Research Topics
| DANIEL HASSETTSummaryAffiliation: University of Cincinnati Country: USA Publications
Research Grants
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Detail Information
Publications
Quorum sensing in Pseudomonas aeruginosa controls expression of catalase and superoxide dismutase genes and mediates biofilm susceptibility to hydrogen peroxideD J Hassett
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Bethesda Avenue, Cincinnati, OH 45257 0524, USA
Mol Microbiol 34:1082-93. 1999....
Pseudomonas aeruginosa hypoxic or anaerobic biofilm infections within cystic fibrosis airwaysDaniel J Hassett
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
Trends Microbiol 17:130-8. 2009..Mutants lacking these factors might be uniquely susceptible to nitrogen oxide, specifically the nitrite anion (NO(2)(-)), in the treatment of P. aeruginosa airway infections in CF...- Bactericidal antibiotics and oxidative stress: a radical proposalDaniel J Hassett
Department of Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45221, USA
ACS Chem Biol 2:708-10. 2007..This proposal represents an abrupt departure from conventional models, and it will attract further experimental tests... - Pseudomonas aeruginosa biofilm infections in cystic fibrosis: insights into pathogenic processes and treatment strategiesDaniel J Hassett
University of Cincinnati College of Medicine, Department of Molecular Genetics, 231 Albert Sabin Way, Cincinnati, OH 45267 0524, USA
Expert Opin Ther Targets 14:117-30. 2010..Better understanding of the mechanisms used by P. aeruginosa to circumvent host defenses and therapeutic intervention strategies is critical for advancing novel treatment strategies... - Anaerobic metabolism and quorum sensing by Pseudomonas aeruginosa biofilms in chronically infected cystic fibrosis airways: rethinking antibiotic treatment strategies and drug targetsDaniel J Hassett
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0524, USA
Adv Drug Deliv Rev 54:1425-43. 2002..aeruginosa for more effective treatment of chronic CF lung infections... 
Hydrogen peroxide sensitivity of catechol-2,3-dioxygenase: a cautionary note on use of xylE reporter fusions under aerobic conditionsD J Hassett
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0524, USA
Appl Environ Microbiol 66:4119-23. 2000..Our results suggest using caution in interpreting data derived from xylE reporter fusions under aerobic conditions, especially where oxidative stress is present or when catalase-deficient strains are used...- A protease-resistant catalase, KatA, released upon cell lysis during stationary phase is essential for aerobic survival of a Pseudomonas aeruginosa oxyR mutant at low cell densitiesD J Hassett
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0524, USA
J Bacteriol 182:4557-63. 2000..Taken together, our results indicate that inactivation of OxyR renders P. aeruginosa exquisitely sensitive to both H(2)O(2) and aminoglycosides, which are clinically and environmentally important antimicrobials... - Anaerobic killing of mucoid Pseudomonas aeruginosa by acidified nitrite derivatives under cystic fibrosis airway conditionsSang Sun Yoon
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0524, USA
J Clin Invest 116:436-46. 2006..In summary, selective killing by HNO2 may provide novel insights into the important clinical goal of eradicating mucoid P. aeruginosa from the CF airways... - Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respirationSang Sun Yoon
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0524, USA
EMBO J 26:3662-72. 2007..These data suggest that protection of a P. aeruginosa norCB mutant against anaerobic NO toxicity occurs by both control of NO supply and reassignment of metabolic enzymes to the task of NO sequestration... - Pseudomonas aeruginosa pyocyanin is critical for lung infection in miceGee W Lau
Division of Pulmonary and Critical Care Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, MSB 6006, Cincinnati, OH 45267 0564, USA
