Lan Guan

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Lessons from lactose permease
    Lan Guan
    Department of Physiology, University of California, Los Angeles, California 90095 1662, USA
    Annu Rev Biophys Biomol Struct 35:67-91. 2006
  2. pmc Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition
    Vincent Chaptal
    Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 108:9361-6. 2011
  3. ncbi request reprint Sugar recognition by the lactose permease of Escherichia coli
    José Luis Vázquez-Ibar
    Department of Physiology and Microbiology, Molecular Biology Institute, Howard Hughes Medical Institute, Los Angeles, CA 90095 1662, USA
    J Biol Chem 279:49214-21. 2004
  4. pmc An early event in the transport mechanism of LacY protein: interaction between helices V and I
    Yonggang Zhou
    Department of Physiology, University of California, Los Angeles, California 90095 1662, USA
    J Biol Chem 286:30415-22. 2011
  5. pmc Evidence for an intermediate conformational state of LacY
    Xiaoxu Jiang
    Department of Physiology, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 109:E698-704. 2012
  6. pmc Exploiting luminescence spectroscopy to elucidate the interaction between sugar and a tryptophan residue in the lactose permease of Escherichia coli
    José Luis Vázquez-Ibar
    Howard Hughes Medical Institute, and Department of Physiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 100:12706-11. 2003
  7. ncbi request reprint Aromatic stacking in the sugar binding site of the lactose permease
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 42:1377-82. 2003
  8. pmc Binding affinity of lactose permease is not altered by the H+ electrochemical gradient
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 101:12148-52. 2004
  9. pmc Properties of a LacY efflux mutant
    Lan Guan
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 48:9250-5. 2009
  10. pmc Structural determination of wild-type lactose permease
    Lan Guan
    Department of Physiology, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 104:15294-8. 2007

