Research Topics
| Christiane S HampeSummaryAffiliation: University of Washington Country: USA Publications
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Publications
Protective role of anti-idiotypic antibodies in autoimmunity--lessons for type 1 diabetesChristiane S Hampe
Department of Medicine, University of Washington, Seattle, WA 98109, USA
Autoimmunity 45:320-31. 2012..Finally, we conclude this review with a model of the events that may cause loss of anti-idiotypic antibodies and the implications for the development of T1D...
Species-specific autoantibodies in type 1 diabetesC S Hampe
Department of Medicine, University of Washington, Seattle 98195, USA
J Clin Endocrinol Metab 84:643-8. 1999..0002). This differentiation was not observed in 88 GAD65Ab-positive control subjects. Our data indicate that recognition of epitopes by GAD65Ab in type 1 diabetes is different from that in nontype 1 diabetes, GAD65Ab-positive individuals...
Longitudinal changes in epitope recognition of autoantibodies against glutamate decarboxylase 65 (GAD65Ab) in prediabetic adults developing diabetesC S Hampe
Department of Medicine, University of Washington, Seattle, WA, USA
Clin Exp Immunol 148:72-8. 2007....- Association of amino-terminal-specific antiglutamate decarboxylase (GAD65) autoantibodies with beta-cell functional reserve and a milder clinical phenotype in patients with GAD65 antibodies and ketosis-prone diabetes mellitusChristiane S Hampe
Robert H Williams Laboratory, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
J Clin Endocrinol Metab 92:462-7. 2007..The A+B- KPDM subgroup comprises patients with classic, autoimmune type 1 diabetes, whereas the A+B+ KPDM subgroup has only partial beta-cell loss and a distinct clinical phenotype... - Stable GAD65 autoantibody epitope patterns in type 1 diabetes children five years after onsetChristiane S Hampe
Robert H Williams Laboratory, Department of Medicine, Box 357710, University of Washington, Seattle 98195 7710, USA
J Autoimmun 18:49-53. 2002..The decrease in GAD65Ab levels did not correlate to residual C-peptide levels. Our data suggest that processes controlling GAD65Ab levels and epitope binding patterns remain stable during the first five years of type 1 diabetes... - Site-directed mutagenesis of K396R of the 65 kDa glutamic acid decarboxylase active site obliterates enzyme activity but not antibody bindingC S Hampe
Department of Medicine, University of Washington, Seattle 98195, USA
FEBS Lett 488:185-9. 2001.... - A novel monoclonal antibody specific for the N-terminal end of GAD65C S Hampe
Robert H Williams Laboratory, Department of Medicine, Box 357710, University of Washington, Seattle, 98105 7710, USA
J Neuroimmunol 113:63-71. 2001..Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously... - Recognition of glutamic acid decarboxylase (GAD) by autoantibodies from different GAD antibody-positive phenotypesC S Hampe
Department of Medicine, University of Washington, Seattle, Washington 98195, USA
J Clin Endocrinol Metab 85:4671-9. 2000..Our analysis demonstrates that the N-terminal part is essential for full antibody binding to GAD65, in particular, to the middle epitope. It is suggested that Type 1 diabetes is associated with restricted GAD65Ab epitope specificity... - Engineered antibodies for type 1 diabetesChristiane S Hampe
University of Washington, Department of Medicine, 815 Mercer Street, Seattle, WA 98109, USA
Curr Opin Investig Drugs 10:336-45. 2009..In this review, different engineering approaches and applications of engineered mAbs in the treatment of T1D are discussed... - Epitope analysis of GAD65Ab using fusion proteins and rFabKatherine A Binder
Department of Medicine, Box 357710 University of Washington, Seattle, WA 98195, USA
J Immunol Methods 295:101-9. 2004..A combined analysis using both approaches may therefore be necessary to gain best understanding of autoantibody characteristics and affinity maturation... - Ketosis-prone diabetes: dissection of a heterogeneous syndrome using an immunogenetic and beta-cell functional classification, prospective analysis, and clinical outcomesMario Maldonado
Division of Endocrinology, Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA
