Research Topics
Genomes and GenesSpecies | Margaret L GulleySummaryAffiliation: University of North Carolina Country: USA Publications
| Collaborators
|
Detail Information
Publications
Epstein-barr virus infected gastric adenocarcinoma expresses latent and lytic viral transcripts and has a distinct human gene expression profileWeihua Tang
Department of Pathology and Laboratory Medicine, Lineberger Comprehensive Cancer Center, University of North Carolina, 913 Brinkhous Bullitt Building, Chapel Hill, NC, 27599 7525, USA
Infect Agent Cancer 7:21. 2012..EBV DNA is found within the malignant cells of 10% of gastric cancers. Modern molecular technology facilitates identification of virus-related biochemical effects that could assist in early diagnosis and disease management...- Genetic tests to evaluate prognosis and predict therapeutic response in acute myeloid leukemiaMargaret L Gulley
Department of Pathology and Laboratory Medicine, 913 Brinkhous Bullitt Building, University of North Carolina, Chapel Hill, NC 27599 7525, USA
J Mol Diagn 12:3-16. 2010..Acute myeloid leukemia leads the way for modern molecular medicine... 
Tumor origin and CD20 expression in posttransplant lymphoproliferative disorder occurring in solid organ transplant recipients: implications for immune-based therapyMargaret L Gulley
Department of Pathology, University of North Carolina at Chapel Hill and the Lineberger Comprehensive Cancer Center, Chapel Hill, NC 27599 7525, USA
Transplantation 76:959-64. 2003..The efficacy of anti-CD20 therapy, on the other hand, depends on CD20 expression by neoplastic cells. Only limited prior data exist regarding either of these parameters...
A rational approach to genetic testing for sarcomaMargaret L Gulley
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599 7525, USA
Diagn Mol Pathol 18:1-10. 2009....- Using Epstein-Barr viral load assays to diagnose, monitor, and prevent posttransplant lymphoproliferative disorderMargaret L Gulley
Department of Pathology and Laboratory Medicine, University of North Carolina, 101 Manning Dr, 913 Brinkhous Bullitt Building, Chapel Hill, NC 27599 7525, USA
Clin Microbiol Rev 23:350-66. 2010..Restoring T cell immunity against EBV is a major strategy for overcoming PTLD, and novel EBV-directed therapies are being explored to thwart virus-driven neoplasia... - Validation of Roche LightCycler Epstein-Barr virus quantification reagents in a clinical laboratory settingMargaret L Gulley
Dept of Pathology, University of North Carolina, 101 Manning Dr, 913 Brinkhous Bullitt Building, Chapel Hill, NC 27599 7525, USA
J Mol Diagn 8:589-97. 2006..In conclusion, this LightCycler EBV assay is rapid, sensitive, and linear for quantifying EBV viral load. The assay appears to be useful for measuring clinically significant EBV levels in immunodeficient patients... - Clinical laboratory reports in molecular pathologyMargaret L Gulley
Department of Pathology, 913 Brinkhous Bullitt Bldg, University of North Carolina, Chapel Hill, NC 27599 7525, USA
Arch Pathol Lab Med 131:852-63. 2007..The recent introduction of array technology has added another layer of complexity involving massive parallel analysis of multiple genes, transcripts, or proteins... - Epstein-Barr virus-specific methylation of human genes in gastric cancer cellsJulie L Ryan
Department of Pathology and Laboratory Medicine and The Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7525, USA
Infect Agent Cancer 5:27. 2010..abstract:.. - Laboratory assays for Epstein-Barr virus-related diseaseMargaret L Gulley
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599 7525, USA
J Mol Diagn 10:279-92. 2008.... - Public policy recommendations for oversight of molecular laboratory testsMargaret L Gulley
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, NC 27599-7525, USA
N C Med J 68:109-11. 2007 - Epstein-Barr virus quantitation by real-time PCR targeting multiple gene segments: a novel approach to screen for the virus in paraffin-embedded tissue and plasmaJulie L Ryan
Department of Pathology, University of North Carolina at Chapel Hill, NC 27599-7525, USA
J Mol Diagn 6:378-85. 2004..The findings suggest that Q-PCR is an effective method of distinguishing disease-associated virus from incidental virus in paraffin-embedded tissue and in plasma samples... - Epstein-Barr viral load as a marker of lymphoma in AIDS patientsHongxin Fan
University of North Carolina at Chapel Hill and the Lineberger Comprehensive Cancer Center, Chapel Hill, NC 27599, USA
J Med Virol 75:59-69. 2005..There was no association between EBV viral load and human immunodeficiency virus (HIV) load or CD4 count. In conclusion, EBV viral load shows promise as a tool to assist in diagnosis and management of EBV-related lymphoma patients... - Epstein-Barr Virus (EBV) DNA in plasma is not encapsidated in patients with EBV-related malignanciesJulie L Ryan
