Samuel T Hess

Summary

Affiliation: University of Maine
Country: USA

Publications

  1. doi request reprint Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples
    Manuel F Juette
    Institute for Molecular Biophysics, The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA
    Nat Methods 5:527-9. 2008
  2. doi request reprint Ultrahigh resolution imaging of biomolecules by fluorescence photoactivation localization microscopy
    Samuel T Hess
    Department of Physics and Astronomy, University of Maine, Orono, ME, USA
    Methods Mol Biol 544:483-522. 2009
  3. pmc Imaging biological structures with fluorescence photoactivation localization microscopy
    Travis J Gould
    Department of Physics and Astronomy and Institute for Molecular Biophysics, University of Maine, Orono, Maine 04469, USA
    Nat Protoc 4:291-308. 2009
  4. pmc Super resolution microscopy reveals that caveolin-1 is required for spatial organization of CRFB1 and subsequent antiviral signaling in zebrafish
    Kristin A Gabor
    Graduate School of Biomedical Sciences, University of Maine, Orono, Maine, United States of America
    PLoS ONE 8:e68759. 2013
  5. pmc Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories
    Samuel T Hess
    Department of Physics and Astronomy and Institute for Molecular Biophysics, University of Maine, Orono, ME 04469, USA
    Proc Natl Acad Sci U S A 104:17370-5. 2007
  6. pmc Ultra-high resolution imaging by fluorescence photoactivation localization microscopy
    Samuel T Hess
    Department of Physics and Astronomy, University of Maine, Orono, ME 04469, USA
    Biophys J 91:4258-72. 2006
  7. pmc Actin mediates the nanoscale membrane organization of the clustered membrane protein influenza hemagglutinin
    Manasa V Gudheti
    Department of Physics and Astronomy, University of Maine, Orono, ME, USA
    Biophys J 104:2182-92. 2013
  8. pmc Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells
    Mudalige S Gunewardene
    Department of Physics and Astronomy and Institute for Molecular Biophysics, University of Maine, Orono, Maine, USA
    Biophys J 101:1522-8. 2011
  9. pmc Nanoscale imaging of molecular positions and anisotropies
    Travis J Gould
    Department of Physics and Astronomy and Institute for Molecular Biophysics, 5709 Bennett Hall, University of Maine, Orono, Maine 04469, USA
    Nat Methods 5:1027-30. 2008
  10. doi request reprint Chapter 12: Nanoscale biological fluorescence imaging: breaking the diffraction barrier
    Travis J Gould
    Department of Physics and Astronomy and Institute for Molecular Biophysics, University of Maine, Orono, Maine 04469, USA
    Methods Cell Biol 89:329-58. 2008

Research Grants

Collaborators

  • Vladislav V Verkhusha
  • Jeffrey A Yoder
  • Siew Hong Lam
  • Zhiyuan Gong
  • J Zimmerberg
  • Travis J Gould
  • Ahmed A Heikal
  • Manasa V Gudheti
  • Kristin A Gabor
  • Mudalige S Gunewardene
  • Tobias Baumgart
  • Carol H Kim
  • Jennifer A Rochira
  • Manuel F Juette
  • Watt W Webb
  • Juyoung Shim
  • Dahan Kim
  • Matthew J Pietraszewski
  • Chad R Stevens
  • Nikki M Curthoys
  • Julie A Gosse
  • Nicholas N Matluk
  • Fedor V Subach
  • Aldona A Karaczyn
  • Tamara L Adams
  • Joseph M Verdi
  • Leif Oxburgh
  • Gregory P Penoncello
  • Brian T Bennett
  • Michael J Mlodzianoski
  • Bhupendra S Nagpure
  • Mark D Lessard
  • Joerg Bewersdorf
  • Prabuddha Sengupta
  • David A Holowka
  • Barbara A Baird
  • Adam T Hammond
  • Michael Mlodzianoski

