Research Topics
Species | E HarrisSummaryAffiliation: University of California Country: USA Publications
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Publications
A mouse model for studying dengue virus pathogenesis and immune responseKatherine L Williams
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California Berkeley, Berkeley, California 94720 7354, USA
Ann N Y Acad Sci 1171:E12-23. 2009..This review provides a brief overview of the development of a mouse model of DENV infection, describes the work completed to date characterizing the AG129 model, and examines several of the unanswered questions remaining in the field...
Clinical, epidemiologic, and virologic features of dengue in the 1998 epidemic in NicaraguaE Harris
Division of Infectious Diseases, School of Public Health, University of California, Berkeley 94720 7360, USA
Am J Trop Med Hyg 63:5-11. 2000..As evidenced by the analysis of this epidemic, the epidemiology of dengue can differ according to geographic region and viral serotype...- Rapid subtyping of dengue viruses by restriction site-specific (RSS)-PCRE Harris
Division of Public Health Biology and Epidemiology, University of California, Berkeley, California, 94720, USA
Virology 253:86-95. 1999..This novel PCR approach to subtyping organisms based on restriction site polymorphisms should be applicable to other pathogens... 
Fluid intake and decreased risk for hospitalization for dengue fever, NicaraguaEva Harris
University of California, Berkeley, California 94720 6350, USA
Emerg Infect Dis 9:1003-6. 2003..Similar results were obtained for children <15 years of age and older adolescents and adults in independent analyses...- Building scientific capacity in developing countriesEva Harris
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California, USA
EMBO Rep 5:7-11. 2004 - Typing of dengue viruses in clinical specimens and mosquitoes by single-tube multiplex reverse transcriptase PCRE Harris
Program in Molecular Pathogenesis, University of California, San Francisco, San Francisco, California 94143 0422, USA
J Clin Microbiol 36:2634-9. 1998..This assay should serve as a practical tool for use in countries where dengue fever is endemic, in conjunction with classical methods for surveillance and epidemiology of dengue viruses... - Interferon inhibits dengue virus infection by preventing translation of viral RNA through a PKR-independent mechanismM S Diamond
Division of Infectious Diseases, University of California, Berkeley, Berkeley, California 94720 7360, USA
Virology 289:297-311. 2001..We conclude that IFN prevents DV infection by inhibiting translation of the infectious viral RNA through a novel, PKR-independent mechanism... - Nested restriction site-specific PCR to detect and type hepatitis C virus (HCV): a rapid method to distinguish HCV subtype 1b from other genotypesL Krekulova
Division of Infectious Diseases, School of Public Health, University of California-Berkeley, Berkeley, CA 94720, USA
J Clin Microbiol 39:1774-80. 2001..Its simplicity and speed may provide new opportunities to study the epidemiology of HCV infections and the relationship between HCV genotypes and clinical outcome by more laboratories throughout the world... - Y box-binding protein-1 binds to the dengue virus 3'-untranslated region and mediates antiviral effectsSuman Marie Paranjape
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720 7360, USA
J Biol Chem 282:30497-508. 2007..These results suggest a novel role for YB-1 as an antiviral host cell factor... - RNA secondary structure in the coding region of dengue virus type 2 directs translation start codon selection and is required for viral replicationKaren Clyde
Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California, Berkeley, 94720 7360, USA
J Virol 80:2170-82. 2006..While numerous regulatory elements have been identified in the untranslated regions of RNA viral genomes, we show that the cHP is a coding-region RNA element that directs start codon selection and is required for viral replication... - The capsid-coding region hairpin element (cHP) is a critical determinant of dengue virus and West Nile virus RNA synthesisKaren Clyde
Division of Infectious Diseases and Immunity, School of Public Health, 1 Barker Hall, University of California, Berkeley, CA 94720 7354, USA
Virology 379:314-23. 2008..We propose that the cHP is a multifunctional determinant of flavivirus replication, functioning in both translation and RNA synthesis... - Inhibition of dengue virus translation and RNA synthesis by a morpholino oligomer targeted to the top of the terminal 3' stem-loop structureKatherine Lynn Holden
Division of Infectious Diseases, School of Public Health, University of California at Berkeley, 140 Warren Hall, Berkeley, CA 94720-7360, USA
