Perry B Hackett

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. pmc Integrating DNA vectors for gene therapy
    Perry B Hackett
    Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 15:10-2. 2007
  2. pmc Gene expression in lung and liver after intravenous infusion of polyethylenimine complexes of Sleeping Beauty transposons
    Kelly M Podetz-Pedersen
    Beckman Center for Transposon Research, Center for Genome Engineering, Institute of Human Genetics, and Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Hum Gene Ther 21:210-20. 2010
  3. pmc Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon
    Aron M Geurts
    Department of Genetics, Cell Biology, and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    BMC Biotechnol 6:30. 2006
  4. pmc Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis
    Ying Wang
    Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA
    Development 137:3119-28. 2010
  5. ncbi request reprint RNA as a source of transposase for Sleeping Beauty-mediated gene insertion and expression in somatic cells and tissues
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:625-30. 2006
  6. ncbi request reprint Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression
    Paul R Score
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:617-24. 2006
  7. ncbi request reprint Sleeping beauty transposon-mediated gene therapy for prolonged expression
    Perry B Hackett
    Department of Genetics, Cell Biology and Development Arnold and Mabel Beckman Center for Transposon Research University of Minnesota Minneapolis, Minnesota 55455, USA
    Adv Genet 54:189-232. 2005
  8. pmc Efficacy and safety of Sleeping Beauty transposon-mediated gene transfer in preclinical animal studies
    Perry B Hackett
    Dept of Genetics, Cell Biology and Development, 321 Church St SE, Minneapolis, MN 55455, USA
    Curr Gene Ther 11:341-9. 2011
  9. pmc Systemic correction of storage disease in MPS I NOD/SCID mice using the sleeping beauty transposon system
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development, Center for Genome Engineering, University of Minnesota, Minneapolis, 55455, USA
    Mol Ther 17:1136-44. 2009
  10. doi request reprint Sleeping Beauty-mediated correction of Fanconi anemia type C
    Kendra A Hyland
    Discovery Genomics, Inc, Minneapolis, MN, USA
    J Gene Med 13:462-9. 2011

