Donald Hilgemann

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi Biochemistry. Oily barbarians breach ion channel gates
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern, Dallas, TX 75235, USA
    Science 304:223-4. 2004
  2. pmc Dual control of cardiac Na+ Ca2+ exchange by PIP(2): electrophysiological analysis of direct and indirect mechanisms
    Alp Yaradanakul
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9040, USA
    J Physiol 582:991-1010. 2007
  3. pmc Massive Ca-induced membrane fusion and phospholipid changes triggered by reverse Na/Ca exchange in BHK fibroblasts
    Alp Yaradanakul
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 132:29-50. 2008
  4. pmc Steady-state function of the ubiquitous mammalian Na/H exchanger (NHE1) in relation to dimer coupling models with 2Na/2H stoichiometry
    Daniel Fuster
    Department of Physiology and Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Gen Physiol 132:465-80. 2008
  5. pmc Ion fluxes in giant excised cardiac membrane patches detected and quantified with ion-selective microelectrodes
    Tong Mook Kang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 9040, USA
    J Gen Physiol 121:325-47. 2003
  6. pmc Ca-dependent nonsecretory vesicle fusion in a secretory cell
    Tzu Ming Wang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 132:51-65. 2008
  7. pmc Massive calcium-activated endocytosis without involvement of classical endocytic proteins
    Vincenzo Lariccia
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 137:111-32. 2011
  8. pmc Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 137:155-72. 2011
  9. pmc Massive endocytosis driven by lipidic forces originating in the outer plasmalemmal monolayer: a new approach to membrane recycling and lipid domains
    Michael Fine
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 137:137-54. 2011
  10. doi Toward an understanding of the complete NCX1 lifetime in the cardiac sarcolemma
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA
    Adv Exp Med Biol 961:345-52. 2013

Research Grants

  1. Cardiac function and PIP2
    Donald Hilgemann; Fiscal Year: 2003
  2. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2007
  3. Cardiac function and PIP2
    Donald Hilgemann; Fiscal Year: 2007
  4. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2004
  5. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2005
  6. Cardiac function and PIP2
    Donald Hilgemann; Fiscal Year: 2006
  7. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2006
  8. Cardiac function and PIP2
    Donald Hilgemann; Fiscal Year: 2009
  9. FUNCTION AND REGULATION OF NA/CA EXCHANGERS
    Donald Hilgemann; Fiscal Year: 2003
  10. FUNCTION AND REGULATION OF NA/CA EXCHANGERS
    Donald Hilgemann; Fiscal Year: 1999

