JO E HANDELSMAN

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Metagenomics for studying unculturable microorganisms: cutting the Gordian knot
    Patrick D Schloss
    Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA
    Genome Biol 6:229. 2005
  2. ncbi request reprint Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products
    J Handelsman
    Department of Plant Pathology, University of Wisconsin Madison 53706, USA
    Chem Biol 5:R245-9. 1998
  3. pmc Metagenomics: application of genomics to uncultured microorganisms
    Jo Handelsman
    Department of Plant Pathology, University of Wisconsin Madison, Madison, WI 53706, USA
    Microbiol Mol Biol Rev 68:669-85. 2004
  4. ncbi request reprint NIH Director's Pioneer Awards: could the selection process be biased against women?
    Molly Carnes
    Department of Medicine and Center for Women s Health Research, Medical School, University of Wisconsin Madison, Meriter Hospital, 6 West, 202 South Park Street, Madison, WI 53715, USA
    J Womens Health (Larchmt) 14:684-91. 2005
  5. doi request reprint Rules of engagement: interspecies interactions that regulate microbial communities
    Ainslie E F Little
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, 53706, USA
    Annu Rev Microbiol 62:375-401. 2008
  6. doi request reprint Searching for excellence & diversity: increasing the hiring of women faculty at one academic medical center
    Jennifer T Sheridan
    Women in Science and Engineering Leadership Institute, University of Wisconsin Madison, Madison, WI, USA
    Acad Med 85:999-1007. 2010
  7. pmc Money, sex, and drugs: a case study to teach the genetics of antibiotic resistance
    Karen A Cloud-Hansen
    Wisconsin Program for Scientific Teaching, Department of Bacteriology, Center for Biology Education, University of Wisconsin Madison, Madison, WI 53706, USA
    CBE Life Sci Educ 7:302-9. 2008
  8. pmc Signal mimics derived from a metagenomic analysis of the gypsy moth gut microbiota
    Changhui Guan
    Department of Plant Pathology, University of Wisconsin Madison, WI 53706, USA
    Appl Environ Microbiol 73:3669-76. 2007
  9. ncbi request reprint Diversity in academic medicine: the stages of change model
    Molly Carnes
    Department of Medicine, Center for Women s Health Research, Women in Science and Engineering Leadership Institute WISELI, University of Wisconsin, Madison, Wisconsin 53715, USA
    J Womens Health (Larchmt) 14:471-5. 2005
  10. pmc Robustness of the bacterial community in the cabbage white butterfly larval midgut
    Courtney J Robinson
    Microbiology Doctoral Training Program, University of Wisconsin Madison, Madison, WI 53706, USA
    Microb Ecol 59:199-211. 2010

Collaborators

Detail Information

Publications44

  1. pmc Metagenomics for studying unculturable microorganisms: cutting the Gordian knot
    Patrick D Schloss
    Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA
    Genome Biol 6:229. 2005
    ..Recent advances in shotgun sequencing and computational methods for genome assembly have advanced the field of metagenomics to provide glimpses into the life of uncultured microorganisms...
  2. ncbi request reprint Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products
    J Handelsman
    Department of Plant Pathology, University of Wisconsin Madison 53706, USA
    Chem Biol 5:R245-9. 1998
    ..The concept of cloning the metagenome to access the collective genomes and the biosynthetic machinery of soil microflora is explored here...
  3. pmc Metagenomics: application of genomics to uncultured microorganisms
    Jo Handelsman
    Department of Plant Pathology, University of Wisconsin Madison, Madison, WI 53706, USA
    Microbiol Mol Biol Rev 68:669-85. 2004
    ..The application of metagenomic sequence information will facilitate the design of better culturing strategies to link genomic analysis with pure culture studies...
  4. ncbi request reprint NIH Director's Pioneer Awards: could the selection process be biased against women?
