Wolf Dietrich Heyer

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Processing of joint molecule intermediates by structure-selective endonucleases during homologous recombination in eukaryotes
    Erin K Schwartz
    Department of Microbiology, University of California Davis, Davis, CA 95616, USA
    Chromosoma 120:109-27. 2011
  2. pmc RAD54 controls access to the invading 3'-OH end after RAD51-mediated DNA strand invasion in homologous recombination in Saccharomyces cerevisiae
    Xuan Li
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 37:638-46. 2009
  3. pmc Reconstitution of recombination-associated DNA synthesis with human proteins
    Jessica L Sneeden
    Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, CA 95616 8665, USA
    Nucleic Acids Res 41:4913-25. 2013
  4. pmc Rad51 and Rad54 ATPase activities are both required to modulate Rad51-dsDNA filament dynamics
    Xuan Li
    Section of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 35:4124-40. 2007
  5. pmc Saccharomyces cerevisiae Mus81-Mms4 is a catalytic, DNA structure-selective endonuclease
    Kirk Tevebaugh Ehmsen
    Section of Microbiology and Section of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616 8665, USA
    Nucleic Acids Res 36:2182-95. 2008
  6. pmc A conserved sequence extending motif III of the motor domain in the Snf2-family DNA translocase Rad54 is critical for ATPase activity
    Xiao ping Zhang
    Department of Microbiology and Molecular Genetics, University of California Davis, Davis, California, United States of America
    PLoS ONE 8:e82184. 2013
  7. pmc Loop 2 in Saccharomyces cerevisiae Rad51 protein regulates filament formation and ATPase activity
    Xiao ping Zhang
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 37:158-71. 2009
  8. pmc A junction branch point adjacent to a DNA backbone nick directs substrate cleavage by Saccharomyces cerevisiae Mus81-Mms4
    Kirk Tevebaugh Ehmsen
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 37:2026-36. 2009
  9. pmc A truncated DNA-damage-signaling response is activated after DSB formation in the G1 phase of Saccharomyces cerevisiae
    Ryan Janke
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 38:2302-13. 2010
  10. pmc Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA
    Jie Liu
    Department of Microbiology, University of California Davis, Davis, CA, USA
    Nat Struct Mol Biol 17:1260-2. 2010

Research Grants

Collaborators

  • Xiao ping Zhang
  • Jie Liu
  • William D Wright
  • Scott W Morrical
  • Xuan Li
  • Vitold E Galkin
  • Xavier Veaute
  • Roland Kanaar
  • E Egelman
  • LORRAINE SYMINGTON
  • J A Nickoloff
  • Edwin Haghnazari
  • Vladimir I Bashkirov
  • Kirk Tevebaugh Ehmsen
  • Konstantin Kiianitsa
  • Erin K Schwartz
  • Jachen A Solinger
  • Kristina Herzberg
  • Michael Rolfsmeier
  • Sucheta Mukherjee
  • Jessica L Sneeden
  • Shannon J Ceballos
  • Ryan Janke
  • Jacqueline Schmuckli-Maurer
  • John R Yates
  • Naozumi Mimida
  • Jodie K Chin
  • Elena V Bashkirova
  • Francis Fabre
  • Lindsey Walsh
  • Perry M Kim
  • Inger Tappin
  • Sara M Grossi
  • Jerard Hurwitz
  • James E Evans
  • Kirk T Ehmsen
  • Henning Stahlberg
  • Greg Cantin
  • Jordan Mar
  • F K Khasanov
  • Hiroaki Ichikawa
  • Seiichi Toki
  • Hiroko Kitamoto
  • Marina Nakashima
  • Keishi Osakabe
  • Yuji Ito
  • Floyd E Romesberg
  • Scott Anderson
  • Alexey S Vlasenko
  • W Hayes McDonald
  • Ho Nguyen
  • Nicholas Billinton
  • Serge Gangloff
  • M Gordon Barker
  • Kimberly S Paffett
  • Allan Chan
  • Richard M Walmsley
  • G V Savchenko
  • E V Bashkirova
  • V G Korolev
  • V I Bashkirov

Detail Information

Publications46

  1. pmc Processing of joint molecule intermediates by structure-selective endonucleases during homologous recombination in eukaryotes
    Erin K Schwartz
    Department of Microbiology, University of California Davis, Davis, CA 95616, USA
    Chromosoma 120:109-27. 2011
    ....
