G F Hatfull

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Fluoromycobacteriophages for rapid, specific, and sensitive antibiotic susceptibility testing of Mycobacterium tuberculosis
    Mariana Piuri
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 4:e4870. 2009
  2. pmc Comparative genomic analysis of mycobacteriophage Tweety: evolutionary insights and construction of compatible site-specific integration vectors for mycobacteria
    Thuy T Pham
    Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Microbiology 153:2711-23. 2007
  3. pmc A peptidoglycan hydrolase motif within the mycobacteriophage TM4 tape measure protein promotes efficient infection of stationary phase cells
    Mariana Piuri
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 62:1569-85. 2006
  4. pmc Growth of Mycobacterium tuberculosis biofilms containing free mycolic acids and harbouring drug-tolerant bacteria
    Anil K Ojha
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 69:164-74. 2008
  5. pmc BRED: a simple and powerful tool for constructing mutant and recombinant bacteriophage genomes
    Laura J Marinelli
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS ONE 3:e3957. 2008
  6. pmc The role of iron in Mycobacterium smegmatis biofilm formation: the exochelin siderophore is essential in limiting iron conditions for biofilm formation but not for planktonic growth
    Anil Ojha
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 66:468-83. 2007
  7. pmc Cluster J mycobacteriophages: intron splicing in capsid and tail genes
    Welkin H Pope
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 8:e69273. 2013
  8. pmc Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination
    Shweta Singh
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS Genet 9:e1003490. 2013
  9. pmc Cluster K mycobacteriophages: insights into the evolutionary origins of mycobacteriophage TM4
    Welkin H Pope
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e26750. 2011
  10. pmc Expanding the diversity of mycobacteriophages: insights into genome architecture and evolution
    Welkin H Pope
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e16329. 2011

Research Grants

  1. MOLECULAR BIOLOGY OF MYCOBACTERIA AND THEIR PHAGES
    Graham Hatfull; Fiscal Year: 2004
  2. Mycobacteriophage-mediated Recombineering in Mycobacterium tuberculosis
    Graham Hatfull; Fiscal Year: 2007
  3. Integration and excision by serine-integrases
    Graham Hatfull; Fiscal Year: 2004
  4. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 2000
  5. Phage mimicry of mycobacterial signaling
    Graham Hatfull; Fiscal Year: 2006
  6. INTEGRATION AND EXCISION OF PROPHAGES IN TUBERCULOSIS
    Graham Hatfull; Fiscal Year: 2001
  7. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 1999
  8. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 2002
  9. Integration and excision by serine-integrases
    Graham Hatfull; Fiscal Year: 2005
  10. Phage mimicry of mycobacterial signaling
    Graham Hatfull; Fiscal Year: 2007

Collaborators

Detail Information

Publications65

  1. pmc Fluoromycobacteriophages for rapid, specific, and sensitive antibiotic susceptibility testing of Mycobacterium tuberculosis
    Mariana Piuri
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 4:e4870. 2009
    ..Fluorescence withstands fixation by paraformaldehyde providing enhanced biosafety for testing MDR-TB and XDR-TB infections...
  2. pmc Comparative genomic analysis of mycobacteriophage Tweety: evolutionary insights and construction of compatible site-specific integration vectors for mycobacteria
    Thuy T Pham
    Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Microbiology 153:2711-23. 2007
    ....
  3. pmc A peptidoglycan hydrolase motif within the mycobacteriophage TM4 tape measure protein promotes efficient infection of stationary phase cells
    Mariana Piuri
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 62:1569-85. 2006
    ..Tmp's of mycobacteriophages may thus have acquired these motifs in order to avoid a selective disadvantage that results from changes in peptidoglycan in non-growing cells...
  4. pmc Growth of Mycobacterium tuberculosis biofilms containing free mycolic acids and harbouring drug-tolerant bacteria
    Anil K Ojha
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 69:164-74. 2008
    ....
