Research Topics
Genomes and Genes
| Michael R GreenSummaryAffiliation: University of Massachusetts Medical School Country: USA Publications
Research Grants
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Detail Information
Publications
Eukaryotic transcription activation: right on targetMichael R Green
Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Mol Cell 18:399-402. 2005..Recently, the authentic targets of several yeast and mammalian activators have been determined, and the results of these studies have important implications for our understanding of transcriptional activation mechanisms...
Senescence: not just for tumor suppressionMichael R Green
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 134:562-4. 2008..In this issue, Krizhanovsky et al. (2008) report that senescence is an important player not only in tumor suppression but also in the response of liver tissue to injury...
Epigenetic silencing of the RASSF1A tumor suppressor gene through HOXB3-mediated induction of DNMT3B expressionRajendra Kumar Palakurthy
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Mol Cell 36:219-30. 2009..Analysis of human cancer cell lines indicates that the RASSF1A epigenetic silencing mechanism described here may be common in diverse cancer types...- Selective interaction between Trf3 and Taf3 required for early development and hematopoiesisDaniel O Hart
Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Dev Dyn 238:2540-9. 2009..Thus, a selective interaction between Trf3 and Taf3 is required for early zebrafish development and initiation of hematopoiesis. Finally, we provide evidence that TRF3 and TAF3 are also required for hematopoiesis initiation in the mouse... - A synthetic interaction screen identifies factors selectively required for proliferation and TERT transcription in p53-deficient human cancer cellsLi Xie
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
PLoS Genet 8:e1003151. 2012..Our collective results identify a regulatory pathway involving ETV1, ATR, and TERT that is preferentially important for proliferation of diverse p53- cancer cells... - A pathway of sequential arginine-serine-rich domain-splicing signal interactions during mammalian spliceosome assemblyHaihong Shen
Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Cell 16:363-73. 2004..We propose that direct contact with splicing signals is a general mechanism by which RS domains promote splicing... - A genome-wide shRNA screen identifies GAS1 as a novel melanoma metastasis suppressor geneStephane Gobeil
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 22:2932-40. 2008..Thus, we developed a genome-wide shRNA screening strategy that enables the discovery of new metastasis suppressor genes... - Efficacy of IGFBP7 for treatment of metastatic melanoma and other cancers in mouse models and human cell linesNarendra Wajapeyee
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Cancer Ther 8:3009-14. 2009..The results presented here, in conjunction with those from previous studies, justify the further development of IGFBP7 as an anticancer agent... - Oncogenic BRAF induces senescence and apoptosis through pathways mediated by the secreted protein IGFBP7Narendra Wajapeyee
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 132:363-74. 2008..Immunohistochemical analysis of human skin, nevi, and melanoma samples implicates loss of IGFBP7 expression as a critical step in melanoma genesis... - GABP transcription factor is required for development of chronic myelogenous leukemia via its control of PRKD2Zhong fa Yang
Division of Hematology Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA
Proc Natl Acad Sci U S A 110:2312-7. 2013..Thus, GABP is required for HSC cell cycle entry and CML development through its control of PRKD2. This offers a potential therapeutic target in leukemia... - RS domains contact splicing signals and promote splicing by a common mechanism in yeast through humansHaihong Shen
Howard Hughes Medical Institute and Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 20:1755-65. 2006..Our results reveal a common mechanism of RS domain function in yeast through humans... - Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4Ling Lin
Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 109:1997-2002. 2012..Finally, we show that although Tra1 is targeted by other activators, these interactions are unaffected by GID mutations, revealing an unanticipated specificity of the Gal4-Tra1 interaction... - RS domain-splicing signal interactions in splicing of U12-type and U2-type intronsHaihong Shen
Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nat Struct Mol Biol 14:597-603. 2007..Our results reveal alternative interactions between the RS domain and 5' splice site region that coincide with remodeling of the spliceosome between the two catalytic steps... - BCR-ABL suppresses autophagy through ATF5-mediated regulation of mTOR transcriptionZhi Sheng
Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