Virology 344:439-52. 2006..These results show that the 3'CS and the 3'SL domains regulate DEN translation and RNA synthesis and further demonstrate that P-PMOs are potentially useful as antiviral agents... - Interplay of RNA elements in the dengue virus 5' and 3' ends required for viral RNA replicationPeter Friebe
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, 1 Barker Hall, Berkeley, CA 94720 7354, USA
J Virol 84:6103-18. 2010..We also show that the UAR, DAR, cHP, and CS must act in concert and therefore likely function together to form the tertiary RNA structure of the circularized DENV genome... - The 5' and 3' downstream AUG region elements are required for mosquito-borne flavivirus RNA replicationPeter Friebe
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California 94720 7354, USA
J Virol 85:1900-5. 2011..Using West Nile virus as a model, we demonstrate that 5'-3' DAR complementarity and HP-3'SL formation are essential for viral RNA replication... - Clinical attack rate and presentation of pandemic H1N1 influenza versus seasonal influenza A and B in a pediatric cohort in NicaraguaAubree Gordon
Divisions of Infectious Diseases and Vaccinology, University of California, Berkeley, USA
Clin Infect Dis 50:1462-7. 2010..The clinical influenza attack rate was high in both pandemic and seasonal years... - Global spread and persistence of dengueJennifer L Kyle
Division of Infectious Diseases, School of Public Health, and Graduate Group in Microbiology, University of California, Berkeley, California 94720 7354, USA
Annu Rev Microbiol 62:71-92. 2008..What is known, as well as gaps in knowledge, is emphasized in light of future prospects for control and prevention of this pandemic disease... - Dengue virus infects macrophages and dendritic cells in a mouse model of infectionJennifer L Kyle
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720 7360, USA
J Infect Dis 195:1808-17. 2007..We find that the initial cellular tropism of DENV in mice is similar to that reported in humans, thereby paving the way for investigation of cellular tropism and pathogenesis of DENV in primary and secondary infections... - Immunoglobulin M enzyme-linked immunosorbent assay using recombinant polypeptides for diagnosis of dengueElsa Videa
Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California, Berkeley, CA 94720-7360, USA
Clin Diagn Lab Immunol 12:882-4. 2005.... - Dengue virus utilizes a novel strategy for translation initiation when cap-dependent translation is inhibitedDianna Edgil
Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California, Berkeley, Berkeley, California 94720-7360, USA
J Virol 80:2976-86. 2006..This DEN noncanonical translation is not mediated by an internal ribosome entry site but requires the interaction of the DEN 5' and 3' UTRs for activity, suggesting a novel strategy for translation of animal viruses... - Translation efficiency determines differences in cellular infection among dengue virus type 2 strainsDianna Edgil
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720-7360, USA
Virology 317:275-90. 2003..Thus, differences in infectivity among closely related DEN2 strains correlate with efficiency of translation of input viral RNA... - Prevalence and seasonality of influenza-like illness in children, Nicaragua, 2005-2007Aubree Gordon
School of Public Health, University of California, Berkeley, California 94720 7354, USA
Emerg Infect Dis 15:408-14. 2009..Influenza virus circulation was confirmed during each ILI peak by laboratory testing of a subset of samples. Our findings demonstrate a high rate of ILI, with seasonal peaks, in children in Nicaragua... - Infection of human cells by dengue virus is modulated by different cell types and viral strainsM S Diamond
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
J Virol 74:7814-23. 2000..Thus, depending on the cell type and viral isolate, blocks that limit the production of infectious virus in vitro may occur at distinct steps in the pathway of cellular infection... - Characterization of Aedes aegypti (Diptera: Culcidae) production sites in urban NicaraguaSamantha N Hammond
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720 7360, USA
J Med Entomol 44:851-60. 2007.... - Conformational changes in the solution structure of the dengue virus 5' end in the presence and absence of the 3' untranslated regionCharlotta Polacek
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, Berkeley, California 94720 7354, USA
J Virol 83:1161-6. 2009..We demonstrate that hybridization between the 5' and 3' CSs is independent of the UAR while the 5' UAR-3' UAR hybridization is dependent upon the 5' CS-3' CS interaction... - Recent advances in deciphering viral and host determinants of dengue virus replication and pathogenesisKaren Clyde
Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California, Berkeley, Berkeley, CA 94720-7360, USA
J Virol 80:11418-31. 2006 - Performance of an influenza rapid test in children in a primary healthcare setting in NicaraguaAubree Gordon
