Christiane S Hampe

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. doi request reprint High titers of autoantibodies to glutamate decarboxylase in type 1 diabetes patients: epitope analysis and inhibition of enzyme activity
    Christiane S Hampe
    Department of Medicine, University of Washington, Seattle, Washington 98109, USA
    Endocr Pract 19:663-8. 2013
  2. pmc Monoclonal antibodies to 65kDa glutamate decarboxylase induce epitope specific effects on motor and cognitive functions in rats
    Christiane S Hampe
    University of Washington, School of Medicine, SLU S 276, Seattle, WA 98109, USA
    Orphanet J Rare Dis 8:82. 2013
  3. doi request reprint Protective role of anti-idiotypic antibodies in autoimmunity--lessons for type 1 diabetes
    Christiane S Hampe
    Department of Medicine, University of Washington, Seattle, WA 98109, USA
    Autoimmunity 45:320-31. 2012
  4. pmc Longitudinal changes in epitope recognition of autoantibodies against glutamate decarboxylase 65 (GAD65Ab) in prediabetic adults developing diabetes
    C S Hampe
    Department of Medicine, University of Washington, Seattle, WA, USA
    Clin Exp Immunol 148:72-8. 2007
  5. ncbi request reprint Engineered antibodies for type 1 diabetes
    Christiane S Hampe
    University of Washington, Department of Medicine, 815 Mercer Street, Seattle, WA 98109, USA
    Curr Opin Investig Drugs 10:336-45. 2009
  6. ncbi request reprint Association of amino-terminal-specific antiglutamate decarboxylase (GAD65) autoantibodies with beta-cell functional reserve and a milder clinical phenotype in patients with GAD65 antibodies and ketosis-prone diabetes mellitus
    Christiane S Hampe
    Robert H Williams Laboratory, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Clin Endocrinol Metab 92:462-7. 2007
  7. ncbi request reprint Stable GAD65 autoantibody epitope patterns in type 1 diabetes children five years after onset
    Christiane S Hampe
    Robert H Williams Laboratory, Department of Medicine, Box 357710, University of Washington, Seattle 98195 7710, USA
    J Autoimmun 18:49-53. 2002
  8. ncbi request reprint Site-directed mutagenesis of K396R of the 65 kDa glutamic acid decarboxylase active site obliterates enzyme activity but not antibody binding
    C S Hampe
    Department of Medicine, University of Washington, Seattle 98195, USA
    FEBS Lett 488:185-9. 2001
  9. ncbi request reprint A novel monoclonal antibody specific for the N-terminal end of GAD65
    C S Hampe
    Robert H Williams Laboratory, Department of Medicine, Box 357710, University of Washington, Seattle, 98105 7710, USA
    J Neuroimmunol 113:63-71. 2001
  10. ncbi request reprint Recognition of glutamic acid decarboxylase (GAD) by autoantibodies from different GAD antibody-positive phenotypes
    C S Hampe
    Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Clin Endocrinol Metab 85:4671-9. 2000

