Michael R Green

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. doi request reprint Senescence: not just for tumor suppression
    Michael R Green
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 134:562-4. 2008
  2. ncbi request reprint Eukaryotic transcription activation: right on target
    Michael R Green
    Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell 18:399-402. 2005
  3. pmc Epigenetic silencing of the RASSF1A tumor suppressor gene through HOXB3-mediated induction of DNMT3B expression
    Rajendra Kumar Palakurthy
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Mol Cell 36:219-30. 2009
  4. pmc Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis
    Daniel O Hart
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Dev Dyn 238:2540-9. 2009
  5. pmc GABP transcription factor is required for development of chronic myelogenous leukemia via its control of PRKD2
    Zhong fa Yang
    Division of Hematology Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 110:2312-7. 2013
  6. pmc A synthetic interaction screen identifies factors selectively required for proliferation and TERT transcription in p53-deficient human cancer cells
    Li Xie
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Genet 8:e1003151. 2012
  7. pmc Oncogenic BRAF induces senescence and apoptosis through pathways mediated by the secreted protein IGFBP7
    Narendra Wajapeyee
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 132:363-74. 2008
  8. pmc Efficacy of IGFBP7 for treatment of metastatic melanoma and other cancers in mouse models and human cell lines
    Narendra Wajapeyee
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cancer Ther 8:3009-14. 2009
  9. ncbi request reprint A pathway of sequential arginine-serine-rich domain-splicing signal interactions during mammalian spliceosome assembly
    Haihong Shen
    Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 16:363-73. 2004
  10. pmc A genome-wide shRNA screen identifies GAS1 as a novel melanoma metastasis suppressor gene
    Stephane Gobeil
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 22:2932-40. 2008

Research Grants

Collaborators

Detail Information

Publications64

  1. doi request reprint Senescence: not just for tumor suppression
    Michael R Green
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 134:562-4. 2008
    ..In this issue, Krizhanovsky et al. (2008) report that senescence is an important player not only in tumor suppression but also in the response of liver tissue to injury...
  2. ncbi request reprint Eukaryotic transcription activation: right on target
    Michael R Green
    Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell 18:399-402. 2005
    ..Recently, the authentic targets of several yeast and mammalian activators have been determined, and the results of these studies have important implications for our understanding of transcriptional activation mechanisms...
  3. pmc Epigenetic silencing of the RASSF1A tumor suppressor gene through HOXB3-mediated induction of DNMT3B expression
    Rajendra Kumar Palakurthy
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Mol Cell 36:219-30. 2009
    ..Analysis of human cancer cell lines indicates that the RASSF1A epigenetic silencing mechanism described here may be common in diverse cancer types...
  4. pmc Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis
    Daniel O Hart
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Dev Dyn 238:2540-9. 2009
    ..Thus, a selective interaction between Trf3 and Taf3 is required for early zebrafish development and initiation of hematopoiesis. Finally, we provide evidence that TRF3 and TAF3 are also required for hematopoiesis initiation in the mouse...
  5. pmc GABP transcription factor is required for development of chronic myelogenous leukemia via its control of PRKD2
    Zhong fa Yang
    Division of Hematology Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 110:2312-7. 2013
    ..Thus, GABP is required for HSC cell cycle entry and CML development through its control of PRKD2. This offers a potential therapeutic target in leukemia...
  6. pmc A synthetic interaction screen identifies factors selectively required for proliferation and TERT transcription in p53-deficient human cancer cells
    Li Xie
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Genet 8:e1003151. 2012
    ..Our collective results identify a regulatory pathway involving ETV1, ATR, and TERT that is preferentially important for proliferation of diverse p53- cancer cells...
  7. pmc Oncogenic BRAF induces senescence and apoptosis through pathways mediated by the secreted protein IGFBP7
    Narendra Wajapeyee
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 132:363-74. 2008
    ..Immunohistochemical analysis of human skin, nevi, and melanoma samples implicates loss of IGFBP7 expression as a critical step in melanoma genesis...