Department of Pathology, University of North Carolina at Chapel Hill, 27599, USA
Diagn Mol Pathol 13:61-8. 2004..These findings may be important in understanding how viral load relates to disease status and in predicting response to nucleoside analogs and other antiviral therapies... - Laboratory markers of tumor burden in nasopharyngeal carcinoma: a comparison of viral load and serologic tests for Epstein-Barr virusHongxin Fan
Department of Pathology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Int J Cancer 112:1036-41. 2004..EBV viral load was more informative than was EA serology for distinguishing remission from relapsed disease. EBV DNA measurement appears to be a noninvasive way to monitor tumor burden after therapy... - Epstein-Barr virus lytic infection contributes to lymphoproliferative disease in a SCID mouse modelGregory K Hong
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, Chapel Hill, NC 27599, USA
J Virol 79:13993-4003. 2005..These results suggest that lytic EBV gene expression contributes to EBV-associated lymphoproliferative disease, potentially through induction of paracrine B-cell growth factors... - Analytic validation of a quantitative real-time PCR assay to measure CMV viral load in whole bloodLeigh B Thorne
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599 7525, USA
Diagn Mol Pathol 16:73-80. 2007..In summary, CMV viral load measurement targeting the polymerase gene is nearly equivalent to the antigenemia assay for detecting and monitoring active CMV infection in whole blood samples... - Real-time PCR measures Epstein-Barr Virus DNA in archival breast adenocarcinomasLeigh B Thorne
Department of Pathology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina 27599-7525, USA
Diagn Mol Pathol 14:29-33. 2005..The low levels probably confounded our ability to localize the virus to particular cell types or to characterize viral gene expression... - Cytomegalovirus DNA measurement in blood and plasma using Roche LightCycler CMV quantification reagentsWeihua Tang
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Diagn Mol Pathol 17:166-73. 2008..The LightCycler CMV assay is rapid, sensitive, and linear for quantifying CMV viral load, and it seems to be useful in the diagnosis and monitoring of affected patients... - Detection of clonal IGH gene rearrangements: summary of molecular oncology surveys of the College of American PathologistsMarina N Nikiforova
Department of Pathology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
Arch Pathol Lab Med 131:185-9. 2007.... - Post-transplantation lymphoproliferative disease: Epstein-Barr virus DNA levels, HLA-A3, and survivalStephen A Wheless
The School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Am J Respir Crit Care Med 178:1060-5. 2008..Elevation in Epstein-Barr virus (EBV) circulating DNA has been proposed as a marker for development of post-transplant lymphoproliferative disease (PTLD), but few published data exist in the study of lung-transplant recipients... - Differential signaling pathways are activated in the Epstein-Barr virus-associated malignancies nasopharyngeal carcinoma and Hodgkin lymphomaJennifer A Morrison
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA
Cancer Res 64:5251-60. 2004..These findings point to a divergence in pathways activated by EBV in different cellular contexts... - Viral response to chemotherapy in endemic burkitt lymphomaWeihua Tang
Departments of Pathology and Laboratory Medicine, Otolaryngology Head and Neck Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7525, USA
Clin Cancer Res 16:2055-64. 2010..We studied whether cyclophosphamide chemotherapy induces EBV to switch from latent to lytic phases of infection in a series of EBV-associated Burkitt lymphomas... - Quality assurance of RNA expression profiling in clinical laboratoriesWeihua Tang
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599 7525, USA
J Mol Diagn 14:1-11. 2012..Careful attention to the well-honed principles of laboratory medicine, along with guidance from government and professional groups on strategies to preserve RNA and manage large data sets, promotes clinical-grade assay performance... - Multiple pathways for Epstein-Barr virus episome loss from nasopharyngeal carcinomaDirk P Dittmer
Lineberger Comprehensive Cancer Center, Center for AIDS Research and Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC, USA