Infect Immun 72:4275-8. 2004..Here, by using two respiratory tract models of infection, we demonstrate that PCN mediates tissue damage and necrosis during lung infection... - Chronic Pseudomonas aeruginosa infection in cystic fibrosis airway disease: metabolic changes that unravel novel drug targetsSang Sun Yoon
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, OH 45267 0524, USA
Expert Rev Anti Infect Ther 2:611-23. 2004..Finally, a new hypothesis, that P. aeruginosa thrives as biofilms within the thickened anaerobic mucus layers, will be developed... - Modulation of lung epithelial functions by Pseudomonas aeruginosaGee W Lau
Department of Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0557, USA
Trends Microbiol 13:389-97. 2005..Understanding the complex interactions between the lung epithelium and P. aeruginosa might enable more effective therapeutic strategies against infection in cystic fibrosis and immuno-compromised individuals... - Pseudomonas aeruginosa OxyR is required for full virulence in rodent and insect models of infection and for resistance to human neutrophilsGee W Lau
Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0524, USA
Infect Immun 73:2550-3. 2005 - The role of pyocyanin in Pseudomonas aeruginosa infectionGee W Lau
Division of Pulmonary and Critical Care Medicine, and Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0564, USA
Trends Mol Med 10:599-606. 2004..aeruginosa during infection. In addition, few cellular pathways that are affected by PCN are known. This review briefly highlights recent advances that might clarify the role of PCN in P. aeruginosa pathogenesis... - Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesisCharles C Caldwell
Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
Am J Pathol 175:2473-88. 2009..In summary, this study establishes that PCN is an important P. aeruginosa virulence factor capable of directly inducing pulmonary pathophysiology in mice, consistent with changes observed in CF and other bronchiectasis lungs... - Pseudomonas aeruginosa anaerobic respiration in biofilms: relationships to cystic fibrosis pathogenesisSang Sun Yoon
Department of Molecular Genetics, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
Dev Cell 3:593-603. 2002..Thus, future investigations of CF pathogenesis and therapy should include a better understanding of anaerobic metabolism and biofilm development by P. aeruginosa... - Differential roles of OxyR-controlled antioxidant enzymes alkyl hydroperoxide reductase (AhpCF) and catalase (KatB) in the protection of Pseudomonas aeruginosa against hydrogen peroxide in biofilm vs. planktonic cultureWarunya Panmanee
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA
FEMS Microbiol Lett 295:238-44. 2009..planktonic culture upon exposure to hydrogen peroxide. AhpCF was found to be critical for survival of biofilm bacteria while KatB was more important for survival of planktonic free-swimming organisms... - Human targets of Pseudomonas aeruginosa pyocyaninHuimin Ran
Division of Pulmonary and Critical Care Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0564, USA
Proc Natl Acad Sci U S A 100:14315-20. 2003..We discuss how the inactivation of V-ATPase may negatively impact the lung function of cystic fibrosis patients... - The peptidoglycan-associated lipoprotein OprL helps protect a Pseudomonas aeruginosa mutant devoid of the transactivator OxyR from hydrogen peroxide-mediated killing during planktonic and biofilm cultureWarunya Panmanee
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0524, USA
J Bacteriol 190:3658-69. 2008.... - NKG2D is critical for NK cell activation in host defense against Pseudomonas aeruginosa respiratory infectionScott C Wesselkamper
Department of Environmental Health, Division of Environmental Genetics and Molecular Toxicology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
J Immunol 181:5481-9. 2008..aeruginosa respiratory infection... - A zinc-dependent adhesion module is responsible for intercellular adhesion in staphylococcal biofilmsDeborah G Conrady
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0524, USA
Proc Natl Acad Sci U S A 105:19456-61. 2008..Zn(2+) chelation represents a potential therapeutic approach for combating biofilm growth in a wide range of bacterial biofilm-related infections... - Global analysis of cellular factors and responses involved in Pseudomonas aeruginosa resistance to arseniteKislay Parvatiyar
