Perry B HackettSummaryAffiliation: University of Minnesota Country: USA Publications
Research Grants
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Publications
Integrating DNA vectors for gene therapyPerry B Hackett
Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota, USA
Mol Ther 15:10-2. 2007- The Sleeping Beauty transposon system: a non-viral vector for gene therapyElena L Aronovich
Department of Genetics, Cell Biology and Development, The Center for Genome Engineering, Institute of Human Genetics, University of Minnesota, 6 160 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
Hum Mol Genet 20:R14-20. 2011..Progress in delivery of SB transposons to liver for treatment of various systemic diseases, such as hemophilia and mucopolysaccharidoses types I and VII, has encountered some problems, but even here progress is being made... - Systemic correction of storage disease in MPS I NOD/SCID mice using the sleeping beauty transposon systemElena L Aronovich
Department of Genetics, Cell Biology and Development, Center for Genome Engineering, University of Minnesota, Minneapolis, 55455, USA
Mol Ther 17:1136-44. 2009.... - Transgene expression and silencing in a tick cell line: A model system for functional tick genomicsTimothy J Kurtti
Department of Entomology, University of Minnesota, Saint Paul, MN 55108, USA
Insect Biochem Mol Biol 38:963-8. 2008..SB transposition in cell line ISE6 provides an effective means to explore the functional genomics of I. scapularis... - Excision of Sleeping Beauty transposons: parameters and applications to gene therapyGeyi Liu
Department of Genetics, Cell Biology and Development and The Institute of Human Genetics, University of Minnesota, Minneapolis, MN 55455, USA
J Gene Med 6:574-83. 2004..The excision assay appears to be a relatively quick and easy method to optimize protocols for delivery of genes in SB transposons to mammalian chromosomes in living animals... 
Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expressionPaul R Score
Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
Mol Ther 13:617-24. 2006..The results also provide additional evidence for the effectiveness of the Sleeping Beauty transposon system as an in vivo DNA-mediated gene transfer strategy for achieving long-term expression...- Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injectionJason B Bell
Department of Genetics, Cell Biology and Development, Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, 6 160 Jackson Hall, 321 Church Street SE, Minneapolis, Minnesota 55455, USA
Nat Protoc 2:3153-65. 2007..Several weeks after injection, transgene expression stabilizes at approximately 1% of the level at 24 h, presumably owing to integration of the transposons into chromosomes... - A transposon and transposase system for human applicationPerry B Hackett
Department of Genetics, Cell Biology, and Development, Center for Genome Engineering, Institute of Human Genetics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
Mol Ther 18:674-83. 2010..This review examines the rationale and safety implications of application of the SB system to genetically modify T cells to be manufactured in compliance with current good manufacturing practice (cGMP) for phase I/II trials... - Gene expression in lung and liver after intravenous infusion of polyethylenimine complexes of Sleeping Beauty transposonsKelly M Podetz-Pedersen
Beckman Center for Transposon Research, Center for Genome Engineering, Institute of Human Genetics, and Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
Hum Gene Ther 21:210-20. 2010..These results provide insight into the relative effectiveness and organ specificity of these two methods of nonviral gene delivery when coupled with the Sleeping Beauty transposon system... - Sleeping beauty transposon-mediated gene therapy for prolonged expressionPerry B Hackett
Department of Genetics, Cell Biology and Development Arnold and Mabel Beckman Center for Transposon Research University of Minnesota Minneapolis, Minnesota 55455, USA
Adv Genet 54:189-232. 2005..Here we review the activities of the SB system in mice used as a model for human gene therapy, methods of delivery of the SB system, and its efficacy in ameliorating disorders that model human disease... - Awakening gene therapy with Sleeping Beauty transposonsJeffrey J Essner
Discovery Genomics Inc, Minneapolis, Minnesota 55413, USA
Curr Opin Pharmacol 5:513-9. 2005..Recent studies characterizing site selection for integration and insertional mutagenesis indicate that the Sleeping Beauty transposon system may be a safer alternative than viral approaches for gene therapy... - Fishing for answers with transposonsShannon A Wadman
Discovery Genomics, Inc, Minneapolis, MN 55413, USA
Mar Biotechnol (NY) 7:135-41. 2005..Results of these studies show that SB-mediated transgenesis is more efficient than that by injection of simple plasmids and that expression of transgenesis is stable and reliable following passage through the germline... - Structure-based prediction of insertion-site preferences of transposons into chromosomesAron M Geurts
Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
Nucleic Acids Res 34:2803-11. 2006..ProTIS analysis can be useful for functional genomic, gene transfer and human gene therapy applications using the SB system... - RecA-mediated, targeted mutagenesis in zebrafishZongbin Cui
Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, St. Paul, MN 55108-1095, USA
