B R Grubb

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Transgenic hCFTR expression fails to correct β-ENaC mouse lung disease
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Lung Cell Mol Physiol 302:L238-47. 2012
  2. ncbi request reprint In vivo microdialysis for determination of nasal liquid ion composition
    Barbara R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Am J Physiol Cell Physiol 282:C1423-31. 2002
  3. ncbi request reprint Culture of murine nasal epithelia: model for cystic fibrosis
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, 7011 Thurston Bowles Bldg, CB 7248, Univ of North Carolina, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Lung Cell Mol Physiol 290:L270-7. 2006
  4. pmc SERCA pump inhibitors do not correct biosynthetic arrest of deltaF508 CFTR in cystic fibrosis
    Barbara R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, 7011 Thurston Bowles Bldg, CB 7248, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    Am J Respir Cell Mol Biol 34:355-63. 2006
  5. ncbi request reprint Olfactory epithelia exhibit progressive functional and morphological defects in CF mice
    Barbara R Grubb
    CF Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Cell Physiol 293:C574-83. 2007
  6. ncbi request reprint Alterations in airway ion transport in NKCC1-deficient mice
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
    Am J Physiol Cell Physiol 281:C615-23. 2001
  7. ncbi request reprint Mucociliary transport determined by in vivo microdialysis in the airways of normal and CF mice
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, The Univ of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Lung Cell Mol Physiol 286:L588-95. 2004
  8. ncbi request reprint Intestinal ion transport in NKCC1-deficient mice
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
    Am J Physiol Gastrointest Liver Physiol 279:G707-18. 2000
  9. ncbi request reprint Ion transport across the normal and CF neonatal murine intestine
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    Am J Physiol 277:G167-74. 1999
  10. pmc Ion transport across CF and normal murine olfactory and ciliated epithelium
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, 7011 Thurston Bowles Bldg, CB 7248, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolia 27599 7248, USA
    Am J Physiol Cell Physiol 296:C1301-9. 2009

Detail Information

Publications33

  1. pmc Transgenic hCFTR expression fails to correct β-ENaC mouse lung disease
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Lung Cell Mol Physiol 302:L238-47. 2012
    ....
  2. ncbi request reprint In vivo microdialysis for determination of nasal liquid ion composition
    Barbara R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Am J Physiol Cell Physiol 282:C1423-31. 2002
    ..7 +/- 0.4 mM) was significantly less in normal than in CF mice (16.6 +/- 4 mM). IVMD accurately samples nasal liquid for ionic composition. The ionic composition of nasal liquid in the normal and CF mice is similar...
  3. ncbi request reprint Culture of murine nasal epithelia: model for cystic fibrosis
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, 7011 Thurston Bowles Bldg, CB 7248, Univ of North Carolina, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Lung Cell Mol Physiol 290:L270-7. 2006
    ..Culture of nasal epithelia from DeltaF508 mice will be particularly useful in testing drugs that allow DeltaF508 CFTR to traffic to the membrane...
  4. pmc SERCA pump inhibitors do not correct biosynthetic arrest of deltaF508 CFTR in cystic fibrosis
    Barbara R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, 7011 Thurston Bowles Bldg, CB 7248, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    Am J Respir Cell Mol Biol 34:355-63. 2006
    ....
  5. ncbi request reprint Olfactory epithelia exhibit progressive functional and morphological defects in CF mice
    Barbara R Grubb
    CF Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Cell Physiol 293:C574-83. 2007
    ..The CF mouse thus provides a novel model to examine the mechanisms of disease-associated loss of olfactory function...
  6. ncbi request reprint Alterations in airway ion transport in NKCC1-deficient mice
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
    Am J Physiol Cell Physiol 281:C615-23. 2001
    ..The absence of spontaneous airway disease or pathology in airways from the NKCC1-/- mice suggests that the NKCC1 mutant mice are able to compensate adequately for absence of the NKCC1 protein...
  7. ncbi request reprint Mucociliary transport determined by in vivo microdialysis in the airways of normal and CF mice
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, The Univ of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Lung Cell Mol Physiol 286:L588-95. 2004
    ..In conclusion, we have developed a versatile, simple in vivo method to measure MCT in both upper and lower airways of mice and larger animals...
  8. ncbi request reprint Intestinal ion transport in NKCC1-deficient mice
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
    Am J Physiol Gastrointest Liver Physiol 279:G707-18. 2000
    ....
  9. ncbi request reprint Ion transport across the normal and CF neonatal murine intestine
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    Am J Physiol 277:G167-74. 1999
    ....