  2. pmc RAD54 controls access to the invading 3'-OH end after RAD51-mediated DNA strand invasion in homologous recombination in Saccharomyces cerevisiae
    Xuan Li
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 37:638-46. 2009
    ....
  3. pmc Reconstitution of recombination-associated DNA synthesis with human proteins
    Jessica L Sneeden
    Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, CA 95616 8665, USA
    Nucleic Acids Res 41:4913-25. 2013
    ..Our results support a model whereby the high-fidelity replicative DNA polymerase δ performs recombination-associated DNA synthesis, with translesion synthesis polymerases providing a supportive role as in normal replication...
  4. pmc Rad51 and Rad54 ATPase activities are both required to modulate Rad51-dsDNA filament dynamics
    Xuan Li
    Section of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 35:4124-40. 2007
    ..These biochemical defects in the Rad51-K191R protein could lead to deficiencies in presynapsis (filament formation) and postsynapsis (filament disassembly) in vivo...
  5. pmc Saccharomyces cerevisiae Mus81-Mms4 is a catalytic, DNA structure-selective endonuclease
    Kirk Tevebaugh Ehmsen
    Section of Microbiology and Section of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616 8665, USA
    Nucleic Acids Res 36:2182-95. 2008
    ..The endogenous complex purified in either modification state is negligibly active on Holliday junctions. Hence, Holliday junction incision activity in vitro cannot be attributed to the Mus81-Mms4 heterodimer in isolation...
  6. pmc A conserved sequence extending motif III of the motor domain in the Snf2-family DNA translocase Rad54 is critical for ATPase activity
    Xiao ping Zhang
    Department of Microbiology and Molecular Genetics, University of California Davis, Davis, California, United States of America
    PLoS ONE 8:e82184. 2013
    ..Our study identified a novel sequence motif critical for Rad54 function and showed that even perfect matches to the PIP box consensus may not necessarily identify PCNA interaction sites. ..
  7. pmc Loop 2 in Saccharomyces cerevisiae Rad51 protein regulates filament formation and ATPase activity
    Xiao ping Zhang
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 37:158-71. 2009
    ..No significant changes in the interaction between Rad51-K342E and Rad54 could be identified. These findings suggest that loop 2 contributes to the primary DNA-binding site in Rad51, controlling filament formation and ATPase activity...
  8. pmc A junction branch point adjacent to a DNA backbone nick directs substrate cleavage by Saccharomyces cerevisiae Mus81-Mms4
    Kirk Tevebaugh Ehmsen
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 37:2026-36. 2009
    ..These findings underscore the significance of a nick adjacent to a branch point for Mus81-Mms4 incision...
  9. pmc A truncated DNA-damage-signaling response is activated after DSB formation in the G1 phase of Saccharomyces cerevisiae
    Ryan Janke
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 38:2302-13. 2010
    ..These data suggest the existence of a truncated signaling pathway in response to a single DSB in G1-arrested cells that activates Mec1 without eliciting a full DDR involving the entire signaling pathway including the effector kinases...
  10. pmc Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA
    Jie Liu
    Department of Microbiology, University of California Davis, Davis, CA, USA
    Nat Struct Mol Biol 17:1260-2. 2010
    ..We report the purification of full-length human BRCA2 and show that it binds to ~6 RAD51 molecules and promotes RAD51 binding to ssDNA coated by replication protein A (RPA), in a manner that is stimulated by DSS1...
  11. pmc A new recombinational DNA repair gene from Schizosaccharomyces pombe with homology to Escherichia coli RecA
    F K Khasanov
    Institute of Gene Biology, Russian Academy of Sciences, Moscow 117 984, Russia
    Genetics 152:1557-72. 1999
    ..The structural and functional similarity suggests that rhp55(+) is a homolog of the S. cerevisiae RAD55 gene and we propose that the functional diversification of RecA-like genes in budding yeast is evolutionarily conserved...
  12. ncbi request reprint Holliday junctions in the eukaryotic nucleus: resolution in sight?
    Wolf Dietrich Heyer
    Division of Biological Sciences, Section of Microbiology, University of California, Davis, CA 95616 8665, USA
    Trends Biochem Sci 28:548-57. 2003
    ..Holliday junction resolution to non-crossover products can also be achieved topologically, for example, by the action of RecQ-like DNA helicases combined with topoisomerase III...
  13. ncbi request reprint Recombination: Holliday junction resolution and crossover formation
    Wolf Dietrich Heyer
    Division of Biological Sciences, Section of Microbiology and Center for Genetics and Development, University of California, Davis, California 95616 8665, USA
    Curr Biol 14:R56-8. 2004
    ..The intriguing substrate preference of this enzyme for nicked Holliday junctions opens the possibility that crossover formation may not always involve double Holliday junctions...