    Molly Carnes
    Department of Medicine and Center for Women s Health Research, Medical School, University of Wisconsin Madison, Meriter Hospital, 6 West, 202 South Park Street, Madison, WI 53715, USA
    J Womens Health (Larchmt) 14:684-91. 2005
    ..We offer an analysis of this process to encourage the NIH to embark on self-study and to educate all reviewers regarding an evidence-based approach to gender and evaluation...
  5. doi request reprint Rules of engagement: interspecies interactions that regulate microbial communities
    Ainslie E F Little
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, 53706, USA
    Annu Rev Microbiol 62:375-401. 2008
    ..We describe current approaches to study microbial ecology and progress toward predictive modeling...
  6. doi request reprint Searching for excellence & diversity: increasing the hiring of women faculty at one academic medical center
    Jennifer T Sheridan
    Women in Science and Engineering Leadership Institute, University of Wisconsin Madison, Madison, WI, USA
    Acad Med 85:999-1007. 2010
    ..The authors articulate successful elements of workshop implementation for other medical schools seeking to increase gender diversity on their faculties...
  7. pmc Money, sex, and drugs: a case study to teach the genetics of antibiotic resistance
    Karen A Cloud-Hansen
    Wisconsin Program for Scientific Teaching, Department of Bacteriology, Center for Biology Education, University of Wisconsin Madison, Madison, WI 53706, USA
    CBE Life Sci Educ 7:302-9. 2008
    ..Implementation of "Ciprofloxacin Resistance in Neisseria gonorrhoeae" in a college freshman seminar suggests these materials are useful in increasing understanding of complex biological topics and improving problem-solving abilities...
  8. pmc Signal mimics derived from a metagenomic analysis of the gypsy moth gut microbiota
    Changhui Guan
    Department of Plant Pathology, University of Wisconsin Madison, WI 53706, USA
    Appl Environ Microbiol 73:3669-76. 2007
    ..The signal from this clone induces the activities of LuxR from Vibrio fischeri and CviR from Chromobacterium violaceum. This study is the first to identify a new structural class of quorum-sensing inducer from uncultured bacteria...
  9. ncbi request reprint Diversity in academic medicine: the stages of change model
    Molly Carnes
    Department of Medicine, Center for Women s Health Research, Women in Science and Engineering Leadership Institute WISELI, University of Wisconsin, Madison, Wisconsin 53715, USA
    J Womens Health (Larchmt) 14:471-5. 2005
  10. pmc Robustness of the bacterial community in the cabbage white butterfly larval midgut
    Courtney J Robinson
    Microbiology Doctoral Training Program, University of Wisconsin Madison, Madison, WI 53706, USA
    Microb Ecol 59:199-211. 2010
    ....
  11. pmc Contributions of gut bacteria to Bacillus thuringiensis-induced mortality vary across a range of Lepidoptera
    Nichole A Broderick
    Department of Entomology, University of Wisconsin, Madison, WI 53706, USA
    BMC Biol 7:11. 2009
    ..We then assessed the effect of feeding an enteric bacterium isolated from L. dispar on larval mortality following ingestion of B. thuringiensis toxin...
  12. pmc Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independent methods
    Nichole A Broderick
    Department of Entomology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Appl Environ Microbiol 70:293-300. 2004
    ..These results provide the first comprehensive description of the microbial diversity of a lepidopteran midgut and demonstrate that the plant species in the diet influences the composition of the gut bacterial community...
  13. pmc Midgut bacteria required for Bacillus thuringiensis insecticidal activity
    Nichole A Broderick
    Department of Entomology, University of Wisconsin, 1630 Linden Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 103:15196-9. 2006
    ..E. coli and the Enterobacter sp. achieved high populations in hemolymph, in contrast to B. thuringiensis, which appeared to die in hemolymph. Our results demonstrate that B. thuringiensis-induced mortality depends on enteric bacteria...