Division of Epidemiology, School of Public Health, University of California, Berkeley, California, United States of America
PLoS ONE 4:e7907. 2009..Influenza is major public health threat worldwide, yet the diagnostic accuracy of rapid tests in developing country settings is not well described... - Mycophenolic acid inhibits dengue virus infection by preventing replication of viral RNAMichael S Diamond
Division of Infectious Diseases, School of Public Health, University of California, Berkeley 94720-7360, USA
Virology 304:211-21. 2002..We conclude that MPA inhibits flavivirus infection by preventing synthesis and accumulation of viral RNA... - Single-step multiplex PCR assay for characterization of New World Leishmania complexesE Harris
Program in Molecular Pathogenesis, University of California, San Francisco, 94143 0422, USA
J Clin Microbiol 36:1989-95. 1998..This assay should be useful for rapid in-country identification of Leishmania parasites, particularly where different Leishmania complexes are found in the same geographical area... - Tropism of dengue virus in mice and humans defined by viral nonstructural protein 3-specific immunostainingScott J Balsitis
Division of Infectious Diseases, School of Public Health, and Department of Molecular and Cell Biology, University of California, Berkeley 94720 7354, California, USA
Am J Trop Med Hyg 80:416-24. 2009..Thus, NS3-specific immunostaining supports roles for infected phagocytes, hepatocytes, and, to a limited degree, endothelial cells in the pathogenesis of severe dengue... - Molecular biology of flavivirusesEva Harris
Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California, Berkeley, CA 94720 7360, USA
Novartis Found Symp 277:23-39; discussion 40, 71-3, 251-3. 2006..Although much remains to be elucidated about the molecular biology of flavivirus infection, progress is being made towards defining the cis and trans factors that regulate flavivirus translation and replication... - Interferon-dependent immunity is essential for resistance to primary dengue virus infection in mice, whereas T- and B-cell-dependent immunity are less criticalSujan Shresta
Division of Infectious Diseases, School of Public Health, University of California at Berkeley, Berkeley, California 94720, USA
J Virol 78:2701-10. 2004.... - S-phase-dependent enhancement of dengue virus 2 replication in mosquito cells, but not in human cellsAnna-Marija Helt
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, 140 Warren Hall, Berkeley, CA 94720-7360, USA
J Virol 79:13218-30. 2005..These results demonstrate a differential response of DEN2 to the mosquito and human cell cycle and provide a framework for detailed studies into the mechanisms mediating virus assembly... - Enhancement of dengue virus translation: role of the 3' untranslated region and the terminal 3' stem-loop domainKatherine L Holden
Division of Infectious Diseases, School of Public Health, University of California at Berkeley, Berkeley, CA 94720-7360, USA
Virology 329:119-33. 2004..Together, these results indicate that the DEN2 3'UTR, mediated in part by the 3'SL domain, enhances translation initiation, possibly after recognition of the 5' cap structure... - Sustainable transfer of biotechnology to developing countries: fighting poverty by bringing scientific tools to developing-country partnersJosefina Coloma
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, 1 Barker Hall 422, Berkeley, CA 94720 7354, USA
Ann N Y Acad Sci 1136:358-68. 2008..For 20 years, the Sustainable Sciences Institute has built scientific capacity in developing countries to recognize, prevent, and respond to the threats posed by disease... - Poly(A)-binding protein binds to the non-polyadenylated 3' untranslated region of dengue virus and modulates translation efficiencyCharlotta Polacek
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, 1 Barker Hall, Berkeley, CA 94720 7354, USA
J Gen Virol 90:687-92. 2009..Our findings show an expanded translation mechanism for PABP, binding to a viral RNA lacking a terminal poly(A) tail... - Early activation of natural killer and B cells in response to primary dengue virus infection in A/J miceSujan Shresta
Division of Infectious Diseases, School of Public Health, University of California at Berkeley, Berkeley, CA 94720-7360, USA
Virology 319:262-73. 2004..respectively, and (iii). splenocyte production of IFNgamma at day 14 p.i. We conclude that the early activities of NK cells, B cells and IgM, and later actions of IFNgamma and IgG likely play a role in the defense against DEN infection... - Antibodies play a greater role than immune cells in heterologous protection against secondary dengue virus infection in a mouse modelJennifer L Kyle
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, 1 Barker Hall 424, Berkeley, CA 94720 7354, USA
