Steven D Harris

Summary

Affiliation: University of Nebraska
Country: USA

Publications

  1. ncbi request reprint Branching of fungal hyphae: regulation, mechanisms and comparison with other branching systems
    Steven D Harris
    Department of Plant Pathology and Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska 68588, USA
    Mycologia 100:823-32. 2008
  2. pmc SepBCTF4 is required for the formation of DNA-damage-induced UvsCRAD51 foci in Aspergillus nidulans
    Scott E Gygax
    Department of Microbiology, University of Connecticut Health Center, Farmington, 06030 3205, USA
    Genetics 169:1391-402. 2005
  3. doi request reprint Morphogenetic and developmental functions of the Aspergillus nidulans homologues of the yeast bud site selection proteins Bud4 and Axl2
    Haoyu Si
    Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Mol Microbiol 85:252-70. 2012
  4. pmc Regulation of septum formation by the Bud3-Rho4 GTPase module in Aspergillus nidulans
    Haoyu Si
    Department of Plant Pathology and Center for Plant Science Innovation, University of Nebraska, Lincoln, NE 68588 0660, USA
    Genetics 185:165-76. 2010
  5. ncbi request reprint Induction of cell wall thickening by the antifungal compound dihydromaltophilin disrupts fungal growth and is mediated by sphingolipid biosynthesis
    Shaojie Li
    Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68583, USA
    J Eukaryot Microbiol 56:182-7. 2009
  6. ncbi request reprint Regulation of hyphal morphogenesis by cdc42 and rac1 homologues in Aspergillus nidulans
    Aleksandra Virag
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Mol Microbiol 66:1579-96. 2007
  7. ncbi request reprint Cell polarity in filamentous fungi: shaping the mold
    Steven D Harris
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588, USA
    Int Rev Cytol 251:41-77. 2006
  8. doi request reprint Characterization of Fusarium graminearum Mes1 reveals roles in cell-surface organization and virulence
    William R Rittenour
    Department of Plant Pathology and Plant Science Initiative, University of Nebraska Lincoln, N234 Beadle Center, Lincoln, NE 68588 0660, USA
    Fungal Genet Biol 45:933-46. 2008
  9. doi request reprint Methods to detect apoptotic-like cell death in filamentous fungi
    Camile P Semighini
    Department of Plant Pathology and Center for Plant Science Innovation, University of Nebraska, Lincoln, NE, USA
    Methods Mol Biol 638:269-79. 2010
  10. pmc Functional characterization of Aspergillus nidulans homologues of Saccharomyces cerevisiae Spa2 and Bud6
    Aleksandra Virag
    Plant Science Initiative, and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Eukaryot Cell 5:881-95. 2006

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Branching of fungal hyphae: regulation, mechanisms and comparison with other branching systems
    Steven D Harris
    Department of Plant Pathology and Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska 68588, USA
    Mycologia 100:823-32. 2008
    ..e., the Spitzenkorper, septins) are discussed. Finally, other forms of branching in the plant and animal kingdoms are briefly summarized and compared to hyphal branching...
  2. pmc SepBCTF4 is required for the formation of DNA-damage-induced UvsCRAD51 foci in Aspergillus nidulans
    Scott E Gygax
    Department of Microbiology, University of Connecticut Health Center, Farmington, 06030 3205, USA
    Genetics 169:1391-402. 2005
    ..We also provide evidence that implicates SepB in sister-chromatid cohesion, thereby suggesting that cohesion may play a role in regulating the localization and/or assembly of UvsC(RAD51) complexes...
  3. doi request reprint Morphogenetic and developmental functions of the Aspergillus nidulans homologues of the yeast bud site selection proteins Bud4 and Axl2
    Haoyu Si
    Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Mol Microbiol 85:252-70. 2012
    ..nidulans, but also in the phylogenetically unrelated fungus Fusarium graminearum. Our results suggest that Axl2 plays a key role in phialide morphogenesis and/or function during conidiation in the aspergilli...
  4. pmc Regulation of septum formation by the Bud3-Rho4 GTPase module in Aspergillus nidulans
    Haoyu Si
    Department of Plant Pathology and Center for Plant Science Innovation, University of Nebraska, Lincoln, NE 68588 0660, USA
    Genetics 185:165-76. 2010
    ..Our observations provide new insight into the signaling pathways that regulate septum formation in filamentous fungi...