  14. pmc Introducing SONS, a tool for operational taxonomic unit-based comparisons of microbial community memberships and structures
    Patrick D Schloss
    Department of Plant Pathology, University of Wisconsin Madison, Madison, WI 53706, USA
    Appl Environ Microbiol 72:6773-9. 2006
    ..Here we present a computer program, SONS, which implements nonparametric estimators for the fraction and richness of OTUs shared between two communities...
  15. ncbi request reprint Metagenomics: genomic analysis of microbial communities
    Christian S Riesenfeld
    Department of Plant Pathology, University of Wisconsin Madison, Wisconsin 53706, USA
    Annu Rev Genet 38:525-52. 2004
    ..This review describes progress toward understanding the biology of uncultured Bacteria, Archaea, and viruses through metagenomic analyses...
  16. pmc Peptidoglycan from Bacillus cereus mediates commensalism with rhizosphere bacteria from the Cytophaga-Flavobacterium group
    Snow Brook Peterson
    Department of Plant Pathology, University of Wisconsin Madison, Madison, WI 53706, USA
    Appl Environ Microbiol 72:5421-7. 2006
    ..cereus grown in root exudate media did not. These results suggest B. cereus and CF rhizosphere bacteria have a commensal relationship in which peptidoglycan produced by B. cereus stimulates the growth of CF bacteria...
  17. doi request reprint Resident microbiota of the gypsy moth midgut harbors antibiotic resistance determinants
    Heather K Allen
    Departments of Bacteriology and Plant Pathology, University of Wisconsin, Madison, Wisconsin, USA
    DNA Cell Biol 28:109-17. 2009
    ..Insect guts are a reservoir of antibiotic resistance genes with the potential for dissemination...
  18. pmc Chemical modulators of the innate immune response alter gypsy moth larval susceptibility to Bacillus thuringiensis
    Nichole A Broderick
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    BMC Microbiol 10:129. 2010
    ..For example, ingestion of Bacillus thuringiensis by larvae of some species of susceptible Lepidoptera can result in normally benign enteric bacteria exerting pathogenic effects...
  19. doi request reprint Functional metagenomics reveals diverse beta-lactamases in a remote Alaskan soil
    Heather K Allen
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    ISME J 3:243-51. 2009
    ..coli without manipulating its gene expression machinery, demonstrating the potential for soil resistance genes to compromise human health, if transferred to pathogens...
  20. ncbi request reprint Gut microbiota of an invasive subcortical beetle, Agrilus planipennis Fairmaire, across various life stages
    Archana Vasanthakumar
    Department of Entomology, 1630 Linden Dr, 345 Russell Labs, University of Wisconsin Madison, Madison, WI 53706, USA
    Environ Entomol 37:1344-53. 2008
    ....
  21. doi request reprint Quorum-sensing signals in the microbial community of the cabbage white butterfly larval midgut
    Bradley R Borlee
    Department of Plant Pathology, University of Wisconsin Madison, Madison, WI 53706, USA
    ISME J 2:1101-11. 2008
    ..aeruginosa PAO1. These results show that AHLs are exchanged among bacteria in the alkaline gut of CWB larvae and contribute to disease caused by P. aeruginosa PAO1...
  22. pmc Miniprimer PCR, a new lens for viewing the microbial world
    Thomas A Isenbarger
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706 1521, USA
    Appl Environ Microbiol 74:840-9. 2008
    ..Deeply divergent sequences were discovered with high frequency and included representatives that define two new division-level taxa, designated CR1 and CR2, suggesting that miniprimer PCR may reveal new dimensions of microbial diversity...
  23. ncbi request reprint Biotechnological prospects from metagenomics
    Patrick D Schloss
    Department of Plant Pathology, Russell Laboratories, University of Wisconsin, 1630 Linden Drive, Madison, WI 53706, USA
    Curr Opin Biotechnol 14:303-10. 2003
    ..New antibiotics and enzymes are among the early discoveries from metagenomics. Future refinement of methods that enrich for genes of particular function will accelerate the rate of discovery of useful molecules...