Virology 380:296-303. 2008..These findings are relevant for understanding both natural and vaccine-induced immunity to DENV... - Human enterovirus 109: a novel interspecies recombinant enterovirus isolated from a case of acute pediatric respiratory illness in NicaraguaNathan L Yozwiak
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, California, USA
J Virol 84:9047-58. 2010..Further experimentation is required to more fully characterize the pathogenic role, disease associations, and global distribution of EV109... - End-to-end communication in the modulation of translation by mammalian RNA virusesDianna Edgil
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720-7360, USA
Virus Res 119:43-51. 2006.... - Critical roles for both STAT1-dependent and STAT1-independent pathways in the control of primary dengue virus infection in miceSujan Shresta
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720, USA
J Immunol 175:3946-54. 2005..Collectively, these data begin to define the nature of the STAT1-dependent vs the STAT1-independent pathway in vivo... - Differences in dengue severity in infants, children, and adults in a 3-year hospital-based study in NicaraguaSamantha Nadia Hammond
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720-7360, USA
Am J Trop Med Hyg 73:1063-70. 2005..This represents one of the few comprehensive studies to analyze characteristics of dengue in infants, children, and adults in the same population and highlights age-related differences in dengue severity... - Lethal antibody enhancement of dengue disease in mice is prevented by Fc modificationScott J Balsitis
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California, United States of America
PLoS Pathog 6:e1000790. 2010..These observations provide insight into the pathogenesis of antibody-enhanced dengue disease and identify a novel strategy for the design of therapeutic antibodies against dengue... - From construction workers to architects: developing scientific research capacity in low-income countriesJosefina Coloma
Sustainable Sciences Institute, San Francisco, California, USA
PLoS Biol 7:e1000156. 2009..Solving global health challenges in a sustainable manner depends on explicitly addressing scientific capacity-building needs, as well as establishing long-term, meaningful partnerships with colleagues in the developing world... - Ultrasound measurement of gallbladder wall thickening as a diagnostic test and prognostic indicator for severe dengue in pediatric patientsJames A Colbert
Stanford University School of Medicine, Stanford, CA, USA
Pediatr Infect Dis J 26:850-2. 2007..We demonstrate that gallbladder wall thickening serves as a clinically relevant diagnostic test and prognostic indicator of severe dengue in pediatric populations... - High seroprevalence of antibodies against dengue virus in a prospective study of schoolchildren in Managua, NicaraguaAngel Balmaseda
, , Ministerio de Salud, Managua, Nicaragua
Trop Med Int Health 11:935-42. 2006..These and additional circulating serotypes were confirmed by plaque reduction neutralisation test. This study demonstrates surprisingly high transmission of DENV in urban Nicaragua... - Oscillating kissing stem-loop interactions mediate 5' scanning-dependent translation by a viral 3'-cap-independent translation elementAurelie M Rakotondrafara
Molecular, Cellular, and Developmental Biology Program and Plant Pathology Department, Iowa State University, Ames, Iowa 50011, USA
RNA 12:1893-906. 2006..We propose that the kissing interaction is repeatedly disrupted by the scanning ribosome and re-formed in an oscillating process that regulates ribosome entry on the RNA... - Quantitative assessment of the benefits of specific information technologies applied to clinical studies in developing countriesWilliam Avilés
Sustainable Sciences Institute, Managua, Nicaragua
Am J Trop Med Hyg 78:311-5. 2008..In addition to specific advantages, these information technologies benefited not only the study itself but numerous routine clinical and laboratory processes in the health center and laboratories of the Nicaraguan Ministry of Health... - Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strainFlavia Barreto dos Santos
Departamento de Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
Mem Inst Oswaldo Cruz 97:991-5. 2002..All amino acid changes differentiating strains related to the Asian/American-Asian genotype were observed in BR64022/98, indicating the Asiatic origin of the strain... - Emergence and global spread of a dengue serotype 3, subtype III virusWilliam B Messer
University of North Carolina, Chapel Hill, North Carolina, USA
Emerg Infect Dis 9:800-9. 2003..DENV-3, subtype III isolates from mild and severe disease outbreaks formed genetically distinct groups, which suggests a role for viral genetics in DHF... - Diagnosis of dengue virus infection by detection of specific immunoglobulin M (IgM) and IgA antibodies in serum and salivaAngel Balmaseda
National Center for Diagnosis and Reference, Ministry of Health, Granada, Nicaragua