Research Grants

  1. ISLET CELL MEMBRANE ANTIBODIES IN DIABETES
    Christiane S Hampe; Fiscal Year: 2010

Collaborators

Detail Information

Publications47

  1. doi request reprint High titers of autoantibodies to glutamate decarboxylase in type 1 diabetes patients: epitope analysis and inhibition of enzyme activity
    Christiane S Hampe
    Department of Medicine, University of Washington, Seattle, Washington 98109, USA
    Endocr Pract 19:663-8. 2013
    ..We hypothesized that the frequency of neurological disorders with high GAD65Ab titers is significantly higher than currently recognized...
  2. pmc Monoclonal antibodies to 65kDa glutamate decarboxylase induce epitope specific effects on motor and cognitive functions in rats
    Christiane S Hampe
    University of Washington, School of Medicine, SLU S 276, Seattle, WA 98109, USA
    Orphanet J Rare Dis 8:82. 2013
    ..To assess the effect of epitope-specific GAD65Ab on cognitive and motor functions, we conducted behavioral experiments in rats that received cerebellar injections with two distinct monoclonal GAD65Ab (b96.11 and b78)...
  3. doi request reprint Protective role of anti-idiotypic antibodies in autoimmunity--lessons for type 1 diabetes
    Christiane S Hampe
    Department of Medicine, University of Washington, Seattle, WA 98109, USA
    Autoimmunity 45:320-31. 2012
    ..Finally, we conclude this review with a model of the events that may cause loss of anti-idiotypic antibodies and the implications for the development of T1D...
  4. pmc Longitudinal changes in epitope recognition of autoantibodies against glutamate decarboxylase 65 (GAD65Ab) in prediabetic adults developing diabetes
    C S Hampe
    Department of Medicine, University of Washington, Seattle, WA, USA
    Clin Exp Immunol 148:72-8. 2007
    ....
  5. ncbi request reprint Engineered antibodies for type 1 diabetes
    Christiane S Hampe
    University of Washington, Department of Medicine, 815 Mercer Street, Seattle, WA 98109, USA
    Curr Opin Investig Drugs 10:336-45. 2009
    ..In this review, different engineering approaches and applications of engineered mAbs in the treatment of T1D are discussed...
  6. ncbi request reprint Association of amino-terminal-specific antiglutamate decarboxylase (GAD65) autoantibodies with beta-cell functional reserve and a milder clinical phenotype in patients with GAD65 antibodies and ketosis-prone diabetes mellitus
    Christiane S Hampe
    Robert H Williams Laboratory, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Clin Endocrinol Metab 92:462-7. 2007
    ..The A+B- KPDM subgroup comprises patients with classic, autoimmune type 1 diabetes, whereas the A+B+ KPDM subgroup has only partial beta-cell loss and a distinct clinical phenotype...
  7. ncbi request reprint Stable GAD65 autoantibody epitope patterns in type 1 diabetes children five years after onset
    Christiane S Hampe
    Robert H Williams Laboratory, Department of Medicine, Box 357710, University of Washington, Seattle 98195 7710, USA
    J Autoimmun 18:49-53. 2002
    ..The decrease in GAD65Ab levels did not correlate to residual C-peptide levels. Our data suggest that processes controlling GAD65Ab levels and epitope binding patterns remain stable during the first five years of type 1 diabetes...
  8. ncbi request reprint Site-directed mutagenesis of K396R of the 65 kDa glutamic acid decarboxylase active site obliterates enzyme activity but not antibody binding
    C S Hampe
    Department of Medicine, University of Washington, Seattle 98195, USA
    FEBS Lett 488:185-9. 2001
    ....
  9. ncbi request reprint A novel monoclonal antibody specific for the N-terminal end of GAD65
    C S Hampe
    Robert H Williams Laboratory, Department of Medicine, Box 357710, University of Washington, Seattle, 98105 7710, USA
    J Neuroimmunol 113:63-71. 2001
    ..Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously...
  10. ncbi request reprint Recognition of glutamic acid decarboxylase (GAD) by autoantibodies from different GAD antibody-positive phenotypes
    C S Hampe
    Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Clin Endocrinol Metab 85:4671-9. 2000
    ..Our analysis demonstrates that the N-terminal part is essential for full antibody binding to GAD65, in particular, to the middle epitope. It is suggested that Type 1 diabetes is associated with restricted GAD65Ab epitope specificity...
  11. ncbi request reprint Species-specific autoantibodies in type 1 diabetes
    C S Hampe
    Department of Medicine, University of Washington, Seattle 98195, USA
    J Clin Endocrinol Metab 84:643-8. 1999
    ..0002). This differentiation was not observed in 88 GAD65Ab-positive control subjects. Our data indicate that recognition of epitopes by GAD65Ab in type 1 diabetes is different from that in nontype 1 diabetes, GAD65Ab-positive individuals...
  12. ncbi request reprint Epitope analysis of GAD65Ab using fusion proteins and rFab