  14. pmc Rad54: the Swiss Army knife of homologous recombination?
    Wolf Dietrich Heyer
    Sections of Microbiology, University of California Davis, CA 95616 8665, USA
    Nucleic Acids Res 34:4115-25. 2006
    ..A wealth of genetic, cytological, biochemical and structural data suggests that Rad54 is a core factor of HR, possibly acting at multiple stages during HR in concert with the central homologous pairing protein Rad51...
  15. ncbi request reprint Recombination mechanisms; fortieth anniversary meeting of the Holliday model
    Wolf Dietrich Heyer
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, CA 95616 USA
    Mol Cell 16:1-9. 2004
    ..In those forty years the Holliday junction has gone from a hypothetical structure to models for its atomic structure and visualization of its dynamics at the single molecule level...
  16. ncbi request reprint Regulation of homologous recombination in eukaryotes
    Wolf Dietrich Heyer
    Department of Microbiology, University of California, Davis, Davis, California 95616 8665, USA
    Annu Rev Genet 44:113-39. 2010
    ..Here, we review the factors and mechanistic stages of recombination that are subject to regulation and suggest that recombination achieves flexibility and robustness by proceeding through metastable, reversible intermediates...
  17. ncbi request reprint Damage signaling: RecQ sends an SOS to you
    Wolf Dietrich Heyer
    Division of Biological Sciences, Section of Microbiology, Center for Genetics and Development, University of California at Davis, Davis, California 95616 8665, USA
    Curr Biol 14:R895-7. 2004
    ..Unwinding of stalled replication forks by RecQ family helicases in bacteria, and possibly in eukaryotes, may provide a means of damage signaling and recovering stalled replication forks...
  18. pmc Phosphorylation of Rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forks
    Kristina Herzberg
    Section of Microbiology, University of California, Davis, Davis, CA 95616 8665, USA
    Mol Cell Biol 26:8396-409. 2006
    ..These results suggest that Rad55-S2,8,14 phosphorylation activates recombinational repair, allowing for faster recovery after genotoxic stress...
  19. ncbi request reprint Rad54, a Swi2/Snf2-like recombinational repair protein, disassembles Rad51:dsDNA filaments
    Jachen A Solinger
    Division of Biological Sciences, Section of Microbiology, University of California Davis, Davis, CA 95616, USA
    Mol Cell 10:1175-88. 2002
    ..Rad51 protein is unable to release dsDNA upon ATP hydrolysis, leaving it stuck on the heteroduplex DNA product after DNA strand exchange. We suggest that Rad54 protein is involved in the turnover of Rad51-dsDNA filaments...
  20. ncbi request reprint Gly-103 in the N-terminal domain of Saccharomyces cerevisiae Rad51 protein is critical for DNA binding
    Xiao ping Zhang
    Section of Microbiology, University of California, Davis, California 95616 8665, USA
    J Biol Chem 280:26303-11. 2005
    ..Cell. Biol. 21, 966-976). Surprisingly, we found that the physical interaction of Rad51-G103E with Rad54 was not affected. Our data suggest that the two-hybrid interaction defect was an indirect consequence of the DNA binding defect...
  21. ncbi request reprint DNA damage-induced phosphorylation of Rad55 protein as a sentinel for DNA damage checkpoint activation in S. cerevisiae
    Vladimir I Bashkirov
    Section of Microbiology, University of California, Davis, USA
    Methods Enzymol 409:166-82. 2006
    ..We provide protocols to monitor the Rad55 phosphorylation status in vivo and assay Rad55-Rad57 phosphorylation in vitro using purified substrate with the Mec1 and Rad53 checkpoint kinases...
  22. ncbi request reprint The Hog1 MAP kinase pathway and the Mec1 DNA damage checkpoint pathway independently control the cellular responses to hydrogen peroxide
    Edwin Haghnazari
    Division of Biological Sciences, Section of Microbiology and Section of Molecular and Cellular Biology and Center for Genetics and Development, University of California, Davis, CA 95616 8665, USA
    DNA Repair (Amst) 3:769-76. 2004
    ....
  23. pmc Genome instability in rad54 mutants of Saccharomyces cerevisiae
    Jacqueline Schmuckli-Maurer
    Division of Biological Sciences, University of California, Davis, Davis, CA 95616 8665, USA
    Nucleic Acids Res 31:1013-23. 2003
    ....