Clin Diagn Lab Immunol 10:317-22. 2003.... - Molecular typing of dengue virus type 2 in BrazilMarize P Miagostovich
Laboratório de Flavivirus, Departmento de Virologia, Instituto Oswaldo Cruz FIOCRUZ, Av Brasil 4365, 21045 900 Rio de Janeiro, RJ, Brasil
Rev Inst Med Trop Sao Paulo 45:17-21. 2003..Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype)... - Phenotyping of peripheral blood mononuclear cells during acute dengue illness demonstrates infection and increased activation of monocytes in severe cases compared to classic dengue feverAnna P Durbin
Johns Hopkins Bloomberg School of Public Health, 624 North Broadway, Room 251, Baltimore, MD 21205, USA
Virology 376:429-35. 2008..Increased activation of monocytes and greater numbers of DEN-infected cells were associated with more severe dengue, implicating a role for monocyte activation in dengue immunopathogenesis... - Identification of Aedes albopictus in urban NicaraguaEmperatriz Del C Lugo
, , Ministerio de Salud, Managua, Nicaragua
J Am Mosq Control Assoc 21:325-7. 2005..The presence of Ae. albopictus in urban Nicaragua highlights the need for surveillance of areas thought to be free of the mosquito so that early detection and control activities can prevent its spread... - Serotype-specific differences in clinical manifestations of dengueAngel Balmaseda
, , Ministerio de Salud, Managua, Nicaragua
Am J Trop Med Hyg 74:449-56. 2006..86, CI 2.72-5.48) and more primary DENV infections with severe manifestations (OR 2.93, CI 2.00-4.28). These findings provide new data to characterize the pathogenic potential of distinct DENV serotypes in human populations... - The WHO dengue classification and case definitions: time for a reassessmentJacqueline L Deen
International Vaccine Institute, Kwanak PO Box 14, Seoul, Republic of Korea
Lancet 368:170-3. 2006 - A novel magnetic bead bioassay platform using a microchip-based sensor for infectious disease diagnosisTurgut Aytur
Department of Electrical Engineering and Computer Sciences, University of California at Berkeley, CA 94720-3200, USA
J Immunol Methods 314:21-9. 2006.... - Dendritic cell precursors are permissive to dengue virus and human immunodeficiency virus infectionWing-Hong Kwan
INSERM U255, , , Paris 6, , 75006 Paris, France
J Virol 79:7291-9. 2005..The results suggest that interstitial CD14(+) APC may contribute to HIV-1 and dengue virus infection and the shaping of an antiviral immune response... - Murine model for dengue virus-induced lethal disease with increased vascular permeabilitySujan Shresta
Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA
J Virol 80:10208-17. 2006..This report represents a significant advance in animal models for severe DEN disease, and it begins to provide mechanistic insights into DEN-induced disease in vivo... - Recombinant polypeptide antigen-based immunoglobulin G enzyme-linked immunosorbent assay for serodiagnosis of dengueFlavia B Dos Santos
Flavivirus Laboratory Department of Virology, Oswaldo Cruz Institute FIOCRUZ, Av Brasil, 4365, Manguinhos, Rio de Janeiro 21045 360, Brazil
Clin Vaccine Immunol 14:641-3. 2007..4% and a specificity of 93.3%. These easily produced antigens are a feasible, cost-effective alternative for generating reagents for dengue serological tests... - Innovative low cost technologies for biomedical research and diagnosis in developing countriesJosefina Coloma
Sustainable Sciences Institute, San Francisco, CA, USA
BMJ 329:1160-2. 2004 - Straight talk from... Eva HarrisEva Harris
Nat Med 13:1132. 2007 - Integration of information technologies in clinical studies in NicaraguaWilliam Avilés
Sustainable Sciences Institute, Managua, Nicaragua
PLoS Med 4:1578-83. 2007 - Analysis of recombinant dengue virus polypeptides for dengue diagnosis and evaluation of the humoral immune responseFlavia Barreto dos Santos
Departamento de Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
Am J Trop Med Hyg 71:144-52. 2004..Using this novel approach, we identified a candidate antigen to facilitate diagnosis of DENV infections and observed a surprising variability in antibody patterns in the clinical response to DENV infections... - Genetic analysis of Dengue 3 virus subtype III 5' and 3' non-coding regionsRicardo L A Silva
Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105, USA
Virus Res 135:320-5. 2008..The differences in the 3' NCRs reported here, as well as potential alterations in the structural and non-structural coding genes, may contribute to the increased pathogenicity of group B DENV-3... - Short report: assessment of the World Health Organization scheme for classification of dengue severity in NicaraguaAngel Balmaseda
, , Ministerio de Salud, Managua, Nicaragua
Am J Trop Med Hyg 73:1059-62. 2005..We found that strict application of the WHO criteria fails to detect a significant number of patients with severe manifestations of dengue, especially in adults...