JESSE GREGORY

Summary

Affiliation: University of Florida
Country: USA

Publications

  1. ncbi request reprint Folate biofortification in food plants
    Samir Bekaert
    Department of Molecular Genetics, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Trends Plant Sci 13:28-35. 2008
  2. pmc Metabolomic analysis reveals extended metabolic consequences of marginal vitamin B-6 deficiency in healthy human subjects
    Jesse F Gregory
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida, United States of America
    PLoS ONE 8:e63544. 2013
  3. pmc Vitamin B-6 restriction impairs fatty acid synthesis in cultured human hepatoma (HepG2) cells
    Mei Zhao
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    Am J Physiol Endocrinol Metab 304:E342-51. 2013
  4. ncbi request reprint Primed, constant infusion with [2H3]serine allows in vivo kinetic measurement of serine turnover, homocysteine remethylation, and transsulfuration processes in human one-carbon metabolism
    J F Gregory
    Department of Nutritional Sciences, University of California Berkeley, Berkeley, CA, USA
    Am J Clin Nutr 72:1535-41. 2000
  5. ncbi request reprint In vivo kinetics of folate metabolism
    Jesse F Gregory
    Food Science and Human Nutrition Department, University of Florida, P O Box 110370, Gainesville, Florida 32611 0370, USA
    Annu Rev Nutr 22:199-220. 2002
  6. ncbi request reprint Kinetics of folate turnover in pregnant women (second trimester) and nonpregnant controls during folic acid supplementation: stable-isotopic labeling of plasma folate, urinary folate and folate catabolites shows subtle effects of pregnancy on turnover of
    J F Gregory
    Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611 0370, USA
    J Nutr 131:1928-37. 2001
  7. ncbi request reprint Case study: folate bioavailability
    J F Gregory
    Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida 32611 0370, USA
    J Nutr 131:1376S-82S. 2001
  8. ncbi request reprint Homocysteine synthesis is elevated but total remethylation is unchanged by the methylenetetrahydrofolate reductase 677C->T polymorphism and by dietary folate restriction in young women
    Steven R Davis
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, College of Medicine, University of Florida, Gainesville 32611 0370, USA
    J Nutr 135:1045-50. 2005
  9. ncbi request reprint Methionine synthase reductase 66A->G polymorphism is associated with increased plasma homocysteine concentration when combined with the homozygous methylenetetrahydrofolate reductase 677C->T variant
    Jaimie D Vaughn
    Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA
    J Nutr 134:2985-90. 2004
  10. pmc DNA digestion to deoxyribonucleoside: a simplified one-step procedure
    Eoin P Quinlivan
    Biomedical Mass Spectrometry Laboratory, General Clinical Research Center, University of Florida, Gainesville, FL 32611, USA
    Anal Biochem 373:383-5. 2008

Research Grants

Collaborators

Detail Information

Publications64

  1. ncbi request reprint Folate biofortification in food plants
    Samir Bekaert
    Department of Molecular Genetics, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Trends Plant Sci 13:28-35. 2008
    ..Here, we review the current status of investigations in the field of folate enhancement on the eve of a new era in food fortification...
  2. pmc Metabolomic analysis reveals extended metabolic consequences of marginal vitamin B-6 deficiency in healthy human subjects
    Jesse F Gregory
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida, United States of America
    PLoS ONE 8:e63544. 2013
    ..These findings demonstrate that marginal vitamin B-6 deficiency has widespread metabolic perturbations and illustrate the utility of metabolomics in evaluating complex effects of altered vitamin B-6 intake...
  3. pmc Vitamin B-6 restriction impairs fatty acid synthesis in cultured human hepatoma (HepG2) cells
    Mei Zhao
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    Am J Physiol Endocrinol Metab 304:E342-51. 2013
    ..These results and observations of changes in human plasma fatty acid profiles caused by vitamin B-6 restriction suggest a mechanism by which vitamin B-6 inadequacy influences the cardiovascular risk...
