Jack Griffith

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Replication fork regression in repetitive DNAs
    Nicole Fouché
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, The University of North Carolina, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 34:6044-50. 2006
  2. pmc Details of ssDNA annealing revealed by an HSV-1 ICP8-ssDNA binary complex
    Gökhan Tolun
    Lineberger Comprehensive Cancer Center, and Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 41:5927-37. 2013
  3. ncbi request reprint Visualizing nucleic acids and their complexes using electron microscopy
    J D Griffith
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599 7295, USA
    Curr Opin Struct Biol 7:362-6. 1997
  4. ncbi request reprint Mammalian telomeres end in a large duplex loop
    J D Griffith
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill and Curriculum in Genetics and Molecular Biology, 27599 7295, USA
    Cell 97:503-14. 1999
  5. doi request reprint Discovery of abundant cellulose microfibers encased in 250 Ma Permian halite: a macromolecular target in the search for life on other planets
    Jack D Griffith
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Astrobiology 8:215-28. 2008
  6. pmc Remodeling of the human papillomavirus type 11 replication origin into discrete nucleoprotein particles and looped structures by the E2 protein
    Jeonggu Sim
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    J Mol Biol 375:1165-77. 2008
  7. ncbi request reprint Visualization of the annealing of complementary single-stranded DNA catalyzed by the herpes simplex virus type 1 ICP8 SSB/recombinase
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    J Mol Biol 355:911-22. 2006
  8. ncbi request reprint The basic domain of TRF2 directs binding to DNA junctions irrespective of the presence of TTAGGG repeats
    Nicole Fouché
    Lineberger Comprehensive Cancer Center and Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 281:37486-95. 2006
  9. ncbi request reprint Two-dimensional crystallization of herpes simplex virus type 1 single-stranded DNA-binding protein, ICP8, on a lipid monolayer
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Campus Box 7295, Chapel Hill, NC 27599 7295, USA
    Biochim Biophys Acta 1701:101-8. 2004
  10. pmc Unpacking a gel-forming mucin: a view of MUC5B organization after granular release
    Mehmet Kesimer
    1Department of Biochemistry and Biophysics and Cystic Fibrosis Pulmonary Research and Treatment Center, Chapel Hill, NC, USA
    Am J Physiol Lung Cell Mol Physiol 298:L15-22. 2010

Collaborators

Detail Information

Publications51

  1. pmc Replication fork regression in repetitive DNAs
    Nicole Fouché
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, The University of North Carolina, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 34:6044-50. 2006
    ....
  2. pmc Details of ssDNA annealing revealed by an HSV-1 ICP8-ssDNA binary complex
    Gökhan Tolun
    Lineberger Comprehensive Cancer Center, and Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 41:5927-37. 2013
    ..3 MDa. Fitting of the ICP8 crystal structure suggests a mechanism for the annealing reaction catalyzed by ICP8, which is most likely a general mechanism for protein-driven DNA annealing...
  3. ncbi request reprint Visualizing nucleic acids and their complexes using electron microscopy
    J D Griffith
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599 7295, USA
    Curr Opin Struct Biol 7:362-6. 1997
    ..The polymorphic, flexible, and irregular nature of these complexes presents particular problems in their analysis...
  4. ncbi request reprint Mammalian telomeres end in a large duplex loop
    J D Griffith
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill and Curriculum in Genetics and Molecular Biology, 27599 7295, USA
    Cell 97:503-14. 1999
    ..T loops may provide a general mechanism for the protection and replication of telomeres...
  5. doi request reprint Discovery of abundant cellulose microfibers encased in 250 Ma Permian halite: a macromolecular target in the search for life on other planets
    Jack D Griffith
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Astrobiology 8:215-28. 2008
    ..This discovery points to cellulose as an ideal macromolecular target in the search for life on other planets in our Solar System...
  6. pmc Remodeling of the human papillomavirus type 11 replication origin into discrete nucleoprotein particles and looped structures by the E2 protein
    Jeonggu Sim
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    J Mol Biol 375:1165-77. 2008
    ..The role of these structures in the modeling and regulation of the HPV-11 ori is discussed...