J Clin Endocrinol Metab 88:5090-8. 2003..Novel, nonautoimmune causes of beta-cell dysfunction are likely to underlie the A-beta+ and A-beta- syndromes... - The lack of anti-idiotypic antibodies, not the presence of the corresponding autoantibodies to glutamate decarboxylase, defines type 1 diabetesShilpa Oak
Department of Medicine, University of Washington, HSB 165, 1959 Pacific Street NE, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 105:5471-6. 2008..The lack of these inhibitory antibodies may contribute to T cell activation by GAD65Ab... - Recombinant Fabs of human monoclonal antibodies specific to the middle epitope of GAD65 inhibit type 1 diabetes-specific GAD65AbsCarolyn J Padoa
Department of Medicine, University of Washington, Seattle 98195, USA
Diabetes 52:2689-95. 2003..These GAD65-specific rFabs should prove useful in predicting type 1 diabetes and in the study of conformational GAD65Ab epitopes... - Comparison of three assays for the detection of GAD65Ab-specific anti-idiotypic antibodiesShilpa Oak
Department of Medicine, University of Washington, Building SLU II, 815 Mercer Street, Seattle, WA 98019, USA
J Immunol Methods 351:55-61. 2009.... - Development of glutamic acid decarboxylase 65 (GAD65) autoantibody assay using biotin-GAD65 fusion proteinDong Luo
Department of Medicine, University of Washington, P.O. Box 357710, HSB K-165, 1959 Pacific Street N.E, Seattle, WA 98195-7710, USA
J Biotechnol 111:97-104. 2004.... - Modulation of diabetes in NOD mice by GAD65-specific monoclonal antibodies is epitope specific and accompanied by anti-idiotypic antibodiesTyler R Hall
Department of Medicine, University of Washington, Seattle, WA 98195, USA
Immunology 123:547-54. 2008..11 in the anti-idiotypic antibody, supporting an immunomodulatory role for GAD65-specific autoantibodies, as originally postulated by Jerne... - Animal insulin therapy induces a biased insulin antibody response that persists for years after introduction of human insulinShilpa Oak
Department of Medicine, University of Washington, Seattle, WA, USA
Acta Diabetol 47:131-5. 2010..0001). We conclude that IA binding level and epitope specificity are biased by treatment with animal insulin. This bias remains for over 20 years after animal insulin treatment is terminated... - Antibodies to GAD65 and peripheral nerve function in the DCCTRobert D Hoeldtke
Department of Medicine, West Virginia University, Morgantown, WV 26506 9159, USA
J Neuroimmunol 185:182-9. 2007..248+/-.03 versus .278+/-.03). Epitope analysis, based on the blocking of conformational epitopes by recombinant Fab, revealed that the binding to multiple epitopes was decreased in the patients with neuropathy... - Immune reactivity to GAD25 in type 1 diabetes mellitusSteven D Chessler
Robert H Williams Laboratory, Department of Medicine, HSB K 161, Box 357710, University of Washington, Seattle, WA 98195 7710, USA
Autoimmunity 35:335-41. 2002..Our results indicate that autoreactivity to GAD25 is rare in newly diagnosed type 1 diabetes and does not underlie GAD67 reactivity... - Multiplicity of the antibody response to GAD65 in Type I diabetesL K Gilliam
Robert H. Williams Laboratory, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
Clin Exp Immunol 138:337-41. 2004..A significantly weaker competition was observed when evaluating sera of GAD65Ab-positive first-degree relatives (P < 0.0001)... - Conformation-dependent GAD65 autoantibodies in diabetesD Luo
Department of Medicine, University of Washington, R H Williams Laboratory, 1959 N E Pacific Street, Box 357710, Seattle, WA 98195 7710, USA
Diabetologia 47:1581-91. 2004..In this study we aimed to determine whether the substitution of GAD65 with GAD67 amino acids would affect the binding of conformation-dependent GAD65 autoantibodies... - Autoantibodies in diabetesCatherine Pihoker
Department of Pediatrics, University of Washington, Seattle, Washington 98195, USA
Diabetes 54:S52-61. 2005..Autoantibodies may also provide prognostic information in clinically heterogeneous patient populations when examined longitudinally... - Longitudinal epitope analysis of insulin-binding antibodies in type 1 diabetesT R Hall
Department of Medicine, University of Washington, Seattle, WA 98195, USA
Clin Exp Immunol 146:9-14. 2006..Using this approach we observed that insulin treatment is accompanied by a change in epitope specificities in the emerging IA... - Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assayDong Luo
