Box 711, 601 Elmwood Ave, Rochester, NY 14642, USA
    Brain Res 935:59-64. 2002
    ..These results indicate that TRHR1 and TRHR2 do not mediate the behavioral effects of TRH-like peptides...
  3. ncbi Structure and function of the melanocortin2 receptor accessory protein (MRAP)
    Patricia M Hinkle
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, United States
    Mol Cell Endocrinol 300:25-31. 2009
    ..These results identify two distinct actions of MRAP: to permit trafficking of the MC2 receptor, and to allow surface receptor binding and signaling...
  4. ncbi Endoplasmic reticulum calcium storage and release in cells expressing misfolded growth hormone
    Thomas K Graves
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Box 711, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Growth Horm IGF Res 13:36-43. 2003
    ..CONCLUSION: Ca(2+) regulation is preserved despite retention of misfolded GH in the ER...
  5. ncbi Regions of melanocortin 2 (MC2) receptor accessory protein necessary for dual topology and MC2 receptor trafficking and signaling
    Julien A Sebag
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 284:610-8. 2009
    ..MRAP2 with an LDYI insertion functions like MRAP. These results demonstrate that MRAP not only facilitates MC2 receptor trafficking but also allows properly localized receptor to bind ACTH and consequently signal...
  6. ncbi Opposite effects of the melanocortin-2 (MC2) receptor accessory protein MRAP on MC2 and MC5 receptor dimerization and trafficking
    Julien A Sebag
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 284:22641-8. 2009
    ..These results establish that MRAP forms stable complexes with two different melanocortin receptors, facilitating surface expression of MC2 receptor but disrupting dimerization and surface localization of MC5 receptor...
  7. ncbi Regulated dimerization of the thyrotropin-releasing hormone receptor affects receptor trafficking but not signaling
    Gyun Jee Song
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    Mol Endocrinol 19:2859-70. 2005
    ..The results show that controlled dimerization of the TRH receptor potentiates hormone-induced receptor trafficking...
  8. ncbi Arrestin binds to different phosphorylated regions of the thyrotropin-releasing hormone receptor with distinct functional consequences
    Brian W Jones
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Mol Pharmacol 74:195-202. 2008
    ..Arrestin binding at either site causes increased agonist affinity and acid/salt resistance, but only the proximal phosphosites evoke the necessary conformational changes in arrestin for receptor desensitization and internalization...
  9. ncbi Subcellular trafficking of the TRH receptor: effect of phosphorylation
    Brian W Jones
    Department of Pharmacology, University of Rochester Medical Center, Box 711, Rochester, New York 14642, USA
    Mol Endocrinol 23:1466-78. 2009
    ....
  10. ncbi Role of helix 8 of the thyrotropin-releasing hormone receptor in phosphorylation by G protein-coupled receptor kinase
    Austin U Gehret
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Mol Pharmacol 77:288-97. 2010
    ..The results indicate that positive residues in helix 8 of GPCRs are important for GRK-dependent phosphorylation...
  11. ncbi Importance of regions outside the cytoplasmic tail of G-protein-coupled receptors for phosphorylation and dephosphorylation
    Austin U Gehret
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, U S A
    Biochem J 428:235-45. 2010
    ..Thus the interactions of GPCRs with GRKs and phosphatases are determined not simply by the amino acid sequences of the substrates, but by regions outside the cytoplasmic tails...
  12. ncbi Regulation of G protein-coupled receptor signaling: specific dominant-negative effects of melanocortin 2 receptor accessory protein 2
    Julien A Sebag
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Sci Signal 3:ra28. 2010
    ..These results demonstrate that the balance of stimulatory and inhibitory accessory proteins can control the sensitivity of a GPCR to its natural agonist...
  13. ncbi Melanocortin-2 receptor accessory protein MRAP forms antiparallel homodimers
    Julien A Sebag
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 104:20244-9. 2007
    ..MRAP is the first eukaryotic membrane protein identified with an antiparallel homodimeric structure...
  14. ncbi Dimerization and phosphorylation of thyrotropin-releasing hormone receptors are modulated by agonist stimulation
    Chang Cheng Zhu
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 277:28228-37. 2002
    ..TRH also increased phosphorylation and dimerization of TRH receptors expressed in GHFT pre-lactotroph cells...
