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Genomes and Genes | Andrew D HansonSummaryAffiliation: University of Florida Country: USA Publications
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Publications
Metabolism of the folate precursor p-aminobenzoate in plants: glucose ester formation and vacuolar storageAymerick Eudes
Departments of Horticultural Sciences and Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611, USA
J Biol Chem 283:15451-9. 2008..In total, these data and the fact that isolated mitochondria did not take up [(3)H]pABA-Glc, suggest that the glucose ester represents a storage form of pABA that does not contribute directly to folate synthesis...- Synergistic use of plant-prokaryote comparative genomics for functional annotationsSvetlana Gerdes
Fellowship for Interpretation of Genomes, Burr Ridge, IL, USA
BMC Genomics 12:S2. 2011..This approach bridges the gap between automated homology-based annotations and the classical gene discovery efforts of experimentalists, and is more powerful than purely computational approaches to identifying gene-function associations... 
Synthesis and turnover of folates in plantsAndrew D Hanson
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
Curr Opin Plant Biol 5:244-9. 2002..Moreover, new analytical advances will help to elucidate plant folate turnover and transport, which are practically unexplored...- Folate synthesis in plants: the last step of the p-aminobenzoate branch is catalyzed by a plastidial aminodeoxychorismate lyaseGilles J C Basset
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Plant J 40:453-61. 2004..As shown previously for the ADC synthase transcript, the level of ADC lyase mRNA in the pericarp of tomato fruit falls sharply as ripening advances, suggesting that the expression of these two enzymes is coregulated... - Higher plant plastids and cyanobacteria have folate carriers related to those of trypanosomatidsSebastian M J Klaus
Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 280:38457-63. 2005..These findings establish conservation of function among folate and biopterin transporter family proteins from three kingdoms of life... - 5-Formyltetrahydrofolate is an inhibitory but well tolerated metabolite in Arabidopsis leavesAymeric Goyer
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 280:26137-42. 2005..However, the near-normal growth of the mutant shows that even exceptionally high 5-CHO-THF levels do not much affect fluxes through SHMT or any other folate-dependent reaction, i.e. that 5-CHO-THF is well tolerated in plants... - Identification of transport-critical residues in a folate transporter from the folate-biopterin transporter (FBT) familyAymerick Eudes
Horticultural Sciences, Human Nutrition Department, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 285:2867-75. 2010..coli. Such negative data could reflect roles in transport of other substrates. These studies provide the first insights into the native structure and catalytic mechanism of FBT family carriers... - 6-pyruvoyltetrahydropterin synthase paralogs replace the folate synthesis enzyme dihydroneopterin aldolase in diverse bacteriaAnne Pribat
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
J Bacteriol 191:4158-65. 2009.... 
A 5-formyltetrahydrofolate cycloligase paralog from all domains of life: comparative genomic and experimental evidence for a cryptic role in thiamin metabolismAnne Pribat
Horticultural Sciences Department, University of Florida, Gainesville, 32611, USA
Funct Integr Genomics 11:467-78. 2011..We therefore propose (a) that COG0212 has an unrecognized yet sometimes crucial role in thiamin metabolism, most probably in salvage or detoxification, and (b) that is not a 5-FCL and should no longer be so annotated...- FolX and FolM are essential for tetrahydromonapterin synthesis in Escherichia coli and Pseudomonas aeruginosaAnne Pribat
University of Florida, Horticultural Sciences Department, Gainesville, FL 32611, USA
J Bacteriol 192:475-82. 2010.... - Characterization of the folate salvage enzyme p-aminobenzoylglutamate hydrolase in plantsGale G Bozzo
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, United States
Phytochemistry 69:29-37. 2008..The finding that Arabidopsis has folate-hydrolyzing activity points to an enzymatic component of folate degradation in plants... - A role for tetrahydrofolates in the metabolism of iron-sulfur clusters in all domains of lifeJeffrey C Waller
Horticultural Sciences, Food Science and Human Nutrition, Microbiology and Cell Science Departments, and Department of Biochemistry and Molecular Biology and National High Magnetic Field Laboratory, and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA
Proc Natl Acad Sci U S A 107:10412-7. 2010..Thus COG0354 proteins have an ancient, conserved, folate-dependent function in the activity of certain Fe/S cluster enzymes... - Folate biosynthesis in higher plants. cDNA cloning, heterologous expression, and characterization of dihydroneopterin aldolasesAymeric Goyer