    Katherine A Binder
    Department of Medicine, Box 357710 University of Washington, Seattle, WA 98195, USA
    J Immunol Methods 295:101-9. 2004
    ..A combined analysis using both approaches may therefore be necessary to gain best understanding of autoantibody characteristics and affinity maturation...
  13. ncbi request reprint Ketosis-prone diabetes: dissection of a heterogeneous syndrome using an immunogenetic and beta-cell functional classification, prospective analysis, and clinical outcomes
    Mario Maldonado
    Division of Endocrinology, Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA
    J Clin Endocrinol Metab 88:5090-8. 2003
    ..Novel, nonautoimmune causes of beta-cell dysfunction are likely to underlie the A-beta+ and A-beta- syndromes...
  14. ncbi request reprint Development of glutamic acid decarboxylase 65 (GAD65) autoantibody assay using biotin-GAD65 fusion protein
    Dong Luo
    Department of Medicine, University of Washington, P O Box 357710, HSB K 165, 1959 Pacific Street N E, Seattle, WA 98195 7710, USA
    J Biotechnol 111:97-104. 2004
    ....
  15. pmc The lack of anti-idiotypic antibodies, not the presence of the corresponding autoantibodies to glutamate decarboxylase, defines type 1 diabetes
    Shilpa Oak
    Department of Medicine, University of Washington, HSB 165, 1959 Pacific Street NE, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 105:5471-6. 2008
    ..The lack of these inhibitory antibodies may contribute to T cell activation by GAD65Ab...
  16. pmc Modulation of diabetes in NOD mice by GAD65-specific monoclonal antibodies is epitope specific and accompanied by anti-idiotypic antibodies
    Tyler R Hall
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Immunology 123:547-54. 2008
    ..11 in the anti-idiotypic antibody, supporting an immunomodulatory role for GAD65-specific autoantibodies, as originally postulated by Jerne...
  17. pmc Comparison of three assays for the detection of GAD65Ab-specific anti-idiotypic antibodies
    Shilpa Oak
    Department of Medicine, University of Washington, Building SLU II, 815 Mercer Street, Seattle, WA 98019, USA
    J Immunol Methods 351:55-61. 2009
    ....
  18. doi request reprint Animal insulin therapy induces a biased insulin antibody response that persists for years after introduction of human insulin
    Shilpa Oak
    Department of Medicine, University of Washington, Seattle, WA, USA
    Acta Diabetol 47:131-5. 2010
    ..0001). We conclude that IA binding level and epitope specificity are biased by treatment with animal insulin. This bias remains for over 20 years after animal insulin treatment is terminated...
  19. ncbi request reprint Recombinant Fabs of human monoclonal antibodies specific to the middle epitope of GAD65 inhibit type 1 diabetes-specific GAD65Abs
    Carolyn J Padoa
    Department of Medicine, University of Washington, Seattle 98195, USA
    Diabetes 52:2689-95. 2003
    ..These GAD65-specific rFabs should prove useful in predicting type 1 diabetes and in the study of conformational GAD65Ab epitopes...
  20. ncbi request reprint Antibodies to GAD65 and peripheral nerve function in the DCCT
    Robert D Hoeldtke
    Department of Medicine, West Virginia University, Morgantown, WV 26506 9159, USA
    J Neuroimmunol 185:182-9. 2007
    ..248+/-.03 versus .278+/-.03). Epitope analysis, based on the blocking of conformational epitopes by recombinant Fab, revealed that the binding to multiple epitopes was decreased in the patients with neuropathy...
  21. ncbi request reprint Immune reactivity to GAD25 in type 1 diabetes mellitus
    Steven D Chessler
    Robert H Williams Laboratory, Department of Medicine, HSB K 161, Box 357710, University of Washington, Seattle, WA 98195 7710, USA
    Autoimmunity 35:335-41. 2002
    ..Our results indicate that autoreactivity to GAD25 is rare in newly diagnosed type 1 diabetes and does not underlie GAD67 reactivity...
  22. ncbi request reprint Conformation-dependent GAD65 autoantibodies in diabetes
    D Luo
    Department of Medicine, University of Washington, R H Williams Laboratory, 1959 N E Pacific Street, Box 357710, Seattle, WA 98195 7710, USA
    Diabetologia 47:1581-91. 2004
    ..In this study we aimed to determine whether the substitution of GAD65 with GAD67 amino acids would affect the binding of conformation-dependent GAD65 autoantibodies...
  23. pmc Multiplicity of the antibody response to GAD65 in Type I diabetes
    L K Gilliam
    Robert H Williams Laboratory, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Clin Exp Immunol 138:337-41. 2004
    ..A significantly weaker competition was observed when evaluating sera of GAD65Ab-positive first-degree relatives (P < 0.0001)...
  24. ncbi request reprint Autoantibodies in diabetes
    Catherine Pihoker
    Department of Pediatrics, University of Washington, Seattle, Washington 98195, USA