Blood 118:2840-8. 2011..We demonstrate that imatinib-induced autophagy is because of inhibition of the BCR-ABL/PI3K/AKT/FOXO4/ATF5/mTOR pathway that we have identified in this study... - F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA damageManas K Santra
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nature 459:722-5. 2009..Our results reveal FBXO31 as a regulator of the G1/S transition that is specifically required for DNA damage-induced growth arrest... - Senescence induction in human fibroblasts and hematopoietic progenitors by leukemogenic fusion proteinsNarendra Wajapeyee
Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA
Blood 115:5057-60. 2010..Our results reveal diverse pathways for oncogene-induced senescence and further suggest that leukemias harbor genetic or epigenetic alterations that inactivate senescence induction genes... - A genome-wide RNA interference screen reveals an essential CREB3L2-ATF5-MCL1 survival pathway in malignant glioma with therapeutic implicationsZhi Sheng
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
Nat Med 16:671-7. 2010..Our results demonstrate that ATF5 is essential in malignant glioma genesis and reveal that the ATF5-mediated survival pathway described here provides potential therapeutic targets for treatment of malignant glioma... - ChIPpeakAnno: a Bioconductor package to annotate ChIP-seq and ChIP-chip dataLihua J Zhu
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
BMC Bioinformatics 11:237. 2010..However, summarizing these tracks can be a daunting task, particularly if there are a large number of binding sites or the binding sites are distributed widely across the genome... - Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs)Stephan P Persengiev
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
RNA 10:12-8. 2004..The potential for this widespread, nonspecific effect on mammalian gene expression must be carefully considered in the design of siRNA experiments and therapeutic applications... - Identification of a protein, G0S2, that lacks Bcl-2 homology domains and interacts with and antagonizes Bcl-2Christian Welch
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Cancer Res 69:6782-9. 2009..Our results reveal a novel proapoptotic factor that is induced by TNF-alpha through NF-kappaB and that interacts with and antagonizes Bcl-2... - HIV-1 Tat stimulates transcription complex assembly through recruitment of TBP in the absence of TAFsTamal Raha
Howard Hughes Medical Institute, Program in Gene Function, University of Massachusetts Medical School, Worcester, USA
PLoS Biol 3:e44. 2005..Thus, in mammalian cells transcription of protein-coding genes involves alternative TCs that differ by the presence or absence of TAFs... - In vivo target of a transcriptional activator revealed by fluorescence resonance energy transferSukesh R Bhaumik
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 18:333-43. 2004..The FRET assay we describe is a general method that can be used to identify the in vivo targets of other activators... - Characterization of enhancer function from genome-wide analysesGlenn A Maston
Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Annu Rev Genomics Hum Genet 13:29-57. 2012..Going forward, the integration of multiple genome-wide data sets should become a standard approach to elucidate higher-order regulatory interactions... - Apoptin nucleocytoplasmic shuttling is required for cell type-specific localization, apoptosis, and recruitment of the anaphase-promoting complex/cyclosome to PML bodiesDestin W Heilman
Howard Hughes Medical Institute, and Program in Gene Function and Expression, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
J Virol 80:7535-45. 2006..Apoptin expression in transformed cells induces the formation of PML nuclear bodies and recruits APC/C to these subnuclear structures. Our results reveal a mechanism for the selective killing of transformed cells by Apoptin... - Identification of direct DAF-16 targets controlling longevity, metabolism and diapause by chromatin immunoprecipitationSeung Wook Oh
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nat Genet 38:251-7. 2006..We also demonstrate that DAF-16 is recruited to multiple promoters to coordinate regulation of its downstream targets. The large number of target genes discovered provides insight into how DAF-16 controls diverse biological functions... - The anaphase promoting complex: a critical target for viral proteins and anti-cancer drugsDestin W Heilman
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Cell Cycle 4:560-3. 2005.... - When enough is enough: genetic diseases associated with transcriptional derepressionDavide Gabellini
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Curr Opin Genet Dev 14:301-7. 2004.... - An elaborate pathway required for Ras-mediated epigenetic silencingClaude Gazin
Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