Mar Biotechnol (NY) 5:174-84. 2003..Prior irradiation of the embryos with UV light enhanced RecA-mediated restoration of gene activity, suggesting that the effects we observed were augmented by one or more factors of zebrafish DNA repair systems... - Prolonged expression of a lysosomal enzyme in mouse liver after Sleeping Beauty transposon-mediated gene delivery: implications for non-viral gene therapy of mucopolysaccharidosesElena L Aronovich
Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
J Gene Med 9:403-15. 2007..The Sleeping Beauty (SB) transposon system is a non-viral vector system that can integrate precise sequences into chromosomes. We evaluated the SB transposon system as a tool for gene therapy of mucopolysaccharidosis (MPS) types I and VII... - Gene transfer into genomes of human cells by the sleeping beauty transposon systemAron M Geurts
Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
Mol Ther 8:108-17. 2003..With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy... - RNA as a source of transposase for Sleeping Beauty-mediated gene insertion and expression in somatic cells and tissuesAndrew Wilber
The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
Mol Ther 13:625-30. 2006..We conclude that in vitro-transcribed mRNA can be used as an effective source of transposase for SB-mediated transposition in mammalian cells and tissues... - Gene insertion and long-term expression in lung mediated by the Sleeping Beauty transposon systemLalitha R Belur
Gene Therapy Program, Institute of Human Genetics, Minneapolis, Minnesota 55455, USA
Mol Ther 8:501-7. 2003..We used a linker-mediated PCR technique to recover transposon flanking sequences, demonstrating transposition of pT/L into mouse chromosomal DNA of the lung... - Counterselection and co-delivery of transposon and transposase functions for Sleeping Beauty-mediated transposition in cultured mammalian cellsAndrea D Converse
Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, 6-160 Jackson Hall 321 Church Street S.E, Minneapolis, MN, 55455, USA
Biosci Rep 24:577-94. 2004.... - Target-site preferences of Sleeping Beauty transposonsGeyi Liu
Department of Genetics, Cell Biology, and Development and The Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Minneapolis, MN 55455, USA
J Mol Biol 346:161-73. 2005..Our observations suggest that particular deformations of the double helix predicted by the V(step) algorithm can distinguish TA sites that vary by about 16-fold in their preferences for accommodating insertions of SB transposons... - Phenotypic correction and long-term expression of factor VIII in hemophilic mice by immunotolerization and nonviral gene transfer using the Sleeping Beauty transposon systemJohn R Ohlfest
Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, 6 160 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
Blood 105:2691-8. 2005..Thus, SB provides a nonviral means for sustained FVIII gene delivery in a mouse model of hemophilia A if the immune response is prevented... - Transposon vectors for gene-trap insertional mutagenesis in vertebratesKarl J Clark
Department of Genetics, Cell Biology, and Development, Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Minneapolis, Minnesota, USA
Genesis 39:225-33. 2004.... - Efficient gene delivery and gene expression in zebrafish using the Sleeping Beauty transposonAnn E Davidson
The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, MN 55455, USA
Dev Biol 263:191-202. 2003.... - Harnessing a high cargo-capacity transposon for genetic applications in vertebratesDarius Balciunas
The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America
PLoS Genet 2:e169. 2006..Together, Tol2 provides a novel nonviral vector for the delivery of large genetic payloads for gene therapy and other transgenic applications... - Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposonAron M Geurts
Department of Genetics, Cell Biology, and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
BMC Biotechnol 6:30. 2006..We sought to generate a transposon system that would be capable of reporting the expression pattern of a mouse gene while allowing for conditional expression of a gene of interest in a tissue- or temporal-specific pattern... - Mammalian germ-line transgenesis by transpositionAdam J Dupuy
The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
Proc Natl Acad Sci U S A 99:4495-9. 2002..We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse... - Predicting preferential DNA vector insertion sites: implications for functional genomics and gene therapyChristopher S Hackett
Biomedical Sciences Graduate Program and Department of Neurology, University of California San Francisco, Room U441K, Parnassus Ave, San Francisco, California 94143 0663, USA
Genome Biol 8:S12. 2007.... - Structure-function analysis of the inverted terminal repeats of the sleeping beauty transposonZongbin Cui
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China
J Mol Biol 318:1221-35. 2002..These findings have led to the construction of a more advanced transposon that should be useful in gene transfer and insertional mutagenesis in vertebrates...
Research Grants
- SB Transposons for Gene TherapyPerry B Hackett; Fiscal Year: 2010..The goal of this grant is to determine whether transposons containing therapeutic genes can cure MPS disease in dogs as a model for human gene therapy. ..