    Diabetes 54:S52-61. 2005
    ..Autoantibodies may also provide prognostic information in clinically heterogeneous patient populations when examined longitudinally...
  25. pmc Longitudinal epitope analysis of insulin-binding antibodies in type 1 diabetes
    T R Hall
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Clin Exp Immunol 146:9-14. 2006
    ..Using this approach we observed that insulin treatment is accompanied by a change in epitope specificities in the emerging IA...
  26. ncbi request reprint Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay
    Dong Luo
    Department of Medicine, University of Washington, Box 357710, HSB K 165, 1959 Pacific Street N E, Seattle, WA 98195 7710, USA
    J Biotechnol 103:249-55. 2003
    ..We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 antibody...
  27. pmc Antigen presentation of detergent-free glutamate decarboxylase (GAD65) is affected by human serum albumin as carrier protein
    Jordan Steed
    Robert H Williams Laboratory, Department of Medicine, University of Washington, Health Sciences Building K 165, 1959 Pacific Avenue NE, Seattle, WA 98195 3771, USA
    J Immunol Methods 334:114-21. 2008
    ..This suggests that HSA-GAD65 interactions qualitatively affect the epitope specificity of GAD65 presentation. HSA may enhance the 274-286 epitope presentation, while suppressing the 115-127 epitope...
  28. ncbi request reprint Glutamate decarboxylase (GAD) autoantibody epitope shift during the first year of type 1 diabetes
    C S Hampe
    Department of Medicine, University of Washington, Seattle 98195, USA
    Horm Metab Res 31:553-7. 1999
    ..Progression towards Type 1 diabetes is, however, associated with a maturation of the immune response towards human GAD65 autoreactivity...
  29. pmc Respective implications of glutamate decarboxylase antibodies in stiff person syndrome and cerebellar ataxia
    Mario U Manto
    Department of Medicine, Division of Metabolism, Endocrinology and Nutrition, University of Washington, Seattle, USA
    Orphanet J Rare Dis 6:3. 2011
    ..To investigate whether Stiff-person syndrome (SPS) and cerebellar ataxia (CA) are associated with distinct GAD65-Ab epitope specificities and neuronal effects...
  30. ncbi request reprint Is latent autoimmune diabetes in adults distinct from type 1 diabetes or just type 1 diabetes at an older age?
    Jerry P Palmer
    Department of Medicine, Division of Metabolism, Endocrinology and Nutrition, University of Washington, Seattle, USA
    Diabetes 54:S62-7. 2005
    ..These patients appear to be similar to type 1.5 patients in having decreased stimulated C-peptide values. These immunological differences between type 1 and type 1.5 diabetes suggest at least partially distinct disease processes...
  31. ncbi request reprint Preservation of enzyme activity and antigenicity after mutagenesis of the membrane anchoring domain of GAD65
    A Plesner
    Department of Medicine, University of Washington, Seattle 98195 7710, USA
    Autoimmunity 34:221-30. 2001
    ..Taken together, these data suggest that no major conformational changes have been introduced by mutating the membrane-anchoring domain of GAD65...
  32. ncbi request reprint GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination
    L M Bekris
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Diabet Med 24:521-6. 2007
    ..In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response...
  33. ncbi request reprint Quantitative evaluation of a monoclonal antibody and its fragment as potential markers for pancreatic beta cell mass
    C S Hampe
    Department of Medicine, University of Washington, 1959 Pacific Street NE, Seattle, WA 98195, USA
    Exp Clin Endocrinol Diabetes 113:381-7. 2005
    ..Thus, generation of Fabs from IgGs with high beta cell binding and blood clearance appears feasible, but in order for molecules to be useful for tracking beta cell mass, antibodies of greater cellular specificity will have to be used...
  34. ncbi request reprint GAD65 antibody epitope patterns of type 1.5 diabetic patients are consistent with slow-onset autoimmune diabetes
    Christiane S Hampe
    Diabetes Care 25:1481-2. 2002
  35. pmc GAD65 autoantibody responses in Japanese latent autoimmune diabetes in adult patients
    Taro Maruyama
    Department of Internal Medicine, Saitama Social Insurance Hospital, Saitama, Japan
    Diabetes Care 31:1602-7. 2008
    ..To determine whether development of insulin requirement in patients with latent autoimmune diabetes in adults (LADA) is accompanied with the emergence of a type 1 diabetes-like autoimmune response...
  36. pmc Development of type 1 diabetes in wild bank voles associated with islet autoantibodies and the novel ljungan virus
    Bo Niklasson
    Apodemus AB, Stockholm, Sweden
    Int J Exp Diabesity Res 4:35-44. 2003
