Richard Gourse

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc CoSMoS unravels mysteries of transcription initiation
    Richard L Gourse
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Cell 148:635-7. 2012
  2. pmc Suppression of a dnaKJ deletion by multicopy dksA results from non-feedback-regulated transcripts that originate upstream of the major dksA promoter
    Pete Chandrangsu
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Bacteriol 194:1437-46. 2012
  3. pmc General pathway for turning on promoters transcribed by RNA polymerases containing alternative sigma factors
    Richard L Gourse
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA
    J Bacteriol 188:4589-91. 2006
  4. ncbi request reprint rRNA transcription and growth rate-dependent regulation of ribosome synthesis in Escherichia coli
    R L Gourse
    Department of Bacteriology, University of Wisconsin, Madison 53706, USA
    Annu Rev Microbiol 50:645-77. 1996
  5. ncbi request reprint Magic spots cast a spell on DNA primase
    Richard L Gourse
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, Madison, WI 53706, USA
    Cell 128:823-4. 2007
  6. ncbi request reprint UPs and downs in bacterial transcription initiation: the role of the alpha subunit of RNA polymerase in promoter recognition
    R L Gourse
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 37:687-95. 2000
  7. ncbi request reprint Mechanism of regulation of transcription initiation by ppGpp. II. Models for positive control based on properties of RNAP mutants and competition for RNAP
    M M Barker
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI, 53706, USA
    J Mol Biol 305:689-702. 2001
  8. ncbi request reprint Mechanism of regulation of transcription initiation by ppGpp. I. Effects of ppGpp on transcription initiation in vivo and in vitro
    M M Barker
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI, 53706, USA
    J Mol Biol 305:673-88. 2001
  9. pmc Both fis-dependent and factor-independent upstream activation of the rrnB P1 promoter are face of the helix dependent
    J T Newlands
    Department of Bacteriology, University of Wisconsin, Madison 53706
    Nucleic Acids Res 20:719-26. 1992
  10. pmc DNA binding and bending are necessary but not sufficient for Fis-dependent activation of rrnB P1
    K K Gosink
    Department of Bacteriology, University of Wisconsin, Madison 53706
    J Bacteriol 175:1580-9. 1993

Collaborators

  • Wilma Ross
  • Michael S Bartlett
  • S E Finkel
  • R R Burgess
  • J Alex Appleman
  • Sarah E Ades
  • John Foster
  • Dmitry G Vassylyev
  • Nigel J Savery
  • Brian J Paul
  • David A Schneider
  • Tamas Gaal
  • Shanil P Haugen
  • Heath D Murray
  • Catherine E Vrentas
  • Steven T Rutherford
  • M M Barker
  • Sarah E Aiyar
  • T Gaal
  • Pete Chandrangsu
  • Alessandra Costanzo
  • Sarah M McLeod
  • Patrick F Suthers
  • Melanie B Berkmen
  • Prabhat Mallik
  • Mariam Quinones
  • Libor Krásný
  • Neil R Stokes
  • Melicent C Peck
  • Reid C Johnson
  • C A Hirvonen
  • Sang Ho Choi
  • Li Wang
  • Michele Manrique
  • Sarah E Barchinger
  • Herve Nicoloff
  • Amy B Banta
  • Justin J Lemke
  • John Yin
  • Harvey H Kimsey
  • Mark J Mandel
  • Christopher Ward
  • Matthew K Waldor
  • Robert Osuna
  • Thomas J Silhavy
  • M Kainz
  • C A Josaitis
  • Richard H Ebright
  • Wendy Bartlett
  • Petra Louis
  • Ian R Booth
  • Chandrasekaran Subramaniam
  • Samantha Miller
  • Robert F Fisher
  • Sharon R Long
  • Mark S Thomas
  • Christine A Hirvonen
  • C E Wozniak
  • S E Aiyar
  • S T Estrem
  • L H Nguyen
  • V H Newburn
  • J R Anthony
  • E Marasco
  • L Rao
  • S Leirmo
  • K K Gosink
  • J T Newlands
  • M T Record
  • P J Schlax
  • R Osuna
  • R C Johnson

Detail Information

Publications51

  1. pmc CoSMoS unravels mysteries of transcription initiation
    Richard L Gourse
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Cell 148:635-7. 2012
    ..Ultimately, CoSMoS could greatly aid the study of the effects of DNA sequence and transcription factors on both prokaryotic and eukaryotic promoters...