  10. pmc Ion transport across CF and normal murine olfactory and ciliated epithelium
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, 7011 Thurston Bowles Bldg, CB 7248, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolia 27599 7248, USA
    Am J Physiol Cell Physiol 296:C1301-9. 2009
    ..Thus, while the murine CF nasal epithelium is a valuable model for CF studies, the bioelectrics are likely dominated by the signals from the OE, and assays of the nasopharynx may be more specific for studying the ciliated epithelium...
  11. ncbi request reprint Expression of CFTR from a ciliated cell-specific promoter is ineffective at correcting nasal potential difference in CF mice
    L E Ostrowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Gene Ther 14:1492-501. 2007
    ..However, expression of human CFTR in ciliated cells of the nasal epithelia failed to significantly change the nasal bioelectrics of the CF mice...
  12. ncbi request reprint The CF salt controversy: in vivo observations and therapeutic approaches
    R Tarran
    Cystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell 8:149-58. 2001
    ....
  13. ncbi request reprint Evidence for periciliary liquid layer depletion, not abnormal ion composition, in the pathogenesis of cystic fibrosis airways disease
    H Matsui
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 27599 7248, USA
    Cell 95:1005-15. 1998
    ..These data indicate that therapy for CF lung disease should not be directed at modulation of ionic composition, but rather at restoring volume (salt and water) on airway surfaces...
  14. pmc The relative roles of passive surface forces and active ion transport in the modulation of airway surface liquid volume and composition
    R Tarran
    Cystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 27599, USA
    J Gen Physiol 118:223-36. 2001
    ..These data favor the active ion transport/volume model hypothesis to describe ASL physiology...
  15. ncbi request reprint Cloning and functional characterization of two murine uridine nucleotide receptors reveal a potential target for correcting ion transport deficiency in cystic fibrosis gallbladder
    E R Lazarowski
    University of North Carolina School of Medicine, Department of Medicine, Cystic Fibrosis Pulmonary Research and Treatment Center, CB 7248, 7017 Thurston Bowles Building, Chapel Hill, NC 27599 7248, USA
    J Pharmacol Exp Ther 297:43-9. 2001
    ....
  16. ncbi request reprint Pathophysiology of gene-targeted mouse models for cystic fibrosis
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, The University of North Carolina at Chapel Hill, USA
    Physiol Rev 79:S193-214. 1999
    ..Reasons for these unexpected findings are discussed. This paper also reviews pharmacotherapeutic and gene therapeutic studies in the various mouse models...
  17. ncbi request reprint Effect of loss of P2Y(2) receptor gene expression on nucleotide regulation of murine epithelial Cl(-) transport
    V L Cressman
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 274:26461-8. 1999
    ..We conclude that the P2Y(2) receptor is the dominant P2Y purinoceptor that regulates airway epithelial ion transport, whereas other P2Y receptor subtypes are relatively more important in other nonrespiratory epithelia...
  18. ncbi request reprint Enhanced colonic Na+ absorption in cystic fibrosis mice versus normal mice
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, The University of North Carolina at Chapel Hill, 27599, USA
    Am J Physiol 272:G393-400. 1997
    ..Alternatively, other factors may be involved...
  19. ncbi request reprint Isolation and culture of airway epithelial cells from chronically infected human lungs
    S H Randell
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 27599 7248, USA
    In Vitro Cell Dev Biol Anim 37:480-9. 2001
    ..Tailored combinations of antibiotics at appropriate doses killed even multidrug-resistant bacteria. Thus, epithelial cells can usually be cultured from chronically infected CF airways...
  20. ncbi request reprint Bioelectric measurement of CFTR function in mice
    Barbara R Grubb
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC, USA
    Methods Mol Med 70:525-35. 2002
  21. ncbi request reprint Enhanced in vivo airway gene transfer via transient modification of host barrier properties with a surface-active agent
    D W Parsons
    Cystic Fibrosis Center, Department of Medicine, University of North Carolina at Chapel Hill, 27599, USA
    Hum Gene Ther 9:2661-72. 1998
    ..We conclude that the use of adjuvant surface-active and/or membrane-perturbing agents, synthetic or naturally derived, may provide a novel approach to enhancing the efficiency of adenoviral gene transfer...
  22. ncbi request reprint Intestinal physiology and pathology in gene-targeted mouse models of cystic fibrosis
    B R Grubb
    Cystic Fibrosis Pulmonary Research and Clinical Treatment Center, University of North Carolina, Chapel Hill 27599, USA
    Am J Physiol 273:G258-66. 1997
    ..This review describes the CF models generated and compares the murine defects in ion transport with observed abnormalities in the human CF intestine...