  4. ncbi request reprint Primed, constant infusion with [2H3]serine allows in vivo kinetic measurement of serine turnover, homocysteine remethylation, and transsulfuration processes in human one-carbon metabolism
    J F Gregory
    Department of Nutritional Sciences, University of California Berkeley, Berkeley, CA, USA
    Am J Clin Nutr 72:1535-41. 2000
    ..One-carbon metabolism involves both mitochondrial and cytosolic forms of folate-dependent enzymes in mammalian cells, but few in vivo data exist to characterize the biochemical processes involved...
  5. ncbi request reprint In vivo kinetics of folate metabolism
    Jesse F Gregory
    Food Science and Human Nutrition Department, University of Florida, P O Box 110370, Gainesville, Florida 32611 0370, USA
    Annu Rev Nutr 22:199-220. 2002
    ..This review considers the strengths and weaknesses of various approaches to the study of folate kinetics and resulting data, followed by a summary and interpretation of existing data...
  6. ncbi request reprint Kinetics of folate turnover in pregnant women (second trimester) and nonpregnant controls during folic acid supplementation: stable-isotopic labeling of plasma folate, urinary folate and folate catabolites shows subtle effects of pregnancy on turnover of
    J F Gregory
    Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611 0370, USA
    J Nutr 131:1928-37. 2001
    ....
  7. ncbi request reprint Case study: folate bioavailability
    J F Gregory
    Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida 32611 0370, USA
    J Nutr 131:1376S-82S. 2001
    ....
  8. ncbi request reprint Homocysteine synthesis is elevated but total remethylation is unchanged by the methylenetetrahydrofolate reductase 677C->T polymorphism and by dietary folate restriction in young women
    Steven R Davis
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, College of Medicine, University of Florida, Gainesville 32611 0370, USA
    J Nutr 135:1045-50. 2005
    ..In conclusion, elevated homocysteine synthesis is a cause of mild hyperhomocysteinemia in women with marginal folate status, particularly those with the MTHFR 677 T/T genotype...
  9. ncbi request reprint Methionine synthase reductase 66A->G polymorphism is associated with increased plasma homocysteine concentration when combined with the homozygous methylenetetrahydrofolate reductase 677C->T variant
    Jaimie D Vaughn
    Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA
    J Nutr 134:2985-90. 2004
    ....
  10. pmc DNA digestion to deoxyribonucleoside: a simplified one-step procedure
    Eoin P Quinlivan
    Biomedical Mass Spectrometry Laboratory, General Clinical Research Center, University of Florida, Gainesville, FL 32611, USA
    Anal Biochem 373:383-5. 2008
    ..The new protocol is fully compatible with LC-MS/MS and gives similar recoveries for all five major deoxyribonucleosides when compared to the older protocol...
  11. ncbi request reprint 5-Formyltetrahydrofolate is an inhibitory but well tolerated metabolite in Arabidopsis leaves
    Aymeric Goyer
    Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 280:26137-42. 2005
    ..However, the near-normal growth of the mutant shows that even exceptionally high 5-CHO-THF levels do not much affect fluxes through SHMT or any other folate-dependent reaction, i.e. that 5-CHO-THF is well tolerated in plants...
  12. pmc DNA methylation determination by liquid chromatography-tandem mass spectrometry using novel biosynthetic [U-15N]deoxycytidine and [U-15N]methyldeoxycytidine internal standards
    Eoin P Quinlivan
    Biomedical Mass Spectrometry Laboratory, General Clinical Research Center and Food Science and Human Nutrition Department, University of Florida, Gainesville, FL, USA
    Nucleic Acids Res 36:e119. 2008
    ..Inter- and intraassay variation for the assay (%RSD, n = 6) was <2.5%...
  13. ncbi request reprint Characterization of the folate salvage enzyme p-aminobenzoylglutamate hydrolase in plants
    Gale G Bozzo
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, United States
    Phytochemistry 69:29-37. 2008
    ..The finding that Arabidopsis has folate-hydrolyzing activity points to an enzymatic component of folate degradation in plants...
  14. ncbi request reprint Dietary vitamin B-6 restriction does not alter rates of homocysteine remethylation or synthesis in healthy young women and men
    Steven R Davis
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611 0370, USA
    Am J Clin Nutr 81:648-55. 2005
    ..The effects of vitamin B-6 status on steady-state kinetics of homocysteine metabolism in humans are unclear...