    ..It is speculated that bank voles may have a possible zoonotic role as a reservoir and vector for virus that may contribute to the incidence of type 1 diabetes in humans...
  37. doi request reprint Immunobiology of stiff-person syndrome
    Raghavan Raju
    Department of Surgery, University of Alabama at Birmingham, Birmingham, Alabama 35294 0019, USA
    Int Rev Immunol 27:79-92. 2008
    ..Clin. Neuropathol. 16: 214-219, 1997; Ishizawa et al., Acta Neuropathol.(Berl) 97: 63-70, 1999]. This review discusses the possible role of autoantibodies and autoreactive T cells specific to neuronal antigens in SPS pathogenesis...
  38. ncbi request reprint Analysis of GAD65 autoantibodies in Stiff-Person syndrome patients
    Raghavanpillai Raju
    Neuromuscular Diseases Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 175:7755-62. 2005
    ..A mAb (N-GAD65 mAb) recognizing this N-terminal epitope was successfully humanized to enhance its potential therapeutic value by reducing its overall immunogenicity...
  39. ncbi request reprint Molecular characterization of a disease associated conformational epitope on GAD65 recognised by a human monoclonal antibody b96.11
    Gustavo Fenalti
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
    Mol Immunol 44:1178-89. 2007
    ..The study shows the utility of using a combination of in silico techniques and experimental data for molecular characterization and localization of conformational epitopes for which crystal structures are lacking...
  40. ncbi request reprint Epitope-restricted 65-kilodalton glutamic acid decarboxylase autoantibodies among new-onset Sardinian type 2 diabetes patients define phenotypes of autoimmune diabetes
    Mario Maioli
    Metabolic Disease Unit, Istituto di Clinica Medica, University of Sassari, Italy
    J Clin Endocrinol Metab 89:5675-82. 2004
    ..Thus, GAD65Ab epitope analysis may help to define T1DM phenotypes among newly diagnosed GAD65Ab-positive patients classified with T2DM...
  41. ncbi request reprint Species and epitope specificity of two 65 kDa glutamate decarboxylase time-resolved fluorometric immunoassays
    Mao Rui
    Diabetes Research Center, Brussels Free University, Brussels, Belgium
    J Immunol Methods 319:133-43. 2007
    ..We show that the beta cell-specific marker GAD65 can be quantified in pancreatic cell extracts and in serum, allowing studies on discharge during cell death in vitro as well as in vivo...
  42. ncbi request reprint GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop
    Gustavo Fenalti
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne, VIC 3800, Australia
    Nat Struct Mol Biol 14:280-6. 2007
    ..Kinetic studies suggest that mobility in the catalytic loop promotes a side reaction that results in cofactor release and GAD65 autoinactivation. These data reveal the molecular basis for regulation of GABA homeostasis...
  43. ncbi request reprint Seasonality of month of birth differs between type 1 diabetes patients with pronounced beta-cell autoimmunity and individuals with lesser or no beta-cell autoimmunity
    Hadas Lewy
    Schneider Children s Medical Center of Israel, Petah Tikva, Israel
    Pediatr Diabetes 9:46-52. 2008
    ....
  44. doi request reprint COOH-terminal clustering of autoantibody and T-cell determinants on the structure of GAD65 provide insights into the molecular basis of autoreactivity
    Gustavo Fenalti
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
    Diabetes 57:1293-301. 2008
    ....
  45. doi request reprint HLA class II alleles specify phenotypes of ketosis-prone diabetes
    Ramaswami Nalini
    Translational Metabolism Unit, Division of Endocrinology, Diabetes, and Metabolism, Baylor College of Medicine, Houston, Texas 77030, USA
    Diabetes Care 31:1195-200. 2008
    ..Genetic factors could contribute to their distinctive phenotypes. Our aim was to specify the role of HLA class II alleles associated with susceptibility or resistance to autoimmune type 1 diabetes in determining KPD phenotypes...
  46. ncbi request reprint Accuracy and predictive value of classification schemes for ketosis-prone diabetes
    Ashok Balasubramanyam
    Translational Metabolism Unit, Baylor College of Medicine, One Baylor Plaza, Room 520 N, Houston, TX 77030, USA
    Diabetes Care 29:2575-9. 2006
    ....
  47. pmc Syndromes of ketosis-prone diabetes mellitus
    Ashok Balasubramanyam
    Translational Metabolism Unit, Division of Diabetes, Endocrinology and Metabolism, Baylor College of Medicine, Room 700B, One Baylor Plaza, and Endocrine Service, Ben Taub General Hospital, Houston, Texas 77030, USA
    Endocr Rev 29:292-302. 2008
    ....

Research Grants2

  1. ISLET CELL MEMBRANE ANTIBODIES IN DIABETES
    Christiane S Hampe; Fiscal Year: 2010
    ..This research aims to uncover the mechanisms of the early immune response to GAD65 and the relationship between the autoantibody response maturation and progression to clinical onset of T1D. ..