Int J Cancer 123:2105-12. 2008..The conceptual insights provided by our quantitative analysis should aid our understanding of mammalian episomes, as well as lead to designs to cure latent viral infection... - Epstein-Barr virus latent membrane protein 1 is not associated with vessel density nor with hypoxia inducible factor 1 alpha expression in nasopharyngeal carcinoma tissueAlexander A Benders
Department of Pathology and Laboratory Medicine, The Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, 27599 7525, USA
Head Neck Pathol 3:276-82. 2009..There is no consistent relationship between LMP1 and either HIF-1alpha expression or degree of microvasculature. The biologic basis for the wide variation in vessel density deserves further investigation... - Clinical applications of BCR-ABL molecular testing in acute leukemiaAmgad L Nashed
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7525, USA
J Mol Diagn 5:63-72. 2003..On the horizon are gene expression profiles that will likely provide additional information beyond what is obtainable with current clinical and laboratory approaches... - UGT1A1 promoter genotype is not strongly associated with severity of coronary artery diseaseMichael J Papez
Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Diagn Mol Pathol 18:226-31. 2009..These findings suggest that UGT1A1 promoter genotype is not a major factor contributing to risk of CAD... - Recommended principles and practices for validating clinical molecular pathology testsLawrence Jennings
Department of Pathology and Laboratory Medicine, Children s Memorial Hospital, Chicago, Illinois, USA
Arch Pathol Lab Med 133:743-55. 2009..Analytic and clinical performance characteristics as well as test limitations are established and documented through the process of test validation... - No evidence of Epstein-Barr virus association with Merkel cell carcinomaMaren Shaw
Department of Dermatology, University of North Carolina, Chapel Hill School of Medicine, Chapel Hill, NC 27599-7525, USA
J Cutan Pathol 33:624-8. 2006..RESULTS: Positive controls reacted appropriately. However, there was no evidence of latent or lytic EBV in any of the MCC biopsies or other cutaneous tumors. CONCLUSION: Our findings suggest that EBV is not associated with MCC... - Use of adenovirus vectors expressing Epstein-Barr virus (EBV) immediate-early protein BZLF1 or BRLF1 to treat EBV-positive tumorsWen-hai Feng
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599-7295, USA
J Virol 76:10951-9. 2002..The addition of ganciclovir did not significantly affect the antitumor effect of the BZLF1 and BRLF1 adenovirus vectors. These results suggest a potential cancer therapy against EBV-related tumors... - Systematic bias in genomic classification due to contaminating non-neoplastic tissue in breast tumor samplesFathi Elloumi
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
BMC Med Genomics 4:54. 2011..This "normal" tissue could represent a source of non-random error or systematic bias in genomic classification... - Diagnosis of human congenital cytomegalovirus infection by amplification of viral DNA from dried blood spots on perinatal cardsLori Scanga
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, 27599-7525, USA
J Mol Diagn 8:240-5. 2006..Further studies are warranted on patients with hearing loss or other neurological deficits to determine the percentage that is attributable to congenital HCMV infection... - Epstein-Barr virus lytic infection is required for efficient production of the angiogenesis factor vascular endothelial growth factor in lymphoblastoid cell linesGregory K Hong
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, Chapel Hill, NC 27599, USA
J Virol 79:13984-92. 2005..In addition, as VEGF is a pleiotropic factor with effects other than angiogenesis, lytically induced VEGF secretion may potentially contribute to viral pathogenesis... - T-cell blast crisis of chronic myelogenous leukemia manifesting as a large mediastinal tumorCharles C Ye
Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
Hum Pathol 33:770-3. 2002.... - BRAF mutation testing in colorectal cancerShree G Sharma
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, USA
Arch Pathol Lab Med 134:1225-8. 2010.... - Standard mutation nomenclature in molecular diagnostics: practical and educational challengesShuji Ogino
Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, 75 Francis St, Boston, MA 02115, USA
J Mol Diagn 9:1-6. 2007..Our aim is to alert laboratory scientists and other health care professionals to the important issues and provide a foundation for further discussions that will ultimately lead to solutions... - Detection of common disease-causing mutations in mitochondrial DNA (mitochondrial encephalomyopathy, lactic acidosis with stroke-like episodes MTTL1 3243 A>G and myoclonic epilepsy associated with ragged-red fibers MTTK 8344A>G) by real-time polymerase chHongxin Fan