  24. pmc Mus81-Mms4 functions as a single heterodimer to cleave nicked intermediates in recombinational DNA repair
    Erin K Schwartz
    Department of Microbiology, University of California, Davis, Davis, California, USA
    Mol Cell Biol 32:3065-80. 2012
    ..We infer that Mus81-dependent crossing over occurs in a noncanonical manner that does not involve the coordinated cleavage of classic Holliday junctions...
  25. pmc PCNA is required for initiation of recombination-associated DNA synthesis by DNA polymerase delta
    Xuan Li
    Department of Microbiology, University of California, Davis, 95616 8665, USA
    Mol Cell 36:704-13. 2009
    ..We conclude that PCNA has a specific role in the initiation of recombination-associated DNA synthesis and that DNA polymerase delta promotes recombination-associated DNA synthesis...
  26. pmc Direct kinase-to-kinase signaling mediated by the FHA phosphoprotein recognition domain of the Dun1 DNA damage checkpoint kinase
    Vladimir I Bashkirov
    Section of Microbiology and Center for Genetics and Development, Division of Biological Sciences, University of California, Davis, Davis, California 95616 8665, USA
    Mol Cell Biol 23:1441-52. 2003
    ..We show that the Dun1 FHA domain is critical for direct kinase-to-kinase signaling from Rad53 to Dun1 in the DNA damage checkpoint pathway...
  27. pmc The DNA damage checkpoint pathways exert multiple controls on the efficiency and outcome of the repair of a double-stranded DNA gap
    Edwin Haghnazari
    Division of Biological Sciences and Section of Microbiology, Section of Molecular and Cellular Biology and Center for Genetics and Development, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 32:4257-68. 2004
    ..The data suggest that the DNA damage checkpoints are important for the optimal functioning of recombinational DNA repair with multiple terminal targets to modulate the efficiency and outcome of homologous recombination...
  28. ncbi request reprint Presynaptic filament dynamics in homologous recombination and DNA repair
    Jie Liu
    Departments of Microbiology and of Molecular and Cellular Biology, University of California, Davis, CA, USA
    Crit Rev Biochem Mol Biol 46:240-70. 2011
    ..We proposed that the presynaptic filament has evolved to rely on multiple external factors for increased multilevel regulation of HR processes in genomes with greater structural and sequence complexity...
  29. pmc Rad51 paralogues Rad55-Rad57 balance the antirecombinase Srs2 in Rad51 filament formation
    Jie Liu
    Department of Microbiology, University of California, Davis, Davis, California 95616 8665, USA
    Nature 479:245-8. 2011
    ..These data provide a paradigm for the potential function of the human RAD51 paralogues, which are known to be involved in cancer predisposition and human disease...
  30. ncbi request reprint Rad54 protein exerts diverse modes of ATPase activity on duplex DNA partially and fully covered with Rad51 protein
    Konstantin Kiianitsa
    Division of Biological Sciences, Section of Microbiology, Center for Genetics and Development, University of California, Davis, California 95616 8665, USA
    J Biol Chem 277:46205-15. 2002
    ..Instead, saturated filaments with human Rad51 fail to support the yeast Rad54 ATPase. We suggest that the interaction of Rad54 with dsDNA-Rad51 complexes is of functional importance in homologous recombination...
  31. pmc Terminal association of Rad54 protein with the Rad51-dsDNA filament
    Konstantin Kiianitsa
    Sections of Microbiology and Molecular and Cellular Biology, Center for Genetics and Development, University of California Davis, Davis, CA 95616 8665
    Proc Natl Acad Sci U S A 103:9767-72. 2006
    ..Our data support a mechanism of processive dsDNA-Rad51 filament dissociation by the translocating Rad54 protein...
  32. pmc Homologous recombination in DNA repair and DNA damage tolerance
    Xuan Li
    Section of Microbiology University of California, Davis, Davis CA 95616 8665, USA
    Cell Res 18:99-113. 2008
    ..This review focuses on mechanistic aspects of HR relating to DSB and ICL repair as well as replication fork support...
  33. pmc Functions of the Snf2/Swi2 family Rad54 motor protein in homologous recombination
    Shannon J Ceballos
    Department of Microbiology, University of California, Davis, Davis, CA 95616 8665, USA
    Biochim Biophys Acta 1809:509-23. 2011
    ..We will discuss how these results relate to in vivo functions of Rad54 during homologous recombination in somatic cells and during meiosis. This article is part of a Special Issue entitled: Snf2/Swi2 ATPase structure and function...
