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Genomes and Genes | Manami HaraSummaryAffiliation: University of Chicago Country: USA Publications
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Publications
A mouse model for studying intrahepatic islet transplantationManami Hara
Department of Medicine, University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637, USA
Transplantation 78:615-8. 2004..Initial events 24 hr after islet transplantation in the liver include beta-cell loss and hepatic ischemic injuries...
Imaging pancreatic beta-cells in the intact pancreasManami Hara
Dept of Medicine, University of Chicago, 5841 South Maryland Ave, MC1027, Chicago, IL 60637, USA
Am J Physiol Endocrinol Metab 290:E1041-7. 2006....- High resolution optical imaging of infarction in intact organsManami Hara
Department of Medicine, The University of Chicago, Chicago, IL 60637, USA
Biotechniques 39:373-6. 2005.... - Transgenic mice with green fluorescent protein-labeled pancreatic beta -cellsManami Hara
Department of Medicine, The University of Chicago, Illinois 60637, USA
Am J Physiol Endocrinol Metab 284:E177-83. 2003..4 +/- 0.2% (n = 6) of the cells in the pancreas of newborn (P1) and 0.9 +/- 0.1% (n = 5) of 8-wk-old mice were beta-cells. The MIP-GFP-transgenic mouse may be a useful tool for studying beta-cell biology in normal and diabetic animals... - Identification of three missense mutations in the peroxisome proliferator-activated receptor alpha gene in Japanese subjects with maturity-onset diabetes of the youngM Hara
Howard Hughes Medical Institute, The University of Chicago, IL 60637, USA
J Hum Genet 46:285-8. 2001..Variation in the coding region of PPARA is unlikely to be a major cause of MODY in Japanese people... - Sustained expression of hepatocyte nuclear factor-6 leads to loss of pancreatic beta-cells by apoptosisM Hara
Department of Medicine, University of Chicago, Chicago, Illinois 60637, United States
Exp Clin Endocrinol Diabetes 115:654-61. 2007..This severe loss of beta-cells involved programmed cell death... - Recovery of islet beta-cell function in streptozotocin- induced diabetic mice: an indirect role for the spleenDengping Yin
Section of Transplantation, Department of Surgery, The University of Chicago, Chicago, IL 60637, USA
Diabetes 55:3256-63. 2006..This study supports the possibility of restoring beta-cell function in diabetic individuals and points to a role for the spleen in facilitating this process... - Relationship of calpain-10 genotype to phenotypic features of polycystic ovary syndromeDavid A Ehrmann
Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
J Clin Endocrinol Metab 87:1669-73. 2002..In addition to its association with insulin levels in African-Americans, the 112/121-haplotype combination was associated with an approximate 2-fold increase in risk of PCOS in both African-Americans and whites... - Association of the (AU)AT-rich element polymorphism in PPP1R3 with hormonal and metabolic features of polycystic ovary syndromeSergio Y Alcoser
Department of Biochemistry, The University of Chicago, Chicago, Illinois 60637, USA
J Clin Endocrinol Metab 89:2973-6. 2004..8 pmol/liter). Studies of diabetic and nondiabetic PCOS women of other racial and ethnic backgrounds will be necessary to assess the impact of this and other variants in PPP1R3 upon the phenotype and natural history of women with PCOS... - Reversal of diabetes in non-obese diabetic mice without spleen cell-derived beta cell regenerationAnita S Chong
Section of Transplantation, Department of Surgery, University of Chicago, Chicago, IL 60637, USA
Science 311:1774-5. 2006..Using the same approach, we report here that this treatment cured 32% of NOD mice of established diabetes (>340 milligrams per deciliter blood glucose), although beta cells in these mice were not derived from donor splenocytes... - Islet architecture: A comparative studyAbraham Kim
Department of Medicine, The University of Chicago, Chicago, IL, USA
Islets 1:129-36. 2009..In summary, human and mouse islets share common architectural features that may reflect demand for insulin. Comparative studies of islet architecture may lead to a better understanding of islet development and function... - Islet formation during the neonatal development in miceKevin Miller
Department of Medicine, The University of Chicago, Chicago, IL, USA
PLoS ONE 4:e7739. 2009..Mathematical modeling of the fission process in the neonatal islet formation is also presented... - Quantification of pancreatic islet distribution in situ in miceGerman Kilimnik
Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
Am J Physiol Endocrinol Metab 297:E1331-8. 2009.... - Altered islet composition and disproportionate loss of large islets in patients with type 2 diabetesGerman Kilimnik
Department of Medicine, The University of Chicago, Chicago, Illinois, United States of America
PLoS ONE 6:e27445. 2011.... - Intraislet production of GLP-1 by activation of prohormone convertase 1/3 in pancreatic α-cells in mouse models of ß-cell regenerationGerman Kilimnik
Department of Medicine, The University of Chicago, Chicago, IL USA
Islets 2:149-55. 2010..Our study suggests an important role of α-cells for ß-cell proliferation and further for the endocrine cell network within an islet... - GSK-3 inactivation or depletion promotes β-cell replication via down regulation of the CDK inhibitor, p27 (Kip1)Jeffrey Stein
Section of Adult and Pediatric Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Chicago, Chicago, IL, USA
Islets 3:21-34. 2011..Altogether our findings indicate that p27 levels in β-cells are stabilized by GSK-3 and thus p27 down regulation following GSK-3 depletion / inactivation plays a critical role in promoting β-cell replication... - Insulin resistance is attenuated in women with polycystic ovary syndrome with the Pro(12)Ala polymorphism in the PPARgamma geneManami Hara
Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
J Clin Endocrinol Metab 87:772-5. 2002..03) and 2 h (688 +/- 103 vs. 10190 +/- 99 pmol/liter; P = 0.04) time points during the oral glucose tolerance test. We conclude that Pro(12)Ala in PPARG is a modifier of insulin resistance in Caucasian women with PCOS... - Imaging endoplasmic reticulum calcium with a fluorescent biosensor in transgenic miceManami Hara
Department of Medicine MC1027, The University of Chicago, 5841 South Maryland Ave, Chicago, IL 60637, USA
Am J Physiol Cell Physiol 287:C932-8. 2004.... - Quantitative analysis of pancreatic polypeptide cell distribution in the human pancreasXiaojun Wang
Department of Surgery, The University of Chicago, Chicago, Illinois, United States of America
PLoS ONE 8:e55501. 2013..This study presents an important implication for the regional selection and interpretation of the results... - Quantification of islet size and architectureGerman Kilimnik
Department of Medicine, The University of Chicago, Chicago, IL, USA
Islets 4:167-72. 2012..In this article, we describe the method of image capture, parameters measured, data analysis and interpretation of the data... - Extrapancreatic insulin-producing cells in multiple organs in diabetesHideto Kojima
Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 101:2458-63. 2004..These observations have implications for the regulation of insulin gene expression, modulation of self-tolerance by insulin gene expression, and strategies for the generation of insulin-producing cells for the treatment of diabetes... - Role of HNF-1alpha in regulating the expression of genes involved in cellular growth and proliferation in pancreatic beta-cellsYuji Uchizono
Department of Medicine, The University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637, USA
Diabetes Res Clin Pract 84:19-26. 2009..These results provide insights into the mechanisms whereby HNF-1alpha affects beta-cell function... - Generation of embryonic stem cells from mouse insulin I promoter-green fluorescent protein transgenic mice and characterization in a teratoma modelWieslawa M Milewski
Department of Medicine, The University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637, USA
In Vitro Cell Dev Biol Anim 45:1-5. 2009..The MIP-GFP ESCs should be a useful research tool to study the differentiation capacity of ESCs toward pancreatic lineages... - No mantle formation in rodent islets -- the prototype of islet revisitedMichael Kharouta
Department of Medicine, The University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637, United States
Diabetes Res Clin Pract 85:252-7. 2009..In summary, human and mouse islets share common architectural features as endocrine micro-organs. Since these may hold a key to better understanding islet plasticity, our concept of the prototypic islet should be revised... - Pancreatic islet plasticity: interspecies comparison of islet architecture and compositionDonald J Steiner
Department of Medicine, The University of Chicago, Chicago, IL USA
Islets 2:135-45. 2010..We propose that this plasticity reflects evolutionary acquired adaptation induced by altered physiological conditions, rather than inherent disparities between species... 