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 135:103-11. 2004..The substantial divergence of FolB homologs in Arabidopsis and other plants suggests that some of them may act on substrates other than DHN... - A central role for gamma-glutamyl hydrolases in plant folate homeostasisTariq A Akhtar
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Plant J 64:256-66. 2010.... - Tomato gamma-glutamylhydrolases: expression, characterization, and evidence for heterodimer formationTariq A Akhtar
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Plant Physiol 148:775-85. 2008..Plant GGH heterodimers thus appear to form wherever different GGH genes are expressed simultaneously and to have catalytic characteristics midway between those of the corresponding homodimers... - Moonlighting glutamate formiminotransferases can functionally replace 5-formyltetrahydrofolate cycloligaseLinda Jeanguenin
Department of Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 285:41557-66. 2010..Collectively, these data indicate that FTs functionally replace 5-FCL in certain prokaryotes... - Mutational analysis of YgfZ, a folate-dependent protein implicated in iron/sulphur cluster metabolismGhulam Hasnain
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, USA
FEMS Microbiol Lett 326:168-72. 2012..Collectively, these data demonstrate a pivotal role for the Ygf Z motif's cysteine residue and a subsidiary one for the adjacent tyrosine, and help formulate a hypothesis about the folate requirement of Ygf Z proteins... - Plant gamma-glutamyl hydrolases and folate polyglutamates: characterization, compartmentation, and co-occurrence in vacuolesGiuseppe Orsomando
Horticultural Sciences and Food Science Department, University of Florida, Gainesville, FL 32611, USA
J Biol Chem 280:28877-84. 2005..These data point to a hitherto unsuspected role for vacuoles in folate storage. Furthermore, the paradoxical co-occurrence of GGH and folylpolyglutamates in vacuoles implies that the polyglutamates are somehow protected from GGH attack... - Pterin and folate salvage. Plants and Escherichia coli lack capacity to reduce oxidized pterinsAlexandre Noiriel
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 143:1101-9. 2007..If it is lacking, folate precursors or breakdown products that become oxidized will permanently exit the metabolically active pterin pool... - Evidence for folate-salvage reactions in plantsGiuseppe Orsomando
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Plant J 46:426-35. 2006..We conclude that plants are excellent organisms in which to explore the biochemistry of folate salvage... - Mitochondrial and plastidial COG0354 proteins have folate-dependent functions in iron-sulphur cluster metabolismJeffrey C Waller
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
J Exp Bot 63:403-11. 2012..That plants retain mitochondrial and plastidial COG0354 proteins with distinct phylogenetic origins emphasizes how deeply the extant Fe/S cluster assembly machinery still reflects the ancient endosymbioses that gave rise to plants... - Comparative genomics and functional analysis of the NiaP family uncover nicotinate transporters from bacteria, plants, and mammalsLinda Jeanguenin
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Funct Integr Genomics 12:25-34. 2012..More generally, these results indicate that specificity for nicotinate is conserved widely, but not absolutely, among pro- and eukaryotic NiaP family proteins... - Functional characterization of a methionine gamma-lyase in Arabidopsis and its implication in an alternative to the reverse trans-sulfuration pathwayAymeric Goyer
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Plant Cell Physiol 48:232-42. 2007..These data indicate that plants possess an alternative to the reverse trans-sulfuration pathway (methionine-->homocysteine-->cystathionine-->cysteine) in which methanethiol is an intermediate... - Characterization and metabolic function of a peroxisomal sarcosine and pipecolate oxidase from ArabidopsisAymeric Goyer
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 279:16947-53. 2004..These data indicate that pipecolate is the endogenous substrate for SOX in plants and that plants can utilize exogenous sarcosine opportunistically, sarcosine being a common soil metabolite... - Folate synthesis in plants: the first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase IGilles Basset
Horticultural Sciences Department and Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA
Proc Natl Acad Sci U S A 99:12489-94. 2002..As ripening advances, GCHI expression declines sharply, and folate content drops, suggesting that folate synthesis fails to keep pace with turnover... - Developmental and feedforward control of the expression of folate biosynthesis genes in tomato fruitJeffrey C Waller
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Mol Plant 3:66-77. 2010..Microarray analysis showed that only 14 other transcripts (of 11 000 surveyed) increased in abundance by two-fold or more in high-folate fruit, demonstrating that the induction of folate pathway genes is relatively specific... - Nonflowering plants possess a unique folate-dependent phenylalanine hydroxylase that is localized in chloroplastsAnne Pribat