  7. ncbi request reprint Visualization of the annealing of complementary single-stranded DNA catalyzed by the herpes simplex virus type 1 ICP8 SSB/recombinase
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    J Mol Biol 355:911-22. 2006
    ..Mechanisms for how the coiled-coil structures facilitate annealing are discussed...
  8. ncbi request reprint The basic domain of TRF2 directs binding to DNA junctions irrespective of the presence of TTAGGG repeats
    Nicole Fouché
    Lineberger Comprehensive Cancer Center and Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 281:37486-95. 2006
    ..Junction-specific binding would also allow TRF2 to stabilize a strand invasion structure that is thought to exist at the strand invasion site of the t-loop...
  9. ncbi request reprint Two-dimensional crystallization of herpes simplex virus type 1 single-stranded DNA-binding protein, ICP8, on a lipid monolayer
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Campus Box 7295, Chapel Hill, NC 27599 7295, USA
    Biochim Biophys Acta 1701:101-8. 2004
    ..5 nm filament with a shallow 31.2 nm pitch were observed and reconstruction revealed a double-helical structure...
  10. pmc Unpacking a gel-forming mucin: a view of MUC5B organization after granular release
    Mehmet Kesimer
    1Department of Biochemistry and Biophysics and Cystic Fibrosis Pulmonary Research and Treatment Center, Chapel Hill, NC, USA
    Am J Physiol Lung Cell Mol Physiol 298:L15-22. 2010
    ....
  11. pmc Ring-shaped Rad51 paralog protein complexes bind Holliday junctions and replication forks as visualized by electron microscopy
    Sarah A Compton
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 285:13349-56. 2010
    ..This work provides the first EM visualization of BCDX2 and CX3 binding to Holliday junctions and forked DNAs and suggests the complexes form ring-shaped structures...
  12. pmc The Werner syndrome protein binds replication fork and holliday junction DNAs as an oligomer
    Sarah A Compton
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 283:24478-83. 2008
    ..Calculations of the molecular weight of full-length WRN revealed that, in solution, WRN exists predominantly as a dimer. However, WRN bound to DNA is larger; the mass is consistent with that of a tetramer...
  13. ncbi request reprint Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures
    Funda Sar
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 279:39289-95. 2004
    ....
  14. pmc Origin-specific unwinding of herpes simplex virus 1 DNA by the viral UL9 and ICP8 proteins: visualization of a specific preunwinding complex
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    Proc Natl Acad Sci U S A 100:898-903. 2003
    ..Unwinding proceeded until the acquisition of a high level of positive supertwists in the remaining duplex DNA inhibited further unwinding. Addition of topoisomerase I allowed further unwinding, opening >1 kb of DNA around oriS...
  15. pmc Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies
    PAULINE E CHUGH
    Lineberger Comprehensive Cancer Center, Program in Global Oncology, Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Pathog 9:e1003484. 2013
    ..These could represent candidate biomarkers of disease and may contribute to the paracrine phenotypes that are a characteristic of KS. ..
  16. pmc The Kaposi's sarcoma-associated herpesvirus ORF6 DNA binding protein forms long DNA-free helical protein filaments
    Sezgin Ozgur
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    J Struct Biol 174:37-43. 2011
    ..The possibility that these protein filaments generate a scaffold upon which viral DNA replication, recombination, and encapsidation occur in the infected cell nucleus is discussed...
  17. ncbi request reprint Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy
    Jack D Griffith
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 277:15233-6. 2002
    ..Therefore, our results demonstrate structural similarity between the checkpoint Rad complexes and the PCNA and RFC replication factors and thus provide further support for models proposing analogous functions for these complexes...
  18. ncbi request reprint Mapping subunit location on the Saccharomyces cerevisiae origin recognition complex free and bound to DNA using a novel nanoscale biopointer
    Paul D Chastain
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    J Biol Chem 279:36354-62. 2004
    ..This versatile, new approach to mapping protein structure has potential for many applications...