Coating human pancreatic islets with CD4(+)CD25(high)CD127(-) regulatory T cells as a novel approach for the local immunoprotectionNatalia Marek
Department of Surgery, Section of Transplantation, University of Chicago, IL 60637, USA
Ann Surg 254:512-8; discussion 518-9. 2011..To develop a novel approach for local immunoprotection using CD4(+)CD25(high)CD127(-) T regulatory cells (Tregs) attached to the surface of the islets before transplantation...- Beta-cell transcription factors and diabetes: mutations in the coding region of the BETA2/NeuroD1 (NEUROD1) and Nkx2.2 (NKX2B) genes are not associated with maturity-onset diabetes of the young in JapaneseH Furuta
Department of Biochemistry and Molecular Biology, The University of Chicago, Illinois 60637, USA
Diabetes 47:1356-8. 1998 - Familial juvenile autoimmune hypothyroidism, pituitary enlargement, obesity, and insulin resistanceSirimon Reutrakul
The J.P. Kennedy Jr. Mental Retardation Research Center, University of Chicago, Illinois 60637, USA
Thyroid 14:311-9. 2004..In conclusion, the remarkably high incidence of childhood autoimmune hypothyroidism, pituitary enlargement, insulin resistance and obesity in this family is not linked to known HLA types or known gene defects... - Liver ischemia contributes to early islet failure following intraportal transplantation: benefits of liver ischemic-preconditioningD Yin
Section of Transplantation, Department of Surgery, The University of Chicago, Chicago, Illinois, USA
Am J Transplant 6:60-8. 2006..These data suggest that islet embolism in the portal vein is a major cause of functional loss following IPIT that can be prevented by liver IP... - Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitusY Horikawa
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA
Nat Genet 26:163-75. 2000..This finding suggests a novel pathway that may contribute to the development of type 2 diabetes... - Cloning of rat and mouse P2Y purinoceptorsY Tokuyama
Department of Medicine, Howard Hughes Medical Institute, University of Chicago, Illinois 60637, USA
Biochem Biophys Res Commun 211:211-8. 1995..The cloned rat P2Y purinoceptor was expressed in Xenopus laevis oocytes and possessed the properties expected for this receptor subtype... - Glial cell line-derived neurotrophic factor increases beta-cell mass and improves glucose toleranceSimon Mwangi
Division of Digestive Diseases, Emory University, Atlanta, Georgia, USA
Gastroenterology 134:727-37. 2008..Glial cell line-derived neurotrophic factor (GDNF) is a growth factor that plays a role in the development and survival of the enteric nervous system. We investigated the role of GDNF in regulating beta-cell survival... - Insulin regulates islet alpha-cell function by reducing KATP channel sensitivity to adenosine 5'-triphosphate inhibitionYuk M Leung
Departments of Medicine and Physiology, University of Toronto, Canada
Endocrinology 147:2155-62. 2006..This may be an underlying basis for the proposed sequential glucose-insulin regulation of alpha-cell glucagon secretion, which becomes distorted in diabetes, leading to dysregulated glucagon secretion... - The fusion of bone-marrow-derived proinsulin-expressing cells with nerve cells underlies diabetic neuropathyTomoya Terashima
Department of Molecular Genetics in Medicine, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan
Proc Natl Acad Sci U S A 102:12525-30. 2005..These results suggest that bone-marrow-derived cells may play an important role in the pathogenesis of diabetic complications... - Electrophysiological characterization of pancreatic islet cells in the mouse insulin promoter-green fluorescent protein mouseYuk M Leung
Department of Medicine, Room 7308, or Dr Herbert Y Gaisano, Room 7226, Medical Sciences Building, 1 King s College Circle, University of Toronto, Toronto, Ontario M5S 1A8, Canada
Endocrinology 146:4766-75. 2005..The MIP-GFP mouse islet is therefore a reliable model to efficiently examine alpha-cell and beta-cell physiology and should greatly facilitate examination of their pathophysiology when the MIP-GFP mice are crossed with diabetic models... - Imaging Beta cell development in real-time using pancreatic explants from mice with green fluorescent protein-labeled pancreatic Beta cellsSubhadra C Gunawardana
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 735 Light Hall, Nashville, Tennessee 37232, USA
In Vitro Cell Dev Biol Anim 41:7-11. 2005..We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells... - Nestin-lineage cells contribute to the microvasculature but not endocrine cells of the isletMary Kay Treutelaar
Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
Diabetes 52:2503-12. 2003..The apparent vascular nature of the nestin-positive cell population and the close association with endocrine cells suggest that nestin-positive cells play an important role in the growth and maintenance of the islet...