BRIAN DAVID HARFE

Summary

Affiliation: University of Florida
Country: USA

Publications

  1. ncbi MicroRNAs in vertebrate development
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, PO Box 100266, Gainesville, FL 32610, USA
    Curr Opin Genet Dev 15:410-5. 2005
  2. doi Keeping up with the zone of polarizing activity: New roles for an old signaling center
    Brian D Harfe
    Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, Florida, USA
    Dev Dyn 240:915-9. 2011
  3. pmc The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 102:10898-903. 2005
  4. ncbi Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, 1600 SW Archer Road, Gainesville, FL 32610, USA
    Cell 118:517-28. 2004
  5. ncbi Identification of genes expressed in the mouse limb using a novel ZPA microarray approach
    Jason R Rock
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Cancer and Genetics Research Complex, 1376 Mowry Road, Gainesville, FL 32610, United States
    Gene Expr Patterns 8:19-26. 2007
  6. pmc Essential role for Dicer during skeletal muscle development
    Jason R O'Rourke
    Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32610 0266, USA
    Dev Biol 311:359-68. 2007
  7. doi In the limb AER Bmp2 and Bmp4 are required for dorsal-ventral patterning and interdigital cell death but not limb outgrowth
    Danielle M Maatouk
    Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32611, USA
    Dev Biol 327:516-23. 2009
  8. pmc Shh pathway activation is present and required within the vertebrate limb bud apical ectodermal ridge for normal autopod patterning
    Cortney M Bouldin
    Department of Molecular Genetics and Microbiology, The Genetics Institute, University of Florida, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 107:5489-94. 2010
  9. ncbi MicroRNAs in mammalian development and tumorigenesis
    Jason R O'Rourke
    Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32610, USA
    Birth Defects Res C Embryo Today 78:172-9. 2006
  10. pmc Identification of nucleus pulposus precursor cells and notochordal remnants in the mouse: implications for disk degeneration and chordoma formation
    Kyung Suk Choi
    Department of Molecular Genetics, University of Florida, College of Medicine, Gainesville, Florida 32610, USA
    Dev Dyn 237:3953-8. 2008

