P E Hardin

Summary

Affiliation: University of Houston
Country: USA

Publications

  1. pmc Drosophila CLOCK is constitutively expressed in circadian oscillator and non-oscillator cells
    Jerry H Houl
    Department of Biology and Biochemistry, University of Houston, Houston, TX, USA
    J Biol Rhythms 21:93-103. 2006
  2. pmc PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms
    Juliana Benito
    Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204, USA
    J Neurosci 27:2539-47. 2007
  3. ncbi request reprint Transcription regulation within the circadian clock: the E-box and beyond
    Paul E Hardin
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 5001, USA
    J Biol Rhythms 19:348-60. 2004
  4. ncbi request reprint Activating inhibitors and inhibiting activators: a day in the life of a fly
    P E Hardin
    Department of Biology and Biochemistry, University of Houston, Texas 77204 5513, USA
    Curr Opin Neurobiol 8:642-7. 1998
  5. pmc From biological clock to biological rhythms
    P E Hardin
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 5513, USA
    Genome Biol 1:REVIEWS1023. 2000
  6. ncbi request reprint The circadian timekeeping system of Drosophila
    Paul E Hardin
    Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Road, Houston, Texas 77204 5001, USA
    Curr Biol 15:R714-22. 2005
  7. ncbi request reprint Perspectives: neurobiology. The CRYs fo flies and mice
    P E Hardin
    Department of Biology, University of Houston, Houston, TX 77204, USA
    Science 286:2460-1. 1999
  8. ncbi request reprint A new role for cryptochrome in a Drosophila circadian oscillator
    B Krishnan
    Department of Biology and Biochemistry and Biological Clocks Program, University of Houston, Houston, Texas 77204, USA
    Nature 411:313-7. 2001
  9. ncbi request reprint Interlocked feedback loops within the Drosophila circadian oscillator
    N R Glossop
    Department of Biology and Biochemistry and Biological Clocks Program, University of Houston, Houston, TX 77204 5513, USA
    Science 286:766-8. 1999
  10. ncbi request reprint Disruption of synaptic transmission or clock-gene-product oscillations in circadian pacemaker cells of Drosophila cause abnormal behavioral rhythms
    M Kaneko
    Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    J Neurobiol 43:207-33. 2000

Collaborators

Detail Information

Publications46

  1. pmc Drosophila CLOCK is constitutively expressed in circadian oscillator and non-oscillator cells
    Jerry H Houl
    Department of Biology and Biochemistry, University of Houston, Houston, TX, USA
    J Biol Rhythms 21:93-103. 2006
    ....
  2. pmc PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms
    Juliana Benito
    Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204, USA
    J Neurosci 27:2539-47. 2007
    ..PDP1epsilon is nevertheless required for behavioral rhythmicity, which suggests that it functions to regulate oscillator output...
  3. ncbi request reprint Transcription regulation within the circadian clock: the E-box and beyond
    Paul E Hardin
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 5001, USA
    J Biol Rhythms 19:348-60. 2004
    ....
  4. ncbi request reprint Activating inhibitors and inhibiting activators: a day in the life of a fly
    P E Hardin
    Department of Biology and Biochemistry, University of Houston, Texas 77204 5513, USA
    Curr Opin Neurobiol 8:642-7. 1998
    ..These studies, along with those in other organisms, suggest that circadian feedback loops are widespread and that genes within these feedback loops are conserved between Drosophila and mammals...
  5. pmc From biological clock to biological rhythms
    P E Hardin
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 5513, USA
    Genome Biol 1:REVIEWS1023. 2000
    ..Completion of whole genome sequences and the use of differential gene expression technology will hasten the discovery of the clock output pathways that control diverse rhythmic phenomena...
  6. ncbi request reprint The circadian timekeeping system of Drosophila
    Paul E Hardin
    Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Road, Houston, Texas 77204 5001, USA
    Curr Biol 15:R714-22. 2005
    ..I will also discuss where work remains to be done to give a comprehensive picture of the circadian clock in Drosophila and likely many other organisms...