  19. ncbi request reprint Modulation of p53 binding to Holliday junctions and 3-cytosine bulges by phosphorylation events
    Deepa Subramanian
    Lineberger Comprehensive Cancer Center and Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Biochemistry 44:2536-44. 2005
    ..In all cases, phosphorylation of serine 392 occurs; however, reactivation also involves other residues. Together, the results show that p53 DNA binding activity is strongly regulated by the phosphorylation state of the protein...
  20. ncbi request reprint Architecture of the replication complex and DNA loops at the fork generated by the bacteriophage t4 proteins
    Paul D Chastain
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:21276-85. 2003
    ..These bobbins constitute a major portion of the mass of the full replication complex...
  21. pmc The bipolar filaments formed by herpes simplex virus type 1 SSB/recombination protein (ICP8) suggest a mechanism for DNA annealing
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, Department Microbiology and Immunology, University of North Carolina at Chapel Hill, 27517, USA
    J Mol Biol 386:273-9. 2009
    ....
  22. ncbi request reprint p53 Monitors replication fork regression by binding to "chickenfoot" intermediates
    Deepa Subramanian
    Lineberger Comprehensive Cancer Center and Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 280:42568-72. 2005
    ..Collectively, these results demonstrate the importance of sequence-independent binding in p53-mediated maintenance of genomic integrity...
  23. pmc Telomere loops and homologous recombination-dependent telomeric circles in a Kluyveromyces lactis telomere mutant strain
    Anthony J Cesare
    Corresonding author Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell Biol 28:20-9. 2008
    ..These results suggest that disruption of Rap1 results in elevated recombination at telomeres, leading to increased strand invasion of the 3' overhang within t-loop junctions and resolution of the t-loop junctions into free t-circles...
  24. ncbi request reprint p53 binds telomeric single strand overhangs and t-loop junctions in vitro
    Rachel M Stansel
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 277:11625-8. 2002
    ..These results suggest that p53 has an active role in telomere maintenance and structure through association with the t-loop junction...
  25. pmc Preferential binding of ATR protein to UV-damaged DNA
    Keziban Unsal-Kaçmaz
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 99:6673-8. 2002
    ..In addition, damaged DNA stimulates the kinase activity of ATR to a significantly higher level than undamaged DNA. Our data suggest that ATR may function as an initial sensor in the DNA damage checkpoint response...
  26. pmc Interactions between p53, hMSH2-hMSH6 and HMG I(Y) on Holliday junctions and bulged bases
    Deepa Subramanian
    Lineberger Comprehensive Cancer Center and Department of Microbiology and Immunology, CB 7295 Mason Farm Road, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Nucleic Acids Res 30:2427-34. 2002
    ..Together, these results demonstrate that p53 DNA binding activity is highly influenced by the presence of other proteins, some having a dominant effect while others have a negative effect...
  27. ncbi request reprint The Epstein-Barr virus polymerase accessory factor BMRF1 adopts a ring-shaped structure as visualized by electron microscopy
    Alexander M Makhov
    Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 279:40358-61. 2004
    ..These findings extend the theme of polymerase accessory factors adopting ring-shaped structures and provide an example in which the ring is significantly larger than any previously described sliding clamp...
  28. pmc Telomeric DNA in ALT cells is characterized by free telomeric circles and heterogeneous t-loops
    Anthony J Cesare
    Lineberger Comprehensive Cancer Center, Rm 11 119, CB 7295, Mason Farm Rd, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    Mol Cell Biol 24:9948-57. 2004
    ..Results presented here also indicate that expression of telomerase in GM847 cells does not affect t-loop or extrachromosomal circle formation...
  29. ncbi request reprint Xrcc3 and Nbs1 are required for the production of extrachromosomal telomeric circles in human alternative lengthening of telomere cells
    Sarah A Compton
    Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Mason Farm Road, Chapel Hill, NC 27599, USA
    Cancer Res 67:1513-9. 2007
    ..We show that Xrcc3 and Nbs1 are indeed required for the production of t circles in human ALT. However, these cells continue to proliferate in the absence of t circles, suggesting that they are not required for the survival of ALT cells...
