Affiliation: University of California
- Essential yeast protein with unexpected similarity to subunits of mammalian cleavage and polyadenylation specificity factor (CPSF)G Chanfreau
Department of Biochemistry and Biophysics, UCSF School of Medicine, San Francisco, CA 94143 0448
Science 274:1511-4. 1996..These results demonstrate a structural and functional conservation of the yeast and mammalian 3'-end processing machineries despite a lack of conservation of the cis sequences...
- Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for sub2, an essential spliceosomal ATPaseA L Kistler
Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94143 0448, USA
Genes Dev 15:42-9. 2001..We propose that Sub2 functionally interacts with Mud2 both before and after PS formation. In the absence of Mud2, Sub2 function becomes dispensable...
- Conservation in budding yeast of a kinase specific for SR splicing factorsC W Siebel
Department of Biochemistry and Biophysics, University of California, 513 Parnassus Avenue, San Francisco, CA 94143 0448, USA
Proc Natl Acad Sci U S A 96:5440-5. 1999..The unexpected discovery of an SR protein kinase in budding yeast provides a foundation for genetic dissection of the biological functions of SR proteins and their kinases...
- An essential nuclear envelope integral membrane protein, Brr6p, required for nuclear transportA de Bruyn Kops
Department of Biochemistry and Biophysics, UCSF Medical School, 513 Parnassus Avenue, San Francisco, CA 94143, USA
EMBO J 20:4183-93. 2001..We hypothesize that Brr6p is located adjacent to the nuclear pore and interacts functionally with the pore and transport machinery...
- A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particlesP L Raghunathan
University of California, San Francisco, School of Medicine, Department of Biochemistry and Biophysics, San Francisco, CA 94143 0448, USA
Science 279:857-60. 1998..Addition of purified Prp24 protein regenerates duplex U4/U6 snRNPs for new rounds of splicing. The reannealing reaction catalyzed by Prp24 proceeds more efficiently with snRNPs than with deproteinized snRNAs...
- Mechanical devices of the spliceosome: motors, clocks, springs, and thingsJ P Staley
Department of Biochemistry and Biophysics, University of California, San Francisco, 94143, USA
Cell 92:315-26. 1998
- Alternative 3'-end processing of U5 snRNA by RNase IIIG Chanfreau
Department of Biochemistry and Biophysics, University of California School of Medicine, San Francisco, California 94143 0448 USA
Genes Dev 11:2741-51. 1997..These results identify RNase III as a trans-acting factor involved in 3'-end formation of snRNA and show how RNase III might regulate alternative RNA processing pathways...