  23. ncbi request reprint Increased airway epithelial Na+ absorption produces cystic fibrosis-like lung disease in mice
    Marcus Mall
    Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, The University of North Carolina at Chapel Hill, 7011 Thurston Bowles Building, Chapel Hill, North Carolina 27599 7248, USA
    Nat Med 10:487-93. 2004
    ..We conclude that increasing airway Na(+) absorption initiates cystic fibrosis-like lung disease and produces a model for the study of the pathogenesis and therapy of this disease...
  24. pmc Conditional deletion of dnaic1 in a murine model of primary ciliary dyskinesia causes chronic rhinosinusitis
    Lawrence E Ostrowski
    The University of North Carolina at Chapel Hill School of Medicine, Cystic Fibrosis Pulmonary Research and Treatment Center, CB 7248, 6123A Thurston Bowles Bldg, Chapel Hill, NC 27599 7248, USA
    Am J Respir Cell Mol Biol 43:55-63. 2010
    ..This model will be useful for the study of the pathogenesis and treatment of PCD...
  25. pmc In vivo airway surface liquid Cl- analysis with solid-state electrodes
    Ray A Caldwell
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Gen Physiol 119:3-14. 2002
    ..3 +/- 7.1 mM, n = 5) and monkey (128.5 +/- 6.8 mM, n = 3). The measured [Cl-], in excess of 100 mM throughout all airway regions tested in multiple species, is consistent with the isotonic volume hypothesis to describe ASL physiology...
  26. ncbi request reprint Reduced three-dimensional motility in dehydrated airway mucus prevents neutrophil capture and killing bacteria on airway epithelial surfaces
    Hirotoshi Matsui
    Cystic Fibrosis Pulmonary Research and Treatment Center and Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    J Immunol 175:1090-9. 2005
    ..We conclude that concentrated ("thick") mucus inhibits neutrophil migration and killing and is a key component in the failure of defense against chronic airways infection in CF...
  27. pmc Nucleotide release provides a mechanism for airway surface liquid homeostasis
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina School of Medicine, 7017 Thurston Bowles Building, Chapel Hill, NC 72599, USA
    J Biol Chem 279:36855-64. 2004
    ..Our data reveal a complex pattern of nucleotides/nucleosides in ASL under resting conditions and suggest that adenosine may play a key role in regulating ASL volume homeostasis...
  28. pmc Airway and lung pathology due to mucosal surface dehydration in {beta}-epithelial Na+ channel-overexpressing mice: role of TNF-{alpha} and IL-4R{alpha} signaling, influence of neonatal development, and limited efficacy of glucocorticoid treatment
    Alessandra Livraghi
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 27599, USA
    J Immunol 182:4357-67. 2009
    ..Thus, manipulation of multiple pathways will likely be required to treat the complex pathogenesis caused by airway surface dehydration...
  29. pmc Human alveolar type II cells secrete and absorb liquid in response to local nucleotide signaling
    Peter F Bove
    Department of Medicine, Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 285:34939-49. 2010
    ..Collectively, our studies suggest that human AT2 cells in vitro have the capacity to absorb or secrete liquid in response to local alveolar conditions...
  30. ncbi request reprint Expression of the amino acid transporter ATB 0+ in lung: possible role in luminal protein removal
    Jennifer L Sloan
    Department of Cell and Molecular Physiology and the Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Am J Physiol Lung Cell Mol Physiol 284:L39-49. 2003
    ..We hypothesize that, by removing amino acids from the airway lumen, the transporter contributes to protein clearance and, by maintaining a low nutrient environment, plays a role in lung defense...
  31. pmc Abnormal surface liquid pH regulation by cultured cystic fibrosis bronchial epithelium
    Raymond D Coakley
    Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Proc Natl Acad Sci U S A 100:16083-8. 2003
    ....
  32. ncbi request reprint The cystic fibrosis transmembrane conductance regulator is regulated by a direct interaction with the protein phosphatase 2A
    William R Thelin
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 280:41512-20. 2005
    ..Furthermore, PP2A may be a clinically relevant drug target for CF, which should be considered in future studies...
  33. ncbi request reprint Extracellular nucleotides block bone mineralization in vitro: evidence for dual inhibitory mechanisms involving both P2Y2 receptors and pyrophosphate
    Isabel R Orriss
    Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, United Kingdom
    Endocrinology 148:4208-16. 2007
    ..Thus, a component of the profound inhibitory action of ATP and UTP on bone mineralization could be mediated directly by PPi, independently of P2 receptors...