Department of Pathology and Laboratory Medicine, The University of North Carolina, Chapel Hill, 27599 7525, USA
J Mol Diagn 8:277-81. 2006..This LightCycler assay is a rapid and reliable technique for molecular diagnosis of these mitochondrial gene mutations... - Guidelines for interpreting EBER in situ hybridization and LMP1 immunohistochemical tests for detecting Epstein-Barr virus in Hodgkin lymphomaMargaret L Gulley
Department of Pathology, University of Texas Health Science Center at San Antonio, USA
Am J Clin Pathol 117:259-67. 2002..EBER and LMP1 assays in combination are more effective than either assay alone for identifying EBV-related Hodgkin lymphoma... - The usefulness of cytogenetic analysis in fine needle aspirates for the histologic subtyping of sarcomasScott E Kilpatrick
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC, USA
Mod Pathol 19:815-9. 2006..However, when cell block material is available for immunohistochemistry, the majority of such cases are successfully subtyped... - Distribution of Epstein-Barr viral load in serum of individuals from nasopharyngeal carcinoma high-risk families in TaiwanXiaohong Yang
Genetic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20852, USA
Int J Cancer 118:780-4. 2006.... - Epstein-Barr virus as a marker of survival after Hodgkin's lymphoma: a population-based studyTheresa H M Keegan
Northern California Cancer Center, 2201 Walnut Ave, Ste 300, Fremont, CA 94538 2334, USA
J Clin Oncol 23:7604-13. 2005..This study assessed survival after HL in a population-based cohort large enough to examine the joint effects of EBV with other factors including age, sex, and histologic subtype... - Quantitation of CMV by real-time PCR in transfusable RBC unitsDeborah J Greenlee
University of Texas Health Science Center at San Antonio, USA
Transfusion 42:403-8. 2002..Future clinical studies on larger numbers of units are required to determine the utility of real-time PCR in evaluating the risk of CMV transmission and in confirming the efficacy of WBC reduction... - Clonal evolution of lymphoblastoid cell linesJulie L Ryan
Department of Dermatology, University of Rochester James P. Wilmot Cancer Center, Rochester, NY, USA
Lab Invest 86:1193-200. 2006.... - Detection of bcl-2/J(H) translocation by polymerase chain reaction: a summary of the experience of the Molecular Oncology Survey of the College of American PathologistEric D Hsi
Department of Clinical Pathology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
Arch Pathol Lab Med 126:902-8. 2002.... - Racial/ethnic variation in EBV-positive classical Hodgkin lymphoma in California populationsSally L Glaser
Northern California Cancer Center, Fremont, CA 94538, USA
Int J Cancer 123:1499-507. 2008.... - False-negative factor V Leiden genetic testing in a patient with recurrent deep venous thrombosisEdward N Libby
Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131 0001, USA
Am J Hematol 81:284-9. 2006..There is a need for standardization of optimized fVL genetic testing methods. Further education of ordering physicians on the limitations of genetic testing is necessary... - Population-based patterns of human immunodeficiency virus-related Hodgkin lymphoma in the Greater San Francisco Bay Area, 1988-1998Sally L Glaser
Northern California Cancer Center, Union City, California 94587, USA
Cancer 98:300-9. 2003.... - Epstein-Barr virus and breast cancer: state of the evidence for viral carcinogenesisSally L Glaser
Northern California Cancer Center, 32960 Alvarado Niles Road, Ste 600, P O Box 5033, Union City, CA 94587, USA
Cancer Epidemiol Biomarkers Prev 13:688-97. 2004..Further research is warranted, given the potential for an EBV association to inform not only breast cancer etiology but also early detection, treatment, and prevention... - Inter- and intra-observer reliability of Epstein-Barr virus detection in Hodgkin lymphoma using histochemical proceduresSally L Glaser
Northern California Cancer Center, 32960 Alvarado-Niles Road, Suite 600, Union City, CA 94587, USA
Leuk Lymphoma 45:489-97. 2004..The variability in assay interpretation justifies caution in comparing EBV association results across HL studies and underscores the need for interpretation guidelines... - Lymphoid tissues from patients with infectious mononucleosis lack monoclonal B and T cellsJulie A Plumbley
University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
J Mol Diagn 4:37-43. 2002..In this case series, no monoclonal lymphoid populations were identified in any case of IM. This finding suggests that molecular studies are useful in distinguishing IM from lymphoid neoplasms...