Tamir Gonen

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. doi request reprint Polymorphic assemblies and crystalline arrays of lens tetraspanin MP20
    Tamir Gonen
    School of Biological Sciences, University of Auckland, Auckland, New Zealand
    J Mol Biol 376:380-92. 2008
  2. ncbi request reprint The structure of aquaporins
    Tamir Gonen
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Q Rev Biophys 39:361-96. 2006
  3. pmc The Ndc80 kinetochore complex directly modulates microtubule dynamics
    Neil T Umbreit
    Department of Biochemistry, Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 109:16113-8. 2012
  4. ncbi request reprint Accurate design of co-assembling multi-component protein nanomaterials
    Neil P King
    1 Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA 2 Institute for Protein Design, University of Washington, Seattle, Washington 98195, USA 3
    Nature 510:103-8. 2014
  5. pmc AKAP2 anchors PKA with aquaporin-0 to support ocular lens transparency
    Matthew G Gold
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA, USA
    EMBO Mol Med 4:15-26. 2012
  6. pmc The binding of cholera toxin to the periplasmic vestibule of the type II secretion channel
    Steve L Reichow
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Channels (Austin) 5:215-8. 2011
  7. pmc Secretins: dynamic channels for protein transport across membranes
    Konstantin V Korotkov
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Trends Biochem Sci 36:433-43. 2011
  8. pmc The structure of purified kinetochores reveals multiple microtubule-attachment sites
    Shane Gonen
    Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Nat Struct Mol Biol 19:925-9. 2012
  9. pmc Tension directly stabilizes reconstituted kinetochore-microtubule attachments
    Bungo Akiyoshi
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Nature 468:576-9. 2010
  10. pmc Structure of the cholera toxin secretion channel in its closed state
    Steve L Reichow
    Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Nat Struct Mol Biol 17:1226-32. 2010