Collaborators

Detail Information

Publications21

  1. pmc Lessons from lactose permease
    Lan Guan
    Department of Physiology, University of California, Los Angeles, California 90095 1662, USA
    Annu Rev Biophys Biomol Struct 35:67-91. 2006
    ..A working model for the mechanism is presented that involves alternating access of both the sugar- and H+-binding sites to either side of the membrane...
  2. pmc Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition
    Vincent Chaptal
    Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 108:9361-6. 2011
    ..E269, a residue essential for binding, coordinates the C-4 hydroxyl of the galactopyranoside moiety. The location of the sugar is in accord with many biochemical studies...
  3. ncbi request reprint Sugar recognition by the lactose permease of Escherichia coli
    José Luis Vázquez-Ibar
    Department of Physiology and Microbiology, Molecular Biology Institute, Howard Hughes Medical Institute, Los Angeles, CA 90095 1662, USA
    J Biol Chem 279:49214-21. 2004
    ....
  4. pmc An early event in the transport mechanism of LacY protein: interaction between helices V and I
    Yonggang Zhou
    Department of Physiology, University of California, Los Angeles, California 90095 1662, USA
    J Biol Chem 286:30415-22. 2011
    ..This ligand-induced movement may act to initiate the global conformational change resulting from sugar binding...
  5. pmc Evidence for an intermediate conformational state of LacY
    Xiaoxu Jiang
    Department of Physiology, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 109:E698-704. 2012
    ..Notably, branched-chain or neopentyl glycol maltoside detergents stabilize Cys154 → Gly LacY in the membrane-embedded form...
  6. pmc Exploiting luminescence spectroscopy to elucidate the interaction between sugar and a tryptophan residue in the lactose permease of Escherichia coli
    José Luis Vázquez-Ibar
    Howard Hughes Medical Institute, and Department of Physiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 100:12706-11. 2003
    ..The spectroscopic data fully complement the x-ray structure and demonstrate the feasibility of fluorescence spectroscopy for studying sugar-protein interactions...
  7. ncbi request reprint Aromatic stacking in the sugar binding site of the lactose permease
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 42:1377-82. 2003
    ..The results are discussed in the context of a current model for the binding site...
  8. pmc Binding affinity of lactose permease is not altered by the H+ electrochemical gradient
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 101:12148-52. 2004
    ....
  9. pmc Properties of a LacY efflux mutant
    Lan Guan
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 48:9250-5. 2009
    ..Taken as a whole, the results suggest that the primary defect induced by the mutation may involve a decrease in affinity for H(+)...
  10. pmc Structural determination of wild-type lactose permease
    Lan Guan
    Department of Physiology, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 104:15294-8. 2007
    ..Because the inward-facing conformation represents the lowest free-energy state, the rate-limiting step for transport may be the conformational change leading to the outward-facing conformation...
  11. pmc Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 99:3475-80. 2002
    ..The findings confirm previous observations indicating that intermolecular cross-linking is a stochastic process resulting from random collisions and support a number of other lines of evidence that lactose permease is a monomer...
  12. pmc Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins
    Lan Guan
    Department of Physiology, University of California Los Angeles, Los Angeles, California, USA
    Nat Protoc 2:2012-7. 2007
    ..Gels are dried and exposed to a PhosphoImager screen for 1-5 d, and incorporation of radioactivity is visualized. Initial results can be obtained in 24 h...
  13. pmc Intermolecular thiol cross-linking via loops in the lactose permease of Escherichia coli
    Natalia Ermolova
    Howard Hughes Medical Institute, Department of Physiology and Microbiology, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 100:10187-92. 2003
    ..In addition, evidence is presented indicating that reentrance loops are unlikely in this polytopic membrane transport protein...
  14. ncbi request reprint Probing the mechanism of a membrane transport protein with affinity inactivators
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, UCLA, Los Angeles, California 90095 1662, USA
    J Biol Chem 278:10641-8. 2003
    ..Thus, these inactivators behave as unique suicide substrates...
  15. pmc Opening and closing of the periplasmic gate in lactose permease
    Yonggang Zhou
    Departments of Physiology and Microbiology, Immunology, and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 105:3774-8. 2008
    ..The results provide direct support for the argument that transport via LacY involves opening and closing of a large periplasmic cavity...
  16. pmc Manipulating phospholipids for crystallization of a membrane transport protein
    Lan Guan
    Department of Physiology, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 103:1723-6. 2006
    ..Thus, it is likely that manipulation of PL is a good strategy for predominantly hydrophobic membrane proteins like LacY...
  17. pmc Changing the lactose permease of Escherichia coli into a galactose-specific symporter
    Lan Guan
    Howard Hughes Medical Institute, Departments of Physiology and Microbiology and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1662
    Proc Natl Acad Sci U S A 99:6613-8. 2002
    ..This is in contrast to Cys-148, a neighboring residue in helix V, that interacts with the hydrophobic face of the galactosyl moiety of D-galactopyranosides...
  18. ncbi request reprint Helices VII and X in the lactose permease of Escherichia coli: proximity and ligand-induced distance changes
    Wei Zhang
    Howard Hughes Medical Institute, Departments of Physiology and Microbiology and Molecular Genetics Molecular Biology Institute, Los Angeles, CA, 90095 1622, USA
    J Mol Biol 315:53-62. 2002
    ....
  19. pmc Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY
    Osman Mirza
    Department of Biological Sciences, Membrane Protein Crystallography Group, Imperial College London, London, UK
    EMBO J 25:1177-83. 2006
    ..Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction...
  20. pmc An approach to membrane protein structure without crystals
    Paul L Sorgen
    Biochemistry Department, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 99:14037-40. 2002
    ..Here we demonstrate that this information is sufficient to calculate a structural model at the level of helix packing or better...
  21. pmc Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli
    Orsolya Kiraly
    Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
    Protein Expr Purif 48:104-11. 2006
    ..The use of the novel pepP knock-out E. coli strain should be generally applicable to the expression of recombinant proteins, which undergo unwanted N-terminal trimming by aminopeptidase P...