F Hamra

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
    F Kent Hamra
    The Cecil H and Ida Green Center for Reproductive Biology Sciences, Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9051, USA
    Proc Natl Acad Sci U S A 102:17430-5. 2005
  2. ncbi Identification of neuregulin as a factor required for formation of aligned spermatogonia
    F Kent Hamra
    Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 282:721-30. 2007
  3. ncbi Spermatogonial culture medium: an effective and efficient nutrient mixture for culturing rat spermatogonial stem cells
    Zhuoru Wu
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center in Dallas, Dallas, Texas 75390, USA
    Biol Reprod 81:77-86. 2009
  4. ncbi Deriving mouse spermatogonial stem cell lines
    Ilaria Falciatori
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 450:181-92. 2008
  5. ncbi Sohlh2 knockout mice are male-sterile because of degeneration of differentiating type A spermatogonia
    Jing Hao
    Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Stem Cells 26:1587-97. 2008
  6. ncbi Defining the spermatogonial stem cell
    F Kent Hamra
    Cecil H and H Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Dev Biol 269:393-410. 2004
  7. ncbi Sterile testis complementation with spermatogonial lines restores fertility to DAZL-deficient rats and maximizes donor germline transmission
    Timothy E Richardson
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    PLoS ONE 4:e6308. 2009
  8. ncbi Isolating highly pure rat spermatogonial stem cells in culture
    F Kent Hamra
    Department of Pharmacology and Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 450:163-79. 2008
  9. ncbi Production of transgenic rats by lentiviral transduction of male germ-line stem cells
    F Kent Hamra
    Cecil H. and Ida Green Center for Reproductive Biology Sciences, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 99:14931-6. 2002
  10. ncbi Mutation of beta-glucosidase 2 causes glycolipid storage disease and impaired male fertility
    Yildiz Yildiz
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Clin Invest 116:2985-94. 2006

Research Grants

  1. Gene Targeting in Rat Spermatogonial Stem Cells
    F Hamra; Fiscal Year: 2007
  2. DISCOVERY & FUNCTION OF FERTILIZATION-SPECIFIC MOLECULES
    F Hamra; Fiscal Year: 2009
  3. Molecular Mechanisms of Spermatogonial Development
    F Hamra; Fiscal Year: 2009
  4. Biology of the ErbB Gene Family in Spermatogonial Development
    F Hamra; Fiscal Year: 2009
  5. Molecular Mechanisms of Spermatogonial Development
    F Kent Hamra; Fiscal Year: 2010
  6. Biology of the ErbB Gene Family in Spermatogonial Development
    F Kent Hamra; Fiscal Year: 2010

Collaborators

  • Zhuoru Wu
  • E N Olson
  • Guang Quan Zhao
  • Timothy E Richardson
  • Robert E Hammer
  • Robert J A Frost
  • Karen M Chapman
  • Ilaria Falciatori
  • Jing Hao
  • Yildiz Yildiz
  • David L Garbers
  • Nikolaus Schultz
  • Rhonda Bassel-Duby
  • Xiaoxia Qi
  • James A Richardson
  • Christina Tenenhaus Dann
  • Kate Lillard-Wetherell
  • Bray S Denard
  • Miwako Yamamoto
  • Rebekkah L Warren
  • David W Russell
  • Heidrun Matern
  • Jeremy C Allegood
  • Denise M O Ramirez
  • Siegfried Matern
  • Bonne Thompson