  15. ncbi request reprint The methylenetetrahydrofolate reductase 677C->T polymorphism and dietary folate restriction affect plasma one-carbon metabolites and red blood cell folate concentrations and distribution in women
    Steven R Davis
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, College of Medicine, University of Florida, Gainesville, FL 32611 0370, USA
    J Nutr 135:1040-4. 2005
    ....
  16. ncbi request reprint Folate deprivation reduces homocysteine remethylation in a human intestinal epithelial cell culture model: role of serine in one-carbon donation
    Justin H Townsend
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    Am J Physiol Gastrointest Liver Physiol 286:G588-95. 2004
    ..Remethylation is reduced as a result of folate restriction in this mucosal cell model, and one-carbon sources other than the three carbon of serine contribute to remethylation under this condition...
  17. ncbi request reprint Vitamin B-12 status is inversely associated with plasma homocysteine in young women with C677T and/or A1298C methylenetetrahydrofolate reductase polymorphisms
    Lynn B Bailey
    Food Science and Human Nutrition Department, and Department of Statistics, University of Florida, Gainesville 32611, USA
    J Nutr 132:1872-8. 2002
    ..These data suggest that enhancing vitamin B-12 status may significantly decrease homocysteine in young women with C677T and/or A1298C MTHFR polymorphisms, even when vitamin B-12 concentrations are within the normal range...
  18. ncbi request reprint Synthesis and turnover of folates in plants
    Andrew D Hanson
    Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
    Curr Opin Plant Biol 5:244-9. 2002
    ..Moreover, new analytical advances will help to elucidate plant folate turnover and transport, which are practically unexplored...
  19. ncbi request reprint Tracer-derived total and folate-dependent homocysteine remethylation and synthesis rates in humans indicate that serine is the main one-carbon donor
    Steven R Davis
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Science, University of Florida, Gainesville, Florida 32611 0370, USA
    Am J Physiol Endocrinol Metab 286:E272-9. 2004
    ..Our dual-tracer procedure is well suited to measure the effects of nutrient deficiencies, genetic polymorphisms, and other metabolic perturbations on homocysteine synthesis and total and folate-dependent homocysteine remethylation...
  20. ncbi request reprint Folate catabolite excretion is responsive to changes in dietary folate intake in elderly women
    Judith M Wolfe
    Food Science and Human Nutrition Department, College of Agricultural and Life Sciences, University of Florida, Gainesville 32611, USA
    Am J Clin Nutr 77:919-23. 2003
    ..We hypothesize that total pABG (ApABG + pABG) excretion parallels both the mass of body folate pools from which these catabolites originate and the folate-status indicators...
  21. ncbi request reprint Evidence for folate-salvage reactions in plants
    Giuseppe Orsomando
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
    Plant J 46:426-35. 2006
    ..We conclude that plants are excellent organisms in which to explore the biochemistry of folate salvage...
  22. pmc Folate biofortification of tomato fruit
    Rocio I Díaz De La Garza
    Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 104:4218-22. 2007
    ..The folate levels we achieved provide the complete adult daily requirement in less than one standard serving...
  23. ncbi request reprint Plant gamma-glutamyl hydrolases and folate polyglutamates: characterization, compartmentation, and co-occurrence in vacuoles
    Giuseppe Orsomando
    Horticultural Sciences and Food Science Department, University of Florida, Gainesville, FL 32611, USA
    J Biol Chem 280:28877-84. 2005
    ..These data point to a hitherto unsuspected role for vacuoles in folate storage. Furthermore, the paradoxical co-occurrence of GGH and folylpolyglutamates in vacuoles implies that the polyglutamates are somehow protected from GGH attack...
  24. ncbi request reprint Methylenetetrahydrofolate reductase 677C->T polymorphism and folate status affect one-carbon incorporation into human DNA deoxynucleosides
    Eoin P Quinlivan
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, College of Medicine, University of Florida, Gainesville, FL 32611, USA
    J Nutr 135:389-96. 2005
    ..These findings support the feasibility of further assessment of factors affecting deoxynucleotide synthesis and DNA methylation in human 1-carbon metabolism...