  34. ncbi request reprint Some disassembly required: role of DNA translocases in the disruption of recombination intermediates and dead-end complexes
    Lorraine S Symington
    Department of Microbiology, Columbia University Medical Center, New York, New York 10032, USA
    Genes Dev 20:2479-86. 2006
  35. ncbi request reprint In vitro assays for DNA pairing and recombination-associated DNA synthesis
    Jie Liu
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Methods Mol Biol 745:363-83. 2011
    ..The quality of supercoiled plasmid DNA is critical in reconstituted HR reactions, and a protocol describing the preparation of this DNA substrate is included...
  36. ncbi request reprint Rad54 Functions as a Heteroduplex DNA Pump Modulated by Its DNA Substrates and Rad51 during D Loop Formation
    William Douglass Wright
    Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, CA 95616 8665, USA
    Mol Cell 53:420-32. 2014
    ..We present a unified model wherein Rad54 acts as an hDNA pump that drives D loop formation while simultaneously removing Rad51 from hDNA, consolidating both ATP-dependent activities of Rad54 into a single mechanistic step. ..
  37. ncbi request reprint Assays for structure-selective DNA endonucleases
    William D Wright
    Department of Microbiology, University of California, Davis, CA, USA
    Methods Mol Biol 745:345-62. 2011
    ..Determination of these values for a variety of substrates provides meaningful comparisons that allow inferences to be made regarding in vivo DNA structure target(s)...
  38. ncbi request reprint NADH-coupled microplate photometric assay for kinetic studies of ATP-hydrolyzing enzymes with low and high specific activities
    Konstantin Kiianitsa
    Section of Microbiology, Division of Biological Sciences, University of California, Davis, CA 95616 8665, USA
    Anal Biochem 321:266-71. 2003
  39. ncbi request reprint Quality control of purified proteins involved in homologous recombination
    Xiao ping Zhang
    Department of Microbiology, University of California, 95616 8665, Davis, CA 95616 8665, USA
    Methods Mol Biol 745:329-43. 2011
    ..These activities include ATPases, indicating the potential presence of helicases or translocases, endo- and exonucleases, phosphatases, and type I or type II topoisomerases...
  40. ncbi request reprint The Mus81-Mms4 structure-selective endonuclease requires nicked DNA junctions to undergo conformational changes and bend its DNA substrates for cleavage
    Sucheta Mukherjee
    Department of Microbiology and Molecular Genetics, University of California, One Shields Ave, Davis, Davis CA 95616 8665, USA
    Nucleic Acids Res 42:6511-22. 2014
    ..These data suggest that mutually compatible conformational changes of Mus81-Mms4 and its substrates tailor its incision activity to nicked junction molecules. ..
  41. pmc Alternate pathways involving Sgs1/Top3, Mus81/ Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication
    Francis Fabre
    Unité mixte de recherche 217, Centre National de la Recherche Scientifique Commissariat à l Energie Atomique Département de Radiobiologie et Radiopathologie BP6, 92265 Fontenay aux Roses, France
    Proc Natl Acad Sci U S A 99:16887-92. 2002
    ..Our results suggest that Srs2 channels recombination intermediates back into the gap-filling route, whereas Sgs1Top3 and Mus81Mms4 are involved in recombination andor in replication to allow replication restart...
  42. ncbi request reprint A new deal for Holliday junctions
    Wolf Dietrich Heyer
    Nat Struct Mol Biol 11:117-9. 2004
  43. ncbi request reprint DNA-damage induction of RAD54 can be regulated independently of the RAD9- and DDC1-dependent checkpoints that regulate RNR2
    Lindsey Walsh
    Department of Biomolecular Sciences, UMIST, PO Box 88, Manchester, M60 1QD, UK
    Curr Genet 41:232-40. 2002
    ..MBP1 was shown to be required for transcription of RNR2, but was not needed for transcription of RAD54. These results indicate that RNR2 and RAD54 are regulated in different ways...
  44. pmc Spontaneous and double-strand break-induced recombination, and gene conversion tract lengths, are differentially affected by overexpression of wild-type or ATPase-defective yeast Rad54
    Perry M Kim
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Nucleic Acids Res 30:2727-35. 2002
    ..Interestingly, ATPase(-) Rad54 decreased overall HR levels and increased tract lengths. These tract length changes provide new in vivo evidence that Rad54 functions in the post-synaptic phase during recombinational repair of DSBs...