  5. ncbi request reprint Induction of cell wall thickening by the antifungal compound dihydromaltophilin disrupts fungal growth and is mediated by sphingolipid biosynthesis
    Shaojie Li
    Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68583, USA
    J Eukaryot Microbiol 56:182-7. 2009
    ..The thickened cell wall could be digested by chitinase suggesting that chitin contributes to the HSAF-induced thickening. Furthermore, HSAF treatment activated the transcription of two chitin synthase encoding genes chsB and chsC...
  6. ncbi request reprint Regulation of hyphal morphogenesis by cdc42 and rac1 homologues in Aspergillus nidulans
    Aleksandra Virag
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Mol Microbiol 66:1579-96. 2007
    ..Our results are consistent with the action of parallel Cdc42 and microtubule-based pathways in regulating the formation of a stable axis of hyphal polarity in A. nidulans...
  7. ncbi request reprint Cell polarity in filamentous fungi: shaping the mold
    Steven D Harris
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588, USA
    Int Rev Cytol 251:41-77. 2006
    ..Moreover, the stabilization of nascent polarity axes may be the critical step that permits the emergence of a new hypha...
  8. doi request reprint Characterization of Fusarium graminearum Mes1 reveals roles in cell-surface organization and virulence
    William R Rittenour
    Department of Plant Pathology and Plant Science Initiative, University of Nebraska Lincoln, N234 Beadle Center, Lincoln, NE 68588 0660, USA
    Fungal Genet Biol 45:933-46. 2008
    ..Our results provide a basis for identifying determinants of fungal virulence that may localize to specialized domains at the cell surface...
  9. doi request reprint Methods to detect apoptotic-like cell death in filamentous fungi
    Camile P Semighini
    Department of Plant Pathology and Center for Plant Science Innovation, University of Nebraska, Lincoln, NE, USA
    Methods Mol Biol 638:269-79. 2010
    ..We also present approaches for monitoring nuclear condensation and production of ROS...
  10. pmc Functional characterization of Aspergillus nidulans homologues of Saccharomyces cerevisiae Spa2 and Bud6
    Aleksandra Virag
    Plant Science Initiative, and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Eukaryot Cell 5:881-95. 2006
    ..We propose that the core function of the polarisome in polarized growth is maintained but with different contributions of polarisome components to the process...
  11. pmc Functional characterization of the putative Aspergillus nidulans poly(ADP-ribose) polymerase homolog PrpA
    Camile P Semighini
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln 68588 0660, USA
    Genetics 173:87-98. 2006
    ..Collectively, our results provide a gateway for probing the diverse functions of PARP in a sophisticated microbial genetic system...
  12. pmc MesA, a novel fungal protein required for the stabilization of polarity axes in Aspergillus nidulans
    Claire L Pearson
    Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06030 3205, USA
    Mol Biol Cell 15:3658-72. 2004
    ..We also provide evidence that MesA may regulate the formation or distribution of sterol-rich membrane domains. Our results suggest that these domains may be part of novel mechanism that directs SepA to hyphal tips...
  13. doi request reprint An in vitro method for the analysis of infection-related morphogenesis in Fusarium graminearum
    William R Rittenour
    Department of Plant Pathology and Center for Plant Science Innovation, University of Nebraska, E126 Beadle Center, Lincoln, NE 68588 0660, USA
    Mol Plant Pathol 11:361-9. 2010
    ..This relatively simple assay will contribute to the tractability of the F. graminearum system and help to uncover molecular requirements for infection-related development...
  14. doi request reprint The Spitzenkörper: a signalling hub for the control of fungal development?
    Steven D Harris
    Department of Plant Pathology, University of Nebraska, Lincoln, NE 68688 0660, USA
    Mol Microbiol 73:733-6. 2009
    ..Notably, these studies suggest that the Spitzenkörper (i.e. apical body), which is known to regulate hyphal morphogenesis, might also serve as a signalling hub that co-ordinates development transitions...
  15. ncbi request reprint Characterization of the Aspergillus nidulans 14-3-3 homologue, ArtA
    Peter R Kraus
    Department of Microbiology, University of Connecticut Health Center, Farmington 06030 3205, USA
    FEMS Microbiol Lett 210:61-6. 2002
    ..However, instead of blocking septation, over-expression of artA causes a severe delay in the polarization of conidiospores. This observation suggests that these proteins play an important role in hyphal morphogenesis...