  7. ncbi request reprint Perspectives: neurobiology. The CRYs fo flies and mice
    P E Hardin
    Department of Biology, University of Houston, Houston, TX 77204, USA
    Science 286:2460-1. 1999
  8. ncbi request reprint A new role for cryptochrome in a Drosophila circadian oscillator
    B Krishnan
    Department of Biology and Biochemistry and Biological Clocks Program, University of Houston, Houston, Texas 77204, USA
    Nature 411:313-7. 2001
    ..These results demonstrate a photoreceptor-independent role for CRY in the periphery and imply fundamental differences between central and peripheral oscillator mechanisms in Drosophila...
  9. ncbi request reprint Interlocked feedback loops within the Drosophila circadian oscillator
    N R Glossop
    Department of Biology and Biochemistry and Biological Clocks Program, University of Houston, Houston, TX 77204 5513, USA
    Science 286:766-8. 1999
    ....
  10. ncbi request reprint Disruption of synaptic transmission or clock-gene-product oscillations in circadian pacemaker cells of Drosophila cause abnormal behavioral rhythms
    M Kaneko
    Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    J Neurobiol 43:207-33. 2000
    ..Consistent with this molecular defect, the proportion of rhythmic individuals in constant darkness was subnormal in flies expressing PER under the control of tim-gal4, and their behavior in light:dark cycles was abnormal...
  11. ncbi request reprint Specific sequences outside the E-box are required for proper per expression and behavioral rescue
    L C Lyons
    Department of Biology and Biochemistry, University of Houston, TX, USA
    J Biol Rhythms 15:472-82. 2000
    ..These results indicate that the dCLK-CYC target site extends beyond the E-box and that factors other than dCLK and CYC modulate per transcription...
  12. pmc Simulation of Drosophila circadian oscillations, mutations, and light responses by a model with VRI, PDP-1, and CLK
    Paul Smolen
    Department of Neurobiology and Anatomy, W M Keck Center for the Neurobiology of Learning and Memory, The University of Texas Houston Medical School, Houston, Texas 77225, USA
    Biophys J 86:2786-802. 2004
    ..Temperature compensation of oscillation period could be simulated if temperature elevation slowed PER nuclear entry or PER phosphorylation. The model makes experimental predictions, some of which could be tested in transgenic Drosophila...
  13. ncbi request reprint Drosophila photoreceptors contain an autonomous circadian oscillator that can function without period mRNA cycling
    Y Cheng
    Department of Biology, Texas A and M University, College Station, Texas 77843, USA
    J Neurosci 18:741-50. 1998
    ..These results show that per mRNA cycling is not required for PER cycling and indicate that Drosophila photoreceptors R1-R6 contain a tissue autonomous circadian oscillator...
  14. pmc PER-dependent rhythms in CLK phosphorylation and E-box binding regulate circadian transcription
    Wangjie Yu
    Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204 5001, USA
    Genes Dev 20:723-33. 2006
    ....
  15. pmc Rhythmic E-box binding by CLK-CYC controls daily cycles in per and tim transcription and chromatin modifications
    Pete Taylor
    Center for Research on Biological Clocks, Department of Biology, Texas A and M University, College Station, Texas 77845 3258, USA
    Mol Cell Biol 28:4642-52. 2008
    ....
  16. ncbi request reprint vrille, Pdp1, and dClock form a second feedback loop in the Drosophila circadian clock
    Shawn A Cyran
    Department of Biology, New York University, 100 Washington Square East, New York, NY 10003, USA
    Cell 112:329-41. 2003
    ..Thus, VRI and PDP1, together with dClock itself, comprise a second feedback loop in the Drosophila clock that gives rhythmic expression of dClock, and probably of other genes, to generate accurate circadian rhythms...