  45. pmc Esc4/Rtt107 and the control of recombination during replication
    Jodie K Chin
    Department of Chemistry, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    DNA Repair (Amst) 5:618-28. 2006
    ..Thus, we propose that Esc4 associates with ssDNA of stalled forks and acts as a scaffolding protein to recruit and/or modulate the function of other proteins required to reinitiate DNA synthesis...
  46. ncbi request reprint Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments
    Naozumi Mimida
    National Institute of Agrobiological Sciences, 2 1 2 Kannondai, Tsukuba, 305 8602 Japan
    Plant Cell Physiol 48:648-54. 2007
    ..The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination...

Research Grants22

  1. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2000
    ..This will help to understand the role of Rad54p as well as identify and characterize novel DNA repair proteins. ..
  2. Microbial Production Core Facility
    Wolf Dietrich Heyer; Fiscal Year: 2007
    ..The requested instrumentation will not only make their work significantly more efficient, but will also allow the analysis of proteins that previously could not be purified in sufficient quantities or concentrations. ..
  3. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2007
    ..The pathways leading to resolution of junction structures in recombination are still unclear. Biochemical and in vivo experiments will determine the substrate-specificity and function of the Mus81-Mms4 endonuclease in HR. ..
  4. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2006
    ..The pathways leading to resolution of junction structures in recombination are still unclear. Biochemical and in vivo experiments will determine the substrate-specificity and function of the Mus81-Mms4 endonuclease in HR. ..
  5. Modulation of DNA double-strand break repair by posttranslational modification
    Wolf Dietrich Heyer; Fiscal Year: 2007
    ..4) Identify mechanisms of DNA damage checkpoint kinase regulation and test a positive feedback model involving Mec1 kinase. ..
  6. Modulation of DNA double-strand break repair by posttranslational modification
    Wolf Dietrich Heyer; Fiscal Year: 2009
    ..4) Identify mechanisms of DNA damage checkpoint kinase regulation and test a positive feedback model involving Mec1 kinase. ..
  7. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2009
    ....
  8. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2010
    ....
  9. Modulation of DNA double-strand break repair by posttranslational modification
    Wolf Dietrich Heyer; Fiscal Year: 2006
    ..4) Identify mechanisms of DNA damage checkpoint kinase regulation and test a positive feedback model involving Mec1 kinase. ..
  10. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2006
    ..The pathways leading to resolution of junction structures in recombination are still unclear. Biochemical and in vivo experiments will determine the substrate-specificity and function of the Mus81-Mms4 endonuclease in HR. ..
  11. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2001
    ..This will help to understand the role of Rad54p as well as identify and characterize novel DNA repair proteins. ..
  12. Modulation of DNA double strand break repair
    Wolf Dietrich Heyer; Fiscal Year: 2001
    ..4) Understanding the role of the DNA damage checkpoints in sensing DNA damage for the recombinational DNA repair pathway. ..
  13. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2002
    ..This will help to understand the role of Rad54p as well as identify and characterize novel DNA repair proteins. ..
  14. Modulation of DNA double strand break repair
    Wolf Dietrich Heyer; Fiscal Year: 2002
    ..4) Understanding the role of the DNA damage checkpoints in sensing DNA damage for the recombinational DNA repair pathway. ..
  15. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2003
    ..This will help to understand the role of Rad54p as well as identify and characterize novel DNA repair proteins. ..
  16. Modulation of DNA double strand break repair
    Wolf Dietrich Heyer; Fiscal Year: 2003
    ..4) Understanding the role of the DNA damage checkpoints in sensing DNA damage for the recombinational DNA repair pathway. ..
  17. RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTES
    Wolf Dietrich Heyer; Fiscal Year: 2005
    ..The pathways leading to resolution of junction structures in recombination are still unclear. Biochemical and in vivo experiments will determine the substrate-specificity and function of the Mus81-Mms4 endonuclease in HR. ..
  18. Modulation of DNA double strand break repair
    Wolf Dietrich Heyer; Fiscal Year: 2004
    ..4) Understanding the role of the DNA damage checkpoints in sensing DNA damage for the recombinational DNA repair pathway. ..
  19. Modulation of DNA double-strand break repair
    Wolf Dietrich Heyer; Fiscal Year: 2005
    ..4) Identify mechanisms of DNA damage checkpoint kinase regulation and test a positive feedback model involving Mec1 kinase. ..
  20. Modulation of DNA double-strand break repair by posttranslational modification.
    Wolf Dietrich Heyer; Fiscal Year: 2010
    ....