Nature 449:1073-7. 2007..Our results show that Ras-mediated epigenetic silencing occurs through a specific, complex, pathway involving components that are required for maintenance of a fully transformed phenotype... - Transcription factor ATF5 is required for terminal differentiation and survival of olfactory sensory neuronsShu Zong Wang
Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 109:18589-94. 2012..Thus, ATF5 is required for terminal differentiation and survival of OSNs... - TRF3, a TATA-box-binding protein-related factor, is vertebrate-specific and widely expressedStephan P Persengiev
Howard Hughes Medical Institute and Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 100:14887-91. 2003..In summary, TRF3 is a highly conserved vertebrate-specific TRF whose phylogenetic conservation, expression pattern, and other properties are distinct from those of TBP and all other TRFs... - A single polypyrimidine tract binding protein (PTB) binding site mediates splicing inhibition at mouse IgM exons M1 and M2Haihong Shen
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
RNA 10:787-94. 2004..Collectively, our results indicate that a single PTB binding site can function as an inhibitor that regulates alternative splicing both in vitro and in vivo... - Targeting a TAF to make muscleDaniel O Hart
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Cell 32:164-6. 2008..2008) elucidate the basis by which the muscle-specific activator MyoD recruits the core transcription machinery to the promoter of a key regulatory gene involved in myogenic differentiation... - p53-mediated inhibition of angiogenesis through up-regulation of a collagen prolyl hydroxylaseJose G Teodoro
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Science 313:968-71. 2006..Our results reveal a genetic and biochemical linkage between the p53 tumor suppressor pathway and the synthesis of antiangiogenic collagen fragments... - A cell-surface receptor for lipocalin 24p3 selectively mediates apoptosis and iron uptakeLaxminarayana R Devireddy
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 123:1293-305. 2005..Our results reveal an unanticipated role for intracellular iron regulation in an apoptotic pathway relevant to BCR-ABL-induced myeloproliferative disease and its treatment... - The viral protein Apoptin associates with the anaphase-promoting complex to induce G2/M arrest and apoptosis in the absence of p53Jose G Teodoro
Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester 01605, USA
Genes Dev 18:1952-7. 2004..Our results explain the ability of Apoptin to induce apoptosis in the absence of p53 and suggest that the APC/C is an attractive target for anticancer drug development... - An activating transcription factor 5-mediated survival pathway as a target for cancer therapy?Zhi Sheng
Howard Hughes Medical Institute, Program in Gene Function, University of Massachusetts Medical School, Worcester, MA 01605, USA
Oncotarget 1:457-60. 2010..In this perspective, we summarize recent advances in ATF5 research, focusing on its role in promoting cancer and its potential as a target for cancer therapy... - Maternal Rnf12/RLIM is required for imprinted X-chromosome inactivation in miceJongdae Shin
Program in Gene Function and Expression, University of Massachusetts Medical School UMMS, Worcester, Massachusetts 01605, USA
Nature 467:977-81. 2010..These results assign crucial functions to the maternal deposit of Rnf12/RLIM for the initiation of imprinted XCI... - Non-canonical TAF complexes regulate active promoters in human embryonic stem cellsGlenn A Maston
Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, United States Howard Hughes Medical Institute, Chevy Chase, United States
elife 1:e00068. 2012..Thus, the selective expression and use of TAFs underlies the ability of hESCs to self-renew.DOI:http://dx.doi.org/10.7554/eLife.00068.001... - Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assemblyWu Cheng Shen
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
EMBO J 22:3395-402. 2003..Collectively, our results confirm and extend the proposal that individual TAFs have selective transcriptional roles and distinct functions... - Arginine-serine-rich domains bound at splicing enhancers contact the branchpoint to promote prespliceosome assemblyHaihong Shen
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605 USA
Mol Cell 13:367-76. 2004..We conclude that the ESE-bound RS domain functions by contacting the branchpoint to promote prespliceosome assembly... - Initiation of zebrafish haematopoiesis by the TATA-box-binding protein-related factor Trf3Daniel O Hart
Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
Nature 450:1082-5. 2007..Thus, in zebrafish, commitment of mesoderm to the haematopoietic lineage occurs through a transcription factor pathway initiated by a TBP-related factor... - Irf3 polymorphism alters induction of interferon beta in response to Listeria monocytogenes infectionOleg Garifulin
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts, USA
PLoS Genet 3:1587-97. 2007..monocytogenes infection. Thus, the C57BL/6ByJ mouse strain serves as an example of how a mammalian host can counter bacterial virulence strategies by introducing subtle alteration of noncoding sequences... - Transcriptional regulatory elements in the human genomeGlenn A Maston