  8. pmc Efficacy of IGFBP7 for treatment of metastatic melanoma and other cancers in mouse models and human cell lines
    Narendra Wajapeyee
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cancer Ther 8:3009-14. 2009
    ..The results presented here, in conjunction with those from previous studies, justify the further development of IGFBP7 as an anticancer agent...
  9. ncbi request reprint A pathway of sequential arginine-serine-rich domain-splicing signal interactions during mammalian spliceosome assembly
    Haihong Shen
    Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 16:363-73. 2004
    ..We propose that direct contact with splicing signals is a general mechanism by which RS domains promote splicing...
  10. pmc A genome-wide shRNA screen identifies GAS1 as a novel melanoma metastasis suppressor gene
    Stephane Gobeil
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 22:2932-40. 2008
    ..Thus, we developed a genome-wide shRNA screening strategy that enables the discovery of new metastasis suppressor genes...
  11. pmc RS domains contact splicing signals and promote splicing by a common mechanism in yeast through humans
    Haihong Shen
    Howard Hughes Medical Institute and Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 20:1755-65. 2006
    ..Our results reveal a common mechanism of RS domain function in yeast through humans...
  12. pmc BCR-ABL suppresses autophagy through ATF5-mediated regulation of mTOR transcription
    Zhi Sheng
    Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Blood 118:2840-8. 2011
    ..We demonstrate that imatinib-induced autophagy is because of inhibition of the BCR-ABL/PI3K/AKT/FOXO4/ATF5/mTOR pathway that we have identified in this study...
  13. pmc MEN1 is a melanoma tumor suppressor that preserves genomic integrity by stimulating transcription of genes that promote homologous recombination-directed DNA repair
    Minggang Fang
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Mol Cell Biol 33:2635-47. 2013
    ..Collectively, our results indicate that MEN1 is a melanoma tumor suppressor that functions by stimulating the transcription of genes involved in HR-directed DNA repair...
  14. pmc Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4
    Ling Lin
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 109:1997-2002. 2012
    ..Finally, we show that although Tra1 is targeted by other activators, these interactions are unaffected by GID mutations, revealing an unanticipated specificity of the Gal4-Tra1 interaction...
  15. ncbi request reprint RS domain-splicing signal interactions in splicing of U12-type and U2-type introns
    Haihong Shen
    Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Struct Mol Biol 14:597-603. 2007
    ..Our results reveal alternative interactions between the RS domain and 5' splice site region that coincide with remodeling of the spliceosome between the two catalytic steps...
  16. pmc F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA damage
    Manas K Santra
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 459:722-5. 2009
    ..Our results reveal FBXO31 as a regulator of the G1/S transition that is specifically required for DNA damage-induced growth arrest...
  17. pmc Senescence induction in human fibroblasts and hematopoietic progenitors by leukemogenic fusion proteins
    Narendra Wajapeyee
    Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA
    Blood 115:5057-60. 2010
    ..Our results reveal diverse pathways for oncogene-induced senescence and further suggest that leukemias harbor genetic or epigenetic alterations that inactivate senescence induction genes...
  18. pmc A genome-wide RNA interference screen reveals an essential CREB3L2-ATF5-MCL1 survival pathway in malignant glioma with therapeutic implications
    Zhi Sheng
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Nat Med 16:671-7. 2010
    ..Our results demonstrate that ATF5 is essential in malignant glioma genesis and reveal that the ATF5-mediated survival pathway described here provides potential therapeutic targets for treatment of malignant glioma...
  19. pmc Identification of a protein, G0S2, that lacks Bcl-2 homology domains and interacts with and antagonizes Bcl-2
    Christian Welch
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Cancer Res 69:6782-9. 2009
    ..Our results reveal a novel proapoptotic factor that is induced by TNF-alpha through NF-kappaB and that interacts with and antagonizes Bcl-2...
  20. pmc TRIM37 is a new histone H2A ubiquitin ligase and breast cancer oncoprotein
    Sanchita Bhatnagar
    1 Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA 2 Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 516:116-20. 2014
    ..Collectively, our results reveal TRIM37 as an oncogenic H2A ubiquitin ligase that is overexpressed in a subset of breast cancers and promotes transformation by facilitating silencing of tumour suppressors and other genes...