  30. ncbi request reprint Electron microscopic visualization of telomerase from Euplotes aediculatus bound to a model telomere DNA
    Nicole Fouché
    Lineberger Comprehensive Cancer Center and Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 45:9624-31. 2006
    ..These data provide the first direct evidence that telomerase is a functional dimer and suggest that two telomerase ribonucleoprotein particles cooperate to elongate each Euplotes telomere in vivo...
  31. ncbi request reprint Telomere looping in P. sativum (common garden pea)
    Anthony J Cesare
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Plant J 36:271-9. 2003
    ..Some molecules had loops as large as 75 kb. These results show that the arrangement of telomeric DNA into loops occurs in higher plants...
  32. ncbi request reprint Regulation of the interaction of inosine monophosphate dehydrogenase with mycophenolic Acid by GTP
    YanShan Ji
    Department of Pharmacology and the Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 281:206-12. 2006
    ..We conclude that intracellular GTP acts as an antagonist to MPA by directly binding to IMPDH and reversing the conformational changes in the protein...
  33. ncbi request reprint Termination factor-mediated DNA loop between termination and initiation sites drives mitochondrial rRNA synthesis
    Miguel Martin
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 123:1227-40. 2005
    ..These results strongly suggest that, to satisfy the need for high rate of rDNA transcription, human mitochondrial rRNA synthesis involves mTERF-mediated rDNA looping that promotes recycling of the transcription machinery...
  34. ncbi request reprint Lagging strand synthesis in coordinated DNA synthesis by bacteriophage t7 replication proteins
    JoonSoo Lee
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA
    J Mol Biol 316:19-34. 2002
    ..In the absence of a primase recognition site on the minicircle template no lagging strand synthesis occurs. The size of the Okazaki fragments is not affected by the number of recognition sites on the template...
  35. ncbi request reprint Taz1 binding to a fission yeast model telomere: formation of telomeric loops and higher order structures
    Lubomir Tomaska
    Department of Genetics, Comenius University, Faculty of Natural Sciences, Mlynska dolina, 842 15 Bratislava, Slovakia
    J Biol Chem 279:50764-72. 2004
    ..Based on these in vitro results we discuss possible general implications for fission yeast telomere dynamics...
  36. ncbi request reprint Catalysis of strand exchange by the HSV-1 UL12 and ICP8 proteins: potent ICP8 recombinase activity is revealed upon resection of dsDNA substrate by nuclease
    Nina B Reuven
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington 06030 3205, USA
    J Mol Biol 342:57-71. 2004
    ..In this reaction, the resection of a DNA end by the nuclease is required to reveal homologous sequences capable of being paired by ICP8. This study further illustrates the complexity of the multi-functional ICP8 protein...
  37. pmc Synapsis of DNA ends by DNA-dependent protein kinase
    Lisa G DeFazio
    Departments of Medicine and Biochemistry, Stanford University, Stanford, CA 94305, USA
    EMBO J 21:3192-200. 2002
    ..Our results suggest that DNA-PK(CS) brings DNA ends together and then undergoes activation of its kinase, presumably to regulate subsequent steps for processing and ligation of the ends...
  38. ncbi request reprint Amplification of telomeric arrays via rolling-circle mechanism
    Jozef Nosek
    Department of Biochemistry, Mlynska dolina CH 1, Comenius University, 842 15 Bratislava, Slovakia
    J Biol Chem 280:10840-5. 2005
    ....
  39. ncbi request reprint Sticky DNA, a long GAA.GAA.TTC triplex that is formed intramolecularly, in the sequence of intron 1 of the frataxin gene
    Alexandre A Vetcher
    Center for Genome Research, Institute of Biosciences and Technology, Texas A and M University, Texas Medical Center, Houston, Texas 77030 3303, USA
    J Biol Chem 277:39217-27. 2002
    ..Hence, these results give new insights into our understanding of the capacity of sticky DNA to inhibit transcription and thereby reduce the level of frataxin protein as related to the etiology of Friedreich's ataxia...