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
Plant Cell 22:3410-22. 2010..These data show that nonflowering plants have functional plastidial AAHs, establish an unprecedented electron donor role for a folate, and uncover a novel link between folate and aromatic metabolism... - A nudix enzyme removes pyrophosphate from dihydroneopterin triphosphate in the folate synthesis pathway of bacteria and plantsSebastian M J Klaus
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
J Biol Chem 280:5274-80. 2005..and Kraszewska, E. (2002) J. Biol. Chem. 277, 50482-50486). However, we found that this activity is negligible at physiological levels of Mn(2+) and that, with 1 mM Mg(2+), AtNUDT1 prefers DHNTP and (deoxy) nucleoside triphosphates... - Identification of mitochondrial thiamin diphosphate carriers from Arabidopsis and maizeOcéane Frelin
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Funct Integr Genomics 12:317-26. 2012..Our data provide a firm basis to propagate the functional annotation of mitochondrial ThDP carriers to other angiosperm genomes... - The folate precursor p-aminobenzoate is reversibly converted to its glucose ester in the plant cytosolEoin P Quinlivan
Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 278:20731-7. 2003..Esterification of pABA may eliminate such errant behavior and provide a readily reclaimable storage form of pABA as well as a substrate for membrane transporters... - Mitochondrial phosphatidylserine decarboxylase from higher plants. Functional complementation in yeast, localization in plants, and overexpression in ArabidopsisDenis Rontein
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 132:1678-87. 2003..They also indicate that mitochondrial PSD is an important housekeeping enzyme whose expression is strongly regulated at the transcriptional level... - Folate salvage in plants: pterin aldehyde reduction is mediated by multiple non-specific aldehyde reductasesAlexandre Noiriel
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Plant J 51:378-89. 2007..We conclude that pterin aldehyde salvage in plants involves multiple, generalist NADPH-linked reductases, and that the At1g10310 enzyme is typical of these and hence suitable for use in engineering studies of folate turnover... - Folate biofortification of tomato fruitRocio I Díaz De La Garza
Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USA
Proc Natl Acad Sci U S A 104:4218-22. 2007..The folate levels we achieved provide the complete adult daily requirement in less than one standard serving... - Folate biofortification in tomatoes by engineering the pteridine branch of folate synthesisRocio Diaz De La Garza
Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USA
Proc Natl Acad Sci U S A 101:13720-5. 2004..These results demonstrate that engineering a moderate increase in pteridine production can significantly enhance the folate content in food plants and that boosting the PABA supply can produce further gains... - Comparative genomics of bacterial and plant folate synthesis and salvage: predictions and validationsVALERIE DE CRECY-LAGARD
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA
BMC Genomics 8:245. 2007..This study reports the results of the analysis and integrates them with new and existing experimental data... - Phylogenomic and functional analysis of pterin-4a-carbinolamine dehydratase family (COG2154) proteins in plants and microorganismsValeria Naponelli
Department of Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 146:1515-27. 2008..Besides this role, we propose that partnerless PCDs support the function of as yet unrecognized pterin-dependent enzymes... - Evidence that the folate-dependent proteins YgfZ and MnmEG have opposing effects on growth and on activity of the iron-sulfur enzyme MiaBJeffrey C Waller
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida, USA
J Bacteriol 194:362-7. 2012..A more plausible explanation is that MnmEG erroneously transfers a folate-bound formaldehyde unit to MiaB and that YgfZ reverses this... - 'Unknown' proteins and 'orphan' enzymes: the missing half of the engineering parts list--and how to find itAndrew D Hanson
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Biochem J 425:1-11. 2010..Furthermore, it is cost-efficient and increasingly straightforward to deploy owing to a proliferation of databases and associated tools... - Improved methods for the preparation of [3H]folate polyglutamates: biosynthesis with Lactobacillus casei and enzymatic synthesis with Escherichia coli folylpolyglutamate synthetaseValeria Naponelli
Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611, USA
Anal Biochem 371:127-34. 2007..This enzymatic synthesis yielded predominantly tri-, tetra-, and pentaglutamyl species for the [(3)H]folate substrate... - Folate synthesis in plants: the p-aminobenzoate branch is initiated by a bifunctional PabA-PabB protein that is targeted to plastidsGilles J C Basset
Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USA