  21. pmc ChIPpeakAnno: a Bioconductor package to annotate ChIP-seq and ChIP-chip data
    Lihua J Zhu
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    BMC Bioinformatics 11:237. 2010
    ..However, summarizing these tracks can be a daunting task, particularly if there are a large number of binding sites or the binding sites are distributed widely across the genome...
  22. pmc Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs)
    Stephan P Persengiev
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    RNA 10:12-8. 2004
    ..The potential for this widespread, nonspecific effect on mammalian gene expression must be carefully considered in the design of siRNA experiments and therapeutic applications...
  23. pmc Resistance to vemurafenib resulting from a novel mutation in the BRAFV600E kinase domain
    Timothy R Wagenaar
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
    Pigment Cell Melanoma Res 27:124-33. 2014
    ..Our results identify a novel vemurafenib-resistant mutant and provide insights into the treatment for melanomas bearing this mutation. ..
  24. pmc U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs
    Jermaine L Jenkins
    Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Nucleic Acids Res 41:3859-73. 2013
    ..Our results highlight both local and global conformational selection as a means for universal 3' splice site recognition by U2AF(65)...
  25. pmc HIV-1 Tat stimulates transcription complex assembly through recruitment of TBP in the absence of TAFs
    Tamal Raha
    Howard Hughes Medical Institute, Program in Gene Function, University of Massachusetts Medical School, Worcester, USA
    PLoS Biol 3:e44. 2005
    ..Thus, in mammalian cells transcription of protein-coding genes involves alternative TCs that differ by the presence or absence of TAFs...
  26. pmc In vivo target of a transcriptional activator revealed by fluorescence resonance energy transfer
    Sukesh R Bhaumik
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 18:333-43. 2004
    ..The FRET assay we describe is a general method that can be used to identify the in vivo targets of other activators...
  27. pmc An elaborate pathway required for Ras-mediated epigenetic silencing
    Claude Gazin
    Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 449:1073-7. 2007
    ..Our results show that Ras-mediated epigenetic silencing occurs through a specific, complex, pathway involving components that are required for maintenance of a fully transformed phenotype...
  28. pmc A large-scale RNAi-based mouse tumorigenesis screen identifies new lung cancer tumor suppressors that repress FGFR signaling
    Ling Lin
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts
    Cancer Discov 4:1168-81. 2014
    ..Our results indicate that increased FGFR signaling promotes tumorigenesis in many hLSCCs that lack FGFR1 amplification or activating mutations...
  29. ncbi request reprint The anaphase promoting complex: a critical target for viral proteins and anti-cancer drugs
    Destin W Heilman
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Cell Cycle 4:560-3. 2005
    ....
  30. doi request reprint Characterization of enhancer function from genome-wide analyses
    Glenn A Maston
    Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Annu Rev Genomics Hum Genet 13:29-57. 2012
    ..Going forward, the integration of multiple genome-wide data sets should become a standard approach to elucidate higher-order regulatory interactions...
  31. pmc Apoptin nucleocytoplasmic shuttling is required for cell type-specific localization, apoptosis, and recruitment of the anaphase-promoting complex/cyclosome to PML bodies
    Destin W Heilman
    Howard Hughes Medical Institute, and Program in Gene Function and Expression, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    J Virol 80:7535-45. 2006
    ..Apoptin expression in transformed cells induces the formation of PML nuclear bodies and recruits APC/C to these subnuclear structures. Our results reveal a mechanism for the selective killing of transformed cells by Apoptin...
  32. ncbi request reprint When enough is enough: genetic diseases associated with transcriptional derepression
    Davide Gabellini
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Curr Opin Genet Dev 14:301-7. 2004
    ....
  33. ncbi request reprint Identification of direct DAF-16 targets controlling longevity, metabolism and diapause by chromatin immunoprecipitation
    Seung Wook Oh
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Genet 38:251-7. 2006
    ..We also demonstrate that DAF-16 is recruited to multiple promoters to coordinate regulation of its downstream targets. The large number of target genes discovered provides insight into how DAF-16 controls diverse biological functions...