  2. pmc Suppression of a dnaKJ deletion by multicopy dksA results from non-feedback-regulated transcripts that originate upstream of the major dksA promoter
    Pete Chandrangsu
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Bacteriol 194:1437-46. 2012
    ....
  3. pmc General pathway for turning on promoters transcribed by RNA polymerases containing alternative sigma factors
    Richard L Gourse
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA
    J Bacteriol 188:4589-91. 2006
  4. ncbi request reprint rRNA transcription and growth rate-dependent regulation of ribosome synthesis in Escherichia coli
    R L Gourse
    Department of Bacteriology, University of Wisconsin, Madison 53706, USA
    Annu Rev Microbiol 50:645-77. 1996
    ..The mechanisms contributing to rRNA transcription work together and compensate for one another when individual systems are rendered inoperative...
  5. ncbi request reprint Magic spots cast a spell on DNA primase
    Richard L Gourse
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, Madison, WI 53706, USA
    Cell 128:823-4. 2007
    ..2007) show that (p)ppGpp also inhibits DNA replication elongation by interfering with DNA primase activity. Halting replication may help cells to maintain genomic integrity during periods of transient nutrient limitation...
  6. ncbi request reprint UPs and downs in bacterial transcription initiation: the role of the alpha subunit of RNA polymerase in promoter recognition
    R L Gourse
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 37:687-95. 2000
    ..e. in the absence of proteins other than RNA polymerase) and activated transcription initiation (i.e. when stimulated by transcription factors)...
  7. ncbi request reprint Mechanism of regulation of transcription initiation by ppGpp. II. Models for positive control based on properties of RNAP mutants and competition for RNAP
    M M Barker
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI, 53706, USA
    J Mol Biol 305:689-702. 2001
    ..Our data also place considerable constraints on models invoking hypothetical factors that might increase amino acid promoter activity in a ppGpp-dependent fashion...
  8. ncbi request reprint Mechanism of regulation of transcription initiation by ppGpp. I. Effects of ppGpp on transcription initiation in vivo and in vitro
    M M Barker
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI, 53706, USA
    J Mol Biol 305:673-88. 2001
    ..The accompanying paper evaluates potential models for positive control of amino acid promoters by ppGpp that might explain the requirement of ppGpp for amino acid prototrophy...
  9. pmc Both fis-dependent and factor-independent upstream activation of the rrnB P1 promoter are face of the helix dependent
    J T Newlands
    Department of Bacteriology, University of Wisconsin, Madison 53706
    Nucleic Acids Res 20:719-26. 1992
    ..We also demonstrate that RNAP interacts with the -53 region of the rrnB P1 UAR even when these sequences are displaced upstream of the RNAP binding site, and that these interactions correlate with factor-independent activation...
  10. pmc DNA binding and bending are necessary but not sufficient for Fis-dependent activation of rrnB P1
    K K Gosink
    Department of Bacteriology, University of Wisconsin, Madison 53706
    J Bacteriol 175:1580-9. 1993
    ..Rather, the data are most easily interpreted in terms of transcription activation by direct interactions between Fis and RNA polymerase, requiring precise positioning of the two proteins facilitated by bending of the DNA binding site...
  11. ncbi request reprint Factor independent activation of rrnB P1. An "extended" promoter with an upstream element that dramatically increases promoter strength
    L Rao
    Department of Bacteriology, University of Wisconsin, Madison 53706
    J Mol Biol 235:1421-35. 1994
    ..A functional UP element is not absolutely essential for stimulation of rrnB P1 by the Fis protein...
  12. pmc Fine structure of E. coli RNA polymerase-promoter interactions: alpha subunit binding to the UP element minor groove
    W Ross
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Genes Dev 15:491-506. 2001
    ..These studies greatly improve the resolution of our picture of the promoter-RNAP interaction...
  13. pmc E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo
    W Ross
    Department of Bacteriology, University of Wisconsin, Madison 53706
    EMBO J 9:3733-42. 1990
    ..On the basis of these results, we propose roles for Fis and the upstream activation system in rRNA synthesis...
  14. pmc Regulation of rRNA transcription correlates with nucleoside triphosphate sensing
    M M Barker
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Bacteriol 183:6315-23. 2001
    ....
  15. pmc Escherichia coli promoters with UP elements of different strengths: modular structure of bacterial promoters
    W Ross
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Bacteriol 180:5375-83. 1998
    ....