  25. ncbi request reprint A nudix enzyme removes pyrophosphate from dihydroneopterin triphosphate in the folate synthesis pathway of bacteria and plants
    Sebastian M J Klaus
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
    J Biol Chem 280:5274-80. 2005
    ..and Kraszewska, E. (2002) J. Biol. Chem. 277, 50482-50486). However, we found that this activity is negligible at physiological levels of Mn(2+) and that, with 1 mM Mg(2+), AtNUDT1 prefers DHNTP and (deoxy) nucleoside triphosphates...
  26. ncbi request reprint Folate status response to controlled folate intake is affected by the methylenetetrahydrofolate reductase 677C-->T polymorphism in young women
    Karla P Shelnutt
    Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA
    J Nutr 133:4107-11. 2003
    ..These results may be important when evaluating the impact of the MTHFR 677C-->T polymorphism in countries in which low folate diets are chronically consumed...
  27. ncbi request reprint Methylenetetrahydrofolate reductase 677C-->T polymorphism affects DNA methylation in response to controlled folate intake in young women
    Karla P Shelnutt
    Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611, USA
    J Nutr Biochem 15:554-60. 2004
    ..The response to folate repletion suggests that following folate depletion women with the MTHFR 677 TT genotype have a greater increase in DNA methylation with folate repletion than women with the CC genotype...
  28. pmc Identification of transport-critical residues in a folate transporter from the folate-biopterin transporter (FBT) family
    Aymerick Eudes
    Horticultural Sciences, Human Nutrition Department, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 285:2867-75. 2010
    ..coli. Such negative data could reflect roles in transport of other substrates. These studies provide the first insights into the native structure and catalytic mechanism of FBT family carriers...
  29. pmc Folate synthesis in plants: the p-aminobenzoate branch is initiated by a bifunctional PabA-PabB protein that is targeted to plastids
    Gilles J C Basset
    Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 101:1496-501. 2004
    ..In the pericarp of tomato fruit, the PabA-PabB mRNA level fell drastically as ripening advanced, but there was no fall in total PABA content, which stayed between 0.7 and 2.3 nmol.g(-1) fresh weight...
  30. ncbi request reprint Uptake, hydrolysis, and metabolism of pyridoxine-5'-beta-D-glucoside in Caco-2 cells
    Amy D Mackey
    Food Science and Human Nutrition Department, University of Florida, Gainesville, FL, USA
    J Nutr 134:842-6. 2004
    ..The presence of lactose in this in vitro model of intestinal uptake reduced the enzymatic hydrolysis of PNG by lactase...
  31. ncbi request reprint Higher plant plastids and cyanobacteria have folate carriers related to those of trypanosomatids
    Sebastian M J Klaus
    Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 280:38457-63. 2005
    ..These findings establish conservation of function among folate and biopterin transporter family proteins from three kingdoms of life...
  32. pmc Folate synthesis in plants: the first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I
    Gilles Basset
    Horticultural Sciences Department and Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 99:12489-94. 2002
    ..As ripening advances, GCHI expression declines sharply, and folate content drops, suggesting that folate synthesis fails to keep pace with turnover...
  33. pmc Pterin and folate salvage. Plants and Escherichia coli lack capacity to reduce oxidized pterins
    Alexandre Noiriel
    Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 143:1101-9. 2007
    ..If it is lacking, folate precursors or breakdown products that become oxidized will permanently exit the metabolically active pterin pool...
  34. ncbi request reprint The folate precursor p-aminobenzoate is reversibly converted to its glucose ester in the plant cytosol
    Eoin P Quinlivan
    Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 278:20731-7. 2003
    ..Esterification of pABA may eliminate such errant behavior and provide a readily reclaimable storage form of pABA as well as a substrate for membrane transporters...
  35. pmc Metabolism of the folate precursor p-aminobenzoate in plants: glucose ester formation and vacuolar storage
    Aymerick Eudes
    Departments of Horticultural Sciences and Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611, USA
    J Biol Chem 283:15451-9. 2008
    ..In total, these data and the fact that isolated mitochondria did not take up [(3)H]pABA-Glc, suggest that the glucose ester represents a storage form of pABA that does not contribute directly to folate synthesis...