  34. pmc The BRAF oncoprotein functions through the transcriptional repressor MAFG to mediate the CpG Island Methylator phenotype
    Minggang Fang
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 55:904-15. 2014
    ..Analysis of transcriptionally silenced CIMP genes in KRAS-positive CRCs indicates that different oncoproteins direct the assembly of distinct repressor complexes on common promoters. ..
  35. ncbi request reprint p53-mediated inhibition of angiogenesis through up-regulation of a collagen prolyl hydroxylase
    Jose G Teodoro
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Science 313:968-71. 2006
    ..Our results reveal a genetic and biochemical linkage between the p53 tumor suppressor pathway and the synthesis of antiangiogenic collagen fragments...
  36. pmc An activating transcription factor 5-mediated survival pathway as a target for cancer therapy?
    Zhi Sheng
    Howard Hughes Medical Institute, Program in Gene Function, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Oncotarget 1:457-60. 2010
    ..In this perspective, we summarize recent advances in ATF5 research, focusing on its role in promoting cancer and its potential as a target for cancer therapy...
  37. pmc A therapeutically targetable mechanism of BCR-ABL-independent imatinib resistance in chronic myeloid leukemia
    Leyuan Ma
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Sci Transl Med 6:252ra121. 2014
    ..Collectively, our results identify a therapeutically targetable mechanism of BCR-ABL-independent IM resistance in CML and CML stem cells. ..
  38. pmc A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype
    Ryan W Serra
    Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, United States
    elife 3:e02313. 2014
    ..Finally, we show that ZNF304 also directs transcriptional silencing of INK4-ARF in human embryonic stem cells. DOI: http://dx.doi.org/10.7554/eLife.02313.001. ..
  39. pmc TRF3, a TATA-box-binding protein-related factor, is vertebrate-specific and widely expressed
    Stephan P Persengiev
    Howard Hughes Medical Institute and Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 100:14887-91. 2003
    ..In summary, TRF3 is a highly conserved vertebrate-specific TRF whose phylogenetic conservation, expression pattern, and other properties are distinct from those of TBP and all other TRFs...
  40. pmc Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly
    Wu Cheng Shen
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    EMBO J 22:3395-402. 2003
    ..Collectively, our results confirm and extend the proposal that individual TAFs have selective transcriptional roles and distinct functions...
  41. pmc Transcription factor ATF5 is required for terminal differentiation and survival of olfactory sensory neurons
    Shu Zong Wang
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 109:18589-94. 2012
    ..Thus, ATF5 is required for terminal differentiation and survival of OSNs...
  42. pmc A single polypyrimidine tract binding protein (PTB) binding site mediates splicing inhibition at mouse IgM exons M1 and M2
    Haihong Shen
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    RNA 10:787-94. 2004
    ..Collectively, our results indicate that a single PTB binding site can function as an inhibitor that regulates alternative splicing both in vitro and in vivo...
  43. pmc Maternal Rnf12/RLIM is required for imprinted X-chromosome inactivation in mice
    Jongdae Shin
    Program in Gene Function and Expression, University of Massachusetts Medical School UMMS, Worcester, Massachusetts 01605, USA
    Nature 467:977-81. 2010
    ..These results assign crucial functions to the maternal deposit of Rnf12/RLIM for the initiation of imprinted XCI...
  44. pmc The viral protein Apoptin associates with the anaphase-promoting complex to induce G2/M arrest and apoptosis in the absence of p53
    Jose G Teodoro
    Howard Hughes Medical Institute, Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester 01605, USA
    Genes Dev 18:1952-7. 2004
    ..Our results explain the ability of Apoptin to induce apoptosis in the absence of p53 and suggest that the APC/C is an attractive target for anticancer drug development...
  45. doi request reprint Targeting a TAF to make muscle
    Daniel O Hart
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 32:164-6. 2008
    ..2008) elucidate the basis by which the muscle-specific activator MyoD recruits the core transcription machinery to the promoter of a key regulatory gene involved in myogenic differentiation...