  5. pmc BRED: a simple and powerful tool for constructing mutant and recombinant bacteriophage genomes
    Laura J Marinelli
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS ONE 3:e3957. 2008
    ..We describe the use of BRED to construct unmarked gene deletions, in-frame internal deletions, base substitutions, precise gene replacements, and the addition of gene tags...
  6. pmc The role of iron in Mycobacterium smegmatis biofilm formation: the exochelin siderophore is essential in limiting iron conditions for biofilm formation but not for planktonic growth
    Anil Ojha
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 66:468-83. 2007
    ..M. smegmatis surface motility is similarly dependent on iron availability, requiring both supplemental iron and the exochelin pathway to acquire it...
  7. pmc Cluster J mycobacteriophages: intron splicing in capsid and tail genes
    Welkin H Pope
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 8:e69273. 2013
    ..The unusual Cluster J genomes provide new insights into phage genome architecture, gene function, capsid structure, gene mobility, intron splicing, and evolution. ..
  8. pmc Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination
    Shweta Singh
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS Genet 9:e1003490. 2013
    ..The opposing effect of site identifiers ensures that site schizophrenia with dual identities does not occur...
  9. pmc Cluster K mycobacteriophages: insights into the evolutionary origins of mycobacteriophage TM4
    Welkin H Pope
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e26750. 2011
    ..The K1 phages integrate into the host tmRNA and the Cluster K phages represent potential new tools for the genetics of M. tuberculosis and related species...
  10. pmc Expanding the diversity of mycobacteriophages: insights into genome architecture and evolution
    Welkin H Pope
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e16329. 2011
    ..The isolation and genomic analysis of bacteriophages by freshman college students provides an example of an authentic research experience for novice scientists...
  11. pmc Mycobacteriophage endolysins: diverse and modular enzymes with multiple catalytic activities
    Kimberly M Payne
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e34052. 2012
    ..Most contain three domains with a novel N-terminal predicted peptidase, a centrally located amidase, muramidase, or transglycosylase, and a C-terminal putative cell wall binding domain...
  12. pmc Bacteriophages and their genomes
    Graham F Hatfull
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Curr Opin Virol 1:298-303. 2011
    ..But we have barely scratched the surface and the next years of phage genome exploration promise to be especially revealing...
  13. pmc Mycobacteriophage Marvin: a new singleton phage with an unusual genome organization
    Catherine Mageeney
    Cabrini College, Department of Science, Radnor, Pennsylvania, USA
    J Virol 86:4762-75. 2012
    ....
  14. pmc Complete genome sequences of 138 mycobacteriophages
    Graham F Hatfull
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Virol 86:2382-4. 2012
    ....
  15. pmc Phamerator: a bioinformatic tool for comparative bacteriophage genomics
    Steven G Cresawn
    Department of Biology, James Madison University, 820 Madison Dr, MSC 7801, Harrisonburg, VA 22807 USA
    BMC Bioinformatics 12:395. 2011
    ..Bacteriophage genomes have mosaic architectures and are replete with small open reading frames of unknown function, presenting challenges in their annotation, comparative analysis, and representation...
  16. pmc Comparative genomics of the mycobacteriophages: insights into bacteriophage evolution
    Graham F Hatfull
    Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Res Microbiol 159:332-9. 2008
    ....
  17. ncbi request reprint Mycobacteriophages: genes and genomes
    Graham F Hatfull
    Pittsburgh Bacteriophage Institute, Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Annu Rev Microbiol 64:331-56. 2010
    ..Mycobacteriophages have provided key tools for tuberculosis genetics, and new methods for simple construction of mycobacteriophage recombinants will facilitate postgenomic explorations into mycobacteriophage biology...
  18. pmc Exploring the mycobacteriophage metaproteome: phage genomics as an educational platform
    Graham F Hatfull
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS Genet 2:e92. 2006
    ....