Collaborators

Detail Information

Publications14

  1. ncbi MicroRNAs in vertebrate development
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, PO Box 100266, Gainesville, FL 32610, USA
    Curr Opin Genet Dev 15:410-5. 2005
    ....
  2. doi Keeping up with the zone of polarizing activity: New roles for an old signaling center
    Brian D Harfe
    Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, Florida, USA
    Dev Dyn 240:915-9. 2011
    ..This review discusses the role and potential implications hedgehog signaling in the limb bud ectoderm plays in patterning the vertebrate limb...
  3. pmc The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 102:10898-903. 2005
    ..Thus, Dicer is required for the formation of normal mouse limbs. Strikingly, however, we did not detect defects in basic patterning or in tissue-specific differentiation of Dicer-deficient limb buds...
  4. ncbi Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, 1600 SW Archer Road, Gainesville, FL 32610, USA
    Cell 118:517-28. 2004
    ..Genetic studies of the effects of limiting accessibility of Shh within the limb support this model, in which the effect of the Shh morphogen is dictated by a temporal as well as a spatial gradient...
  5. ncbi Identification of genes expressed in the mouse limb using a novel ZPA microarray approach
    Jason R Rock
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Cancer and Genetics Research Complex, 1376 Mowry Road, Gainesville, FL 32610, United States
    Gene Expr Patterns 8:19-26. 2007
    ..The expression of two other genes was confirmed by rtPCR. The methods described in this report will be applicable for identifying genes enriched in Shh-expressing cells throughout development...
  6. pmc Essential role for Dicer during skeletal muscle development
    Jason R O'Rourke
    Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32610 0266, USA
    Dev Biol 311:359-68. 2007
    ..These observations demonstrate key roles for Dicer in skeletal muscle and implicate miRNAs as critical components required for embryonic myogenesis...
  7. doi In the limb AER Bmp2 and Bmp4 are required for dorsal-ventral patterning and interdigital cell death but not limb outgrowth
    Danielle M Maatouk
    Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32611, USA
    Dev Biol 327:516-23. 2009
    ..Our data suggests that AER expression of Bmp2 and Bmp4 is required for digit and dorsal-ventral patterning but surprisingly not for limb outgrowth...
  8. pmc Shh pathway activation is present and required within the vertebrate limb bud apical ectodermal ridge for normal autopod patterning
    Cortney M Bouldin
    Department of Molecular Genetics and Microbiology, The Genetics Institute, University of Florida, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 107:5489-94. 2010
    ..These data indicate that contrary to previous accounts, the Hh signaling pathway is present and required in the developing limb AER for normal autopod development...
  9. ncbi MicroRNAs in mammalian development and tumorigenesis
    Jason R O'Rourke
    Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32610, USA
    Birth Defects Res C Embryo Today 78:172-9. 2006
    ..This review is designed to highlight recent advances in the study of miRNAs with a particular emphasis on their roles in mammalian development and cancer progression...
  10. pmc Identification of nucleus pulposus precursor cells and notochordal remnants in the mouse: implications for disk degeneration and chordoma formation
    Kyung Suk Choi
    Department of Molecular Genetics, University of Florida, College of Medicine, Gainesville, Florida 32610, USA
    Dev Dyn 237:3953-8. 2008
    ..In addition, the identification of notochordal remnants in mice is the first step towards generating an in vivo model of chordoma...
  11. doi Aberrant FGF signaling, independent of ectopic hedgehog signaling, initiates preaxial polydactyly in Dorking chickens
    Cortney M Bouldin
    Department of Molecular Genetics and Microbiology, The Genetics Institute, University of Florida, Gainesville, FL 32610, USA
    Dev Biol 334:133-41. 2009
    ..Our data indicates that in Dorking chickens, preaxial polydactyly is initiated independent of Shh...
  12. pmc Cell lineage analysis demonstrates an endodermal origin of the distal urethra and perineum
    Ashley W Seifert
    Department of Zoology, University of Florida, Cancer Genetics Research Complex, PO Box 103610, Gainesville, FL 32610 3610, USA
    Dev Biol 318:143-52. 2008
    ....
  13. pmc Multiphasic and tissue-specific roles of sonic hedgehog in cloacal septation and external genitalia development
    Ashley W Seifert
    Department of Biology, University of Florida, Cancer Genetics Research Complex, PO Box 103610, Gainesville, FL 32610 3610, USA
    Development 136:3949-57. 2009
    ....
  14. doi Expression of TMEM16 paralogs during murine embryogenesis
    Jason R Rock
    Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA
    Dev Dyn 237:2566-74. 2008
    ..These data should encourage investigations into the functions of TMEM16 paralogs in vertebrate development...

Research Grants9

  1. Identify and characterize novel Wnt pathway genes
    Brian Harfe; Fiscal Year: 2006
    ..The PIs have experience working with C. elegans, mice and chick model systems and are positioned to take full advantage of each system. ..
  2. Age-related changes in the intervertebral disk.
    Brian Harfe; Fiscal Year: 2007
    ....
  3. Formation of the intervertebral disk: a molecular approach
    Brian Harfe; Fiscal Year: 2009
    ....
  4. Formation of the intervertebral disk: a molecular approach
    Brian Harfe; Fiscal Year: 2009
    ..Their ability to survive, proliferates, and form all nucleus pulposus cell types will be determined using molecular markers. ..
  5. Formation of the intervertebral disk: a molecular approach
    BRIAN DAVID HARFE; Fiscal Year: 2010
    ....
  6. Age-related changes in the intervertebral disk.
    BRIAN DAVID HARFE; Fiscal Year: 2010
    ....