  46. ncbi request reprint A cell-surface receptor for lipocalin 24p3 selectively mediates apoptosis and iron uptake
    Laxminarayana R Devireddy
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 123:1293-305. 2005
    ..Our results reveal an unanticipated role for intracellular iron regulation in an apoptotic pathway relevant to BCR-ABL-induced myeloproliferative disease and its treatment...
  47. ncbi request reprint Transcriptional regulatory elements in the human genome
    Glenn A Maston
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Annu Rev Genomics Hum Genet 7:29-59. 2006
    ..Finally, we discuss the methods currently used to identify transcriptional regulatory elements, and the ability of these methods to be scaled up for the purpose of annotating the entire human genome...
  48. pmc Transcription and signalling pathways involved in BCR-ABL-mediated misregulation of 24p3 and 24p3R
    Zhi Sheng
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    EMBO J 28:866-76. 2009
    ..Our results reveal diverse signalling/transcription pathways that regulate 24p3 and 24p3R expression in response to BCR-ABL and are directly relevant to the treatment of BCR-ABL(+) disease...
  49. pmc Non-canonical TAF complexes regulate active promoters in human embryonic stem cells
    Glenn A Maston
    Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, United States Howard Hughes Medical Institute, Chevy Chase, United States
    elife 1:e00068. 2012
    ..Thus, the selective expression and use of TAFs underlies the ability of hESCs to self-renew.DOI:http://dx.doi.org/10.7554/eLife.00068.001...
  50. pmc Initiation of zebrafish haematopoiesis by the TATA-box-binding protein-related factor Trf3
    Daniel O Hart
    Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 450:1082-5. 2007
    ..Thus, in zebrafish, commitment of mesoderm to the haematopoietic lineage occurs through a transcription factor pathway initiated by a TBP-related factor...
  51. ncbi request reprint Arginine-serine-rich domains bound at splicing enhancers contact the branchpoint to promote prespliceosome assembly
    Haihong Shen
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605 USA
    Mol Cell 13:367-76. 2004
    ..We conclude that the ESE-bound RS domain functions by contacting the branchpoint to promote prespliceosome assembly...
  52. pmc Irf3 polymorphism alters induction of interferon beta in response to Listeria monocytogenes infection
    Oleg Garifulin
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Genet 3:1587-97. 2007
    ..monocytogenes infection. Thus, the C57BL/6ByJ mouse strain serves as an example of how a mammalian host can counter bacterial virulence strategies by introducing subtle alteration of noncoding sequences...
  53. pmc F-box protein FBXO31 directs degradation of MDM2 to facilitate p53-mediated growth arrest following genotoxic stress
    Sunil K Malonia
    Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605
    Proc Natl Acad Sci U S A 112:8632-7. 2015
    ..In cells depleted of FBXO31, MDM2 is not degraded and p53 levels do not increase following genotoxic stress. Thus, FBXO31 is essential for the classic robust increase in p53 levels following DNA damage. ..
  54. pmc Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors
    Ashish Misra
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 58:819-31. 2015
    ..Collectively, our results reveal an unanticipated role for mRNA 3' end formation factors in global promotion of alternative splicing. ..
  55. pmc The Blk pathway functions as a tumor suppressor in chronic myeloid leukemia stem cells
    Haojian Zhang
    Department of Medicine, Division of Hematology Oncology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Nat Genet 44:861-71. 2012
    ..Blk also suppresses the proliferation of human CML stem cells. Our results show the feasibility of selectively targeting LSCs, an approach that should be applicable to other cancers...
  56. ncbi request reprint Facioscapulohumeral muscular dystrophy in mice overexpressing FRG1
    Davide Gabellini
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 439:973-7. 2006
    ..Collectively, our results suggest that FSHD results from inappropriate overexpression of FRG1 in skeletal muscle, which leads to abnormal alternative splicing of specific pre-mRNAs...