Detail Information

Publications18

  1. doi request reprint Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples
    Manuel F Juette
    Institute for Molecular Biophysics, The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA
    Nat Methods 5:527-9. 2008
    ..This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity...
  2. doi request reprint Ultrahigh resolution imaging of biomolecules by fluorescence photoactivation localization microscopy
    Samuel T Hess
    Department of Physics and Astronomy, University of Maine, Orono, ME, USA
    Methods Mol Biol 544:483-522. 2009
    ..It is hoped that these details can be used to perform FPALM on a variety of biological samples, to significantly advance the understanding of biological systems...
  3. pmc Imaging biological structures with fluorescence photoactivation localization microscopy
    Travis J Gould
    Department of Physics and Astronomy and Institute for Molecular Biophysics, University of Maine, Orono, Maine 04469, USA
    Nat Protoc 4:291-308. 2009
    ..Once alignment of the setup has been completed, data acquisitions can be obtained in approximately 1-30 min and analyzed in approximately 0.5-4 h...
  4. pmc Super resolution microscopy reveals that caveolin-1 is required for spatial organization of CRFB1 and subsequent antiviral signaling in zebrafish
    Kristin A Gabor
    Graduate School of Biomedical Sciences, University of Maine, Orono, Maine, United States of America
    PLoS ONE 8:e68759. 2013
    ....
  5. pmc Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories
    Samuel T Hess
    Department of Physics and Astronomy and Institute for Molecular Biophysics, University of Maine, Orono, ME 04469, USA
    Proc Natl Acad Sci U S A 104:17370-5. 2007
    ..In live cells, the dynamics of HA molecules within clusters is observed and quantified to determine an effective diffusion coefficient. The results are interpreted in terms of several established models of biological membranes...
  6. pmc Ultra-high resolution imaging by fluorescence photoactivation localization microscopy
    Samuel T Hess
    Department of Physics and Astronomy, University of Maine, Orono, ME 04469, USA
    Biophys J 91:4258-72. 2006
    ..This new method suggests a means to address a significant number of biological questions that had previously been limited by microscope resolution...
  7. pmc Actin mediates the nanoscale membrane organization of the clustered membrane protein influenza hemagglutinin
    Manasa V Gudheti
    Department of Physics and Astronomy, University of Maine, Orono, ME, USA
    Biophys J 104:2182-92. 2013
    ..Thus, with the use of imaging, we demonstrate a dynamic relationship between glycoprotein membrane organization and the actin cytoskeleton at the nanoscale...
  8. pmc Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells
    Mudalige S Gunewardene
    Department of Physics and Astronomy and Institute for Molecular Biophysics, University of Maine, Orono, Maine, USA
    Biophys J 101:1522-8. 2011
    ....
  9. pmc Nanoscale imaging of molecular positions and anisotropies
    Travis J Gould
    Department of Physics and Astronomy and Institute for Molecular Biophysics, 5709 Bennett Hall, University of Maine, Orono, Maine 04469, USA
    Nat Methods 5:1027-30. 2008
    ..We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin...
  10. doi request reprint Chapter 12: Nanoscale biological fluorescence imaging: breaking the diffraction barrier
    Travis J Gould
    Department of Physics and Astronomy and Institute for Molecular Biophysics, University of Maine, Orono, Maine 04469, USA
    Methods Cell Biol 89:329-58. 2008
    ..A detailed description of the methods involved in FPALM imaging of biological samples is presented here, accompanied by comparison with existing methods from the literature...
  11. pmc Imaging and shape analysis of GUVs as model plasma membranes: effect of trans DOPC on membrane properties
    Manasa V Gudheti
    Department of Physics and Astronomy, University of Maine, Orono, ME 04469, USA
    Biophys J 93:2011-23. 2007
    ..These changes in membrane properties seen in the presence of trans lipids could significantly impact cell function...
  12. pmc A small peptide modeled after the NRAGE repeat domain inhibits XIAP-TAB1-TAK1 signaling for NF-κB activation and apoptosis in P19 cells
    Jennifer A Rochira
    University of Maine, Orono, Maine, United States of America
    PLoS ONE 6:e20659. 2011
    ....
  13. pmc Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles
    Tobias Baumgart
    School of Applied and Engineering Physics and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 104:3165-70. 2007
    ..Thus, GPMVs now provide an effective approach to characterize biological membrane heterogeneities...
  14. pmc Quantitative electron microscopy and fluorescence spectroscopy of the membrane distribution of influenza hemagglutinin
    Samuel T Hess
    Laboratory for Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 169:965-76. 2005
    ..This work rules out the tested hypothesis for HA over the accessible length scales, yet shows clearly how the spatial distribution of HA depends on lipid composition...
  15. ncbi request reprint Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension
    Tobias Baumgart
    Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA
    Nature 425:821-4. 2003
    ..By analysing our observations using available membrane theory, we are able to provide experimental estimates of boundary tension between fluid bilayer domains...
  16. pmc Quantitative analysis of the fluorescence properties of intrinsically fluorescent proteins in living cells
    Samuel T Hess
    School of Applied and Engineering Physics, Nanobiotechnology Center, Cornell University, Ithaca, New York 14853, USA
    Biophys J 85:2566-80. 2003
    ..We also discuss the relevance of LynB-EGFP anisotropy for specialized domains studies in plasma membranes...
  17. pmc Focal volume optics and experimental artifacts in confocal fluorescence correlation spectroscopy
    Samuel T Hess
    Department of Physics, Cornell University, Ithaca, New York 14853, USA
    Biophys J 83:2300-17. 2002
    ..5 optical units) greatly reduces both the count rate per molecule and the signal-to-noise ratio. Thus, there is a tradeoff between optimizing signal-to-noise and reducing experimental artifacts in one-photon FCS...
  18. ncbi request reprint Biological and chemical applications of fluorescence correlation spectroscopy: a review
    Samuel T Hess
    Department of Physics and School of Applied and Engineering Physics, Clark Hall, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 41:697-705. 2002

Research Grants2

  1. The Role of Rafts in Virus-Induced Membrane Remodeling
    SAMUEL HESS; Fiscal Year: 2007
    ..Results will be used to attempt to better understand how influenza virus is able to congregate its own proteins for budding and entry (fusion). ..