Research Grants

  1. SB Transposons for Gene Therapy
    Perry B Hackett; Fiscal Year: 2010

Collaborators

Detail Information

Publications38

  1. pmc Integrating DNA vectors for gene therapy
    Perry B Hackett
    Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 15:10-2. 2007
  2. pmc Gene expression in lung and liver after intravenous infusion of polyethylenimine complexes of Sleeping Beauty transposons
    Kelly M Podetz-Pedersen
    Beckman Center for Transposon Research, Center for Genome Engineering, Institute of Human Genetics, and Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Hum Gene Ther 21:210-20. 2010
    ..These results provide insight into the relative effectiveness and organ specificity of these two methods of nonviral gene delivery when coupled with the Sleeping Beauty transposon system...
  3. pmc Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon
    Aron M Geurts
    Department of Genetics, Cell Biology, and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    BMC Biotechnol 6:30. 2006
    ..We sought to generate a transposon system that would be capable of reporting the expression pattern of a mouse gene while allowing for conditional expression of a gene of interest in a tissue- or temporal-specific pattern...
  4. pmc Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis
    Ying Wang
    Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA
    Development 137:3119-28. 2010
    ..We suggest that Ve-cadherin and Moesin1 function to establish and maintain apical/basal polarity during multicellular lumen formation in the ISVs...
  5. ncbi request reprint RNA as a source of transposase for Sleeping Beauty-mediated gene insertion and expression in somatic cells and tissues
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:625-30. 2006
    ..We conclude that in vitro-transcribed mRNA can be used as an effective source of transposase for SB-mediated transposition in mammalian cells and tissues...
  6. ncbi request reprint Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression
    Paul R Score
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:617-24. 2006
    ..The results also provide additional evidence for the effectiveness of the Sleeping Beauty transposon system as an in vivo DNA-mediated gene transfer strategy for achieving long-term expression...
  7. ncbi request reprint Sleeping beauty transposon-mediated gene therapy for prolonged expression
    Perry B Hackett
    Department of Genetics, Cell Biology and Development Arnold and Mabel Beckman Center for Transposon Research University of Minnesota Minneapolis, Minnesota 55455, USA
    Adv Genet 54:189-232. 2005
    ..Here we review the activities of the SB system in mice used as a model for human gene therapy, methods of delivery of the SB system, and its efficacy in ameliorating disorders that model human disease...
  8. pmc Efficacy and safety of Sleeping Beauty transposon-mediated gene transfer in preclinical animal studies
    Perry B Hackett
    Dept of Genetics, Cell Biology and Development, 321 Church St SE, Minneapolis, MN 55455, USA
    Curr Gene Ther 11:341-9. 2011
    ..The ability to correct genetic diseases through the infusion of DNA plasmids remains an appealing goal...
  9. pmc Systemic correction of storage disease in MPS I NOD/SCID mice using the sleeping beauty transposon system
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development, Center for Genome Engineering, University of Minnesota, Minneapolis, 55455, USA
    Mol Ther 17:1136-44. 2009
    ....
  10. doi request reprint Sleeping Beauty-mediated correction of Fanconi anemia type C
    Kendra A Hyland
    Discovery Genomics, Inc, Minneapolis, MN, USA
    J Gene Med 13:462-9. 2011
    ..Our goal is to develop the SB system for nonviral complementation of Fanconi anemia (FA), a rare autosomal recessive disorder accompanied by progressive bone marrow failure...
  11. pmc Harnessing a high cargo-capacity transposon for genetic applications in vertebrates
    Darius Balciunas
    The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America
    PLoS Genet 2:e169. 2006
    ..Together, Tol2 provides a novel nonviral vector for the delivery of large genetic payloads for gene therapy and other transgenic applications...
  12. pmc Prolonged expression of a lysosomal enzyme in mouse liver after Sleeping Beauty transposon-mediated gene delivery: implications for non-viral gene therapy of mucopolysaccharidoses
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    J Gene Med 9:403-15. 2007
    ..The Sleeping Beauty (SB) transposon system is a non-viral vector system that can integrate precise sequences into chromosomes. We evaluated the SB transposon system as a tool for gene therapy of mucopolysaccharidosis (MPS) types I and VII...
  13. ncbi request reprint Counterselection and co-delivery of transposon and transposase functions for Sleeping Beauty-mediated transposition in cultured mammalian cells
    Andrea D Converse
    Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, 6 160 Jackson Hall 321 Church Street S E, Minneapolis, MN, 55455, USA
    Biosci Rep 24:577-94. 2004
    ....
  14. pmc Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection
    Jason B Bell
    Department of Genetics, Cell Biology and Development, Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, 6 160 Jackson Hall, 321 Church Street SE, Minneapolis, Minnesota 55455, USA
    Nat Protoc 2:3153-65. 2007
    ..Several weeks after injection, transgene expression stabilizes at approximately 1% of the level at 24 h, presumably owing to integration of the transposons into chromosomes...
  15. pmc Duration of expression and activity of Sleeping Beauty transposase in mouse liver following hydrodynamic DNA delivery
    Jason B Bell
    Department of Genetics, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 18:1796-802. 2010
    ..Thus, within the limits of current technology, we show that SB transposons appear to be as stably integrated as their viral counterparts...
  16. pmc The Sleeping Beauty transposon system: a non-viral vector for gene therapy
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development, The Center for Genome Engineering, Institute of Human Genetics, University of Minnesota, 6 160 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
    Hum Mol Genet 20:R14-20. 2011
    ..Progress in delivery of SB transposons to liver for treatment of various systemic diseases, such as hemophilia and mucopolysaccharidoses types I and VII, has encountered some problems, but even here progress is being made...
  17. ncbi request reprint Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system
    Daniel F Carlson
    The Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Transgenic Res 20:1125-37. 2011
    ..Two strategies were employed to facilitate elimination of antibiotic genes from transgenic pigs, one based on Cre-recombinase and the other by segregation of independently transposed transgenes upon breeding...
  18. ncbi request reprint Phenotypic correction and long-term expression of factor VIII in hemophilic mice by immunotolerization and nonviral gene transfer using the Sleeping Beauty transposon system
    John R Ohlfest
    Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, 6 160 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
    Blood 105:2691-8. 2005
    ..Thus, SB provides a nonviral means for sustained FVIII gene delivery in a mouse model of hemophilia A if the immune response is prevented...
  19. ncbi request reprint Target-site preferences of Sleeping Beauty transposons
    Geyi Liu
    Department of Genetics, Cell Biology, and Development and The Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 346:161-73. 2005