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Annu Rev Genomics Hum Genet 7:29-59. 2006..Finally, we discuss the methods currently used to identify transcriptional regulatory elements, and the ability of these methods to be scaled up for the purpose of annotating the entire human genome... - Transcription and signalling pathways involved in BCR-ABL-mediated misregulation of 24p3 and 24p3RZhi Sheng
Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
EMBO J 28:866-76. 2009..Our results reveal diverse signalling/transcription pathways that regulate 24p3 and 24p3R expression in response to BCR-ABL and are directly relevant to the treatment of BCR-ABL(+) disease... - The Blk pathway functions as a tumor suppressor in chronic myeloid leukemia stem cellsHaojian Zhang
Department of Medicine, Division of Hematology Oncology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
Nat Genet 44:861-71. 2012..Blk also suppresses the proliferation of human CML stem cells. Our results show the feasibility of selectively targeting LSCs, an approach that should be applicable to other cancers... - Facioscapulohumeral muscular dystrophy in mice overexpressing FRG1Davide Gabellini
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nature 439:973-7. 2006..Collectively, our results suggest that FSHD results from inappropriate overexpression of FRG1 in skeletal muscle, which leads to abnormal alternative splicing of specific pre-mRNAs... - Inappropriate gene activation in FSHD: a repressor complex binds a chromosomal repeat deleted in dystrophic muscleDavide Gabellini
Howard Hughes Medical Institute, Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 110:339-48. 2002..Based upon these results, we propose that deletion of D4Z4 leads to the inappropriate transcriptional derepression of 4q35 genes resulting in disease... - Differential requirement of SAGA components for recruitment of TATA-box-binding protein to promoters in vivoSukesh R Bhaumik
Howard Hughes Medical Institute, Programs in Gene Expression and Function and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Mol Cell Biol 22:7365-71. 2002..In summary, we show that SAGA-dependent promoters require different combinations of SAGA components for TBP recruitment, revealing a complex combinatorial network for transcription activation in vivo... - Transcriptional program of apoptosis induction following interleukin 2 deprivation: identification of RC3, a calcium/calmodulin binding protein, as a novel proapoptotic factorLaxminarayana R Devireddy
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Mol Cell Biol 23:4532-41. 2003..Based upon these results, we propose that IL-2 deprivation raises the level of RC3 and other apoptotic factors, which induce apoptosis by increasing the intracellular Ca(2+) concentration... - Transcriptional derepression as a cause of genetic diseasesDavide Gabellini
Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Curr Opin Genet Dev 13:239-45. 2003..The putative target genes, whose inappropriate expression is thought to cause disease, have remained elusive but are being searched for intensively... - Inhibition of apoptosis by ATFx: a novel role for a member of the ATF/CREB family of mammalian bZIP transcription factorsStephan P Persengiev
Howard Hughes Medical Institute and Programs in Molecular Medicine and Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 16:1806-14. 2002..However, constitutive expression of ATFx renders cells resistant to 24p3-mediated apoptosis. Collectively, our results indicate that ATFx is an anti-apoptotic factor, a novel role for an ATF protein... - Interaction of Gal4p with components of transcription machinery in vivoSukesh R Bhaumik
Department of Biochemistry and Molecular Biology, Southern Illinois University, Carbondale, Illinois 62901, USA
Methods Enzymol 370:445-54. 2003 - Cell motility is controlled by SF2/ASF through alternative splicing of the Ron protooncogeneClaudia Ghigna
Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, Pavia, Italy
Mol Cell 20:881-90. 2005..This demonstrates a direct link between SF2/ASF-regulated splicing and cell motility, an activity important for embryogenesis, tissue formation, and tumor metastasis...
Research Grants
- SPLICING OF MRNA PRECURSORSMichael R Green; Fiscal Year: 2010....
- MECHANISMS OF EUKARYOTIC TRANSCRITIONAL ACTIVATIONMichael Green; Fiscal Year: 2003..Such a method would be extremely useful in many areas of biology and medicine. ..
- SPLICING OF MRNA PRECURSORSMichael Green; Fiscal Year: 2000..We have identified the human protein that contracts the 3prime splice site and propose to isolate a molecular clone and use this to study second-step mechanisms. ..
- HIV-1 REV PROTEIN--BIOCHEMICAL MECHANISMSMichael Green; Fiscal Year: 2000..These studies will be extended and the development of more potent and specific Rev inhibitors is proposed. ..
