Research Topics
Genomes and Genes | Tamir GonenSummaryAffiliation: University of Washington Country: USA Publications
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Publications
Polymorphic assemblies and crystalline arrays of lens tetraspanin MP20Tamir Gonen
School of Biological Sciences, University of Auckland, Auckland, New Zealand
J Mol Biol 376:380-92. 2008..The various complexes formed by MP20 most likely reflect the many ways in which tetraspanins can interact with each other to allow formation of TEMs...
The structure of aquaporinsTamir Gonen
Department of Biochemistry, University of Washington, Seattle, WA, USA
Q Rev Biophys 39:361-96. 2006..The review will discuss the mechanisms governing water conduction, proton exclusion and substrate specificity, and how the pore permeability is regulated in different members of the AQP family...
The Ndc80 kinetochore complex directly modulates microtubule dynamicsNeil T Umbreit
Department of Biochemistry, Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 109:16113-8. 2012..Taken together, these results suggest that in addition to regulating attachment stability, Aurora B controls microtubule dynamics through phosphorylation of the Ndc80 complex...- AKAP2 anchors PKA with aquaporin-0 to support ocular lens transparencyMatthew G Gold
Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA, USA
EMBO Mol Med 4:15-26. 2012..Thus, anchored PKA modulation of AQP0 is a homeostatic mechanism that must be physically intact to preserve lens transparency... - The binding of cholera toxin to the periplasmic vestibule of the type II secretion channelSteve L Reichow
Department of Biochemistry, University of Washington, Seattle, WA, USA
Channels (Austin) 5:215-8. 2011..Our studies indicate that the pentamer is captured within the large periplasmic vestibule of VcGspD. These new results agree well with our previously published studies and are in accord with a piston-driven type II secretion mechanism... - Secretins: dynamic channels for protein transport across membranesKonstantin V Korotkov
Department of Biochemistry, University of Washington, Seattle, WA, USA
Trends Biochem Sci 36:433-43. 2011..Secretins might also play a key role in the biogenesis of their cognate secretion systems... - The structure of purified kinetochores reveals multiple microtubule-attachment sitesShane Gonen
Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, Washington, USA
Nat Struct Mol Biol 19:925-9. 2012..Our data, showing that kinetochores bind to microtubules via multivalent attachments, lay the foundation to uncover the key mechanical and regulatory mechanisms by which kinetochores control chromosome segregation and cell division... - Tension directly stabilizes reconstituted kinetochore-microtubule attachmentsBungo Akiyoshi
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Nature 468:576-9. 2010..On the basis of these findings, we propose that tension selectively stabilizes proper kinetochore-microtubule attachments in vivo through a combination of direct mechanical stabilization and tension-dependent phosphoregulation... - Structure of the cholera toxin secretion channel in its closed stateSteve L Reichow
Department of Biochemistry, University of Washington, Seattle, Washington, USA
Nat Struct Mol Biol 17:1226-32. 2010..By combining our results with structures of the cholera toxin and T2SS pseudopilus tip, we provide a structural basis for a possible secretion mechanism of the T2SS... - Intrinsic tethering activity of endosomal Rab proteinsSheng Ying Lo
Department of Biochemistry, University of Washington School of Medicine, Seattle, Washington, USA
Nat Struct Mol Biol 19:40-7. 2012..In our working model, the intrinsic tethering capacity Vps21 operates in concert with conventional effectors and SNAREs to drive efficient docking and fusion... - The prototypical H+/galactose symporter GalP assembles into functional trimersHongjin Zheng
Department of Biochemistry, University of Washington, 1705 NE Pacific Street, Seattle, WA 98195, USA
J Mol Biol 396:593-601. 2010..We show that the crystalline GalP is able to selectively bind substrate, suggesting that the trimeric form is biologically active... - Lipid-protein interactions probed by electron crystallographySteve L Reichow
Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195 7350, USA
Curr Opin Struct Biol 19:560-5. 2009..Lipids are tightly integrated into the protein's architecture where they can affect the function, structure, quaternary assembly, and the stability of the membrane protein... - Computational design of self-assembling protein nanomaterials with atomic level accuracyNeil P King
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
Science 336:1171-4. 2012..The method can be used to design a wide variety of self-assembling protein nanomaterials... - Electron crystallography of aquaporinsSimeon Andrews
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
IUBMB Life 60:430-6. 2008.... - Fragment-based phase extension for three-dimensional structure determination of membrane proteins by electron crystallographyGoragot Wisedchaisri
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
Structure 19:976-87. 2011..Using this approach, structures of three membrane proteins were determined rapidly and accurately to atomic resolution without high-resolution image data... - Noncanonical binding of calmodulin to aquaporin-0: implications for channel regulationSteve L Reichow
Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195 7350, USA
Structure 16:1389-98. 2008..Finally, phosphorylation within AQP0's CaM binding domain inhibits the AQP0/CaM interaction, suggesting a temporal regulatory mechanism for complex formation... - Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora BJerry F Tien
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
J Cell Biol 189:713-23. 2010..We propose that the action of the Dam1 complex as a processivity factor in kinetochore-microtubule attachment is regulated by conserved signals for error correction... - A conformational switch in bacteriophage p22 portal protein primes genome injectionHongjin Zheng
Department of Biochemistry, University of Washington, 1705 NE Pacific Street, Seattle, WA 98195, USA
Mol Cell 29:376-83. 2008..We hypothesize that this conformational change primes dsDNA for injection and directly couples completion of virus morphogenesis to a new cycle of infection... - Aquaporin-0 membrane junctions reveal the structure of a closed water poreTamir Gonen
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 429:193-7. 2004..The water pathway in AQP0 also contains an additional pore constriction, not seen in other known aquaporin structures, which may be responsible for pore gating... - Aquaporin-0 membrane junctions form upon proteolytic cleavageTamir Gonen
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
J Mol Biol 342:1337-45. 2004..This change of function of AQP0 from a water pore in the cortex to an adhesion molecule in the lens core constitutes another manifestation of the gene sharing concept originally proposed on the basis of the dual function of crystallins... - Lipid-protein interactions in double-layered two-dimensional AQP0 crystalsTamir Gonen
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 438:633-8. 2005..We were therefore able to build an atomic model for the lipid bilayer surrounding the AQP0 tetramers, and we describe lipid-protein interactions... - Insertion of MP20 into lens fibre cell plasma membranes correlates with the formation of an extracellular diffusion barrierAngus C Grey
Department of Physiology, School of Medical Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
Exp Eye Res 77:567-74. 2003..Our results suggest that the insertion of MP20 into fibre cell membranes coincides with the creation of a barrier that restricts the diffusion of molecules into the lens core via the extracellular space... - Interactions of lipids with aquaporin-0 and other membrane proteinsRichard K Hite
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Pflugers Arch 456:651-61. 2008.... - Junction-forming aquaporinsAndreas Engel
Maurice E Mueller Institute, University of Basel, Biozentrum, Klingelberstrasse 70, CH 4056 Basel, Switzerland
Curr Opin Struct Biol 18:229-35. 2008..We discuss the recently determined structures of the membrane junctions mediated by these two AQPs, the mechanisms that regulate junction formation, and evidence that supports a role for AQP0 and AQP4 in cell adhesion... - Projection map of aquaporin-9 at 7 A resolutionHector Viadiu
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
J Mol Biol 367:80-8. 2007....