    ..Our observations suggest that particular deformations of the double helix predicted by the V(step) algorithm can distinguish TA sites that vary by about 16-fold in their preferences for accommodating insertions of SB transposons...
  20. pmc Characterization of the human artemis promoter by heterologous gene expression in vitro and in vivo
    Megan M Multhaup
    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    DNA Cell Biol 30:751-61. 2011
    ..The human Artemis promoter thus provides sustained and moderate levels of gene expression that will be of significant utility for therapeutic gene transfer into hematopoeitic stem cells...
  21. pmc A transposon and transposase system for human application
    Perry B Hackett
    Department of Genetics, Cell Biology, and Development, Center for Genome Engineering, Institute of Human Genetics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 18:674-83. 2010
    ..This review examines the rationale and safety implications of application of the SB system to genetically modify T cells to be manufactured in compliance with current good manufacturing practice (cGMP) for phase I/II trials...
  22. ncbi request reprint Fishing for answers with transposons
    Shannon A Wadman
    Discovery Genomics, Inc, Minneapolis, MN 55413, USA
    Mar Biotechnol (NY) 7:135-41. 2005
    ..Results of these studies show that SB-mediated transgenesis is more efficient than that by injection of simple plasmids and that expression of transgenesis is stable and reliable following passage through the germline...
  23. ncbi request reprint Transposon vectors for gene-trap insertional mutagenesis in vertebrates
    Karl J Clark
    Department of Genetics, Cell Biology, and Development, Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Minneapolis, Minnesota, USA
    Genesis 39:225-33. 2004
    ....
  24. pmc Structure-based prediction of insertion-site preferences of transposons into chromosomes
    Aron M Geurts
    Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    Nucleic Acids Res 34:2803-11. 2006
    ..ProTIS analysis can be useful for functional genomic, gene transfer and human gene therapy applications using the SB system...
  25. pmc Excision of Sleeping Beauty transposons: parameters and applications to gene therapy
    Geyi Liu
    Department of Genetics, Cell Biology and Development and The Institute of Human Genetics, University of Minnesota, Minneapolis, MN 55455, USA
    J Gene Med 6:574-83. 2004
    ..The excision assay appears to be a relatively quick and easy method to optimize protocols for delivery of genes in SB transposons to mammalian chromosomes in living animals...
  26. ncbi request reprint Awakening gene therapy with Sleeping Beauty transposons
    Jeffrey J Essner
    Discovery Genomics Inc, Minneapolis, Minnesota 55413, USA
    Curr Opin Pharmacol 5:513-9. 2005
    ..Recent studies characterizing site selection for integration and insertional mutagenesis indicate that the Sleeping Beauty transposon system may be a safer alternative than viral approaches for gene therapy...
  27. ncbi request reprint Efficient gene delivery and gene expression in zebrafish using the Sleeping Beauty transposon
    Ann E Davidson
    The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, MN 55455, USA
    Dev Biol 263:191-202. 2003
    ....
  28. pmc Evaluating risks of insertional mutagenesis by DNA transposons in gene therapy
    Perry B Hackett
    Department of Genetics Cell Biology and Development, Center for Genome Engineering and Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
    Transl Res 161:265-83. 2013
    ..These findings are put into the context of the use of SB transposons in the treatment of human disease...
  29. pmc Precision editing of large animal genomes
    Wenfang Spring Tan
    Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
    Adv Genet 80:37-97. 2012
    ..Precision genetics also will enhance the development of therapeutic biomaterials and models of human disease as resources for the development of advanced patient therapies...
  30. pmc Transgene expression and silencing in a tick cell line: A model system for functional tick genomics
    Timothy J Kurtti
    Department of Entomology, University of Minnesota, Saint Paul, MN 55108, USA
    Insect Biochem Mol Biol 38:963-8. 2008
    ..SB transposition in cell line ISE6 provides an effective means to explore the functional genomics of I. scapularis...
  31. pmc Quantitative analysis of α-L-iduronidase expression in immunocompetent mice treated with the Sleeping Beauty transposon system
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development and the Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, United States of America
    PLoS ONE 8:e78161. 2013
    ..Cumulatively, our results allow transition to pre-clinical studies of SB-mediated alpha-L-iduronidase expression and correction of mucopolysaccharidosis type I in animal models. ..
  32. ncbi request reprint Expression of VE-cadherin in zebrafish embryos: a new tool to evaluate vascular development
    Jon D Larson
    Discovery Genomics, Incorporated, Minneapolis, Minnesota, USA
    Dev Dyn 231:204-13. 2004
    ..This loss can be restored in embryos supplemented with either zebrafish or human VEGF-A, the latter indicating that genes crucial to angiogenesis have highly conserved functional activities in vertebrates...
  33. ncbi request reprint Gene insertion and long-term expression in lung mediated by the Sleeping Beauty transposon system
    Lalitha R Belur
    Gene Therapy Program, Institute of Human Genetics, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:501-7. 2003
    ..We used a linker-mediated PCR technique to recover transposon flanking sequences, demonstrating transposition of pT/L into mouse chromosomal DNA of the lung...
  34. ncbi request reprint RecA-mediated, targeted mutagenesis in zebrafish
    Zongbin Cui
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, St Paul, MN 55108 1095, USA
    Mar Biotechnol (NY) 5:174-84. 2003
    ..Prior irradiation of the embryos with UV light enhanced RecA-mediated restoration of gene activity, suggesting that the effects we observed were augmented by one or more factors of zebrafish DNA repair systems...
  35. ncbi request reprint Gene transfer into genomes of human cells by the sleeping beauty transposon system
    Aron M Geurts
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:108-17. 2003
    ..With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy...
  36. pmc Mammalian germ-line transgenesis by transposition
    Adam J Dupuy
    The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 99:4495-9. 2002
    ..We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse...
  37. pmc Predicting preferential DNA vector insertion sites: implications for functional genomics and gene therapy
    Christopher S Hackett
    Biomedical Sciences Graduate Program and Department of Neurology, University of California San Francisco, Room U441K, Parnassus Ave, San Francisco, California 94143 0663, USA
    Genome Biol 8:S12. 2007
    ....
  38. ncbi request reprint Structure-function analysis of the inverted terminal repeats of the sleeping beauty transposon
    Zongbin Cui
    Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People s Republic of China
    J Mol Biol 318:1221-35. 2002
    ..These findings have led to the construction of a more advanced transposon that should be useful in gene transfer and insertional mutagenesis in vertebrates...

Research Grants1

  1. SB Transposons for Gene Therapy
    Perry B Hackett; Fiscal Year: 2010
    ..The goal of this grant is to determine whether transposons containing therapeutic genes can cure MPS disease in dogs as a model for human gene therapy. ..