  15. ncbi Thyrotropin-releasing hormone receptor processing: role of ubiquitination and proteasomal degradation
    Laurie B Cook
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    Mol Endocrinol 17:1777-91. 2003
    ..These results show that ubiquitin modification targets misfolded receptors for degradation and suggest a possible role for ubiquitination in receptor trafficking...
  16. ncbi Ca(2+)-induced Ca(2+) release in the pancreatic beta-cell: direct evidence of endoplasmic reticulum Ca(2+) release
    Thomas K Graves
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    Endocrinology 144:3565-74. 2003
    ..Taken together, these results provide strong evidence that Ca(2+)-induced Ca(2+) release augments cytoplasmic Ca(2+) signals in pancreatic beta-cells...
  17. ncbi Agonist-dependent up-regulation of thyrotrophin-releasing hormone receptor protein
    Laurie B Cook
    Department of Pharmacology and Physiology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Biochem J 380:815-21. 2004
    ..These results show that TRH increases receptor concentration by a complex mechanism that requires signal transduction but not receptor endocytosis...
  18. ncbi Fate of internalized thyrotropin-releasing hormone receptors monitored with a timer fusion protein
    Laurie B Cook
    Department of Pharmacology and Physiology, Box 711, University of Rochester Medical Center, Rochester, New York 14642, USA
    Endocrinology 145:3095-100. 2004
    ..The results indicate that, after agonist-driven receptor internalization, the plasma membrane is replenished with younger receptors, arising either from an intracellular pool or preferential recycling of younger receptors...
  19. ncbi Beta-arrestin mediates desensitization and internalization but does not affect dephosphorylation of the thyrotropin-releasing hormone receptor
    Brian W Jones
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 280:38346-54. 2005
    ..The results show conclusively that receptor dephosphorylation can take place on the plasma membrane and that beta-arrestin binding is critical for desensitization and internalization...
  20. ncbi Phosphorylation of the endogenous thyrotropin-releasing hormone receptor in pituitary GH3 cells and pituitary tissue revealed by phosphosite-specific antibodies
    Brian W Jones
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 282:12893-906. 2007
    ....
  21. ncbi Dimerization of the thyrotropin-releasing hormone receptor potentiates hormone-dependent receptor phosphorylation
    Gyun Jee Song
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 104:18303-8. 2007
    ..These results suggest that the TRH receptor is phosphorylated preferentially when it is in dimers or when preexisting receptor dimers are driven into microaggregates. Increased receptor phosphorylation may amplify desensitization...
  22. ncbi Discovery of a dual action first-in-class peptide that mimics and enhances CNS-mediated actions of thyrotropin-releasing hormone
    Gaia A Scalabrino
    School of Biochemistry and Immunology and Trinity College Institute of Neuroscience, Trinity College Dublin, Dublin 2, Ireland
    Neuropharmacology 52:1472-81. 2007
    ..This discovery provides new opportunities for probing the role of TRH actions in the CNS and a basis for development of novel TRH-based neurotherapeutics...
  23. ncbi A novel TRH analog, Glp-Asn-Pro-D-Tyr-D-TrpNH2, binds to [3H][3-Me-His2]TRH-labelled sites in rat hippocampus and cortex but not pituitary or heterologous cells expressing TRHR1 or TRHR2
    Nicola Hogan
    UCD School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland
    Neurosci Lett 431:26-30. 2008
    ..Also, the results raise the possibility that Glp-Asn-Pro-D-Tyr-D-TrpNH(2) is not displacing [(3)H][3-Me-His(2)]TRH from a known TRH receptor in rat cortex, but rather a hitherto unidentified TRH receptor...

Research Grants26

  1. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    PATRICIA HINKLE; Fiscal Year: 2000
    ....
  2. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    PATRICIA HINKLE; Fiscal Year: 2005
    ....
  3. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    PATRICIA HINKLE; Fiscal Year: 2007
    ....
  4. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    PATRICIA HINKLE; Fiscal Year: 1993
    ..4. Once methods have been established for the biochemical identification TRH receptors and for monitoring TRH responses on single cells, these techniques will be applied to normal pituitary cells...
  5. MODULATION OF RECEPTOR NUMBER IN CULTURED CELLS
    Patricia M Hinkle; Fiscal Year: 2010
    ..abstract_text> ..