Collaborators

Detail Information

Publications28

  1. ncbi Biochemistry. Oily barbarians breach ion channel gates
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern, Dallas, TX 75235, USA
    Science 304:223-4. 2004
  2. pmc Dual control of cardiac Na+ Ca2+ exchange by PIP(2): electrophysiological analysis of direct and indirect mechanisms
    Alp Yaradanakul
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9040, USA
    J Physiol 582:991-1010. 2007
    ..We conclude that direct effects of PIP(2) to activate NCX1 can be strongly modulated by opposing mechanisms in intact cells that probably involve membrane cytoskeleton remodelling and membrane trafficking...
  3. pmc Massive Ca-induced membrane fusion and phospholipid changes triggered by reverse Na/Ca exchange in BHK fibroblasts
    Alp Yaradanakul
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 132:29-50. 2008
    ..In summary, our results provide no support for any regulatory or modulatory role of phospholipids in Ca-induced membrane fusion in fibroblasts...
  4. pmc Steady-state function of the ubiquitous mammalian Na/H exchanger (NHE1) in relation to dimer coupling models with 2Na/2H stoichiometry
    Daniel Fuster
    Department of Physiology and Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Gen Physiol 132:465-80. 2008
    ..We conclude that a large fraction of mammalian Na/H activity may occur with a 2Na/2H stoichiometry...
  5. pmc Ion fluxes in giant excised cardiac membrane patches detected and quantified with ion-selective microelectrodes
    Tong Mook Kang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 9040, USA
    J Gen Physiol 121:325-47. 2003
    ..The capability to monitor ion fluxes, independent of membrane currents, should facilitate studies of both electrogenic and electroneutral ion-coupled transporters in giant patches...
  6. pmc Ca-dependent nonsecretory vesicle fusion in a secretory cell
    Tzu Ming Wang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 132:51-65. 2008
    ....
  7. pmc Massive calcium-activated endocytosis without involvement of classical endocytic proteins
    Vincenzo Lariccia
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 137:111-32. 2011
    ..Thus, exocytosis is not prerequisite for MEND. From these results and two companion studies, we suggest that Ca promotes the formation of membrane domains that spontaneously vesiculate to the cytoplasmic side...
  8. pmc Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 137:155-72. 2011
    ..Collectively, the results indicate that >50% of the outer monolayer is ordered and can be selectively internalized during MEND responses initiated by two very different cell perturbations...
  9. pmc Massive endocytosis driven by lipidic forces originating in the outer plasmalemmal monolayer: a new approach to membrane recycling and lipid domains
    Michael Fine
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Gen Physiol 137:137-54. 2011
    ..We suggest that lateral and transbilayer inhomogeneities of the plasma membrane provide potential energies that, when unbridled by triggers, can drive endocytosis by lipidic forces...
  10. doi Toward an understanding of the complete NCX1 lifetime in the cardiac sarcolemma
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA
    Adv Exp Med Biol 961:345-52. 2013
    ..Second, we have developed mice that express fusion proteins of NCX1 with the pHluorin green protein. Thus, we can determine the membrane disposition of NCX1, and changes thereof, on-line in intact cardiac muscle...
  11. ncbi Local PIP(2) signals: when, where, and how?
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, 75390 9040, USA
    Pflugers Arch 455:55-67. 2007
    ..That PIP(2) synthesis and hydrolysis might be locally dependent on protein-protein interactions, and direct lipid "hand-off" is suggested by multiple results...
  12. pmc On the physiological roles of PIP(2) at cardiac Na+ Ca2+ exchangers and K(ATP) channels: a long journey from membrane biophysics into cell biology
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9040, USA
    J Physiol 582:903-9. 2007
    ..Thus, a better understanding of the regulation of cardiac lipid kinases may be key to understanding when and how cardiac ion transporters and channels are internalized...
  13. ncbi New insights into the molecular and cellular workings of the cardiac Na+/Ca2+ exchanger
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9040, USA
    Am J Physiol Cell Physiol 287:C1167-72. 2004
    ..It is speculated that the NCX1 gating reactions not only regulate coupled 3Na+:1Ca2+ exchange but also control the exchanger's Na+ leak function that generates background Na+ influx and depolarizing current in cardiac myocytes...
  14. ncbi Molecular control of cardiac sodium homeostasis in health and disease
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9040, USA
    J Cardiovasc Electrophysiol 17:S47-S56. 2006
    ..We present recent results on the regulation of all three transporters that may be important for an understanding of cardiac function during ischemia/reperfusion episodes...
  15. pmc From a pump to a pore: how palytoxin opens the gates
    Donald W Hilgemann
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9040, USA
    Proc Natl Acad Sci U S A 100:386-8. 2003
  16. ncbi Regulation of Kv7 (KCNQ) K+ channel open probability by phosphatidylinositol 4,5-bisphosphate
    Yang Li
    Department of Physiology, University of Texas Health Science Center, San Antonio, Texas 78229, USA
    J Neurosci 25:9825-35. 2005
    ..Divergent apparent affinities of Kv7.2-7.4 channels for PIP2 may underlie their highly differential maximal Po observed in cell-attached patches...
  17. ncbi Unrestricted diffusion of exogenous and endogenous PIP(2 )in baby hamster kidney and Chinese hamster ovary cell plasmalemma
    Alp Yaradanakul
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9040, USA
    J Membr Biol 220:53-67. 2007
    ..The lower mobility and incorporation of phospholipid at the extracellular leaflet may reflect a more ordered and condensed extracellular monolayer, as expected from previous studies...
  18. ncbi Protein kinase C inhibits ROMK1 channel activity via a phosphatidylinositol 4,5-bisphosphate-dependent mechanism
    Wei Zhong Zeng
    Department of Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8856, USA
    J Biol Chem 278:16852-6. 2003
    ..These results suggest that reduction of membrane PIP(2) content contributes to the inhibition of ROMK1 channels by PKC. This mechanism may underscore the inhibition of K(+) secretion in CCD by hormones that activate PKC...
  19. pmc Lipid- and mechanosensitivities of sodium/hydrogen exchangers analyzed by electrical methods
    Daniel Fuster
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235, USA
    Proc Natl Acad Sci U S A 101:10482-7. 2004
    ..Expressed in AP-1 cells, however, NHE1 is insensitive to these agents but remains sensitive to volume changes. Thus, changes of hydrophobic mismatch can modulate NHE1 but do not underlie its volume sensitivity...
  20. pmc Angiotensin II regulates neuronal excitability via phosphatidylinositol 4,5-bisphosphate-dependent modulation of Kv7 (M-type) K+ channels
    Oleg Zaika
    Department of Physiology, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    J Physiol 575:49-67. 2006
    ..The results of this study establish how angioII modulates M channels, which in turn affects the integrative properties of SCG neurons...
  21. pmc Dual control of cardiac Na+ Ca2+ exchange by PIP(2): analysis of the surface membrane fraction by extracellular cysteine PEGylation
    Chengcheng Shen
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9040, USA
    J Physiol 582:1011-26. 2007
    ....
  22. ncbi Modulation of cardiac PIP2 by cardioactive hormones and other physiologically relevant interventions
    Cem Nasuhoglu
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    Am J Physiol Cell Physiol 283:C223-34. 2002
    ..Inhibitors of several volume-sensitive signaling mechanisms do not affect these responses, suggesting that PIP2 metabolism might be sensitive to membrane tension, per se...
  23. ncbi Nonradioactive analysis of phosphatidylinositides and other anionic phospholipids by anion-exchange high-performance liquid chromatography with suppressed conductivity detection
    Cem Nasuhoglu
    Department of Physiology, Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9040, USA
    Anal Biochem 301:243-54. 2002
    ..In summary, the HPLC methods described here can be used to probe multiple aspects of phosphatidylinositide (Ptide) metabolism without radiolabeling...
  24. ncbi Multiple transport modes of the cardiac Na+/Ca2+ exchanger
    Tong Mook Kang
    Department of Physiology, Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440 746, Korea
    Nature 427:544-8. 2004
    ..The two minor transport modes can potentially determine resting free Ca2+ and background inward current in heart...
  25. pmc Phospholipase C in living cells: activation, inhibition, Ca2+ requirement, and regulation of M current
    Lisa F Horowitz
    Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle 98195, USA
    J Gen Physiol 126:243-62. 2005
    ..In each test, the suppression of KCNQ current closely paralleled the expected fall of PIP2. The results are described by a kinetic model...