  57. ncbi request reprint Inappropriate gene activation in FSHD: a repressor complex binds a chromosomal repeat deleted in dystrophic muscle
    Davide Gabellini
    Howard Hughes Medical Institute, Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 110:339-48. 2002
    ..Based upon these results, we propose that deletion of D4Z4 leads to the inappropriate transcriptional derepression of 4q35 genes resulting in disease...
  58. pmc Resistance to therapy in BRCA2 mutant cells due to loss of the nucleosome remodeling factor CHD4
    Shawna Guillemette
    Department of Molecular, Cell, and Cancer Biology, University of Massachusetts Medical School, Women s Cancers Program, UMASS Memorial Cancer Center, Worcester, Massachusetts 01605, USA
    Genes Dev 29:489-94. 2015
    ..Collectively, our findings indicate that CHD4 modulates therapeutic response in BRCA2 mutant cancer cells. ..
  59. pmc Differential requirement of SAGA components for recruitment of TATA-box-binding protein to promoters in vivo
    Sukesh R Bhaumik
    Howard Hughes Medical Institute, Programs in Gene Expression and Function and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell Biol 22:7365-71. 2002
    ..In summary, we show that SAGA-dependent promoters require different combinations of SAGA components for TBP recruitment, revealing a complex combinatorial network for transcription activation in vivo...
  60. pmc Transcriptional program of apoptosis induction following interleukin 2 deprivation: identification of RC3, a calcium/calmodulin binding protein, as a novel proapoptotic factor
    Laxminarayana R Devireddy
    Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell Biol 23:4532-41. 2003
    ..Based upon these results, we propose that IL-2 deprivation raises the level of RC3 and other apoptotic factors, which induce apoptosis by increasing the intracellular Ca(2+) concentration...
  61. ncbi request reprint Transcriptional derepression as a cause of genetic diseases
    Davide Gabellini
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Curr Opin Genet Dev 13:239-45. 2003
    ..The putative target genes, whose inappropriate expression is thought to cause disease, have remained elusive but are being searched for intensively...
  62. pmc Inhibition of apoptosis by ATFx: a novel role for a member of the ATF/CREB family of mammalian bZIP transcription factors
    Stephan P Persengiev
    Howard Hughes Medical Institute and Programs in Molecular Medicine and Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 16:1806-14. 2002
    ..However, constitutive expression of ATFx renders cells resistant to 24p3-mediated apoptosis. Collectively, our results indicate that ATFx is an anti-apoptotic factor, a novel role for an ATF protein...
  63. ncbi request reprint Interaction of Gal4p with components of transcription machinery in vivo
    Sukesh R Bhaumik
    Department of Biochemistry and Molecular Biology, Southern Illinois University, Carbondale, Illinois 62901, USA
    Methods Enzymol 370:445-54. 2003
  64. ncbi request reprint Cell motility is controlled by SF2/ASF through alternative splicing of the Ron protooncogene
    Claudia Ghigna
    Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, Pavia, Italy
    Mol Cell 20:881-90. 2005
    ..This demonstrates a direct link between SF2/ASF-regulated splicing and cell motility, an activity important for embryogenesis, tissue formation, and tumor metastasis...

Research Grants3

  1. TRANSCRIPTION ACTIVATION BY HIV, TAT, HTLV TAX & HBV PX
    Michael Green; Fiscal Year: 2004
    ..However, BEF works by a different mechanism, as a molecular chaperone. Experiments are proposed to further study the mechanism of BEF action. ..
  2. Intracellular Localization of HIV-1 RNAs by Rev&Matrix
    Michael Green; Fiscal Year: 2005
    ..Finally, our results suggest a novel method for inhibiting the activity of any mRNA. This "mRNA nuclear entrapment" strategy will be developed and tested using HIV-1 replication as a model system. ..
  3. Role of Lipocalin 24p3 in Apoptosis and Leukemia
    Michael R Green; Fiscal Year: 2010
    ..We have derived 24p3 homozygous knockout mice, which will be used to study the contribution of the 24p3/24p3R proapoptotic pathway to BCR/ABL-induced myeloproliferative disease. ..