  40. doi request reprint C. elegans telomeres contain G-strand and C-strand overhangs that are bound by distinct proteins
    Marcela Raices
    The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 132:745-57. 2008
    ..Our results suggest that C. elegans telomere homeostasis relies on a novel mechanism that involves 5' and 3' single-stranded termini...
  41. doi request reprint Tethering telomeric double- and single-stranded DNA-binding proteins inhibits telomere elongation
    Katherine T Etheridge
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 283:6935-41. 2008
    ..We speculate that such a protein bridge between dsDNA and ssDNA may inhibit telomerase access, promoting telomere shortening...
  42. pmc Chaperone proteins abrogate inhibition of the human papillomavirus (HPV) E1 replicative helicase by the HPV E2 protein
    Biing Yuan Lin
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 35294 0005, USA
    Mol Cell Biol 22:6592-604. 2002
    ..An E1 mutation in the ATP binding site caused deficient binding and unwinding of origin DNA, indicating the importance of ATP binding in efficient helicase assembly on the origin...
  43. ncbi request reprint The carboxyl-terminal domain of bacteriophage T7 single-stranded DNA-binding protein modulates DNA binding and interaction with T7 DNA polymerase
    Zheng Guo He
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:29538-45. 2003
    ..The biochemical and genetic data support a model in which the carboxyl-terminal tail modulates DNA binding and mediates essential interactions with T7 DNA polymerase...
  44. ncbi request reprint A single-stranded DNA-binding protein of bacteriophage T7 defective in DNA annealing
    Lisa F Rezende
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:29098-105. 2003
    ..Gene 2.5 protein-R82C condenses single-stranded M13 DNA in a manner similar to wild-type protein when viewed by electron microscopy...
  45. ncbi request reprint Architecture of the bacteriophage T4 replication complex revealed with nanoscale biopointers
    Nancy G Nossal
    Laboratory of Molecular and Cellular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0830, USA
    J Biol Chem 282:1098-108. 2007
    ..Unexpectedly, we found that 59 helicase-loading protein remains on the fork after loading the helicase and is present on molecules with extensive replication...
  46. pmc Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro
    Vladimir P Bermudez
    Program in Molecular Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:1633-8. 2003
    ..Rad17-RFC binds to nicked circular, gapped, and primed DNA and recruits the 9-1-1 complex in an ATP-dependent manner. Electron microscopic analyses of the reaction products indicate that the 9-1-1 ring is clamped around the DNA...
  47. pmc Recombination at long mutant telomeres produces tiny single- and double-stranded telomeric circles
    Cindy Groff-Vindman
    University of Georgia at Athens, Department of Genetics, Room C318, Life Sciences Building, Athens, GA 30602, USA
    Mol Cell Biol 25:4406-12. 2005
    ..The very small circles seen here are both predicted products of telomere rapid deletion, a process observed in both human and yeast cells, and predicted templates for roll-and-spread RTE...
  48. ncbi request reprint The processing of Holliday junctions by BLM and WRN helicases is regulated by p53
    Qin Yang
    Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:31980-7. 2002
    ..Our results are consistent with a novel mechanism for p53-mediated regulation of DNA recombinational repair that involves p53 post-translational modifications and functional protein-protein interactions with BLM and WRN DNA helicases...
  49. ncbi request reprint Differential single-stranded DNA binding properties of the paralogous SsbA and SsbB proteins from Streptococcus pneumoniae
    Diane E Grove
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 280:11067-73. 2005
    ..These results represent the first analysis of paralogous SSB proteins from any bacterial species and provide a foundation for further investigations into the biological roles of these proteins...