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, OH 45267-0524, USA
J Bacteriol 187:4853-64. 2005..SOD appears to be involved, but its function may be in the protection of superoxide-sensitive sulfhydryl groups... - Pseudomonas aeruginosa pyocyanin inactivates lung epithelial vacuolar ATPase-dependent cystic fibrosis transmembrane conductance regulator expression and localizationFansheng Kong
Division of Pulmonary and Critical Care Medicine, Cincinnati Children's Hospital, Cincinnati, OH, USA
Cell Microbiol 8:1121-33. 2006..Our study supports the notion that PCN contributes significantly to the pathogenesis of CF and other bronchiectasis patients infected by PA... - Gallium disrupts iron uptake by intracellular and extracellular Francisella strains and exhibits therapeutic efficacy in a murine pulmonary infection modelOyebode Olakanmi
Medical Service, VA Medical Center Cincinnati, Cincinnati, Ohio 45220, USA
Antimicrob Agents Chemother 54:244-53. 2010..These data support the potential utility of Ga as a therapy for F. tularensis infection of the lung... - Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditionsMark D Platt
Department of Chemistry, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
J Bacteriol 190:2739-58. 2008..aeruginosa to limit the growth of this important CF pathogen when it is either limited or completely restricted in its oxygen supply... - Pseudomonas aeruginosa AlgR represses the Rhl quorum-sensing system in a biofilm-specific mannerLisa A Morici
Tulane University Health Sciences Center, Department of Microbiology and Immunology, New Orleans, LA 70112, USA
J Bacteriol 189:7752-64. 2007..These data also suggest that AlgR may control transcription in a contact-dependent or biofilm-specific manner... - Static growth of mucoid Pseudomonas aeruginosa selects for non-mucoid variants that have acquired flagellum-dependent motilityTimna J O Wyckoff
Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157-1064, USA
Microbiology 148:3423-30. 2002..This may afford P. aeruginosa the ability to swim towards areas of higher oxygen concentrations. Subsequent to this, algT mutations are likely to secure the motile phenotype... - Involvement of nitric oxide in biofilm dispersal of Pseudomonas aeruginosaNicolas Barraud
School of Biotechnology and Biomolecular Sciences and Centre for Marine Biofouling and Bio-Innovation, Biological Sciences Building, University of New South Wales, Kensington, Sydney, NSW 2052, Australia
J Bacteriol 188:7344-53. 2006..aeruginosa biofilms from a glass surface. Combined exposure to both NO and antimicrobial agents may therefore offer a novel strategy to control preestablished, persistent P. aeruginosa biofilms and biofilm-related infections... - Transcriptome analysis of Pseudomonas aeruginosa after interaction with human airway epithelial cellsAnders Frisk
Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, Louisiana Center for Lung Biology and Immunotherapy, Tulane University Health Sciences Center, New Orleans, LA 70112-2699, USA
Infect Immun 72:5433-8. 2004..Gene expression profiles indicated that various genes involved in phosphate acquisition and iron scavenging were differentially regulated... - The transcriptional regulator AlgR controls cyanide production in Pseudomonas aeruginosaAlexander J Carterson
Department of Microbiology and Immunology, Tulane University Health Sciences Center, 1430 Tulane Ave, New Orleans, LA 70112-2699, USA
J Bacteriol 186:6837-44. 2004..aeruginosa CF isolates are more hypercyanogenic than nonmucoid laboratory strains. Taken together, cyanide production may be a relevant virulence factor in CF lung disease, the production of which is regulated, in part, by AlgR... - The mobA gene is required for assimilatory and respiratory nitrate reduction but not xanthine dehydrogenase activity in Pseudomonas aeruginosaChris Noriega
Department of Biology, University of Dayton, Dayton, OH 45469, USA
Curr Microbiol 51:419-24. 2005.... - The exopolysaccharide alginate protects Pseudomonas aeruginosa biofilm bacteria from IFN-gamma-mediated macrophage killingJeff G Leid
Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA
J Immunol 175:7512-8. 2005..aeruginosa biofilm bacteria from the human immune system... - BdlA, a chemotaxis regulator essential for biofilm dispersion in Pseudomonas aeruginosaRyan Morgan
Department of Biological Sciences, Binghamton University, SUNY at Binghamton, 104 Science III, NY 13902, USA
J Bacteriol 188:7335-43. 2006..Our findings suggest that BdlA may be a link between sensing environmental cues, c-di-GMP levels, and detachment. Based on our findings, a possible involvement of BdlA in a signaling cascade resulting in biofilm dispersion is discussed... - The Pseudomonas aeruginosa type IV pilin receptor binding domain functions as an adhesin for both biotic and abiotic surfacesCarmen L Giltner
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
Mol Microbiol 59:1083-96. 2006..Therefore, the C-terminal receptor binding domain appears to have evolved for binding a variety of surfaces... - Subinhibitory bismuth-thiols reduce virulence of Pseudomonas aeruginosaChieh-Liang Wu
CardioPulmonary Research Institute, Division of Pulmonary and Critical Care Medicine, Winthrop-University Hospital, SUNY School of Medicine at Stony Brook, Mineola, New York 11501, USA
Am J Respir Cell Mol Biol 26:731-8. 2002..In summary, low BT concentrations affect P. aeruginosa in a variety of ways, the combination of which may help prevent or resolve respiratory tract infection... - Cytotoxicity of Pseudomonas secreted exotoxins requires OxyR expressionKurt A Melstrom
Department of Surgery, Loyola University Medical Center, Maywood, Illinois 60153, USA
J Surg Res 143:50-7. 2007..Pseudomonas aeruginosa combats host-initiated oxidant stress through expression of the transactivating factor, OxyR. Here we have tested the premise that OxyR regulates Pseudomonal cytotoxicity through secreted exotoxin production... - MglA regulates transcription of virulence factors necessary for Francisella tularensis intraamoebae and intramacrophage survivalCrystal M Lauriano
Department of Microbiology and Immunology, University of Texas Health Science Center, San Antonio, TX 78229-3900, USA
Proc Natl Acad Sci U S A 101:4246-9. 2004..Thus, MglA regulates the transcription of virulence factors of F. tularensis that contribute to intramacrophage and intraamoebae survival... - Bacterial biofilms of importance to medicine and bioterrorism: proteomic techniques to identify novel vaccine components and drug targetsDaniel J Hassett
Expert Opin Biol Ther 3:1201-7. 2003..The other is referred to as persistent 'bioterrorist agent biofilms' in which Francisella tularensis can grow on surfaces where environmental amoeba can phagocytose them, allowing for growth of F. tularensis within the amoebae... - A Na+:H+ antiporter and a molybdate transporter are essential for arsenite oxidation in Agrobacterium tumefaciensDes R Kashyap
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, Montana 59717, USA
J Bacteriol 188:1577-84. 2006..In each mutant, the loss of As(III)-oxidizing capacity resulted in conversion to an arsenate [As(V)]-reducing phenotype. Neither mutant was more sensitive to As(III) than the parental strain...
Research Grants
- B.pseudomallei bioterrorism and quorum sensingDANIEL HASSETT; Fiscal Year: 2003..These studies will provide important information that will lead to countermeasures against a potential bioterrorist attack using the potentially lethal gram-negative bacterium, B. pseudomallei. ..
- Proteogenome of Anaerobic P. aeruginosa in CF MucusDANIEL HASSETT; Fiscal Year: 2003..Our LONG TERM GOAL is to identify cellular targets that inhibit or significantly compromise the denitrification process that is occurring by P. aeruginosa in the CF airways. ..
- IRON, FUR AND ALGINATE PRODUCTION IN P AERUGINOSADANIEL HASSETT; Fiscal Year: 2002..Information derived from the studies proposed herein will advance the current knowledge about the role of these factors in the pathogenesis of P. aeruginosa infections in CF. ..
- Role of OxyR in P. aeruginosa Biofilm Resistance to H202DANIEL HASSETT; Fiscal Year: 2007....