  24. ncbi request reprint Uncultured soil bacteria are a reservoir of new antibiotic resistance genes
    Christian S Riesenfeld
    Department of Plant Pathology, University of Wisconsin Madison, Madison, WI 53706, USA
    Environ Microbiol 6:981-9. 2004
    ..The results indicate that soil bacteria are a reservoir of antibiotic resistance genes with greater genetic diversity than previously accounted for, and that the diversity can be surveyed by a culture-independent method...
  25. pmc A census of rRNA genes and linked genomic sequences within a soil metagenomic library
    Mark R Liles
    Department of Plant Pathology Gaylord Nelson Institute of Environmental Studies, University of Wisconsin Madison, 1630 Linden Drive, Madison, WI 53706, USA
    Appl Environ Microbiol 69:2684-91. 2003
    ..This study is the first step in using genomics to reveal the physiology of as-yet-uncultured members of the Acidobacterium division...
  26. pmc Status of the microbial census
    Patrick D Schloss
    Department of Plant Pathology, University of Wisconsin Madison, 1630 Linden Dr, Madison, WI 53706, USA
    Microbiol Mol Biol Rev 68:686-91. 2004
    ..The data support previous calls for extensive surveys of multiple chemically disparate environments and of specific phylogenetic groups to advance the census most rapidly...
  27. pmc Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness
    Patrick D Schloss
    Department of Plant Pathology, University of Wisconsin Madison, 1630 Linden Dr, Madison, WI 53706, USA
    Appl Environ Microbiol 71:1501-6. 2005
    ..We anticipate that an equivalent extent of sampling for soil would require sampling more than 10,000 sequences, almost 100 times the size of typical sequence collections obtained from soil...
  28. ncbi request reprint Toward an understanding of microbial communities through analysis of communication networks
    Anne K Dunn
    Department of Plant Pathology, University of Wisconsin Madison, 53706, USA
    Antonie Van Leeuwenhoek 81:565-74. 2002
    ..cereus biocontrol system may provide a model for network communication among organisms that share a habitat and provide a new angle of analysis for understanding the interconnections that define communities...
  29. pmc Intracellular screen to identify metagenomic clones that induce or inhibit a quorum-sensing biosensor
    Lynn L Williamson
    Department of Plant Pathology, University of Wisconsin Madison, 1630 Linden Dr, Madison, WI 53706, USA
    Appl Environ Microbiol 71:6335-44. 2005
    ..Our results demonstrate the power of an intracellular screen to identify functionally active clones and biologically active small molecules in metagenomic libraries...
  30. pmc Introducing TreeClimber, a test to compare microbial community structures
    Patrick D Schloss
    Department of Plant Pathology, University of Wisconsin Madison, Madison, WI 53706, USA
    Appl Environ Microbiol 72:2379-84. 2006
    ..Here we describe our implementation of the parsimony test, TreeClimber, in which we reanalyzed six previously published studies and compared the results of the analysis to those obtained using integral-LIBSHUFF...
  31. ncbi request reprint Careers in science. More women in science
    Jo Handelsman
    Department of Plant Pathology, University of Wisconsin Madison, 1630 Linden Dr, Madison, WI 53706, USA joh plantpath wisc edu
    Science 309:1190-1. 2005
  32. ncbi request reprint Education. Scientific teaching
    Jo Handelsman
    Howard Hughes Medical Institute Professor, Department of Plant Pathology, University of Wisconsin Madison, Madison, Wisconsin, USA
    Science 304:521-2. 2004
  33. pmc Isolation of antibiotics turbomycin a and B from a metagenomic library of soil microbial DNA
    Doreen E Gillespie
    Department of Plant Pathology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Appl Environ Microbiol 68:4301-6. 2002
    ....
