Amy E Herr

Summary

Affiliation: University of California
Country: USA

Publications

  1. doi A lateral electrophoretic flow diagnostic assay
    Robert Lin
    Department of Bioengineering, UC Berkeley, Berkeley, CA 94720 USA
    Lab Chip 15:1488-96. 2015
  2. pmc Disruptive by design: a perspective on engineering in analytical chemistry
    Amy E Herr
    The UC Berkeley UCSF Graduate Program in Bioengineering, Berkeley, California, United States
    Anal Chem 85:7622-8. 2013
  3. doi Photopatterned free-standing polyacrylamide gels for microfluidic protein electrophoresis
    Todd A Duncombe
    University of California, Berkeley University of California, San Francisco Graduate Program in Bioengineering, 342 Stanley Hall, Berkeley, California 94720, USA
    Lab Chip 13:2115-23. 2013
  4. doi Protein post-translational modification analyses using on-chip immunoprobed isoelectric focusing
    Samuel Q Tia
    The UC Berkeley UCSF Graduate Program in Bioengineering, University of California, Berkeley, California 94720, United States
    Anal Chem 85:2882-90. 2013
  5. pmc Microchamber integration unifies distinct separation modes for two-dimensional electrophoresis
    Augusto M Tentori
    The UC Berkeley UCSF Graduate Program in Bioengineering, Berkeley, California 94720, United States
    Anal Chem 85:4538-45. 2013
  6. doi Microfluidic barcode assay for antibody-based confirmatory diagnostics
    M Kursad Araz
    Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA
    Lab Chip 13:3910-20. 2013
  7. doi Electrostatic protein immobilization using charged polyacrylamide gels and cationic detergent microfluidic Western blotting
    Dohyun Kim
    Department of Bioengineering, University of California, Berkeley, 342 Stanley Hall, Berkeley, California 94720, USA
    Anal Chem 84:2533-40. 2012
  8. doi Microfluidic validation of diagnostic protein markers for spontaneous cerebrospinal fluid rhinorrhea
    Akwasi A Apori
    Department of Bioengineering, University of California Berkeley, Berkeley, California, USA
    J Proteome Res 12:1254-65. 2013
  9. doi Post-collection processing of Schirmer strip-collected human tear fluid impacts protein content
    Aleksandra K Denisin
    Berkeley Bioengineering Department, University of California, Berkeley, 342 Stanley Hall, Berkeley, CA 94720 1762, USA
    Analyst 137:5088-96. 2012
  10. doi Chip-based immunoassays
    Akwasi A Apori
    Department of Bioengineering, University of California Berkeley, Berkeley, CA, USA
    Methods Mol Biol 919:233-48. 2013

Collaborators

  • Sanjay Kumar
  • Alex J Hughes
  • Akwasi A Apori
  • Todd A Duncombe
  • Augusto M Tentori
  • Kelly Karns
  • Rachel E Gerver
  • Chi Chih Kang
  • Samuel Q Tia
  • Dohyun Kim
  • Robert Lin
  • Yuchen Pan
  • Zhuchen Xu
  • Minsub Chung
  • M Kursad Araz
  • Aleksandra K Denisin
  • Daniel A Fletcher
  • Arunan Skandarajah
  • Hector D Neira
  • Jung Ming G Lin
  • Ming C Hammond
  • Colleen A Kellenberger
  • Dawn P Spelke
  • David V Schaffer
  • Cleo M Salisbury
  • Danica Chen
  • Katharine Brown
  • Martina N Brozynski
  • Ivan H El-Sayed
  • Donna M Peehl
  • Robert K C Lin
  • Mei He

