Manami Hara

Summary

Affiliation: University of Chicago
Country: USA

Publications

  1. pmc A mouse model for studying intrahepatic islet transplantation
    Manami Hara
    Department of Medicine, University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637, USA
    Transplantation 78:615-8. 2004
  2. ncbi request reprint Imaging pancreatic beta-cells in the intact pancreas
    Manami Hara
    Dept of Medicine, University of Chicago, 5841 South Maryland Ave, MC1027, Chicago, IL 60637, USA
    Am J Physiol Endocrinol Metab 290:E1041-7. 2006
  3. pmc High resolution optical imaging of infarction in intact organs
    Manami Hara
    Department of Medicine, The University of Chicago, Chicago, IL 60637, USA
    Biotechniques 39:373-6. 2005
  4. ncbi request reprint Identification of three missense mutations in the peroxisome proliferator-activated receptor alpha gene in Japanese subjects with maturity-onset diabetes of the young
    M Hara
    Howard Hughes Medical Institute, The University of Chicago, IL 60637, USA
    J Hum Genet 46:285-8. 2001
  5. ncbi request reprint Sustained expression of hepatocyte nuclear factor-6 leads to loss of pancreatic beta-cells by apoptosis
    M Hara
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, United States
    Exp Clin Endocrinol Diabetes 115:654-61. 2007
  6. ncbi request reprint Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells
    Manami Hara
    Department of Medicine, The University of Chicago, Illinois 60637, USA
    Am J Physiol Endocrinol Metab 284:E177-83. 2003
  7. ncbi request reprint Recovery of islet beta-cell function in streptozotocin- induced diabetic mice: an indirect role for the spleen
    Dengping Yin
    Section of Transplantation, Department of Surgery, The University of Chicago, Chicago, IL 60637, USA
    Diabetes 55:3256-63. 2006
  8. ncbi request reprint Relationship of calpain-10 genotype to phenotypic features of polycystic ovary syndrome
    David A Ehrmann
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    J Clin Endocrinol Metab 87:1669-73. 2002
  9. ncbi request reprint Association of the (AU)AT-rich element polymorphism in PPP1R3 with hormonal and metabolic features of polycystic ovary syndrome
    Sergio Y Alcoser
    Department of Biochemistry, The University of Chicago, Chicago, Illinois 60637, USA
    J Clin Endocrinol Metab 89:2973-6. 2004
  10. ncbi request reprint Reversal of diabetes in non-obese diabetic mice without spleen cell-derived beta cell regeneration
    Anita S Chong
    Section of Transplantation, Department of Surgery, University of Chicago, Chicago, IL 60637, USA
    Science 311:1774-5. 2006