  19. pmc Bacteriophage genomics
    Graham F Hatfull
    Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Curr Opin Microbiol 11:447-53. 2008
    ..With an estimated 10(31) bacterial and archael viruses in the biosphere, our view of the virosphere will draw into sharper focus as further bacteriophage genomes are characterized...
  20. pmc Comparative genomic analysis of 60 Mycobacteriophage genomes: genome clustering, gene acquisition, and gene size
    Graham F Hatfull
    Department of Biological Sciences, Pittsburgh Bacteriophage Institute, Pittsburgh, PA 15260, USA
    J Mol Biol 397:119-43. 2010
    ....
  21. pmc Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage
    R W Hendrix
    Pittsburgh Bacteriophage Institute, Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Proc Natl Acad Sci U S A 96:2192-7. 1999
    ....
  22. pmc Control of directionality in integrase-mediated recombination: examination of recombination directionality factors (RDFs) including Xis and Cox proteins
    J A Lewis
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Nucleic Acids Res 29:2205-16. 2001
    ..This compilation will prove useful in recognizing new proteins that confer directionality upon site-specific recombination reactions encoded by plasmids, transposons, phages and prophages...
  23. ncbi request reprint Superinfection immunity of mycobacteriophage L5: applications for genetic transformation of mycobacteria
    M K Donnelly-Wu
    Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260
    Mol Microbiol 7:407-17. 1993
    ....
  24. ncbi request reprint Origins of highly mosaic mycobacteriophage genomes
    Marisa L Pedulla
    Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Cell 113:171-82. 2003
    ..While the role of phages as vehicles of toxin genes is well established, these observations suggest a much broader involvement of phages in bacterial virulence and the host response to bacterial infections...
  25. ncbi request reprint Mycobacteriophage TM4: genome structure and gene expression
    M E Ford
    Pittsburgh Bacteriophage Institute, USA
    Tuber Lung Dis 79:63-73. 1998
    ..Understanding the genetics of TM4 will greatly facilitate its use as a tool for the genetic manipulation of the mycobacteria...
  26. pmc Control of phage Bxb1 excision by a novel recombination directionality factor
    Pallavi Ghosh
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS Biol 4:e186. 2006
    ....
  27. pmc Characterization of the dnaG locus in Mycobacterium smegmatis reveals linkage of DNA replication and cell division
    A G Klann
    Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA
    J Bacteriol 180:65-72. 1998
    ....
  28. ncbi request reprint The origins and ongoing evolution of viruses
    R W Hendrix
    Pittsburgh Bacteriophage Institute, PA 15260, USA
    Trends Microbiol 8:504-8. 2000
    ..These processes suggest a model for early virus evolution, wherein viruses can be regarded less as having derived from cells and more as being partners in their mutual co-evolution...
  29. ncbi request reprint DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics
    G F Hatfull
    Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260
    Mol Microbiol 7:395-405. 1993
    ....
  30. pmc Mycobacteriophages BPs, Angel and Halo: comparative genomics reveals a novel class of ultra-small mobile genetic elements
    Timothy Sampson
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Microbiology 155:2962-77. 2009
    ..The attP site is located within the repressor-coding region, such that prophage formation results in expression of a C-terminally truncated, but active, form of the repressor...
  31. pmc Exponential-phase glycogen recycling is essential for growth of Mycobacterium smegmatis
    A E Belanger
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Bacteriol 181:6670-8. 1999
    ..Thus, in addition to its conventional storage role, glycogen may also serve as a carbon capacitor for glycolysis during the exponential growth of M. smegmatis...
  32. pmc Mycobacteriophage Lysin B is a novel mycolylarabinogalactan esterase
    Kimberly Payne
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 73:367-81. 2009
    ..We propose that LysB facilitates lysis by compromising the integrity of the mycobacterial outer membrane linkage to the arabinogalactan-peptidoglycan layer...
