Hasmik Grigoryan

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Covalent binding of the organophosphorus agent FP-biotin to tyrosine in eight proteins that have no active site serine
    Hasmik Grigoryan
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, United States
    Chem Biol Interact 180:492-8. 2009
  2. pmc Mass spectrometry identifies multiple organophosphorylated sites on tubulin
    Hasmik Grigoryan
    University of Nebraska Medical Center, Eppley Institute for Cancer Research, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicol Appl Pharmacol 240:149-58. 2009
  3. pmc Mass spectrometry identifies covalent binding of soman, sarin, chlorpyrifos oxon, diisopropyl fluorophosphate, and FP-biotin to tyrosines on tubulin: a potential mechanism of long term toxicity by organophosphorus agents
    Hasmik Grigoryan
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Chem Biol Interact 175:180-6. 2008
  4. pmc Mass spectral characterization of organophosphate-labeled, tyrosine-containing peptides: characteristic mass fragments and a new binding motif for organophosphates
    Lawrence M Schopfer
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 878:1297-311. 2010
  5. pmc Tyrosines of human and mouse transferrin covalently labeled by organophosphorus agents: a new motif for binding to proteins that have no active site serine
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Toxicol Sci 107:144-55. 2009
  6. pmc Mass spectral characterization of organophosphate-labeled lysine in peptides
    Hasmik Grigoryan
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA
    Anal Biochem 394:92-100. 2009
  7. pmc Pseudo-esterase activity of human albumin: slow turnover on tyrosine 411 and stable acetylation of 82 residues including 59 lysines
    Oksana Lockridge
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 283:22582-90. 2008
  8. pmc Nanoimages show disruption of tubulin polymerization by chlorpyrifos oxon: implications for neurotoxicity
    Hasmik Grigoryan
    University of Nebraska Medical Center, Eppley Institute for Cancer Research, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicol Appl Pharmacol 240:143-8. 2009

Collaborators

Detail Information

Publications8

  1. pmc Covalent binding of the organophosphorus agent FP-biotin to tyrosine in eight proteins that have no active site serine
    Hasmik Grigoryan
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, United States
    Chem Biol Interact 180:492-8. 2009
    ..Another application is in the search for biomarkers of organophosphorus agent exposure. Previous searches have been limited to serine hydrolases. Now proteins such as albumin and keratin can be considered...
  2. pmc Mass spectrometry identifies multiple organophosphorylated sites on tubulin
    Hasmik Grigoryan
    University of Nebraska Medical Center, Eppley Institute for Cancer Research, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicol Appl Pharmacol 240:149-58. 2009
    ..In conclusion seventeen tyrosines in tubulin have the potential to covalently bind chlorpyrifos oxon. These results will be useful when searching for OP-labeled tubulin in live animals...
  3. pmc Mass spectrometry identifies covalent binding of soman, sarin, chlorpyrifos oxon, diisopropyl fluorophosphate, and FP-biotin to tyrosines on tubulin: a potential mechanism of long term toxicity by organophosphorus agents
    Hasmik Grigoryan
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Chem Biol Interact 175:180-6. 2008
    ..It is concluded that OP bind covalently to tubulin, and that this binding could explain cognitive impairment associated with OP exposure...
  4. pmc Mass spectral characterization of organophosphate-labeled, tyrosine-containing peptides: characteristic mass fragments and a new binding motif for organophosphates
    Lawrence M Schopfer
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 878:1297-311. 2010
    ..Modified peptides are difficult to find when the OP bears no radiolabel and no tag. The characteristic MSMS fragment ions are valuable because they are identifiers for OP-tyrosine, independent of the peptide...
  5. pmc Tyrosines of human and mouse transferrin covalently labeled by organophosphorus agents: a new motif for binding to proteins that have no active site serine
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Toxicol Sci 107:144-55. 2009
    ..The extent to which tyrosine modification by OP can occur in vivo and the toxicological implications of such modifications require further investigation...
  6. pmc Mass spectral characterization of organophosphate-labeled lysine in peptides
    Hasmik Grigoryan
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA
    Anal Biochem 394:92-100. 2009
    ..0, 181.2, and 83.8amu. This new OP-binding motif to lysine suggests new directions to search for mechanisms of long-term effects of OP exposure and in the search for biomarkers of OP exposure...
  7. pmc Pseudo-esterase activity of human albumin: slow turnover on tyrosine 411 and stable acetylation of 82 residues including 59 lysines
    Oksana Lockridge
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 283:22582-90. 2008
    ..Acetylated lysines formed adducts that were even more stable. In conclusion, the pseudo-esterase activity of albumin is the result of irreversible acetylation of 82 residues and is not the result of turnover...
  8. pmc Nanoimages show disruption of tubulin polymerization by chlorpyrifos oxon: implications for neurotoxicity
    Hasmik Grigoryan
    University of Nebraska Medical Center, Eppley Institute for Cancer Research, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicol Appl Pharmacol 240:143-8. 2009
    ..In conclusion, chlorpyrifos oxon binding to tubulin disrupts tubulin polymerization. These results may lead to an understanding of the neurotoxicity of organophosphorus agents...