Joshua C Grieger

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. ncbi request reprint Adeno-associated virus as a gene therapy vector: vector development, production and clinical applications
    Joshua C Grieger
    University of North Carolina Gene Therapy Center, Chapel Hill 27599, USA
    Adv Biochem Eng Biotechnol 99:119-45. 2005
  2. ncbi request reprint Production and characterization of adeno-associated viral vectors
    Joshua C Grieger
    Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Protoc 1:1412-28. 2006
  3. pmc Packaging capacity of adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps
    Joshua C Grieger
    Curriculm in Genetics and Molecular Biology and Gene Therapy Center, University of North Carolina at Chapel Hill, 27599 7352, USA
    J Virol 79:9933-44. 2005
  4. pmc Separate basic region motifs within the adeno-associated virus capsid proteins are essential for infectivity and assembly
    Joshua C Grieger
    Curriculum in Genetics and Molecular Biology, Gene Therapy Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7352, USA
    J Virol 80:5199-210. 2006
  5. pmc Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno-associated virus serotypes
    Zhijian Wu
    Gene Therapy Center, 7113 Thurston Building, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7352, USA
    J Virol 80:11393-7. 2006
  6. pmc Surface-exposed adeno-associated virus Vp1-NLS capsid fusion protein rescues infectivity of noninfectious wild-type Vp2/Vp3 and Vp3-only capsids but not that of fivefold pore mutant virions
    Joshua C Grieger
    Gene Therapy Center, University of North Carolina at Chapel Hill, 7119 Thurston Bowles, CB 7352, Chapel Hill, NC 27599 7352, USA
    J Virol 81:7833-43. 2007

Collaborators

  • Zhijian Wu
  • Aravind Asokan
  • R Jude Samulski
  • Mavis Agbandje-McKenna
  • Lakshmanan Govindasamy

Detail Information

Publications6

  1. ncbi request reprint Adeno-associated virus as a gene therapy vector: vector development, production and clinical applications
    Joshua C Grieger
    University of North Carolina Gene Therapy Center, Chapel Hill 27599, USA
    Adv Biochem Eng Biotechnol 99:119-45. 2005
    ..In this chapter, we will provide a comprehensive overview of the recent advances in rAAV vector production and purification, vector development, and clinical applications...
  2. ncbi request reprint Production and characterization of adeno-associated viral vectors
    Joshua C Grieger
    Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Protoc 1:1412-28. 2006
    ..This review provides an up-to-date, detailed description of essential methods such as production, purification and titering and their application to characterize current AAV vectors for preclinical and clinical use...
  3. pmc Packaging capacity of adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps
    Joshua C Grieger
    Curriculm in Genetics and Molecular Biology and Gene Therapy Center, University of North Carolina at Chapel Hill, 27599 7352, USA
    J Virol 79:9933-44. 2005
    ..0 kb but the larger genome-containing virions are preferentially degraded by the proteasome and that this block can be overcome by the addition of proteasome inhibitors...
  4. pmc Separate basic region motifs within the adeno-associated virus capsid proteins are essential for infectivity and assembly
    Joshua C Grieger
    Curriculum in Genetics and Molecular Biology, Gene Therapy Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7352, USA
    J Virol 80:5199-210. 2006
    ..This study establishes the importance of two BR motifs on the AAV2 capsid that are essential for infectivity and virion assembly...
  5. pmc Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno-associated virus serotypes
    Zhijian Wu
    Gene Therapy Center, 7113 Thurston Building, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7352, USA
    J Virol 80:11393-7. 2006
    ....
  6. pmc Surface-exposed adeno-associated virus Vp1-NLS capsid fusion protein rescues infectivity of noninfectious wild-type Vp2/Vp3 and Vp3-only capsids but not that of fivefold pore mutant virions
    Joshua C Grieger
    Gene Therapy Center, University of North Carolina at Chapel Hill, 7119 Thurston Bowles, CB 7352, Chapel Hill, NC 27599 7352, USA
    J Virol 81:7833-43. 2007
    ....