  17. ncbi request reprint Circadian clocks in antennal neurons are necessary and sufficient for olfaction rhythms in Drosophila
    Shintaro Tanoue
    Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Road, Houston, TX 77204 USA
    Curr Biol 14:638-49. 2004
    ..We previously showed that peripheral oscillators are required to mediate rhythmic olfactory responses in the antenna, but the identity and relative autonomy of these peripheral oscillators has not been defined...
  18. pmc Transcriptional feedback loop regulation, function, and ontogeny in Drosophila
    J Benito
    Department of Biology, Texas A and M University, College Station, Texas 77843 3258, USA
    Cold Spring Harb Symp Quant Biol 72:437-44. 2007
    ..Substantial insight into each of these issues has been gained from experiments performed in our lab and others and is summarized here...
  19. pmc Two alternatively spliced transcripts from the Drosophila period gene rescue rhythms having different molecular and behavioral characteristics
    Y Cheng
    Department of Biology, Texas A and M University, College Station, Texas 77843, USA
    Mol Cell Biol 18:6505-14. 1998
    ..4 h, respectively. Although no appreciable differences in type A and type B' mRNA cycling were observed, a slower accumulation of PER in flies making only type A transcripts suggests that the intron affects the translation of per mRNA...
  20. ncbi request reprint Central and peripheral circadian oscillator mechanisms in flies and mammals
    Nicholas R J Glossop
    Department of Biology and Biochemistry, University of Houston, 369 Science and Research 2 Bldg, Houston, TX 77204 5001, USA
    J Cell Sci 115:3369-77. 2002
    ..The core feedback loop in peripheral oscillators might therefore be functionally well conserved, and central oscillators could be specialized versions of a basic oscillator design...
  21. pmc DOUBLETIME plays a noncatalytic role to mediate CLOCK phosphorylation and repress CLOCK-dependent transcription within the Drosophila circadian clock
    Wangjie Yu
    Department of Biology, Texas A and M University, College Station, TX 77843, USA
    Mol Cell Biol 29:1452-8. 2009
    ..A similar mechanism likely operates in mammals, given the conserved activities of PER, DBT, and CLK orthologs...
  22. pmc Survey of transcripts in the adult Drosophila brain
    K L Posey
    Department of Biology and Biochemistry, Institute of Molecular Biology, University of Houston, Houston, TX 77204-5513, USA
    Genome Biol 2:RESEARCH0008. 2001
    ..This library should be a useful resource that will help in determining how basic brain functions operate at the molecular level...
  23. pmc A circadian enhancer mediates PER-dependent mRNA cycling in Drosophila melanogaster
    H Hao
    Department of Biology, Texas A and M University, College Station 77843 3258, USA
    Mol Cell Biol 17:3687-93. 1997
    ..An E-box sequence within this 69-bp fragment is necessary for high-level expression, but not for rhythmic expression, indicating that PER mediates circadian transcription through other sequences in this fragment...
  24. ncbi request reprint VRILLE feeds back to control circadian transcription of Clock in the Drosophila circadian oscillator
    Nicholas R J Glossop
    Department of Biology and Biochemistry, University of Houston, 369 Science and Research Bldg 2, Houston, TX 77204, USA
    Neuron 37:249-61. 2003
    ..The blue light photoreceptor encoding cryptochrome (cry) gene is also a target for VRI repression, suggesting a broader role for VRI in the rhythmic repression of output genes that cycle in phase with Clk...
  25. pmc CLOCK expression identifies developing circadian oscillator neurons in the brains of Drosophila embryos
    Jerry H Houl
    Center for Research on Biological Clocks, Department of Biology, Texas A and M University, 3258 TAMU, College Station, TX 77843, USA
    BMC Neurosci 9:119. 2008
    ..Since CLK is required to initiate feedback loop function, defining the pattern of CLK expression in embryos and larvae will shed light on oscillator neuron development...