  33. pmc Enzymatic hydrolysis of trehalose dimycolate releases free mycolic acids during mycobacterial growth in biofilms
    Anil K Ojha
    Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 285:17380-9. 2010
    ..tuberculosis, suggesting the presence of a TDM-specific esterase in this pathogen. Overall, this study provides the first evidence for an enzymatic release of free mycolic acids from cell envelope mycolates during mycobacterial growth...
  34. pmc Genomic characterization of mycobacteriophage Giles: evidence for phage acquisition of host DNA by illegitimate recombination
    Peter Morris
    Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Bacteriol 190:2172-82. 2008
    ..In a second example, a DNA segment has been recently acquired from the host bacterial chromosome by illegitimate recombination, providing further evidence that phage genomic mosaicism is generated by nontargeted recombination processes...
  35. pmc Imbroglios of viral taxonomy: genetic exchange and failings of phenetic approaches
    Jeffrey G Lawrence
    Department of Biological Sciences, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Bacteriol 184:4891-905. 2002
    ..For bacteriophages, such dissections of genomic sequences reveal fundamental flaws in the Linnaean paradigm that necessitate a new view of viral evolution, classification, and taxonomy...
  36. ncbi request reprint Bacteriophages with tails: chasing their origins and evolution
    Roger W Hendrix
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Res Microbiol 154:253-7. 2003
    ..Extrapolation of these processes back in evolutionary time leads to a speculative model for the origins and early evolution of phages...
  37. doi request reprint Mycobacterial recombineering
    Julia C van Kessel
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Biol 435:203-15. 2008
    ..Mycobacterial recombineering, therefore, offers a simple approach for constructing gene replacement mutants in M. smegmatis and M. tuberculosis...
  38. ncbi request reprint GroEL1: a dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria
    Anil Ojha
    Pittsburgh Bacteriophage Institute, Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Cell 123:861-73. 2005
    ....
  39. ncbi request reprint Recombineering in Mycobacterium tuberculosis
    Julia C van Kessel
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, 376 Crawford Hall, 4249 Fifth Ave, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Nat Methods 4:147-52. 2007
    ..smegmatis and M. tuberculosis. Mycobacterial recombineering thus provides a simple approach for the construction of gene replacement mutants in both slow- and fast-growing mycobacteria...
  40. ncbi request reprint Mycobacteriophage Bxb1 integrates into the Mycobacterium smegmatis groEL1 gene
    Amy I Kim
    Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 50:463-73. 2003
    ..Establishment of a defined in vitro system for Bxb1 integration shows that recombination occurs efficiently without requirement for high-energy cofactors, divalent metals, DNA supercoiling or additional proteins...
  41. doi request reprint Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets
    Julia C van Kessel
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, 376 Crawford Hall, 4249 Fifth Ave, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 67:1094-107. 2008
    ....
  42. ncbi request reprint Integration and excision by the large serine recombinase phiRv1 integrase
    Lori A Bibb
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 55:1896-910. 2005
    ....
  43. ncbi request reprint Integration and excision of the Mycobacterium tuberculosis prophage-like element, phiRv1
    Lori A Bibb
    Department of Biological Sciences, University of Pittsburgh, PA 15260, USA
    Mol Microbiol 45:1515-26. 2002
    ..Integration-proficient plasmid vectors derived from phiRv1 efficiently transform BCG, can utilize four of the seven REP13E12 sites present in BCG as attachment sites, and can occupy more than one site simultaneously...
  44. ncbi request reprint The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB
    Pallavi Ghosh
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Cell 12:1101-11. 2003
    ....
  45. ncbi request reprint Microbiology. A tail of two specifi-cities
    Graham F Hatfull
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Science 295:2031-2. 2002
  46. ncbi request reprint Synapsis in phage Bxb1 integration: selection mechanism for the correct pair of recombination sites
    Pallavi Ghosh
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Mol Biol 349:331-48. 2005
    ....