  34. ncbi request reprint Effect of clonal variation among hybrid poplars on susceptibility of gypsy moth (Lepidoptera: Lymantriidae) to Bacillus thuringiensis subsp. kurstaki
    Nichole A Broderick
    Department of Entomology, University of Wisconsin Madison, 1630 Linden Dr, Madison, WI 53706, USA
    J Econ Entomol 103:718-25. 2010
    ..thuringiensis, suggesting this interaction is more complex than merely reflecting higher mortality to previously stressed larvae...
  35. doi request reprint Call of the wild: antibiotic resistance genes in natural environments
    Heather K Allen
    Departments of Bacteriology and Plant Pathology, University of Wisconsin Madison, WI 53706, USA
    Nat Rev Microbiol 8:251-9. 2010
    ..This Review explores the presence and spread of antibiotic resistance in non-agricultural, non-clinical environments and demonstrates the need for more intensive investigation on this subject...
  36. pmc Use of a promoter trap to identify Bacillus cereus genes regulated by tomato seed exudate and a rhizosphere resident, Pseudomonas aureofaciens
    Anne K Dunn
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Appl Environ Microbiol 69:1197-205. 2003
    ..This study demonstrates the power of a promoter trap to identify genes in a gram-positive bacterium that are regulated by the biotic environment and resulted in the discovery of lipA, a plant-regulated gene in B. cereus...
  37. pmc Genetics of zwittermicin a production by Bacillus cereus
    Elizabeth A B Emmert
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Appl Environ Microbiol 70:104-13. 2004
    ..Additionally, nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) homologs are present, suggesting that zwittermicin A is synthesized by a mixed NRPS/PKS pathway...
  38. ncbi request reprint Does combination antimicrobial therapy reduce mortality in Gram-negative bacteraemia? A meta-analysis
    Nasia Safdar
    Section of Infectious Diseases, Department of Medicine, University of Wisconsin Medical School, Madison, USA
    Lancet Infect Dis 4:519-27. 2004
    ....
  39. pmc Toward a census of bacteria in soil
    Patrick D Schloss
    Department of Plant Pathology, University of Wisconsin Madison, Madison, Wisconsin, USA
    PLoS Comput Biol 2:e92. 2006
    ..We have shown that generating sufficient sequence data to do so requires less sequencing effort than completely sequencing a bacterial genome...
  40. pmc Integration of microbial ecology and statistics: a test to compare gene libraries
    Patrick D Schloss
    Department of Plant Pathology, University of Wisconsin Madison, 1630 Linden Dr, Madison, WI 53706, USA
    Appl Environ Microbiol 70:5485-92. 2004
    ....
  41. ncbi request reprint Thirteen-lined ground squirrels (Spermophilus tridecemlineatus) harbor multiantibiotic-resistant bacteria
    Karen A Cloud-Hansen
    Department of Plant Pathology, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA
    J Am Assoc Lab Anim Sci 46:21-3. 2007
    ..These findings support further study of antibiotic-resistant bacterial populations in wild-caught mammals used as laboratory models...
  42. ncbi request reprint The last word: books as a statistical metaphor for microbial communities
    Patrick D Schloss
    Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Annu Rev Microbiol 61:23-34. 2007
    ..Combined with other models that have been used to make inaccessible problems tractable, our book model offers a unique approach to the complex problem of describing microbial diversity...
  43. ncbi request reprint Identification and characterization of the gene encoding the Acidobacterium capsulatum major sigma factor
    Zakee L Sabree
    Department of Plant Pathology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Gene 376:144-51. 2006
    ..coli strain CAG20153 under conditions that prevent expression of the endogenous rpoD. These results indicate we have cloned the gene encoding the A. capsulatum major sigma factor and the gene product is active in E. coli...
  44. doi request reprint Psychrotrophic strain of Janthinobacterium lividum from a cold Alaskan soil produces prodigiosin
    Patrick D Schloss
    Departments of Bacteriology and Plant Pathology, University of Wisconsin, Madison, Wisconsin, USA
    DNA Cell Biol 29:533-41. 2010
    ..The results show that characterization of cultured organisms from previously unexplored environments can expand the current portrait of the microbial world...