  16. ncbi request reprint Sequences upstream of the-35 hexamer of rrnB P1 affect promoter strength and upstream activation
    C A Josaitis
    Department of Bacteriology, University of Wisconsin, Madison 53706
    Biochim Biophys Acta 1050:307-11. 1990
    ..UAR function appears dependent on its position relative to the RNA polymerase binding site, suggesting that a particular spatial geometry may be necessary for Fis-dependent and/or factor-independent activation to occur...
  17. ncbi request reprint The C-terminal domain of the alpha subunit of Escherichia coli RNA polymerase is required for efficient rho-dependent transcription termination
    M Kainz
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI, 53706, USA
    J Mol Biol 284:1379-90. 1998
    ..We conclude that the alphaCTD is a target for interactions with NusA that influence both termination and pausing, but in addition it participates in rho-dependent transcription termination in a NusA-independent manner...
  18. pmc Contributions of UP elements and the transcription factor FIS to expression from the seven rrn P1 promoters in Escherichia coli
    C A Hirvonen
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Bacteriol 183:6305-14. 2001
    ..These studies have implications for the control of gene expression of unlinked multigene families...
  19. ncbi request reprint Promoter recognition and discrimination by EsigmaS RNA polymerase
    T Gaal
    Department of Bacteriology, University of Wisconsin, Madison WI 53706, USA
    Mol Microbiol 42:939-54. 2001
    ..DNA recognition by EsigmaS versus Esigma70 thus presents an alternative solution to the problem of promoter selectivity...
  20. pmc DksA potentiates direct activation of amino acid promoters by ppGpp
    Brian J Paul
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 102:7823-8. 2005
    ....
  21. pmc Response of RNA polymerase to ppGpp: requirement for the omega subunit and relief of this requirement by DksA
    Catherine E Vrentas
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Genes Dev 19:2378-87. 2005
    ..Furthermore, these results suggest that either one or both of two short segments in the beta' subunit that physically link omega to the ppGpp-binding region of the enzyme may play crucial roles in ppGpp and DksA function...
  22. ncbi request reprint rRNA promoter regulation by nonoptimal binding of sigma region 1.2: an additional recognition element for RNA polymerase
    Shanil P Haugen
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, 53706, USA
    Cell 125:1069-82. 2006
    ..2-discriminator interaction, rRNA promoters create the short-lived complex required for specific responses to the RNAP binding factors ppGpp and DksA, ultimately accounting for regulation of ribosome synthesis...
  23. pmc DksA is required for growth phase-dependent regulation, growth rate-dependent control, and stringent control of fis expression in Escherichia coli
    Prabhat Mallik
    Department of Biological Sciences, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA
    J Bacteriol 188:5775-82. 2006
    ..This work extends our understanding of the multiple factors controlling fis expression and demonstrates the generality of the DksA requirement for regulation of kinetically similar promoters...
  24. pmc Crl facilitates RNA polymerase holoenzyme formation
    Tamas Gaal
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, Madison, WI 53706, USA
    J Bacteriol 188:7966-70. 2006
    ..Our results suggest that Crl facilitates holoenzyme formation, the first positive regulator identified with this mechanism of action...
  25. ncbi request reprint LexA represses CTXphi transcription by blocking access of the alpha C-terminal domain of RNA polymerase to promoter DNA
    Mariam Quinones
    Department of Molecular Microbiology, Tufts University School of Medicine and The Howard Hughes Medical Institute, Boston, Massachusetts 02111, USA
    J Biol Chem 281:39407-12. 2006
    ..To our knowledge, this is the first report of a repressor blocking transcription initiation by occluding a promoter UP element...
  26. pmc Effects of DksA, GreA, and GreB on transcription initiation: insights into the mechanisms of factors that bind in the secondary channel of RNA polymerase
    Steven T Rutherford
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, Madison, WI 53706, USA
    J Mol Biol 366:1243-57. 2007
    ..Our results provide important clues to the mechanisms of both negative and positive control of transcription initiation by DksA...
  27. ncbi request reprint Rapid responses of ribosomal RNA synthesis to nutrient shifts
    Patrick F Suthers
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, WI 53706 1607, USA
    Biotechnol Bioeng 97:1230-45. 2007
    ..Inclusion of DksA was essential in all three cases. Our work provides a foundation for using data-driven computational models to predict the kinetics of in vivo transcriptional responses...