  36. ncbi request reprint Folate synthesis in plants: the last step of the p-aminobenzoate branch is catalyzed by a plastidial aminodeoxychorismate lyase
    Gilles J C Basset
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
    Plant J 40:453-61. 2004
    ..As shown previously for the ADC synthase transcript, the level of ADC lyase mRNA in the pericarp of tomato fruit falls sharply as ripening advances, suggesting that the expression of these two enzymes is coregulated...
  37. ncbi request reprint Hydrolytic activity toward pyridoxine-5'-beta-D-glucoside in rat intestinal mucosa is not increased by vitamin B-6 deficiency: effect of basal diet composition and pyridoxine intake
    Amy D Mackey
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA
    J Nutr 133:1362-7. 2003
    ..In contrast to previous results from this laboratory, intestinal hydrolytic activity toward PNG did not increase in vitamin B-6-deficient rats...
  38. pmc Moderate dietary vitamin B-6 restriction raises plasma glycine and cystathionine concentrations while minimally affecting the rates of glycine turnover and glycine cleavage in healthy men and women
    Yvonne Lamers
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA
    J Nutr 139:452-60. 2009
    ..We conclude that the in vivo GCS and SHMT reactions are quite resilient to the effects of marginal vitamin B-6 deficiency, presumably through a compensatory effect of increasing substrate concentration...
  39. ncbi request reprint Enzymatic hydrolysis of pyridoxine-5'-beta-D-glucoside is catalyzed by intestinal lactase-phlorizin hydrolase
    Amy D Mackey
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA
    J Biol Chem 277:26858-64. 2002
    ..These results indicate that LPH may play an important role in the bioavailability of PNG, but further characterization is needed to assess its physiological function...
  40. pmc A role for tetrahydrofolates in the metabolism of iron-sulfur clusters in all domains of life
    Jeffrey C Waller
    Horticultural Sciences, Food Science and Human Nutrition, Microbiology and Cell Science Departments, and Department of Biochemistry and Molecular Biology and National High Magnetic Field Laboratory, and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 107:10412-7. 2010
    ..Thus COG0354 proteins have an ancient, conserved, folate-dependent function in the activity of certain Fe/S cluster enzymes...
  41. ncbi request reprint Glycine turnover and decarboxylation rate quantified in healthy men and women using primed, constant infusions of [1,2-(13)C2]glycine and [(2)H3]leucine
    Yvonne Lamers
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    J Nutr 137:2647-52. 2007
    ..GCS is responsible for a substantial proportion of whole body glycine flux and constitutes a major route for the generation of 1-carbon units...
  42. ncbi request reprint Vitamin B-6 deficiency suppresses the hepatic transsulfuration pathway but increases glutathione concentration in rats fed AIN-76A or AIN-93G diets
    Carolina P Lima
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA
    J Nutr 136:2141-7. 2006
    ..In spite of these effects, hepatic glutathione concentration increased in vitamin B-6 deficiency. These results suggest that vitamin B-6-dependent changes in transsulfuration do not limit hepatic glutathione production...
  43. ncbi request reprint Pharmacological biotin supplementation maintains biotin status and function in rats administered dietary carbamazepine
    Sara C Rathman
    Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611, USA
    J Nutr 133:2857-62. 2003
    ..These results support the use of biotin supplementation as a concurrent strategy during CBZ administration to help maintain biotin status, function of biotin-dependent enzymes and decrease CBZ-induced lactate accumulation...
  44. ncbi request reprint Folate salvage in plants: pterin aldehyde reduction is mediated by multiple non-specific aldehyde reductases
    Alexandre Noiriel
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
    Plant J 51:378-89. 2007
    ..We conclude that pterin aldehyde salvage in plants involves multiple, generalist NADPH-linked reductases, and that the At1g10310 enzyme is typical of these and hence suitable for use in engineering studies of folate turnover...
  45. pmc Folate biofortification in tomatoes by engineering the pteridine branch of folate synthesis
    Rocio Diaz De La Garza
    Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 101:13720-5. 2004
    ..These results demonstrate that engineering a moderate increase in pteridine production can significantly enhance the folate content in food plants and that boosting the PABA supply can produce further gains...