Department of Medicine, University of Washington, Box 357710, HSB K-165, 1959 Pacific Street N.E, Seattle, WA 98195-7710, USA
J Biotechnol 103:249-55. 2003..We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 antibody... - Antigen presentation of detergent-free glutamate decarboxylase (GAD65) is affected by human serum albumin as carrier proteinJordan Steed
Robert H Williams Laboratory, Department of Medicine, University of Washington, Health Sciences Building K 165, 1959 Pacific Avenue NE, Seattle, WA 98195 3771, USA
J Immunol Methods 334:114-21. 2008..This suggests that HSA-GAD65 interactions qualitatively affect the epitope specificity of GAD65 presentation. HSA may enhance the 274-286 epitope presentation, while suppressing the 115-127 epitope... - Glutamate decarboxylase (GAD) autoantibody epitope shift during the first year of type 1 diabetesC S Hampe
Department of Medicine, University of Washington, Seattle 98195, USA
Horm Metab Res 31:553-7. 1999..Progression towards Type 1 diabetes is, however, associated with a maturation of the immune response towards human GAD65 autoreactivity... - Is latent autoimmune diabetes in adults distinct from type 1 diabetes or just type 1 diabetes at an older age?Jerry P Palmer
Department of Medicine, Division of Metabolism, Endocrinology and Nutrition, University of Washington, Seattle, USA
Diabetes 54:S62-7. 2005..These patients appear to be similar to type 1.5 patients in having decreased stimulated C-peptide values. These immunological differences between type 1 and type 1.5 diabetes suggest at least partially distinct disease processes... - GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccinationL M Bekris
Department of Medicine, University of Washington, Seattle, WA 98195, USA
Diabet Med 24:521-6. 2007..In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response... - Preservation of enzyme activity and antigenicity after mutagenesis of the membrane anchoring domain of GAD65A Plesner
Department of Medicine, University of Washington, Seattle 98195 7710, USA
Autoimmunity 34:221-30. 2001..Taken together, these data suggest that no major conformational changes have been introduced by mutating the membrane-anchoring domain of GAD65... - Respective implications of glutamate decarboxylase antibodies in stiff person syndrome and cerebellar ataxiaMario U Manto
Department of Medicine, Division of Metabolism, Endocrinology and Nutrition, University of Washington, Seattle, USA
Orphanet J Rare Dis 6:3. 2011..To investigate whether Stiff-person syndrome (SPS) and cerebellar ataxia (CA) are associated with distinct GAD65-Ab epitope specificities and neuronal effects... - Quantitative evaluation of a monoclonal antibody and its fragment as potential markers for pancreatic beta cell massC S Hampe
Department of Medicine, University of Washington, 1959 Pacific Street NE, Seattle, WA 98195, USA
Exp Clin Endocrinol Diabetes 113:381-7. 2005..Thus, generation of Fabs from IgGs with high beta cell binding and blood clearance appears feasible, but in order for molecules to be useful for tracking beta cell mass, antibodies of greater cellular specificity will have to be used... - GAD65 autoantibody responses in Japanese latent autoimmune diabetes in adult patientsTaro Maruyama
Department of Internal Medicine, Saitama Social Insurance Hospital, Saitama, Japan
Diabetes Care 31:1602-7. 2008..To determine whether development of insulin requirement in patients with latent autoimmune diabetes in adults (LADA) is accompanied with the emergence of a type 1 diabetes-like autoimmune response... - HLA class II alleles specify phenotypes of ketosis-prone diabetesRamaswami Nalini
Translational Metabolism Unit, Division of Endocrinology, Diabetes, and Metabolism, Baylor College of Medicine, Houston, Texas 77030, USA
Diabetes Care 31:1195-200. 2008..Genetic factors could contribute to their distinctive phenotypes. Our aim was to specify the role of HLA class II alleles associated with susceptibility or resistance to autoimmune type 1 diabetes in determining KPD phenotypes... - Immunobiology of stiff-person syndromeRaghavan Raju
Department of Surgery, University of Alabama at Birmingham, Birmingham, Alabama 35294 0019, USA