Collaborators

Detail Information

Publications38

  1. pmc A mouse model for studying intrahepatic islet transplantation
    Manami Hara
    Department of Medicine, University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637, USA
    Transplantation 78:615-8. 2004
    ..Initial events 24 hr after islet transplantation in the liver include beta-cell loss and hepatic ischemic injuries...
  2. ncbi request reprint Imaging pancreatic beta-cells in the intact pancreas
    Manami Hara
    Dept of Medicine, University of Chicago, 5841 South Maryland Ave, MC1027, Chicago, IL 60637, USA
    Am J Physiol Endocrinol Metab 290:E1041-7. 2006
    ....
  3. pmc High resolution optical imaging of infarction in intact organs
    Manami Hara
    Department of Medicine, The University of Chicago, Chicago, IL 60637, USA
    Biotechniques 39:373-6. 2005
    ....
  4. ncbi request reprint Identification of three missense mutations in the peroxisome proliferator-activated receptor alpha gene in Japanese subjects with maturity-onset diabetes of the young
    M Hara
    Howard Hughes Medical Institute, The University of Chicago, IL 60637, USA
    J Hum Genet 46:285-8. 2001
    ..Variation in the coding region of PPARA is unlikely to be a major cause of MODY in Japanese people...
  5. ncbi request reprint Sustained expression of hepatocyte nuclear factor-6 leads to loss of pancreatic beta-cells by apoptosis
    M Hara
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, United States
    Exp Clin Endocrinol Diabetes 115:654-61. 2007
    ..This severe loss of beta-cells involved programmed cell death...
  6. ncbi request reprint Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells
    Manami Hara
    Department of Medicine, The University of Chicago, Illinois 60637, USA
    Am J Physiol Endocrinol Metab 284:E177-83. 2003
    ..4 +/- 0.2% (n = 6) of the cells in the pancreas of newborn (P1) and 0.9 +/- 0.1% (n = 5) of 8-wk-old mice were beta-cells. The MIP-GFP-transgenic mouse may be a useful tool for studying beta-cell biology in normal and diabetic animals...
  7. ncbi request reprint Recovery of islet beta-cell function in streptozotocin- induced diabetic mice: an indirect role for the spleen
    Dengping Yin
    Section of Transplantation, Department of Surgery, The University of Chicago, Chicago, IL 60637, USA
    Diabetes 55:3256-63. 2006
    ..This study supports the possibility of restoring beta-cell function in diabetic individuals and points to a role for the spleen in facilitating this process...
  8. ncbi request reprint Relationship of calpain-10 genotype to phenotypic features of polycystic ovary syndrome
    David A Ehrmann
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    J Clin Endocrinol Metab 87:1669-73. 2002
    ..In addition to its association with insulin levels in African-Americans, the 112/121-haplotype combination was associated with an approximate 2-fold increase in risk of PCOS in both African-Americans and whites...
  9. ncbi request reprint Association of the (AU)AT-rich element polymorphism in PPP1R3 with hormonal and metabolic features of polycystic ovary syndrome
    Sergio Y Alcoser
    Department of Biochemistry, The University of Chicago, Chicago, Illinois 60637, USA
    J Clin Endocrinol Metab 89:2973-6. 2004
    ..8 pmol/liter). Studies of diabetic and nondiabetic PCOS women of other racial and ethnic backgrounds will be necessary to assess the impact of this and other variants in PPP1R3 upon the phenotype and natural history of women with PCOS...
  10. ncbi request reprint Reversal of diabetes in non-obese diabetic mice without spleen cell-derived beta cell regeneration
    Anita S Chong
    Section of Transplantation, Department of Surgery, University of Chicago, Chicago, IL 60637, USA
    Science 311:1774-5. 2006
    ..Using the same approach, we report here that this treatment cured 32% of NOD mice of established diabetes (>340 milligrams per deciliter blood glucose), although beta cells in these mice were not derived from donor splenocytes...
  11. pmc Quantification of pancreatic islet distribution in situ in mice
    German Kilimnik
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    Am J Physiol Endocrinol Metab 297:E1331-8. 2009
    ....
  12. pmc Islet formation during the neonatal development in mice
    Kevin Miller
    Department of Medicine, The University of Chicago, Chicago, IL, USA
    PLoS ONE 4:e7739. 2009
    ..Mathematical modeling of the fission process in the neonatal islet formation is also presented...
  13. pmc Islet architecture: A comparative study
    Abraham Kim
    Department of Medicine, The University of Chicago, Chicago, IL, USA
    Islets 1:129-36. 2009
    ..In summary, human and mouse islets share common architectural features that may reflect demand for insulin. Comparative studies of islet architecture may lead to a better understanding of islet development and function...
  14. pmc Quantitative analysis of pancreatic polypeptide cell distribution in the human pancreas
    Xiaojun Wang
    Department of Surgery, The University of Chicago, Chicago, Illinois, United States of America