  46. ncbi request reprint The analysis of folate and its metabolic precursors in biological samples
    Eoin P Quinlivan
    Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611, USA
    Anal Biochem 348:163-84. 2006
  47. ncbi request reprint Effect of food fortification on folic acid intake in the United States
    Eoin P Quinlivan
    Food Science and Human Nutrition Department, University of Florida, Gainesville, USA
    Am J Clin Nutr 77:221-5. 2003
    ....
  48. ncbi request reprint Reassessing folic acid consumption patterns in the United States (1999 2004): potential effect on neural tube defects and overexposure to folate
    Eoin P Quinlivan
    Biomedical Mass Spectrometry Laboratory, General Clinical Research Center, University of Florida, Gainesville, FL 32611 0370, USA
    Am J Clin Nutr 86:1773-9. 2007
    ..However, blood folate concentrations have decreased from their postfortification high as a result, in part, of decreasing food fortification concentrations and the popularity of low-carbohydrate weight-loss diets...
  49. ncbi request reprint Intestinal brush border membrane catalyzes hydrolysis of pyridoxine-5'-beta-D-glucoside and exhibits parallel developmental changes of hydrolytic activities toward pyridoxine-5'-beta-D-glucoside and lactose in rats
    Linda J Armada
    Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA
    J Nutr 132:2695-9. 2002
    ..84; P < 0.0001). These findings suggest that the hydrolysis of PNG is catalyzed at least partially at the brush border and that the bioavailability of PNG may be influenced by the residual LPH activity in children and adults...
  50. pmc Vitamin B-6 restriction tends to reduce the red blood cell glutathione synthesis rate without affecting red blood cell or plasma glutathione concentrations in healthy men and women
    Yvonne Lamers
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    Am J Clin Nutr 90:336-43. 2009
    ..Despite this obligatory role of PLP, previous studies from this laboratory showed that vitamin B-6 deficiency caused elevated glutathione concentrations in rat liver and human plasma...
  51. pmc FolX and FolM are essential for tetrahydromonapterin synthesis in Escherichia coli and Pseudomonas aeruginosa
    Anne Pribat
    University of Florida, Horticultural Sciences Department, Gainesville, FL 32611, USA
    J Bacteriol 192:475-82. 2010
    ....
  52. ncbi request reprint Capillary electrophoresis and high-performance liquid chromatography determination of polyglutamyl 5-methyltetrahydrofolate forms in citrus products
    Norman J Matella
    Food Science and Human Nutrition Department, University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, Florida 33850, USA
    J Agric Food Chem 53:2268-74. 2005
    ..e., 93 degrees C for 5 s, 88 degrees C for 15 s, and 82 degrees C for 30 s). Both methods were precise, reproducible, and potentially faster than traditional analytical procedures requiring enzymatic deconjugation and microbial assays...
  53. doi request reprint Developmental and feedforward control of the expression of folate biosynthesis genes in tomato fruit
    Jeffrey C Waller
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
    Mol Plant 3:66-77. 2010
    ..Microarray analysis showed that only 14 other transcripts (of 11 000 surveyed) increased in abundance by two-fold or more in high-folate fruit, demonstrating that the induction of folate pathway genes is relatively specific...
  54. ncbi request reprint Improved methods for the preparation of [3H]folate polyglutamates: biosynthesis with Lactobacillus casei and enzymatic synthesis with Escherichia coli folylpolyglutamate synthetase
    Valeria Naponelli
    Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611, USA
    Anal Biochem 371:127-34. 2007
    ..This enzymatic synthesis yielded predominantly tri-, tetra-, and pentaglutamyl species for the [(3)H]folate substrate...
  55. ncbi request reprint Plasma glutathione and cystathionine concentrations are elevated but cysteine flux is unchanged by dietary vitamin B-6 restriction in young men and women
    Steven R Davis
    Food Science andHuman Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    J Nutr 136:373-8. 2006
    ..These data indicate that glutathione homeostasis is altered by dietary vitamin B-6 deficiency and appears to be unrelated to cysteine flux under conditions of minimal amino acid intake as evaluated in this study...