Research Grants22

  1. Cardiac function and PIP2
    Donald Hilgemann; Fiscal Year: 2003
    ..Finally, the hypothesis will be tested that PIP2 metabolism is inherently sensitive to membrane tension and curvature, as well as to myocyte stretch (i.e. the cardiac preload). ..
  2. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2007
    ..4) In parallel with the experimental program, we will develop improved mathematical models of cardiac ion homeostasis with an emphasis on these three Na transporters. ..
  3. Cardiac function and PIP2
    Donald Hilgemann; Fiscal Year: 2007
    ..Finally, the hypothesis will be tested that PIP2 metabolism is inherently sensitive to membrane tension and curvature, as well as to myocyte stretch (i.e. the cardiac preload). ..
  4. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2004
    ..4) In parallel with the experimental program, we will develop improved mathematical models of cardiac ion homeostasis with an emphasis on these three Na transporters. ..
  5. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2005
    ..4) In parallel with the experimental program, we will develop improved mathematical models of cardiac ion homeostasis with an emphasis on these three Na transporters. ..
  6. Cardiac function and PIP2
    Donald Hilgemann; Fiscal Year: 2006
    ..Finally, the hypothesis will be tested that PIP2 metabolism is inherently sensitive to membrane tension and curvature, as well as to myocyte stretch (i.e. the cardiac preload). ..
  7. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2006
    ..4) In parallel with the experimental program, we will develop improved mathematical models of cardiac ion homeostasis with an emphasis on these three Na transporters. ..
  8. Cardiac function and PIP2
    Donald Hilgemann; Fiscal Year: 2009
    ..Our overall goal is a better understanding of the `life-time' and endocytosis of NCX1. This work can be expected to have fundamental implications for cardiac pathologies and ultimately medicine. ..
  9. FUNCTION AND REGULATION OF NA/CA EXCHANGERS
    Donald Hilgemann; Fiscal Year: 2003
    ..In total, therefore, our proposal is of broad physiological significance and is highly relevant to pathological roles of Ca in cardiac ischemia. ..
  10. FUNCTION AND REGULATION OF NA/CA EXCHANGERS
    Donald Hilgemann; Fiscal Year: 1999
    ..In total, therefore, our proposal is of broad physiological significance and is highly relevant to pathological roles of Ca in cardiac ischemia. ..
  11. FUNCTION AND REGULATION OF NA/CA EXCHANGERS
    Donald Hilgemann; Fiscal Year: 2000
    ..In total, therefore, our proposal is of broad physiological significance and is highly relevant to pathological roles of Ca in cardiac ischemia. ..
  12. FUNCTION AND REGULATION OF NA/CA EXCHANGERS
    Donald Hilgemann; Fiscal Year: 2001
    ..In total, therefore, our proposal is of broad physiological significance and is highly relevant to pathological roles of Ca in cardiac ischemia. ..
  13. FUNCTION AND REGULATION OF NA/CA EXCHANGERS
    Donald Hilgemann; Fiscal Year: 2002
    ..In total, therefore, our proposal is of broad physiological significance and is highly relevant to pathological roles of Ca in cardiac ischemia. ..
  14. Cardiac function and PIP2
    Donald W Hilgemann; Fiscal Year: 2010
    ..Our overall goal is a better understanding of the `life-time'and endocytosis of NCX1. This work can be expected to have fundamental implications for cardiac pathologies and ultimately medicine. ..