  16. doi request reprint Inhibition of Fusarium graminearum growth and development by farnesol
    Camile P Semighini
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska Lincoln, Lincoln, NE 68588, USA
    FEMS Microbiol Lett 279:259-64. 2008
    ..Given the economic importance of F. graminearum as a pathogen of small grains, this study proposes that farnesol may have potential value as an antifungal compound...
  17. pmc Regulation of apical dominance in Aspergillus nidulans hyphae by reactive oxygen species
    Camile P Semighini
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68588 0660, USA
    Genetics 179:1919-32. 2008
    ..Collectively, our results support a model whereby localized Nox activity generates a pool of ROS that defines a dominant polarity axis at hyphal tips...
  18. ncbi request reprint Polarity in filamentous fungi: moving beyond the yeast paradigm
    Steven D Harris
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, NE, USA
    Fungal Genet Biol 41:391-400. 2004
    ....
  19. pmc Evolutionary aspects of urea utilization by fungi
    Dhammika H M L P Navarathna
    School of Biological Sciences, University of Nebraska, Lincoln, NE 68588 0666, USA
    FEMS Yeast Res 10:209-13. 2010
    ..Also, the absence of MccA in the hemiascomycetes coincides with and may explain their production of fusel alcohols...
  20. pmc Distinct ceramide synthases regulate polarized growth in the filamentous fungus Aspergillus nidulans
    Shaojie Li
    Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Mol Biol Cell 17:1218-27. 2006
    ....
  21. pmc Polarisome meets spitzenkörper: microscopy, genetics, and genomics converge
    Steven D Harris
    Plant Science Initiative, Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Eukaryot Cell 4:225-9. 2005
  22. pmc Molecular evolution of urea amidolyase and urea carboxylase in fungi
    Pooja K Strope
    School of Biological Sciences, University of Nebraska, Lincoln, NE 68588, USA
    BMC Evol Biol 11:80. 2011
    ..In order to elucidate the evolutionary origin of urea amidolyase and urea carboxylase, we studied the distribution of urea amidolyase, urea carboxylase, as well as other proteins including urease, across kingdoms...
  23. doi request reprint Cdc42/Rho GTPases in fungi: variations on a common theme
    Steven D Harris
    Department of Plant Pathology and Center for Plant Science Innovation, University of Nebraska, Lincoln, NE 68688 0660, USA
    Mol Microbiol 79:1123-7. 2011
    ..Surprisingly, the partitioning of these roles between Cdc42 and Rac1 seems to vary even among related fungi. These observations highlight the variable use of a common signalling module in filamentous fungi...
  24. doi request reprint Hyphal morphogenesis: an evolutionary perspective
    Steven D Harris
    Center for Plant Science Innovation and Department of Plant Pathology, University of Nebraska, E126 Beadle Center, Lincoln, NE 68506, USA
    Fungal Biol 115:475-84. 2011
    ..e., yeast vs. hyphae) using an essentially identical inventory of gene products are also considered. Finally, processes implicated in the regulation of hyphal tip complexes are addressed from an evolutionary perspective...
  25. ncbi request reprint Morphology and development in Aspergillus nidulans: a complex puzzle
    Steven D Harris
    Department of Plant Pathology and Center for Plant Science Innovation, University of Nebraska, E126 Beadle Center, Lincoln, NE 68588 0660, USA
    Fungal Genet Biol 46:S82-S92. 2009
    ..nidulans genes encode proteins that may participate at some level in morphogenesis during vegetative growth and development...
  26. ncbi request reprint Farnesol-induced apoptosis in Aspergillus nidulans reveals a possible mechanism for antagonistic interactions between fungi
    Camile P Semighini
    Plant Science Initiative, University of Nebraska Lincoln, Lincoln, NE, USA
    Mol Microbiol 59:753-64. 2006
    ..albicans. Taken together, our data suggest that farnesol, in addition to its quorum-sensing function that regulates morphogenesis, is also employed by C. albicans to reduce competition from other microbes...