  47. ncbi request reprint Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus
    Gregory J Morgan
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Mol Biol 317:337-59. 2002
    ..This is reminiscent of the arrangement established for phage lambda...
  48. pmc Corrected sequence of the bacteriophage p22 genome
    Marisa L Pedulla
    Pittsburgh Bacteriophage Institute Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Bacteriol 185:1475-7. 2003
    ..DNA sequencing technology is now good enough that genomes of important model systems like P22 can be sequenced with essentially 100% accuracy with minimal investment of time and resources...
  49. pmc Two-step site selection for serine-integrase-mediated excision: DNA-directed integrase conformation and central dinucleotide proofreading
    Pallavi Ghosh
    Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Proc Natl Acad Sci U S A 105:3238-43. 2008
    ....
  50. pmc Recombineering mycobacteria and their phages
    Julia C van Kessel
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Nat Rev Microbiol 6:851-7. 2008
    ..Exploitation of host-specific phages thus provides a general strategy for recombineering and mutagenesis in genetically naive systems...
  51. ncbi request reprint Control of directionality in L5 integrase-mediated site-specific recombination
    John A Lewis
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Mol Biol 326:805-21. 2003
    ..The mechanism of action of Xis-L5 appears to be purely architectural, influencing the assembly of protein-DNA structures solely through its DNA-binding and DNA-bending properties...
  52. pmc The generalized transducing Salmonella bacteriophage ES18: complete genome sequence and DNA packaging strategy
    Sherwood R Casjens
    Department of Pathology, University of Utah Medical School, Salt Lake City, UT 84132, USA
    J Bacteriol 187:1091-104. 2005
    ..Bioinformatic analysis of large terminase subunits shows that the different functional classes of phage-encoded terminases can usually be predicted from their amino acid sequence...
  53. pmc Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase
    Louis J Nkrumah
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nat Methods 3:615-21. 2006
    ..falciparum life cycle, and it illustrates the utility of Bxb1-based integrative recombination for genetic studies of intracellular eukaryotic organisms...
  54. ncbi request reprint Inquiry learning. Teaching scientific inquiry
    David I Hanauer
    Graduate Program in Composition and TESOL, Department of English, Indiana University of Pennsylvania, Indiana, PA 15705, USA
    Science 314:1880-1. 2006
  55. ncbi request reprint Genomic and structural analysis of Syn9, a cyanophage infecting marine Prochlorococcus and Synechococcus
    Peter R Weigele
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Environ Microbiol 9:1675-95. 2007
    ..These genes suggest that Syn9 is well adapted to the physiology of its photosynthetic hosts and may affect the evolution of these sequences within marine cyanobacteria...
  56. ncbi request reprint Mycobacteriophage exploit NHEJ to facilitate genome circularization
    Robert S Pitcher
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, UK
    Mol Cell 23:743-8. 2006
    ..These results establish a new paradigm for sequestration of the host cell NHEJ process by bacteriophage and provide a framework for understanding similar transactions in eukaryotic viral infections...
  57. ncbi request reprint Bxz1, a new generalized transducing phage for mycobacteria
    Sunhee Lee
    Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    FEMS Microbiol Lett 241:271-6. 2004
    ..smegmatis. We also demonstrated cotransduction of a transposon insertion linked to a point mutation of the ndh gene...
  58. pmc Genome sequence, structural proteins, and capsid organization of the cyanophage Syn5: a "horned" bacteriophage of marine synechococcus
    Welkin H Pope
    Department of Biology, Massachusetts Institute of Technology, Cambridge MA 02139, USA
    J Mol Biol 368:966-81. 2007
    ..This phage/host system should allow detailed investigation of the physiology and biochemistry of phage propagation in marine photosynthetic bacteria...
