Research Topics
Species | Jiang HeSummaryAffiliation: University of California Country: USA Publications
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Publications
Affinity enhancement pretargeting: synthesis and testing of a 99mTc-labeled bivalent MORFJiang He
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Mol Pharm 7:1118-24. 2010..Thus two monovalent MORFs were covalently conjugated into a bivalent MORF effector to improve tumor targeting by both pharmacokinetics and affinity enhancement influences...- Targeting prostate cancer cells in vivo using a rapidly internalizing novel human single-chain antibody fragmentJiang He
Department of Radiology and Biomedical Imaging, Center for Molecular and Functional Imaging, University of California at San Francisco, San Francisco, California 94143, USA
J Nucl Med 51:427-32. 2010..The objective of this study was to evaluate the tumor targeting of an internalizing human antibody fragment, a small-size platform, to provide high contrast in a mouse model of human prostate carcinoma... 
Amplification targeting: a modified pretargeting approach with potential for signal amplification-proof of a conceptJiang He
Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Nucl Med 45:1087-95. 2004..CONCLUSION: Although signal amplification has not yet been convincingly demonstrated and amplification targeting will require further studies for optimization, the concept has now been shown to be feasible...- Radiolabelling morpholinos with 188Re tricarbonyl provides improved in vitro and in vivo stability to re-oxidationJiang He
Division of Nuclear Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA
Nucl Med Commun 25:731-6. 2004..CONCLUSION: 188Re-tricarbonyl labelling of Hynic conjugated MORFs may be considerably more stable to oxidation than the MAG3 labelled MORFs and therefore more suitable for radiotherapy trials... - Radiolabeling of MAG3-morpholino oligomers with 188Re at high labeling efficiency and specific radioactivity for tumor pretargetingGuozheng Liu
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Appl Radiat Isot 64:971-8. 2006..A labeling efficiency of greater than 90% can be achieved even at a concentration of MAG(3)-cMORF as low as 0.8 microM. The labeled cMORF is stable and capable of hybridizing to its complement... - Pretargeting in tumored mice with radiolabeled morpholino oligomer showing low kidney uptakeGuozheng Liu
Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243, Worcester, USA
Eur J Nucl Med Mol Imaging 31:417-24. 2004..The lowering of kidney radioactivity levels in this way may be important to reduce toxicity to this organ in connection with pretargeting radiotherapy studies... - Predicting the biodistribution of radiolabeled cMORF effector in MORF-pretargeted miceGuozheng Liu
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0243, USA
Eur J Nucl Med Mol Imaging 34:237-46. 2007.... - Novel human single chain antibody fragments that are rapidly internalizing effectively target epithelioid and sarcomatoid mesotheliomasArun K Iyer
Center for Molecular and Functional Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California, USA
Cancer Res 71:2428-32. 2011.... - Improved delivery in cell culture of radiolabeled antisense DNAs by duplex formationXinrong Liu
Department of Radiology, Division of Nuclear Medicine, University of Massachusetts Medical School, Worcester, 01655, USA
Mol Imaging Biol 8:278-83. 2006..CONCLUSIONS: Targeting of mRNAs with radiolabeled AS DNAs may be improved in cell culture if duplexed with an S DNA engineered for low hybridization affinity to encourage dissociation in the presence of the target mRNA... - Further investigations of morpholino pretargeting in mice--establishing quantitative relations in tumorGuozheng Liu
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0243, USA
Eur J Nucl Med Mol Imaging 32:1115-23. 2005..In the present study, the pharmacokinetics of the antibody and effector were measured under different conditions in mice to establish their quantitative relationships with tumor accumulations by pretargeting... - Construction of a novel chimera consisting of a chelator-containing Tat peptide conjugated to a morpholino antisense oligomer for technetium-99m labeling and accelerating cellular kineticsYu Min Zhang
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Nucl Med Biol 33:263-9. 2006.... - Comparison of several linear fluorophore- and quencher-conjugated oligomer duplexes for stability, fluorescence quenching, and kinetics in vitro and in vivo in miceSurong Zhang
Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Bioconjug Chem 18:1170-5. 2007..5 may be efficiently and rapidly quenched by Iowa Black when both are conjugated to complementary oligomers and that the resulting inhibition of fluorescence is sufficiently persistent for imaging... - Successful radiotherapy of tumor in pretargeted mice by 188Re-radiolabeled phosphorodiamidate morpholino oligomer, a synthetic DNA analogueGuozheng Liu
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
Clin Cancer Res 12:4958-64. 2006..Our laboratory has reported on MORF pretargeting for diagnosis using (99m)Tc as radiolabel. We now report on the use of MORF pretargeting for radiotherapy in a mouse tumor model using (188)Re as the therapeutic radiolabel... - Optical pretargeting of tumor with fluorescent MORF oligomersJiang He
Department of Radiology, University of California San Francisco, San Francisco, CA 94143, USA
Mol Imaging Biol 9:17-23. 2007..The use of linear fluorophore-conjugated oligomer duplexes have been little used in animals, and to our knowledge, have not previously been considered for pretargeting applications... - Evaluation of 99mTc-MAG3-annexin V: influence of the chelate on in vitro and in vivo properties in miceJean Luc Vanderheyden
Department of Radiology, University of Massachusetts Medical School, Worcester, 01655, USA
Nucl Med Biol 33:135-44. 2006..Nevertheless, (99m)Tc MAG(3)-annexin may be an attractive alternative to (99m)Tc Hynic-annexin for the in vivo imaging of phosphatidylserine receptors... - The effect of internalizing human single chain antibody fragment on liposome targeting to epithelioid and sarcomatoid mesotheliomaArun K Iyer
Center for Molecular and Functional Imaging, Department of Radiology and Biomedical Imaging, University of California at San Francisco, San Francisco, CA 94143, USA
Biomaterials 32:2605-13. 2011.... - Initial observations of 99mTc labelled locked nucleic acids for antisense targetingYumin Zhang
Department of Radiology, University of Massachusetts Medical School, Worcester, MA, USA
Nucl Med Commun 25:1113-8. 2004..CONCLUSIONS: LNAs appear to be attractive oligomers for antisense targeting and other radiopharmaceutical applications... - Pharmacokinetics in mice of four oligomer-conjugated polymers for amplification targetingJiang He
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA, USA
Cancer Biother Radiopharm 18:941-7. 2003..CONCLUSION: The biodistribution of the four polymers showed characteristic differences, with one polymer (30 KDa PL) showing the most favorable properties for amplification targeting... - Affinity enhancement bivalent morpholinos for pretargeting: surface plasmon resonance studies of molecular dimensionsJiang He
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Bioconjug Chem 16:1098-104. 2005..The results provide further evidence that the use of bivalent effectors can greatly improve MORF pretargeting and, finally, that bivalent MORFs with reduced equilibrium constants may actually exhibit higher affinity enhancement... - Cytosine residues influence kidney accumulations of 99mTc-labeled morpholino oligomersGuozheng Liu
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Antisense Nucleic Acid Drug Dev 12:393-8. 2002..These results show that the base sequences of MORFs, and probably other oligomers as well, are an important determinant of biodistribution... - An improved method for covalently conjugating morpholino oligomers to antitumor antibodiesJiang He
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Bioconjug Chem 18:983-8. 2007..Using MN14(SANH)-MORF, the influence of the higher gpm on pretargeting permitted lowering the dosages of MN14 administered and may permit administering higher levels of radioactivity in connection with therapy... - A comparison of in vitro and in vivo stability in mice of two morpholino duplexes differing in chain lengthJiang He
Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Bioconjug Chem 14:1018-23. 2003..By contrast, homoduplex prepared with (99m)Tc-MORF25 showed higher stability. These differences in hybridization stability may be important considerations in radiopharmaceutical design... - Initial mechanistic studies of antisense targeting in cellsXinrong Liu
Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Nucl Med 47:360-8. 2006..If the target is primarily pre-mRNA in the nucleus rather than mature mRNA in the cytoplasm, this would provide as well an explanation for the observed migration of 99mTc-labeled antisense DNA into the nucleus... - Affinity enhancement bivalent morpholino for pretargeting: initial evidence by surface plasmon resonanceJiang He
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Bioconjug Chem 16:338-45. 2005..These results show that bimolecular binding was occurring in the case of the bivalent MORF and suggest that bivalency may be superior to monovalency in MORF pretargeting applications... - Preparation and properties of 99mTc(CO)3+-labeled N,N-bis(2-pyridylmethyl)-4-aminobutyric acidGuozheng Liu
Department of Radiology, Division of Nuclear Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Bioconjug Chem 15:1441-6. 2004..Finally, the complex (99m)Tc(CO)(3)(+)-BPABA itself was found to clear exclusively via the hepatobiliary pathway and may have value as a potential hepatobiliary imaging agent... - Biodistributions of 177Lu- and 111In-labeled 7E11 antibodies to prostate-specific membrane antigen in xenograft model of prostate cancer and potential use of 111In-7E11 as a pre-therapeutic agent for 177Lu-7E11 radioimmunotherapyMei Hsiu Pan
Center for Molecular and Functional Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA 94143, USA
Mol Imaging Biol 11:159-66. 2009..To serve as a radioimmunotherapeutic agent, a kinetically inert conjugate is desired to maximize tumor uptake and tumor radiation dose with minimal nonspecific exposure to bone marrow and other major organs... - Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesotheliomaScott Bidlingmaier
Department of Anesthesia, San Francisco General Hospita, San Francisco, California, USA
Cancer Res 69:1570-7. 2009.... - Radiolabeling morpholinos with 90Y, 111In, 188Re and 99mTcChang-bin Liu
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA, USA
Nucl Med Biol 30:207-14. 2003..These strategies for labeling MORF with (90)Y and (188)Re therefore appear to be suitable for therapeutic applications although both show some evidence of instabilities... - Electrostatic binding with tat and other cationic peptides increases cell accumulation of 99mTc-antisense DNAs without entrapmentYu-Min Zhang
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Mol Imaging Biol 5:240-7. 2003..In addition, at least a fraction of the transducted DNA appears to be free of entrapment to hybridize to its mRNA target in live cells... - In vitro cell studies of technetium-99m labeled RGD-HYNIC peptide, a comparison of tricine and EDDA as co-ligandsZi-Fen Su
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Nucl Med Biol 30:141-9. 2003.... - Development of an optimized activatable MMP-14 targeted SPECT imaging probeGregory A Watkins
Center for Molecular and Functional Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, 185 Berry Street, Suite 350, Box 0946, San Francisco, CA 94107, United States
Bioorg Med Chem 17:653-9. 2009..The combination of computational chemistry, parallel synthesis and biochemical screening, therefore, shows promise as a set of tools for developing new radiolabeled probes that are sensitive to protease activity... - Symptoms characteristic of heart failure among CKD patients without diagnosed heart failureMichael G Shlipak
University of California, San Francisco, General Internal Medicine, San Francisco VA Medical Center, 4150 Clement St, 111A1, San Francisco, CA 94109, USA
J Card Fail 17:17-23. 2011..Epidemiological studies typically diagnose heart failure (HF) at the time of hospitalization, and have not evaluated the prevalence of HF symptoms in CKD patients without a prior HF diagnosis... - Improving the labeling of S-acetyl NHS-MAG(3)-conjugated morpholino oligomersGuozheng Liu
Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Bioconjug Chem 13:893-7. 2002.... 
Association of N-terminal pro-B-type natriuretic peptide with left ventricular structure and function in chronic kidney disease (from the Chronic Renal Insufficiency Cohort [CRIC])Rakesh K Mishra
University of California, San Francisco, USA
Am J Cardiol 111:432-8. 2013..10, 95% CI 0.10 to 0.11; p = 0.07). In conclusion, in this large chronic kidney disease cohort without heart failure, NT-proBNP had strong associations with prevalent LVH and LV systolic dysfunction...
Research Grants
- Targeted liposomal radiotherapy of malignant mesotheliomaJiang He; Fiscal Year: 2010..If successfully completed, this novel method will create far-reaching applications in RIT, particularly for high-potent treatments of malignant mesothelioma. ..