  28. pmc Changes in Escherichia coli rRNA promoter activity correlate with changes in initiating nucleoside triphosphate and guanosine 5' diphosphate 3'-diphosphate concentrations after induction of feedback control of ribosome synthesis
    David A Schneider
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Bacteriol 185:6185-91. 2003
    ..In contrast, we observed no change in the level of DNA supercoiling under the feedback conditions examined...
  29. ncbi request reprint ppGpp and DksA likely regulate the activity of the extracytoplasmic stress factor sigmaE in Escherichia coli by both direct and indirect mechanisms
    Alessandra Costanzo
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Mol Microbiol 67:619-32. 2008
    ..However, ppGpp and its cofactor DksA can also activate transcription by EsigmaEin vitro, suggesting that the effects of ppGpp on sigmaE activity are both direct and indirect...
  30. pmc Still looking for the magic spot: the crystallographically defined binding site for ppGpp on RNA polymerase is unlikely to be responsible for rRNA transcription regulation
    Catherine E Vrentas
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    J Mol Biol 377:551-64. 2008
    ..coli ribosomal RNA transcription initiation and highlight the importance of inclusion of omega in bacterial RNAP preparations...
  31. pmc Fine structure of the promoter-sigma region 1.2 interaction
    Shanil P Haugen
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 105:3292-7. 2008
    ..2. Our results refine models of RNAP-DNA interactions in the promoter complex that are crucial for regulation of transcription initiation...
  32. pmc Advances in bacterial promoter recognition and its control by factors that do not bind DNA
    Shanil P Haugen
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, Wisconsin 53706, USA
    Nat Rev Microbiol 6:507-19. 2008
    ..These factors can target promoters by affecting specific kinetic steps on the pathway to open complex formation, thereby regulating RNA output from specific promoters...
  33. pmc Sequence-independent upstream DNA-alphaCTD interactions strongly stimulate Escherichia coli RNA polymerase-lacUV5 promoter association
    Wilma Ross
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 102:291-6. 2005
    ....
  34. ncbi request reprint rRNA transcription in Escherichia coli
    Brian J Paul
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Annu Rev Genet 38:749-70. 2004
    ..Together, these advances provide clues important for our molecular understanding not only of rRNA transcription, but also of transcription in general...
  35. pmc An alternative strategy for bacterial ribosome synthesis: Bacillus subtilis rRNA transcription regulation
    Libor Krásný
    Department of Bacteriology, University of Wisconsin, Madison, WI, USA
    EMBO J 23:4473-83. 2004
    ..subtilis RNA polymerase directly. Rather, increases in the ppGpp concentration might reduce the available GTP pools, thereby modulating rRNA promoter activity indirectly...
  36. ncbi request reprint Architecture of Fis-activated transcription complexes at the Escherichia coli rrnB P1 and rrnE P1 promoters
    Sarah E Aiyar
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706 1567, USA
    J Mol Biol 316:501-16. 2002
    ..Thus, similar Fis-alphaCTD interactions are used for activation of transcription when the activator is bound at very different positions on the DNA...
  37. ncbi request reprint The C-terminal domains of the RNA polymerase alpha subunits: contact site with Fis and localization during co-activation with CRP at the Escherichia coli proP P2 promoter
    Sarah M McLeod
    Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, CA 90095 1737, USA
    J Mol Biol 316:517-29. 2002
    ..These experiments define the overall architecture of the proP P2 initiation complex where Fis and CRP each function through a different alphaCTD...
  38. pmc Determinants of the C-terminal domain of the Escherichia coli RNA polymerase alpha subunit important for transcription at class I cyclic AMP receptor protein-dependent promoters
    Nigel J Savery
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, United Kingdom
    J Bacteriol 184:2273-80. 2002
    ..We also conclude that the relative contributions of individual residues within the 265 determinant depend on promoter sequence, and we discuss explanations for effects of substitutions in the 261 determinant...
  39. pmc NTP-sensing by rRNA promoters in Escherichia coli is direct
    David A Schneider
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706 1567, USA
    Proc Natl Acad Sci U S A 99:8602-7. 2002
    ..We conclude that translation is a major consumer of purine NTPs, and that NTP-sensing by rrn P1 promoters serves as a direct regulatory link between translation and ribosome synthesis...
  40. pmc Regulation of the Escherichia coli rrnB P2 promoter
    Heath D Murray
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706, USA
    J Bacteriol 185:28-34. 2003
    ..The rrnB P2 core promoter sequence (-37 to +7) is sufficient to serve as the target for growth rate-dependent regulation...