  59. ncbi request reprint Rapid identification and susceptibility testing of Mycobacterium tuberculosis from MGIT cultures with luciferase reporter mycobacteriophages
    Niaz Banaiee
    Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, CA, USA
    J Med Microbiol 52:557-61. 2003
    ..LRPs offer an accurate and rapid approach for identification and susceptibility testing of M. tuberculosis from MGIT-960 cultures...
  60. pmc The pKO2 linear plasmid prophage of Klebsiella oxytoca
    Sherwood R Casjens
    Department of Pathology, University of Utah Medical School, Salt Lake City, Utah 84132, USA
    J Bacteriol 186:1818-32. 2004
    ..We show that these divergently transcribed genes are regulated by LexA protein binding to a single target site that overlaps both promoters...
  61. pmc Complete genomic sequence of the virulent Salmonella bacteriophage SP6
    Aleisha T Dobbins
    Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, DC 20059, USA
    J Bacteriol 186:1933-44. 2004
    ..Bioinformatic analysis of the SP6 sequence strongly suggested that DNA replication occurs in large part through a bidirectional mechanism, possibly with circular intermediates...
  62. ncbi request reprint Exploring the prokaryotic virosphere
    André M Comeau
    Laboratoire de Microbiologie et Genetique Moleculaires, Centre National de la Recherche Scientifique, Université Paul Sabatier Toulouse III UMR5100, Toulouse, France
    Res Microbiol 159:306-13. 2008
    ..In this review, we highlight some of the directions where we believe research on the prokaryotic virosphere will lead us in the near future...

Research Grants30

  1. MOLECULAR BIOLOGY OF MYCOBACTERIA AND THEIR PHAGES
    Graham Hatfull; Fiscal Year: 2004
    ..We will also use proteomic approaches to understand the influence of phage gene expression on that of its host. ..
  2. Mycobacteriophage-mediated Recombineering in Mycobacterium tuberculosis
    Graham Hatfull; Fiscal Year: 2007
    ..These putative recombination proteins will be functionally and biochemically characterized and used to develop recombineering systems in both fast- and slow-growing mycobacteria. ..
  3. Integration and excision by serine-integrases
    Graham Hatfull; Fiscal Year: 2004
    ..These studies will provide important insights into how phage integration and excision operate and how they can be harnessed as genetical tools for understanding M. tuberculosis. ..
  4. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 2000
    ..In addition to Providing new insights into the mechanism of recombination these studies will offer new tools for mycobacterial genetics and a greater understanding of mycobacterial physiology. ..
  5. Phage mimicry of mycobacterial signaling
    Graham Hatfull; Fiscal Year: 2006
    ..We propose to elucidate the specific role of GroEL1 in biofilm formation and mycolic acid synthesis, and to determine its role in the pathogenesis of Mycobacterium tuberculosis. ..
  6. INTEGRATION AND EXCISION OF PROPHAGES IN TUBERCULOSIS
    Graham Hatfull; Fiscal Year: 2001
    ..tuberculoses to determine whether they have functional recombination systems; whether they act as Genetic Transfer Agents; and whether they play a role in virulence of M. tuberculosis. ..
  7. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 1999
    ..In addition to Providing new insights into the mechanism of recombination these studies will offer new tools for mycobacterial genetics and a greater understanding of mycobacterial physiology. ..
  8. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 2002
    ..In addition to Providing new insights into the mechanism of recombination these studies will offer new tools for mycobacterial genetics and a greater understanding of mycobacterial physiology. ..
  9. Integration and excision by serine-integrases
    Graham Hatfull; Fiscal Year: 2005
    ..These studies will provide important insights into how phage integration and excision operate and how they can be harnessed as genetical tools for understanding M. tuberculosis. ..
  10. Phage mimicry of mycobacterial signaling
    Graham Hatfull; Fiscal Year: 2007
    ..We propose to elucidate the specific role of GroEL1 in biofilm formation and mycolic acid synthesis, and to determine its role in the pathogenesis of Mycobacterium tuberculosis. ..