  56. ncbi request reprint Activities of hepatic cytosolic and mitochondrial forms of serine hydroxymethyltransferase and hepatic glycine concentration are affected by vitamin B-6 intake in rats
    Jennifer B Scheer
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA
    J Nutr 135:233-8. 2005
    ..05), which suggests a functional effect of altered SHMT activity. Overall these results demonstrate the potential for disruption of SHMT-mediated one-carbon metabolism by inadequate vitamin B-6 intake...
  57. ncbi request reprint A mathematical model gives insights into nutritional and genetic aspects of folate-mediated one-carbon metabolism
    Michael C Reed
    Department of Mathematics, Duke University, Durham, NC 27708, USA
    J Nutr 136:2653-61. 2006
    ..These mathematical simulations of folate-mediated 1-carbon metabolism provide a cost-efficient approach to in silico experimentation that can complement and help guide laboratory studies...
  58. pmc In silico experimentation with a model of hepatic mitochondrial folate metabolism
    H Frederik Nijhout
    Department of Biology, Duke University, Durham, NC 27708, USA
    Theor Biol Med Model 3:40. 2006
    ....
  59. ncbi request reprint The impact of food fortification on folic acid intake in Canada
    Eoin P Quinlivan
    Can J Public Health 94:154. 2003
  60. ncbi request reprint Consumption of the folate breakdown product para-aminobenzoylglutamate contributes minimally to urinary folate catabolite excretion in humans: investigation using [(13)C(5)]para-aminobenzoylglutamate
    Marie A Caudill
    Human Nutrition and Food Science Department, Cal Poly Pomona University, Pomona, CA 91768, USA
    J Nutr 132:2613-6. 2002
    ..Overall, these results suggest that the contribution of dietary pABG to the urinary excretion of pABG and apABG is small...
  61. ncbi request reprint Homocysteine, B vitamins, and cardiovascular disease
    Eoin P Quinlivan
    N Engl J Med 355:206; author reply 209-11. 2006
  62. ncbi request reprint Folic acid and the prevention of neural-tube defects
    Eoin P Quinlivan
    N Engl J Med 350:2209-11; author reply 2209-11. 2004
  63. ncbi request reprint Differential kinetic behavior and distribution for pteroylglutamic acid and reduced folates: a revised hypothesis of the primary site of PteGlu metabolism in humans
    Anthony J A Wright
    Nutrition Department, Institute of Food Research, Norwich NR4 7UA, United Kingdom
    J Nutr 135:619-23. 2005
    ..Because of the observed differential plasma response and the hypothesized difference in the site of initial metabolism, the historical use of PteGlu as a "reference folate" in studies of folate bioavailability is seriously questioned...

Research Grants24

  1. NUTRITIONAL PROPERTIES OF PYRIDOXINE-BETA-GLUCOSIDE
    JESSE GREGORY; Fiscal Year: 2001
    ..These studies will provide new insight into the regulation of PNG hydrolase that is the rate-limiting enzyme for the utilization of glycosylated B6 in human diets. ..
  2. GENETIC EFFECTS-FOLATE-DEPENDENT ONE-CARBON METABOLISM
    JESSE GREGORY; Fiscal Year: 2004
    ..In total, these studies will yield new functional data regarding the effects of folate deficiency, and the influence of common polymorphism of MTHFR. ..
  3. Vitamin B6 Effects on One-Carbon Metabolism
    JESSE GREGORY; Fiscal Year: 2007
    ..These studies will expand our understanding of the functional impact of vitamin B6 inadequacy and will yield new information regarding nutritional dependence of human 1C metabolism. ..
  4. NUTRITIONAL PROPERTIES OF PYRIDOXINE-BETA-GLUCOSIDE
    JESSE GREGORY; Fiscal Year: 1993
    ....
  5. NUTRITIONAL PROPERTIES OF PYRIDOXINE-BETA-GLUCOSIDE
    JESSE GREGORY; Fiscal Year: 1990
    ....
  6. Vitamin B6 Effects on One-Carbon Metabolism
    Jesse F Gregory; Fiscal Year: 2010
    ..These studies will expand our understanding of the functional impact of vitamin B6 inadequacy in OC users and will provide a metabolically validated supplementation strategy to alleviate low B6 status and promote health in OC users. ..