Detail Information

Publications19

  1. doi A lateral electrophoretic flow diagnostic assay
    Robert Lin
    Department of Bioengineering, UC Berkeley, Berkeley, CA 94720 USA
    Lab Chip 15:1488-96. 2015
    ..The lateral e-flow assay provides a flexible format for conducting multiplexed immunoassays relevant to confirmatory diagnosis in near-patient settings. ..
  2. pmc Disruptive by design: a perspective on engineering in analytical chemistry
    Amy E Herr
    The UC Berkeley UCSF Graduate Program in Bioengineering, Berkeley, California, United States
    Anal Chem 85:7622-8. 2013
    ..The role of unmet needs identification, iteration in concept generation and development, and the existing gap in rapid prototyping tools for separations are all discussed. ..
  3. doi Photopatterned free-standing polyacrylamide gels for microfluidic protein electrophoresis
    Todd A Duncombe
    University of California, Berkeley University of California, San Francisco Graduate Program in Bioengineering, 342 Stanley Hall, Berkeley, California 94720, USA
    Lab Chip 13:2115-23. 2013
    ..Notably, the entire fsPAGE workflow from fabrication, to operation, and readout uses readily available materials and instruments - making this technique highly accessible...
  4. doi Protein post-translational modification analyses using on-chip immunoprobed isoelectric focusing
    Samuel Q Tia
    The UC Berkeley UCSF Graduate Program in Bioengineering, University of California, Berkeley, California 94720, United States
    Anal Chem 85:2882-90. 2013
    ..This new assay is well-matched to systems biology frameworks for study of protein post-translational modifications...
  5. pmc Microchamber integration unifies distinct separation modes for two-dimensional electrophoresis
    Augusto M Tentori
    The UC Berkeley UCSF Graduate Program in Bioengineering, Berkeley, California 94720, United States
    Anal Chem 85:4538-45. 2013
    ..The rapid microchamber 2DE assay has the potential to bridge an existing gap in targeted proteomics for protein biomarker validation and systems biology that may complement recent innovation in mass spectrometry...
  6. doi Microfluidic barcode assay for antibody-based confirmatory diagnostics
    M Kursad Araz
    Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA
    Lab Chip 13:3910-20. 2013
    ..Given the low voltage, low power and automated operation, we see the streamlined microfluidic barcode assay as a step towards rapid confirmatory diagnostics for a low-resource clinical laboratory setting. ..
  7. doi Electrostatic protein immobilization using charged polyacrylamide gels and cationic detergent microfluidic Western blotting
    Dohyun Kim
    Department of Bioengineering, University of California, Berkeley, 342 Stanley Hall, Berkeley, California 94720, USA
    Anal Chem 84:2533-40. 2012
    ..Using the microfluidic WB assay, assessment of lactoferrin in human tear fluid is demonstrated with a goal of advancing toward nonbiopsy-based diagnosis of Sjögren's Syndrome, an autoimmune disease...
  8. doi Microfluidic validation of diagnostic protein markers for spontaneous cerebrospinal fluid rhinorrhea
    Akwasi A Apori
    Department of Bioengineering, University of California Berkeley, Berkeley, California, USA
    J Proteome Res 12:1254-65. 2013
    ..The pilot study suggests the capability of a rapid on-chip validation tool to expedite scrutiny of putative protein markers for new clinical assays...
  9. doi Post-collection processing of Schirmer strip-collected human tear fluid impacts protein content
    Aleksandra K Denisin
    Berkeley Bioengineering Department, University of California, Berkeley, 342 Stanley Hall, Berkeley, CA 94720 1762, USA
    Analyst 137:5088-96. 2012
    ..Thus, we emphasize "spike and recover" control experiments to determine expected elution profiles for target proteins when using diffusion-based protein sample preparation for Schirmer strip-collected tear fluid...
  10. doi Chip-based immunoassays
    Akwasi A Apori
    Department of Bioengineering, University of California Berkeley, Berkeley, CA, USA
    Methods Mol Biol 919:233-48. 2013
    ....
  11. pmc Microfluidic integration for automated targeted proteomic assays
    Alex J Hughes
    Department of Bioengineering, and University of California, Berkeley University of California San Francisco Graduate Program in Bioengineering, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 109:5972-7. 2012
    ....
  12. pmc Single-cell Western blotting after whole-cell imaging to assess cancer chemotherapeutic response
    Chi Chih Kang
    Department of Bioengineering and the UC Berkeley UCSF Graduate Program in Bioengineering, University of California Berkeley, Berkeley, California 94720, United States
    Anal Chem 86:10429-36. 2014
    ..Accordingly, linking of phenotype with targeted protein analysis with single-cell resolution may advance our understanding of drug response in inherently heterogeneous cell populations, such as those anticipated in tumors. ..
  13. doi Microchamber Western blotting using poly-L-lysine conjugated polyacrylamide gel for blotting of sodium dodecyl sulfate coated proteins
    Minsub Chung
    Department of Bioengineering, University of California, Berkeley, California 94720, United States
    Anal Chem 85:7753-61. 2013
    ..The approach advances microfluidic protein immunoblotting, which is directly relevant to the widely-used SDS-PAGE based slab-gel Western blot, while saving sample volume, labor, and assay time...
  14. pmc Microfluidic Western blotting of low-molecular-mass proteins
    Rachel E Gerver
    University of California Berkeley and University of California San Francisco Graduate Program in Bioengineering, and Department of Bioengineering, University of California Berkeley, Berkeley, California 94720, United States
    Anal Chem 86:10625-32. 2014
    ..5 kDa, and a 100-fold better signal-to-noise ratio (SNR) for small pore gels, expanding the range of applicable biological targets. ..
  15. pmc High-throughput electrophoretic mobility shift assays for quantitative analysis of molecular binding reactions
    Yuchen Pan
    Graduate Program in Bioengineering, University of California San Francisco and University of California Berkeley, Berkeley, California 94720, United States
    Anal Chem 86:10357-64. 2014
    ....
  16. pmc Single-cell western blotting
    Alex J Hughes
    1 Department of Bioengineering, University of California UC Berkeley, Berkeley, California, USA 2 California Institute for Quantitative Biosciences, UC Berkeley, Berkeley, California, USA 3 4
    Nat Methods 11:749-55. 2014
    ..The scWestern overcomes limitations of antibody fidelity and sensitivity in other single-cell protein analysis methods and constitutes a versatile tool for the study of complex cell populations at single-cell resolution. ..
  17. doi Performance implications of chemical mobilization after microchannel IEF
    Augusto M Tentori
    The UC Berkeley UCSF Graduate Program in Bioengineering, Berkeley, CA, USA
    Electrophoresis 35:1453-60. 2014
    ....
  18. doi Use of polyacrylamide gel moving boundary electrophoresis to enable low-power protein analysis in a compact microdevice
    Todd A Duncombe
    University of California, Berkeley University of California, San Francisco Graduate Program in Bioengineering, 342 Stanley Hall, Berkeley, California 94720, United States
    Anal Chem 84:8740-7. 2012
    ..Looking forward, we see the low-power PAG MBE as a basis for highly multiplexed protein separations (mobility shift screening assays) as well as for portable low-power diagnostic assays...
  19. doi Human tear protein analysis enabled by an alkaline microfluidic homogeneous immunoassay
    Kelly Karns
    Graduate Program in Bioengineering, The University of California, Berkeley University of California, San Francisco, Berkeley, California 94720, United States
    Anal Chem 83:8115-22. 2011
    ....