Int Rev Immunol 27:79-92. 2008..Clin. Neuropathol. 16: 214-219, 1997; Ishizawa et al., Acta Neuropathol.(Berl) 97: 63-70, 1999]. This review discusses the possible role of autoantibodies and autoreactive T cells specific to neuronal antigens in SPS pathogenesis... - Analysis of GAD65 autoantibodies in Stiff-Person syndrome patientsRaghavanpillai Raju
Neuromuscular Diseases Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 175:7755-62. 2005..A mAb (N-GAD65 mAb) recognizing this N-terminal epitope was successfully humanized to enhance its potential therapeutic value by reducing its overall immunogenicity... - COOH-terminal clustering of autoantibody and T-cell determinants on the structure of GAD65 provide insights into the molecular basis of autoreactivityGustavo Fenalti
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Diabetes 57:1293-301. 2008.... - GAD65 antibody epitope patterns of type 1.5 diabetic patients are consistent with slow-onset autoimmune diabetesChristiane S Hampe
Diabetes Care 25:1481-2. 2002 - Species and epitope specificity of two 65 kDa glutamate decarboxylase time-resolved fluorometric immunoassaysMao Rui
Diabetes Research Center, Brussels Free University, Brussels, Belgium
J Immunol Methods 319:133-43. 2007..We show that the beta cell-specific marker GAD65 can be quantified in pancreatic cell extracts and in serum, allowing studies on discharge during cell death in vitro as well as in vivo... - Molecular characterization of a disease associated conformational epitope on GAD65 recognised by a human monoclonal antibody b96.11Gustavo Fenalti
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Mol Immunol 44:1178-89. 2007..The study shows the utility of using a combination of in silico techniques and experimental data for molecular characterization and localization of conformational epitopes for which crystal structures are lacking... - Development of type 1 diabetes in wild bank voles associated with islet autoantibodies and the novel ljungan virusBo Niklasson
Apodemus AB, Stockholm, Sweden
Int J Exp Diabesity Res 4:35-44. 2003..It is speculated that bank voles may have a possible zoonotic role as a reservoir and vector for virus that may contribute to the incidence of type 1 diabetes in humans... - GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loopGustavo Fenalti
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne, VIC 3800, Australia
Nat Struct Mol Biol 14:280-6. 2007..Kinetic studies suggest that mobility in the catalytic loop promotes a side reaction that results in cofactor release and GAD65 autoinactivation. These data reveal the molecular basis for regulation of GABA homeostasis... - Epitope-restricted 65-kilodalton glutamic acid decarboxylase autoantibodies among new-onset Sardinian type 2 diabetes patients define phenotypes of autoimmune diabetesMario Maioli
Metabolic Disease Unit, Istituto di Clinica Medica, University of Sassari, Italy
J Clin Endocrinol Metab 89:5675-82. 2004..Thus, GAD65Ab epitope analysis may help to define T1DM phenotypes among newly diagnosed GAD65Ab-positive patients classified with T2DM... - Seasonality of month of birth differs between type 1 diabetes patients with pronounced beta-cell autoimmunity and individuals with lesser or no beta-cell autoimmunityHadas Lewy
Schneider Children s Medical Center of Israel, Petah Tikva, Israel
Pediatr Diabetes 9:46-52. 2008.... - Accuracy and predictive value of classification schemes for ketosis-prone diabetesAshok Balasubramanyam
Translational Metabolism Unit, Baylor College of Medicine, One Baylor Plaza, Room 520 N, Houston, TX 77030, USA
Diabetes Care 29:2575-9. 2006..972. CONCLUSIONS: The Abeta scheme has the highest accuracy and predictive value in classifying KPD patients with regard to clinical outcomes and pathophysiologic subtypes... - Syndromes of ketosis-prone diabetes mellitusAshok Balasubramanyam
Translational Metabolism Unit, Division of Diabetes, Endocrinology and Metabolism, Baylor College of Medicine, Room 700B, One Baylor Plaza, and Endocrine Service, Ben Taub General Hospital, Houston, Texas 77030, USA
Endocr Rev 29:292-302. 2008....
Research Grants
- ISLET CELL MEMBRANE ANTIBODIES IN DIABETESChristiane S Hampe; Fiscal Year: 2010..This research aims to uncover the mechanisms of the early immune response to GAD65 and the relationship between the autoantibody response maturation and progression to clinical onset of T1D. ..