  26. ncbi request reprint Circadian rhythms in olfactory responses of Drosophila melanogaster
    B Krishnan
    Department of Biology and Biochemistry and Biological Clocks Program, University of Houston, Texas 77204 5513, USA
    Nature 400:375-8. 1999
    ....
  27. ncbi request reprint Essential and expendable features of the circadian timekeeping mechanism
    Paul E Hardin
    Department of Biology, Center for Research on Biological Clocks, Texas A and M University, College Station, TX 77843 3258, USA
    Curr Opin Neurobiol 16:686-92. 2006
    ....
  28. ncbi request reprint Central and peripheral circadian oscillators in Drosophila
    Paul E Hardin
    Department of Biology and Biochemistry, University of Houston, Houston, TX 771204 5001, USA
    Novartis Found Symp 253:140-50; discussion 150-60. 2003
    ....
  29. pmc G protein-coupled receptor kinase 2 is required for rhythmic olfactory responses in Drosophila
    Shintaro Tanoue
    Department of Biology, Texas A and M University, College Station, Texas 77843 3258, USA
    Curr Biol 18:787-94. 2008
    ..Because responses to odors are initiated by heteromeric OR complexes that form odor-gated and cyclic-nucleotide-activated cation channels, we tested whether regulators of ORs were under circadian-clock control...
  30. ncbi request reprint The intrinsic circadian clock within the cardiomyocyte
    David J Durgan
    Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Ctr at Houston, 2121 W Holcombe Blvd, IBT 1011, Houston, TX 77030, USA
    Am J Physiol Heart Circ Physiol 289:H1530-41. 2005
    ..e., 2.5% serum). Furthermore, our data suggest that norepinephrine, unlike glucose, influences the timing of the circadian clock within the heart and that the circadian clock may be a novel mechanism regulating myocardial metabolism...
  31. pmc Spike amplitude of single-unit responses in antennal sensillae is controlled by the Drosophila circadian clock
    Parthasarathy Krishnan
    Department of Biology, Texas A and M University, College Station, Texas 77843 3258, USA
    Curr Biol 18:803-7. 2008
    ..These results argue that rhythms in GPRK2 levels control OR localization and OR-dependent ion channel activity and/or composition to mediate rhythms in spontaneous spike amplitude...
  32. pmc The circadian output gene takeout is regulated by Pdp1epsilon
    Juliana Benito
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 5001, USA
    Proc Natl Acad Sci U S A 107:2544-9. 2010
    ..Furthermore, flies with reduced or elevated Pdp1epsilon levels in the fat body display courtship defects, identifying Pdp1epsilon as an important transcriptional regulator in that tissue...
  33. pmc The blue-light photoreceptor CRYPTOCHROME is expressed in a subset of circadian oscillator neurons in the Drosophila CNS
    Juliana Benito
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 5001, USA
    J Biol Rhythms 23:296-307. 2008
    ..Oscillators in DN2s and DN3s entrain to environmental light-dark cycles, which implies that they are entrained indirectly by retinal photoreceptors, extraretinal photoreceptors, or other CRY-expressing cells...
  34. pmc A Drosophila nonvisual arrestin is required for the maintenance of olfactory sensitivity
    Hong Ge
    Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Road, Houston, TX 77204, USA
    Chem Senses 31:49-62. 2006
    ..The reduced receptor potentials in krz(1) antenna indicate that nonvisual arrestins are required for the early odor-induced signaling events within the ORNs...
  35. pmc Circadian rhythms from multiple oscillators: lessons from diverse organisms
    Deborah Bell-Pedersen
    Center for Research on Biological Clocks, Department of Biology, Texas A and M University, College Station, Texas 77843 3258, USA
    Nat Rev Genet 6:544-56. 2005
    ..In both cases, the temporal coordination of a multi-oscillator system is essential for producing robust circadian rhythms of gene expression and biological activity...