    PLoS ONE 8:e55501. 2013
    ..This study presents an important implication for the regional selection and interpretation of the results...
  15. pmc Altered islet composition and disproportionate loss of large islets in patients with type 2 diabetes
    German Kilimnik
    Department of Medicine, The University of Chicago, Chicago, Illinois, United States of America
    PLoS ONE 6:e27445. 2011
    ....
  16. ncbi request reprint Imaging endoplasmic reticulum calcium with a fluorescent biosensor in transgenic mice
    Manami Hara
    Department of Medicine MC1027, The University of Chicago, 5841 South Maryland Ave, Chicago, IL 60637, USA
    Am J Physiol Cell Physiol 287:C932-8. 2004
    ....
  17. pmc Intraislet production of GLP-1 by activation of prohormone convertase 1/3 in pancreatic α-cells in mouse models of ß-cell regeneration
    German Kilimnik
    Department of Medicine, The University of Chicago, Chicago, IL USA
    Islets 2:149-55. 2010
    ..Our study suggests an important role of α-cells for ß-cell proliferation and further for the endocrine cell network within an islet...
  18. ncbi request reprint Insulin resistance is attenuated in women with polycystic ovary syndrome with the Pro(12)Ala polymorphism in the PPARgamma gene
    Manami Hara
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Clin Endocrinol Metab 87:772-5. 2002
    ..03) and 2 h (688 +/- 103 vs. 10190 +/- 99 pmol/liter; P = 0.04) time points during the oral glucose tolerance test. We conclude that Pro(12)Ala in PPARG is a modifier of insulin resistance in Caucasian women with PCOS...
  19. pmc GSK-3 inactivation or depletion promotes β-cell replication via down regulation of the CDK inhibitor, p27 (Kip1)
    Jeffrey Stein
    Section of Adult and Pediatric Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Chicago, Chicago, IL, USA
    Islets 3:21-34. 2011
    ..Altogether our findings indicate that p27 levels in β-cells are stabilized by GSK-3 and thus p27 down regulation following GSK-3 depletion / inactivation plays a critical role in promoting β-cell replication...
  20. pmc Regional differences in islet distribution in the human pancreas--preferential beta-cell loss in the head region in patients with type 2 diabetes
    Xiaojun Wang
    Department of Surgery, The University of Chicago, Chicago, Illinois, United States of America
    PLoS ONE 8:e67454. 2013
    ....
  21. pmc Quantification of islet size and architecture
    German Kilimnik
    Department of Medicine, The University of Chicago, Chicago, IL, USA
    Islets 4:167-72. 2012
    ..In this article, we describe the method of image capture, parameters measured, data analysis and interpretation of the data...
  22. pmc Role of HNF-1alpha in regulating the expression of genes involved in cellular growth and proliferation in pancreatic beta-cells
    Yuji Uchizono
    Department of Medicine, The University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637, USA
    Diabetes Res Clin Pract 84:19-26. 2009
    ..These results provide insights into the mechanisms whereby HNF-1alpha affects beta-cell function...
  23. pmc Extrapancreatic insulin-producing cells in multiple organs in diabetes
    Hideto Kojima
    Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 101:2458-63. 2004
    ..These observations have implications for the regulation of insulin gene expression, modulation of self-tolerance by insulin gene expression, and strategies for the generation of insulin-producing cells for the treatment of diabetes...
  24. pmc Generation of embryonic stem cells from mouse insulin I promoter-green fluorescent protein transgenic mice and characterization in a teratoma model
    Wieslawa M Milewski
    Department of Medicine, The University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637, USA
    In Vitro Cell Dev Biol Anim 45:1-5. 2009
    ..The MIP-GFP ESCs should be a useful research tool to study the differentiation capacity of ESCs toward pancreatic lineages...
  25. pmc No mantle formation in rodent islets -- the prototype of islet revisited
    Michael Kharouta
    Department of Medicine, The University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637, United States
    Diabetes Res Clin Pract 85:252-7. 2009
    ..In summary, human and mouse islets share common architectural features as endocrine micro-organs. Since these may hold a key to better understanding islet plasticity, our concept of the prototypic islet should be revised...
  26. doi request reprint Coating human pancreatic islets with CD4(+)CD25(high)CD127(-) regulatory T cells as a novel approach for the local immunoprotection
    Natalia Marek
    Department of Surgery, Section of Transplantation, University of Chicago, IL 60637, USA
    Ann Surg 254:512-8; discussion 518-9. 2011
    ..To develop a novel approach for local immunoprotection using CD4(+)CD25(high)CD127(-) T regulatory cells (Tregs) attached to the surface of the islets before transplantation...
  27. pmc Pancreatic islet plasticity: interspecies comparison of islet architecture and composition
    Donald J Steiner