Detail Information

Publications12

  1. ncbi Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
    F Kent Hamra
    The Cecil H and Ida Green Center for Reproductive Biology Sciences, Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9051, USA
    Proc Natl Acad Sci U S A 102:17430-5. 2005
    ....
  2. ncbi Identification of neuregulin as a factor required for formation of aligned spermatogonia
    F Kent Hamra
    Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 282:721-30. 2007
    ..In fact, SNL cells were found to express 100-fold higher levels of neuregulin-1 transcripts than MSC-1 cells. Thus, we identify neuregulin as a factor required for spermatogonial amplification and differentiation in culture...
  3. ncbi Spermatogonial culture medium: an effective and efficient nutrient mixture for culturing rat spermatogonial stem cells
    Zhuoru Wu
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center in Dallas, Dallas, Texas 75390, USA
    Biol Reprod 81:77-86. 2009
    ..Spermatogonial lines derived and propagated using SG medium were characterized as homogeneous populations of ZBTB16(+) DAZL(+) cells endowed with spermatogonial stem cell potential...
  4. ncbi Deriving mouse spermatogonial stem cell lines
    Ilaria Falciatori
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 450:181-92. 2008
    ..We describe a simple and reproducible protocol for the derivation and maintenance of mouse spermatogonial stem cell lines that proliferate for long periods of time in culture...
  5. ncbi Sohlh2 knockout mice are male-sterile because of degeneration of differentiating type A spermatogonia
    Jing Hao
    Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Stem Cells 26:1587-97. 2008
    ..This regulation would provide a crucial checkpoint to optimize the numbers of spermatocytes entering meiosis during each cycle of spermatogenesis. Disclosure of potential conflicts of interest is found at the end of this article...
  6. ncbi Defining the spermatogonial stem cell
    F Kent Hamra
    Cecil H and H Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Dev Biol 269:393-410. 2004
    ..Thus, molecular markers specific for spermatogonial stem cells establish a reliable and rapid means by which to define these cells in culture and alleviate the need for laborious testicular transfers in initial cell culture studies...
  7. ncbi Sterile testis complementation with spermatogonial lines restores fertility to DAZL-deficient rats and maximizes donor germline transmission
    Timothy E Richardson
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    PLoS ONE 4:e6308. 2009
    ....
  8. ncbi Isolating highly pure rat spermatogonial stem cells in culture
    F Kent Hamra
    Department of Pharmacology and Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 450:163-79. 2008
    ..The isolated spermatogonia provide a highly potent and effective source of stem cells that have been used to initiate in vitro and in vivo culture studies on spermatogenesis...
  9. ncbi Production of transgenic rats by lentiviral transduction of male germ-line stem cells
    F Kent Hamra
    Cecil H. and Ida Green Center for Reproductive Biology Sciences, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 99:14931-6. 2002
    ..Thus, by using these procedures, approximately 30% of pups in the F(1) generation inherited and stably transmitted a lentiviral transgene that integrated at various genomic sites...
  10. ncbi Mutation of beta-glucosidase 2 causes glycolipid storage disease and impaired male fertility
    Yildiz Yildiz
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Clin Invest 116:2985-94. 2006
    ..We conclude that GBA2 is a glucosylceramidase whose loss causes accumulation of glycolipids and an endoplasmic reticulum storage disease...
  11. ncbi A multitude of genes expressed solely in meiotic or postmeiotic spermatogenic cells offers a myriad of contraceptive targets
    Nikolaus Schultz
    Cecil H. and Ida Green Center for Reproductive Biology Sciences, Howard Hughes Medical Institute and Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 100:12201-6. 2003
    ..This extensive group provides a plethora of potential targets for germ cell-directed contraception and a staggering number of candidate proteins that could be critical for fertilization...
  12. ncbi MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs
    Robert J A Frost
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Proc Natl Acad Sci U S A 107:11847-52. 2010
    ....

Research Grants8

  1. Gene Targeting in Rat Spermatogonial Stem Cells
    F Hamra; Fiscal Year: 2007
    ....
  2. DISCOVERY & FUNCTION OF FERTILIZATION-SPECIFIC MOLECULES
    F Hamra; Fiscal Year: 2009
    ..Based on these studies, linkage maps will begin to connect pathways from the sNHE, or other candidate gene products, to sperm behavioral responses such as motility activation or induction of the acrosome reaction. ..
  3. Molecular Mechanisms of Spermatogonial Development
    F Hamra; Fiscal Year: 2009
    ..abstract_text> ..
  4. Biology of the ErbB Gene Family in Spermatogonial Development
    F Hamra; Fiscal Year: 2009
    ..Thus, each aim focuses on progressing toward understanding the functional impact of ErbB3 to trigger a novel sub-set of spermatogonial stem cells to commence through the earliest steps in spermatogenesis ..
  5. Molecular Mechanisms of Spermatogonial Development
    F Kent Hamra; Fiscal Year: 2010
    ..abstract_text> ..
  6. Biology of the ErbB Gene Family in Spermatogonial Development
    F Kent Hamra; Fiscal Year: 2010
    ..Thus, each aim focuses on progressing toward understanding the functional impact of ErbB3 to trigger a novel sub-set of spermatogonial stem cells to commence through the earliest steps in spermatogenesis ..