- TRANSCRIPTION ACTIVATION BY HIV, TAT, HTLV TAX & HBV PXMichael Green; Fiscal Year: 2004..However, BEF works by a different mechanism, as a molecular chaperone. Experiments are proposed to further study the mechanism of BEF action. ..
- SPLICING OF MRNA PRECURSORSMichael Green; Fiscal Year: 2004..They function by providing binding site for the SR family of proteins that contain arginine-serine rich (RS) domains. Experiments are proposed to study how RS domains promote spliceosome assembly and splicing. ..
- MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL REGULATIONMichael R Green; Fiscal Year: 2010..A specific objective of the application is to study the mechanism by which tumor suppressor genes become transcriptionally silenced during cancer development. ..
- Role of Lipocalin 24p3 in Apoptosis and LeukemiaMichael R Green; Fiscal Year: 2010..We have derived 24p3 homozygous knockout mice, which will be used to study the contribution of the 24p3/24p3R proapoptotic pathway to BCR/ABL-induced myeloproliferative disease. ..
- Role of Lipocalin 24p3 in Apoptosis and LeukemiaMichael Green; Fiscal Year: 2009..We have derived 24p3 homozygous knockout mice, which will be used to study the contribution of the 24p3/24p3R proapoptotic pathway to BCR/ABL-induced myeloproliferative disease. ..
- SPLICING OF MRNA PRECURSORSMichael Green; Fiscal Year: 2007..We will use this experimental approach to study the mechanism of action of other splicing factors and regulators. ..
- Role of Lipocalin 24p3 in Apoptosis and LeukemiaMichael Green; Fiscal Year: 2007..We have derived 24p3 homozygous knockout mice, which will be used to study the contribution of the 24p3/24p3R proapoptotic pathway to BCR/ABL-induced myeloproliferative disease. ..
- Mechanisms of Eukaryotic Transcriptional ActivationMichael Green; Fiscal Year: 2007..Using diverse experimental systems (mammalian tissue-culture cells, zebrafish, mice), we will identify TRF3 target genes, clone TRF3 interacting proteins, and determine the basis of selective TRF3 function. ..
- Intracellular Localization of HIV-1 RNAs by Rev&MatrixMichael Green; Fiscal Year: 2005..Finally, our results suggest a novel method for inhibiting the activity of any mRNA. This "mRNA nuclear entrapment" strategy will be developed and tested using HIV-1 replication as a model system. ..
- TRANSCRIPTIONAL ACTIVATION BY AD E1A AND HSV VP16Michael Green; Fiscal Year: 1999..Experiments are proposed to understand in greater detail regulation of ATF-2 transcriptional activity. ..
- SPLICING OF MRNA PRECURSORSMichael Green; Fiscal Year: 1992..Finally, experiments are proposed to determine how splice sites are accurately selected and the mechanisms involved in regulated alternative splicing...
- TRANSCRIPTION ACTIVATION BY HIV TAT, HTLV TAX AND HBV PXMichael Green; Fiscal Year: 1999..We will isolate a cDNA clone for BEF and use this to study its role in viral gene expression and transformation. ..
- SPLICING OF MRNA PRECURSORSMichael Green; Fiscal Year: 1993..We have made a series of observations suggesting the potential involvement of a glycolytic enzyme, GAPDH, in RNA export. Experiments are proposed to investigate this possibility...
- TRANSCRIPTIONAL ACTIVATION BY THE ADENOVIRUS ELA PROTEINMichael Green; Fiscal Year: 1993..In summary, we propose a broad and comprehensive set of experiments the results of which should enrich our knowledge about the adenovirus E1a protein and the cellular proteins through which E1a acts...
- ADENOVIRUS TRANSCRIPTIONAL REGULATION & TRANSFORMATIONMichael Green; Fiscal Year: 1991..Ultimately, we hope to develop in vitro systems that faithfully carry out these Ela-mediated transcriptional regulatory function thus allowing the biochemical mechanisms involved in Ela transcription regulation to be addressed...
- TRANSCRIPTION ACTIVATION BY THE HIV TAT AND HTLV TAXMichael Green; Fiscal Year: 1993
- HIV-1 REV PROTEIN--BIOCHEMICAL MECHANISMSMichael Green; Fiscal Year: 1993..These dominant-negative Rev mutants have the potential to block viral replication. The applicant intends to explore the basis of the dominant-negative phenotype and to test their ability to function as anti-viral agents...