    Department of Medicine, The University of Chicago, Chicago, IL USA
    Islets 2:135-45. 2010
    ..We propose that this plasticity reflects evolutionary acquired adaptation induced by altered physiological conditions, rather than inherent disparities between species...
  28. ncbi request reprint Beta-cell transcription factors and diabetes: mutations in the coding region of the BETA2/NeuroD1 (NEUROD1) and Nkx2.2 (NKX2B) genes are not associated with maturity-onset diabetes of the young in Japanese
    H Furuta
    Department of Biochemistry and Molecular Biology, The University of Chicago, Illinois 60637, USA
    Diabetes 47:1356-8. 1998
  29. ncbi request reprint Familial juvenile autoimmune hypothyroidism, pituitary enlargement, obesity, and insulin resistance
    Sirimon Reutrakul
    The J P Kennedy Jr Mental Retardation Research Center, University of Chicago, Illinois 60637, USA
    Thyroid 14:311-9. 2004
    ..In conclusion, the remarkably high incidence of childhood autoimmune hypothyroidism, pituitary enlargement, insulin resistance and obesity in this family is not linked to known HLA types or known gene defects...
  30. ncbi request reprint Liver ischemia contributes to early islet failure following intraportal transplantation: benefits of liver ischemic-preconditioning
    D Yin
    Section of Transplantation, Department of Surgery, The University of Chicago, Chicago, Illinois, USA
    Am J Transplant 6:60-8. 2006
    ..These data suggest that islet embolism in the portal vein is a major cause of functional loss following IPIT that can be prevented by liver IP...
  31. ncbi request reprint Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitus
    Y Horikawa
    Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA
    Nat Genet 26:163-75. 2000
    ..This finding suggests a novel pathway that may contribute to the development of type 2 diabetes...
  32. ncbi request reprint Cloning of rat and mouse P2Y purinoceptors
    Y Tokuyama
    Department of Medicine, Howard Hughes Medical Institute, University of Chicago, Illinois 60637, USA
    Biochem Biophys Res Commun 211:211-8. 1995
    ..The cloned rat P2Y purinoceptor was expressed in Xenopus laevis oocytes and possessed the properties expected for this receptor subtype...
  33. pmc Glial cell line-derived neurotrophic factor increases beta-cell mass and improves glucose tolerance
    Simon Mwangi
    Division of Digestive Diseases, Emory University, Atlanta, Georgia, USA
    Gastroenterology 134:727-37. 2008
    ..Glial cell line-derived neurotrophic factor (GDNF) is a growth factor that plays a role in the development and survival of the enteric nervous system. We investigated the role of GDNF in regulating beta-cell survival...
  34. pmc The fusion of bone-marrow-derived proinsulin-expressing cells with nerve cells underlies diabetic neuropathy
    Tomoya Terashima
    Department of Molecular Genetics in Medicine, Shiga University of Medical Science, Otsu, Shiga 520 2192, Japan
    Proc Natl Acad Sci U S A 102:12525-30. 2005
    ..These results suggest that bone-marrow-derived cells may play an important role in the pathogenesis of diabetic complications...
  35. ncbi request reprint Electrophysiological characterization of pancreatic islet cells in the mouse insulin promoter-green fluorescent protein mouse
    Yuk M Leung
    Department of Medicine, Room 7308, or Dr Herbert Y Gaisano, Room 7226, Medical Sciences Building, 1 King s College Circle, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Endocrinology 146:4766-75. 2005
    ..The MIP-GFP mouse islet is therefore a reliable model to efficiently examine alpha-cell and beta-cell physiology and should greatly facilitate examination of their pathophysiology when the MIP-GFP mice are crossed with diabetic models...
  36. ncbi request reprint Imaging Beta cell development in real-time using pancreatic explants from mice with green fluorescent protein-labeled pancreatic Beta cells
    Subhadra C Gunawardana
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 735 Light Hall, Nashville, Tennessee 37232, USA
    In Vitro Cell Dev Biol Anim 41:7-11. 2005
    ..We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells...
  37. ncbi request reprint Nestin-lineage cells contribute to the microvasculature but not endocrine cells of the islet
    Mary Kay Treutelaar
    Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
    Diabetes 52:2503-12. 2003
    ..The apparent vascular nature of the nestin-positive cell population and the close association with endocrine cells suggest that nestin-positive cells play an important role in the growth and maintenance of the islet...
  38. ncbi request reprint Insulin regulates islet alpha-cell function by reducing KATP channel sensitivity to adenosine 5'-triphosphate inhibition
    Yuk M Leung
    Departments of Medicine and Physiology, University of Toronto, Canada
    Endocrinology 147:2155-62. 2006
    ..This may be an underlying basis for the proposed sequential glucose-insulin regulation of alpha-cell glucagon secretion, which becomes distorted in diabetes, leading to dysregulated glucagon secretion...