  41. ncbi request reprint Control of rRNA expression in Escherichia coli
    David A Schneider
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706, USA
    Curr Opin Microbiol 6:151-6. 2003
    ..Recent work has dissected many of the molecular mechanisms responsible for the strength and regulation of rRNA promoters...
  42. pmc An intersubunit contact stimulating transcription initiation by E coli RNA polymerase: interaction of the alpha C-terminal domain and sigma region 4
    Wilma Ross
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Genes Dev 17:1293-307. 2003
    ..Previous data from the literature suggest that this same sigma-alphaCTD interaction also plays a role in transcription factor-mediated activation...
  43. ncbi request reprint Control of rRNA expression by small molecules is dynamic and nonredundant
    Heath D Murray
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA
    Mol Cell 12:125-34. 2003
    ..The results suggest a molecular logic for the use of two homeostatic regulatory mechanisms to monitor different aspects of ribosome activity and provide general insights into the nature of overlapping regulatory circuits...
  44. pmc Changes in the concentrations of guanosine 5'-diphosphate 3'-diphosphate and the initiating nucleoside triphosphate account for inhibition of rRNA transcription in fructose-1,6-diphosphate aldolase (fda) mutants
    David A Schneider
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Bacteriol 185:6192-4. 2003
    ....
  45. pmc A role for mechanosensitive channels in survival of stationary phase: regulation of channel expression by RpoS
    Neil R Stokes
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 100:15959-64. 2003
    ..These data suggest that MscS and MscL are components of the RpoS regulon and play an important role in ensuring structural integrity in stationary phase bacteria...
  46. ncbi request reprint Measuring control of transcription initiation by changing concentrations of nucleotides and their derivatives
    David A Schneider
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, Wisconsin 53706 1567, USA
    Methods Enzymol 370:606-17. 2003
  47. ncbi request reprint Unique roles of the rrn P2 rRNA promoters in Escherichia coli
    Heath D Murray
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA
    Mol Microbiol 52:1375-87. 2004
    ..Our work significantly extends previous information about the regulators responsible for control of the rrn P2 promoters and the relationship between the tandem rRNA promoters...
  48. ncbi request reprint DksA: a critical component of the transcription initiation machinery that potentiates the regulation of rRNA promoters by ppGpp and the initiating NTP
    Brian J Paul
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison 53706, USA
    Cell 118:311-22. 2004
    ....
  49. pmc rRNA promoter activity in the fast-growing bacterium Vibrio natriegens
    Sarah E Aiyar
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    J Bacteriol 184:1349-58. 2002
    ..coli, i.e., high gene dose and strong activation by UP elements and Fis...
  50. ncbi request reprint Relationship between growth rate and ATP concentration in Escherichia coli: a bioassay for available cellular ATP
    David A Schneider
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 279:8262-8. 2004
    ..More broadly, the luciferase bioassay described here provides a general method for evaluating the ATP concentration available for biochemical processes in E. coli and potentially in other organisms...
  51. pmc The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro
    Melicent C Peck
    Department of Biological Sciences, Stanford University, California 94305, USA
    J Bacteriol 184:3808-14. 2002
    ..The ability to utilize E. coli as a heterologous system in which to study the regulation of S. meliloti genes could provide an important tool for our understanding and manipulation of these processes...

Research Grants21

  1. MECHANISM, ACTIVATION AND CONTROL OF RRNA TRANSCRIPTION
    Richard Gourse; Fiscal Year: 2002
    ..We now propose to begin to determine the relationship(s) between the multiple mechanisms affecting regulation of the synthesis of the translation apparatus during steady state growth, upshifts, and in stationary phase. ..
  2. MECHANISM, ACTIVATION, AND CONTROL OF RRNA TRANSCRIPTION
    Richard Gourse; Fiscal Year: 2006
    ..In the fourth specific aim, we will use the tools of cell biology to determine the locations of rRNA operons in the bacterial nucleoid. ..
  3. MECHANISM, ACTIVATION, AND CONTROL OF RRNA TRANSCRIPTION
    Richard Gourse; Fiscal Year: 2007
    ....
  4. MECHANISM, ACTIVATION, & CONTROL OF RRNA TRANSCRIPTION
    Richard Gourse; Fiscal Year: 1993
    ....