  50. pmc Role of p38 in replication of Trypanosoma brucei kinetoplast DNA
    Beiyu Liu
    Department of Biological Chemistry, Johns Hopkins Medical School, 725 N Wolfe St, Baltimore, MD 21205, USA
    Mol Cell Biol 26:5382-93. 2006
    ..p38 binds to minicircle sequences within the replication origin. We conclude that cells with RNAi-induced loss of p38 cannot initiate minicircle replication, although they can extensively unwind free minicircles...
  51. pmc Mechanism of replication-coupled DNA interstrand crosslink repair
    Markus Raschle
    Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Boston, MA 02115, USA
    Cell 134:969-80. 2008
    ..Ultimately, a significant portion of the input DNA is fully repaired, but not if DNA replication is blocked. Our experiments establish a mechanism for ICL repair that reveals how this process is coupled to DNA replication...

Research Grants31

  1. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 2000
    ..abstract_text> ..
  2. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 2001
    ..abstract_text> ..
  3. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 2004
    ..These studies will be extended to the more complex E. coli system to provide a structural understanding of the E. coli replisome and location of the individual protein components. ..
  4. DYNAMICS OF DNA LOOPING AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 1999
    ..Loops of DNA at the replication fork were visualized. Understanding looping and how loops grow and reform with Okazaki fragment synthesis is the goal of this project. ..
  5. Nucleoprotein structures formed at sites of DNA damage
    Jack Griffith; Fiscal Year: 2006
    ..Each system offers a unique window into basic questions of DNA protein remodeling at sites of damage and telomeres and information garnered from one study is immediately applied to the others. ..
  6. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 2007
    ..These studies will be extended to the more complex E. coli system to provide a structural understanding of the E. coli replisome and location of the individual protein components. ..
  7. Nucleoprotein structures formed at sites of DNA damage
    Jack Griffith; Fiscal Year: 2007
    ..Each system offers a unique window into basic questions of DNA protein remodeling at sites of damage and telomeres and information garnered from one study is immediately applied to the others. ..
  8. Nucleoprotein structures formed at sites of DNA damage
    Jack Griffith; Fiscal Year: 2009
    ..Each system offers a unique window into basic questions of DNA protein remodeling at sites of damage and telomeres and information garnered from one study is immediately applied to the others. ..
  9. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 2005
    ..These studies will be extended to the more complex E. coli system to provide a structural understanding of the E. coli replisome and location of the individual protein components. ..
  10. Nucleoprotein structures formed at sites of DNA damage
    Jack Griffith; Fiscal Year: 2005
    ..Each system offers a unique window into basic questions of DNA protein remodeling at sites of damage and telomeres and information garnered from one study is immediately applied to the others. ..
  11. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 2006
    ..These studies will be extended to the more complex E. coli system to provide a structural understanding of the E. coli replisome and location of the individual protein components. ..
  12. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 2009
    ....
  13. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 2003
    ..abstract_text> ..
  14. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack Griffith; Fiscal Year: 2002
    ..abstract_text> ..
  15. Purchase of Technai 12 TEM/STEM Electron Microscope
    Jack Griffith; Fiscal Year: 2002
    ..This facility under the direction of Dr. Jack Griffith serves NIH funded investigators both at UNC and across the US in need of the visualization of their nucleic ..
  16. HOMOLOGOUS RECOMBINATION: ITS MOLECULAR MECHANICS
    Jack Griffith; Fiscal Year: 1992
    ..A novel drug column will be used to search for new DNA recombination and repair proteins from eukaryotic cells...
  17. HOMOLOGOUS RECOMBINATION: ITS MOLECULAR CYTOLOGY
    Jack Griffith; Fiscal Year: 1990
    ..The uvsX protein of bacteriophage T4 appears analagous to RecA protein, and parallel studies with it will be initiated in a effort to begin to establish the rules of homologous recombination common to all cells...
  18. HOMOLOGOUS RECOMBINATION: ITS MOLECULAR MECHANICS
    Jack Griffith; Fiscal Year: 1993
    ..A novel drug column will be used to search for new DNA recombination and repair proteins from eukaryotic cells...
  19. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack D Griffith; Fiscal Year: 2010
    ....