  27. pmc Structure and biosynthesis of heat-stable antifungal factor (HSAF), a broad-spectrum antimycotic with a novel mode of action
    Fengan Yu
    Department of Chemistry, University of Nebraska Lincoln, 729 Hamilton Hall, Lincoln, NE 68588 0304, USA
    Antimicrob Agents Chemother 51:64-72. 2007
    ..The elucidation of the chemical structure of HSAF and the identification of the genetic locus for its biosynthesis establish the foundation for future exploitation of this group of compounds as new fungicides or antifungal drugs...
  28. ncbi request reprint Morphogenesis in germinating Fusarium graminearum macroconidia
    Steven D Harris
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68588 0660, USA
    Mycologia 97:880-7. 2005
    ..These observations suggest that F. graminearum possesses a regulatory system that marks germ tube emergence sites. Perturbation of this system may represent an effective approach for inhibiting colonization of host plant surfaces...
  29. pmc Functional characterization and localization of the Aspergillus nidulans formin SEPA
    Kathryn E Sharpless
    Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06030 3205, USA
    Mol Biol Cell 13:469-79. 2002
    ..We propose that specific morphological cues activate common molecular pathways to direct SEPA localization to the appropriate morphogenetic site...
  30. ncbi request reprint The Spitzenkörper: a molecular perspective
    Aleksandra Virag
    Plant Science Initiative, University of Nebraska Lincoln, 1901 Vine Street, Lincoln, NE 68588 0660, USA
    Mycol Res 110:4-13. 2006
    ..Morphogenesis of such highly polarized cells is complex, and requires the coordinated action of multiple protein complexes. We discuss the relevance of these complexes for the structure and function of the Spitzenkörper...
  31. ncbi request reprint Functional characterization of the Aspergillus fumigatus calcineurin
    Márcia Eliana da Silva Ferreira
    Faculdade de Ciências Farmacêuticas de Ribeirão, Universidade de Sao Paulo, Brazil
    Fungal Genet Biol 44:219-30. 2007
    ..These results suggest that calcineurin is involved in the regulation of the PHO pathway in A. fumigatus. Our work on calcineurin opens new venues for the research on sensing and nutrient acquisition in A. fumigatus...
  32. pmc Regulation of hyphal morphogenesis and the DNA damage response by the Aspergillus nidulans ATM homolog AtmA
    Iran Malavazi
    Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Sao Paulo, Brazil
    Genetics 173:99-109. 2006
    ..Our results suggest that AtmA regulates the function and/or localization of landmark proteins required for the formation of a polarity axis. We propose that a similar function may contribute to the establishment of neuronal polarity...
  33. pmc Aspergillus nidulans uvsBATR and scaANBS1 genes show genetic interactions during recovery from replication stress and DNA damage
    Marcia Regina von Zeska Kress Fagundes
    Departamento de Ciencias Farmaceuticas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av do Cafe S N, CEP 14040 903, Ribeirao Preto, Sao Paulo, Brazil
    Eukaryot Cell 4:1239-52. 2005
    ..Our results highlight the extensive genetic interactions between UvsB and the Mre11 complex that are required for S-phase progression and recovery from DNA damage...
  34. pmc Identification of a topoisomerase I mutant, scsA1, as an extragenic suppressor of a mutation in scaA(NBS1), the apparent homolog of human nibrin in Aspergillus nidulans
    Marcia R Z Kress Fagundes
    Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, CEP 14040 903 São Paulo, Brazil
    Genetics 164:935-45. 2003
    ..The scsA(TOP1) and the scaA(NBS1) gene products could functionally interact in pathways that either monitor or repair DNA double-strand breaks...
  35. pmc Transcriptome analysis of Aspergillus nidulans exposed to camptothecin-induced DNA damage
    Iran Malavazi
    Departamento de Ciencias Farmaceuticas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av do Cafe S N, CEP 14040 903, Ribeirao Preto, Sao Paulo, Brazil
    Eukaryot Cell 5:1688-704. 2006
    ..Our results provide the first insight into the overall complexity of the response to DNA damage in filamentous fungi and suggest that multiple pathways may act in parallel to mediate DNA repair...
  36. ncbi request reprint The DNA damage response in filamentous fungi
    Gustavo H Goldman
    Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Sao Paulo, Brazil
    Fungal Genet Biol 35:183-95. 2002
    ..Further characterization of this response will likely yield general insights that are applicable to animals and plants. Moreover, it may also become evident that the DNA damage response can be manipulated to control fungal growth...