  36. ncbi request reprint The circadian clock within the cardiomyocyte is essential for responsiveness of the heart to fatty acids
    David J Durgan
    United States Department of Agriculture Agricultural Research Service Children s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 281:24254-69. 2006
    ..These studies expose a causal relationship between the circadian clock within the cardiomyocyte with responsiveness of the heart to fatty acids and myocardial triglyceride metabolism...
  37. pmc A role for the adult fat body in Drosophila male courtship behavior
    Anna A Lazareva
    Department of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America
    PLoS Genet 3:e16. 2007
    ..In support of this idea, we demonstrate that Takeout, a protein known to influence mating, is present in the hemolymph of adult males but not females and acts as a secreted protein...
  38. ncbi request reprint Disruption of the circadian clock within the cardiomyocyte influences myocardial contractile function, metabolism, and gene expression
    Molly S Bray
    US Department of Agriculture, Agricultural Research Service Children s Nutrition Research Center, Department of Pediatrics, Houston, TX 77030, USA
    Am J Physiol Heart Circ Physiol 294:H1036-47. 2008
    ..These studies suggest that the cardiomyocyte circadian clock influences myocardial contractile function, metabolism, and gene expression...
  39. pmc Spatial and circadian regulation of cry in Drosophila
    - Hao Zheng
    Department of Biology and Center for Research on Biological Clocks, Texas A and M University, College Station, TX 77843 3258, USA
    J Biol Rhythms 23:283-95. 2008
    ....
  40. ncbi request reprint Drosophila CLOCK protein is under posttranscriptional control and influences light-induced activity
    Eun Young Kim
    Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, NJ 08854, USA
    Neuron 34:69-81. 2002
    ..Our findings suggest that, in addition to a role in generating circadian rhythms, dCLK modulates the direct effects of light on locomotion...
  41. ncbi request reprint Circadian transcription: passing the HAT to CLOCK
    Paul E Hardin
    Department of Biology and Center for Research on Biological Clocks, Texas A and M University, College Station, TX 77843, USA
    Cell 125:424-6. 2006
    ..2006) reveal that rhythms in histone acetylation are necessary for rhythmic transcription and that the histone acetyl transferase responsible is CLOCK, a key transcription factor that is essential for circadian oscillator function...
  42. pmc A functional genomics strategy reveals clockwork orange as a transcriptional regulator in the Drosophila circadian clock
    Akira Matsumoto
    Laboratory for Systems Biology, Center for Developmental Biology, RIKEN, Kobe, Hyogo, Japan
    Genes Dev 21:1687-700. 2007
    ....
  43. pmc A DOUBLETIME kinase binding domain on the Drosophila PERIOD protein is essential for its hyperphosphorylation, transcriptional repression, and circadian clock function
    Eun Young Kim
    Rutgers University, Center for Advanced Biotechnology and Medicine, Piscataway, NJ 08854, USA
    Mol Cell Biol 27:5014-28. 2007
    ....
  44. ncbi request reprint Use of firefly luciferase activity assays to monitor circadian molecular rhythms in vivo and in vitro
    Wangjie Yu
    Department of Biology and Center for Research on Biological Rhythms, Texas A and M University, College Station, USA
    Methods Mol Biol 362:465-80. 2007
    ..Here we will describe how luciferase assays are performed and analyzed to measure gene expression rhythms in Drosophila...
  45. ncbi request reprint Circadian oscillators of Drosophila and mammals
    Wangjie Yu
    Department of Biology and Center for Research on Biological Clocks, Texas A and M University, College Station, TX 77843 3258, USA
    J Cell Sci 119:4793-5. 2006
  46. pmc Rapid attenuation of circadian clock gene oscillations in the rat heart following ischemia-reperfusion
    Theodore A Kung
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106, USA
    J Mol Cell Cardiol 43:744-53. 2007
    ..In conclusion, these data show that in a rodent model of myocardial I/R, circadian clocks within the ischemic region become rapidly impaired, through a mechanism that appears to be independent of hypoxia...