Proc Natl Acad Sci U S A 101:1496-501. 2004..In the pericarp of tomato fruit, the PabA-PabB mRNA level fell drastically as ripening advanced, but there was no fall in total PABA content, which stayed between 0.7 and 2.3 nmol.g(-1) fresh weight... - Identification of genes encoding the folate- and thiamine-binding membrane proteins in FirmicutesAymerick Eudes
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
J Bacteriol 190:7591-4. 2008..Similar genes occur in many Firmicutes, sometimes next to folate or thiamine salvage genes. Most thiT genes are preceded by a thiamine riboswitch... - Finding novel metabolic genes through plant-prokaryote phylogenomicsVALERIE DE CRECY-LAGARD
Microbiology and Cell Science Department, University of Florida, Gainesville, FL 32611, USA
Trends Microbiol 15:563-70. 2007..This breaks with the past pattern of gene discovery, in which the information flow was most often unidirectional from prokaryotes to plants... - Identification and characterization of the missing pyrimidine reductase in the plant riboflavin biosynthesis pathwayGhulam Hasnain
Department of Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 161:48-56. 2013..The role of the COG3236 domain remains mysterious; no evidence was obtained for the possibility that it catalyzes the dephosphorylation that follows pyrimidine reduction... - Folate biosynthesis, turnover, and transport in plantsAndrew D Hanson
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
Annu Rev Plant Biol 62:105-25. 2011..Understanding these processes, which will require both biochemical and -omics research, should lead to improved biofortification strategies based on transgenic or conventional approaches... - Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehydeDenise Tieman
Department of Horticultural Sciences, University of Florida, P O Box 110690, Gainesville, FL 32611 0690, USA
Proc Natl Acad Sci U S A 103:8287-92. 2006..Besides establishing a biosynthetic route, these results show that it is possible to change phenylalanine-based flavor and aroma volatiles in plants by manipulating expression of a single gene... - Evidence from engineering that decarboxylation of free serine is the major source of ethanolamine moieties in plantsDenis Rontein
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611-0690, U.S.A
Plant Cell Physiol 44:1185-91. 2003..Taken together, these data indicate that serine decarboxylation is the main source of Etn moieties in plants. The ability to enhance serine --> Etn flux should advance engineering of choline and glycine betaine accumulation... - Cloning and characterization of mitochondrial 5-formyltetrahydrofolate cycloligase from higher plantsSanja Roje
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 277:42748-54. 2002..Consistent with this possibility, 5-FCL activity was far higher in leaf mitochondria than root mitochondria... - The analysis of folate and its metabolic precursors in biological samplesEoin P Quinlivan
Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611, USA
Anal Biochem 348:163-84. 2006 - Insertional inactivation of the methionine s-methyltransferase gene eliminates the s-methylmethionine cycle and increases the methylation ratioMichael G Kocsis
Horticultural Sciences Department, University of Florida, Gainesville 32611, USA
Plant Physiol 131:1808-15. 2003..These data indicate that the SMM cycle contributes to regulation of Ado-Met levels rather than preventing depletion of free Met... - Metabolic engineering in yeast demonstrates that S-adenosylmethionine controls flux through the methylenetetrahydrofolate reductase reaction in vivoSanja Roje
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 277:4056-61. 2002..Possibly related to this shift in one-carbon fluxes, total folate levels are doubled in yeast cells expressing Chimera-1... - Metabolic engineering of osmoprotectant accumulation in plantsDenis Rontein
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
Metab Eng 4:49-56. 2002.... - Folate synthesis in plants: purification, kinetic properties, and inhibition of aminodeoxychorismate synthaseTobias Sahr
, , , CEA-Grenoble, 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France
Biochem J 396:157-62. 2006..These findings support the view that the monomeric ADC synthase is a potential target for antifolate drugs... - Folate biofortification in food plantsSamir Bekaert
Department of Molecular Genetics, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
Trends Plant Sci 13:28-35. 2008..Here, we review the current status of investigations in the field of folate enhancement on the eve of a new era in food fortification... - Deficiency in phosphatidylserine decarboxylase activity in the psd1 psd2 psd3 triple mutant of Arabidopsis affects phosphatidylethanolamine accumulation in mitochondriaAnnika Nerlich
Max Planck Institute of Molecular Plant Physiology, Department of Molecular Physiology, 14476 Potsdam, Germany
Plant Physiol 144:904-14. 2007..These results imply that, similar to yeast and animal cells, a specific phospholipid transfer from the endoplasmic reticulum to mitochondria exists in plants...