  11. Integration and Excision by Serine Intergrases
    Graham F Hatfull; Fiscal Year: 2010
    ..Recombination is a core biological process and understanding its mechanisms and regulation will have a broad impact on our understanding of biology and medicine. ..
  12. Phage mimicry of mycobacterial signaling
    Graham F Hatfull; Fiscal Year: 2010
    ..We propose to elucidate the specific role of GroEL1 in biofilm formation and mycolic acid synthesis, and to determine its role in the pathogenesis of Mycobacterium tuberculosis. ..
  13. MOLECULAR BIOLOGY OF MYCOBACTERIA AND THEIR PHAGES
    Graham Hatfull; Fiscal Year: 2001
    ..We will also use proteomic approaches to understand the influence of phage gene expression on that of its host. ..
  14. MOLECULAR BIOLOGY OF MYCOBACTERIA AND THEIR PHAGES
    Graham Hatfull; Fiscal Year: 2002
    ..We will also use proteomic approaches to understand the influence of phage gene expression on that of its host. ..
  15. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 2001
    ..In addition to Providing new insights into the mechanism of recombination these studies will offer new tools for mycobacterial genetics and a greater understanding of mycobacterial physiology. ..
  16. Integration and excision by serine-integrases
    Graham Hatfull; Fiscal Year: 2006
    ..These studies will provide important insights into how phage integration and excision operate and how they can be harnessed as genetical tools for understanding M. tuberculosis. ..
  17. Phage mimicry of mycobacterial signaling
    Graham Hatfull; Fiscal Year: 2009
    ..We propose to elucidate the specific role of GroEL1 in biofilm formation and mycolic acid synthesis, and to determine its role in the pathogenesis of Mycobacterium tuberculosis. ..
  18. INTEGRATION AND EXCISION OF PROPHAGES IN TUBERCULOSIS
    Graham Hatfull; Fiscal Year: 2002
    ..tuberculoses to determine whether they have functional recombination systems; whether they act as Genetic Transfer Agents; and whether they play a role in virulence of M. tuberculosis. ..
  19. INTEGRATION AND EXCISION OF PROPHAGES IN TUBERCULOSIS
    Graham Hatfull; Fiscal Year: 1999
    ..tuberculoses to determine whether they have functional recombination systems; whether they act as Genetic Transfer Agents; and whether they play a role in virulence of M. tuberculosis. ..
  20. Integration and excision by serine-integrases
    Graham Hatfull; Fiscal Year: 2007
    ..These studies will provide important insights into how phage integration and excision operate and how they can be harnessed as genetical tools for understanding M. tuberculosis. ..
  21. MOLECULAR BIOLOGY OF MYCOBACTERIA AND THEIR PHAGES
    Graham Hatfull; Fiscal Year: 1999
    ..These studies will contribute to the construction of sensitive and specific diagnostic reporter phages, tools for construction of recombinant mycobacterial vaccines and systems for analyzing mycobacterial gene expression. ..
  22. INTEGRATION AND EXCISION OF PROPHAGES IN TUBERCULOSIS
    Graham Hatfull; Fiscal Year: 2000
    ..tuberculoses to determine whether they have functional recombination systems; whether they act as Genetic Transfer Agents; and whether they play a role in virulence of M. tuberculosis. ..
  23. INTEGRATION AND EXCISION OF PROPHAGES IN TUBERCULOSIS
    Graham Hatfull; Fiscal Year: 2003
    ..tuberculoses to determine whether they have functional recombination systems; whether they act as Genetic Transfer Agents; and whether they play a role in virulence of M. tuberculosis. ..
  24. MOLECULAR BIOLOGY OF MYCOBACTERIA AND THEIR PHAGES
    Graham Hatfull; Fiscal Year: 2003
    ..We will also use proteomic approaches to understand the influence of phage gene expression on that of its host. ..