Collaborators

Detail Information

Publications27

  1. doi request reprint Polymorphic assemblies and crystalline arrays of lens tetraspanin MP20
    Tamir Gonen
    School of Biological Sciences, University of Auckland, Auckland, New Zealand
    J Mol Biol 376:380-92. 2008
    ..The various complexes formed by MP20 most likely reflect the many ways in which tetraspanins can interact with each other to allow formation of TEMs...
  2. ncbi request reprint The structure of aquaporins
    Tamir Gonen
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Q Rev Biophys 39:361-96. 2006
    ..The review will discuss the mechanisms governing water conduction, proton exclusion and substrate specificity, and how the pore permeability is regulated in different members of the AQP family...
  3. pmc The Ndc80 kinetochore complex directly modulates microtubule dynamics
    Neil T Umbreit
    Department of Biochemistry, Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 109:16113-8. 2012
    ..Taken together, these results suggest that in addition to regulating attachment stability, Aurora B controls microtubule dynamics through phosphorylation of the Ndc80 complex...
  4. ncbi request reprint Accurate design of co-assembling multi-component protein nanomaterials
    Neil P King
    1 Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA 2 Institute for Protein Design, University of Washington, Seattle, Washington 98195, USA 3
    Nature 510:103-8. 2014
    ..The accuracy of the method and the number and variety of two-component materials that it makes accessible suggest a route to the construction of functional protein nanomaterials tailored to specific applications. ..
  5. pmc AKAP2 anchors PKA with aquaporin-0 to support ocular lens transparency
    Matthew G Gold
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA, USA
    EMBO Mol Med 4:15-26. 2012
    ..Thus, anchored PKA modulation of AQP0 is a homeostatic mechanism that must be physically intact to preserve lens transparency...
  6. pmc The binding of cholera toxin to the periplasmic vestibule of the type II secretion channel
    Steve L Reichow
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Channels (Austin) 5:215-8. 2011
    ..Our studies indicate that the pentamer is captured within the large periplasmic vestibule of VcGspD. These new results agree well with our previously published studies and are in accord with a piston-driven type II secretion mechanism...
  7. pmc Secretins: dynamic channels for protein transport across membranes
    Konstantin V Korotkov
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Trends Biochem Sci 36:433-43. 2011
    ..Secretins might also play a key role in the biogenesis of their cognate secretion systems...
  8. pmc The structure of purified kinetochores reveals multiple microtubule-attachment sites
    Shane Gonen
    Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Nat Struct Mol Biol 19:925-9. 2012
    ..Our data, showing that kinetochores bind to microtubules via multivalent attachments, lay the foundation to uncover the key mechanical and regulatory mechanisms by which kinetochores control chromosome segregation and cell division...
  9. pmc Tension directly stabilizes reconstituted kinetochore-microtubule attachments
    Bungo Akiyoshi
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Nature 468:576-9. 2010
    ..On the basis of these findings, we propose that tension selectively stabilizes proper kinetochore-microtubule attachments in vivo through a combination of direct mechanical stabilization and tension-dependent phosphoregulation...
  10. pmc Structure of the cholera toxin secretion channel in its closed state
    Steve L Reichow
    Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Nat Struct Mol Biol 17:1226-32. 2010
    ..By combining our results with structures of the cholera toxin and T2SS pseudopilus tip, we provide a structural basis for a possible secretion mechanism of the T2SS...
  11. pmc Intrinsic tethering activity of endosomal Rab proteins
    Sheng Ying Lo
    Department of Biochemistry, University of Washington School of Medicine, Seattle, Washington, USA
    Nat Struct Mol Biol 19:40-7. 2012
    ..In our working model, the intrinsic tethering capacity Vps21 operates in concert with conventional effectors and SNAREs to drive efficient docking and fusion...
  12. pmc The prototypical H+/galactose symporter GalP assembles into functional trimers
    Hongjin Zheng
    Department of Biochemistry, University of Washington, 1705 NE Pacific Street, Seattle, WA 98195, USA
    J Mol Biol 396:593-601. 2010
    ..We show that the crystalline GalP is able to selectively bind substrate, suggesting that the trimeric form is biologically active...
  13. pmc Lipid-protein interactions probed by electron crystallography
    Steve L Reichow
    Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195 7350, USA
    Curr Opin Struct Biol 19:560-5. 2009
    ..Lipids are tightly integrated into the protein's architecture where they can affect the function, structure, quaternary assembly, and the stability of the membrane protein...
  14. pmc Haemolysin coregulated protein is an exported receptor and chaperone of type VI secretion substrates
    Julie M Silverman
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Mol Cell 51:584-93. 2013
    ..These unique functions of a secreted protein highlight fundamental differences between the export mechanism of T6 and other characterized secretory pathways. ..
  15. doi request reprint Computational design of self-assembling protein nanomaterials with atomic level accuracy
    Neil P King
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Science 336:1171-4. 2012
    ..The method can be used to design a wide variety of self-assembling protein nanomaterials...
  16. pmc Fragment-based phase extension for three-dimensional structure determination of membrane proteins by electron crystallography
    Goragot Wisedchaisri
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Structure 19:976-87. 2011
    ..Using this approach, structures of three membrane proteins were determined rapidly and accurately to atomic resolution without high-resolution image data...
  17. pmc Electron crystallography of aquaporins
    Simeon Andrews
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    IUBMB Life 60:430-6. 2008
    ....
  18. pmc Noncanonical binding of calmodulin to aquaporin-0: implications for channel regulation
    Steve L Reichow
    Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195 7350, USA
    Structure 16:1389-98. 2008
    ..Finally, phosphorylation within AQP0's CaM binding domain inhibits the AQP0/CaM interaction, suggesting a temporal regulatory mechanism for complex formation...
  19. pmc Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B
    Jerry F Tien
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    J Cell Biol 189:713-23. 2010
    ..We propose that the action of the Dam1 complex as a processivity factor in kinetochore-microtubule attachment is regulated by conserved signals for error correction...
  20. pmc A conformational switch in bacteriophage p22 portal protein primes genome injection
    Hongjin Zheng
    Department of Biochemistry, University of Washington, 1705 NE Pacific Street, Seattle, WA 98195, USA
    Mol Cell 29:376-83. 2008
    ..We hypothesize that this conformational change primes dsDNA for injection and directly couples completion of virus morphogenesis to a new cycle of infection...
  21. pmc Interactions of lipids with aquaporin-0 and other membrane proteins
    Richard K Hite
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Pflugers Arch 456:651-61. 2008
    ....
  22. pmc Lipid-protein interactions in double-layered two-dimensional AQP0 crystals
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 438:633-8. 2005
    ..We were therefore able to build an atomic model for the lipid bilayer surrounding the AQP0 tetramers, and we describe lipid-protein interactions...
  23. ncbi request reprint Aquaporin-0 membrane junctions form upon proteolytic cleavage
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 342:1337-45. 2004
    ..This change of function of AQP0 from a water pore in the cortex to an adhesion molecule in the lens core constitutes another manifestation of the gene sharing concept originally proposed on the basis of the dual function of crystallins...
  24. ncbi request reprint Aquaporin-0 membrane junctions reveal the structure of a closed water pore
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 429:193-7. 2004
    ..The water pathway in AQP0 also contains an additional pore constriction, not seen in other known aquaporin structures, which may be responsible for pore gating...
  25. ncbi request reprint Insertion of MP20 into lens fibre cell plasma membranes correlates with the formation of an extracellular diffusion barrier
    Angus C Grey
    Department of Physiology, School of Medical Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
    Exp Eye Res 77:567-74. 2003
    ..Our results suggest that the insertion of MP20 into fibre cell membranes coincides with the creation of a barrier that restricts the diffusion of molecules into the lens core via the extracellular space...
  26. pmc Junction-forming aquaporins
    Andreas Engel
    Maurice E Mueller Institute, University of Basel, Biozentrum, Klingelberstrasse 70, CH 4056 Basel, Switzerland
    Curr Opin Struct Biol 18:229-35. 2008
    ..We discuss the recently determined structures of the membrane junctions mediated by these two AQPs, the mechanisms that regulate junction formation, and evidence that supports a role for AQP0 and AQP4 in cell adhesion...
  27. pmc Projection map of aquaporin-9 at 7 A resolution
    Hector Viadiu
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Mol Biol 367:80-8. 2007
    ....