Elizabeth J Goldsmith

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi request reprint Three-dimensional docking in the MAPK p38α
    Elizabeth J Goldsmith
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 8816, USA
    Sci Signal 4:pe47. 2011
  2. pmc Substrate and docking interactions in serine/threonine protein kinases
    Elizabeth J Goldsmith
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390 8816, USA
    Chem Rev 107:5065-81. 2007
  3. ncbi request reprint Structural studies of MAP Kinase cascade components
    Elizabeth J Goldsmith
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA
    Methods Mol Biol 661:223-37. 2010
  4. ncbi request reprint WNK1: analysis of protein kinase structure, downstream targets, and potential roles in hypertension
    Bing E Xu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX, USA
    Cell Res 15:6-10. 2005
  5. pmc The structure of the MAP2K MEK6 reveals an autoinhibitory dimer
    Xiaoshan Min
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 8816, USA
    Structure 17:96-104. 2009
  6. ncbi request reprint Properties of WNK1 and implications for other family members
    Lisa Y Lenertz
    Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    J Biol Chem 280:26653-8. 2005
  7. pmc Evolution of substrate specificity within a diverse family of beta/alpha-barrel-fold basic amino acid decarboxylases: X-ray structure determination of enzymes with specificity for L-arginine and carboxynorspermidine
    Xiaoyi Deng
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 285:25708-19. 2010
  8. pmc Precisely ordered phosphorylation reactions in the p38 mitogen-activated protein (MAP) kinase cascade
    John M Humphreys
    Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 288:23322-30. 2013
  9. ncbi request reprint Crystal structure of the TAO2 kinase domain: activation and specificity of a Ste20p MAP3K
    Tianjun Zhou
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Structure 12:1891-900. 2004
  10. pmc Unique MAP Kinase binding sites
    Radha Akella
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 8816, USA
    Biochim Biophys Acta 1784:48-55. 2008

Collaborators

  • Melanie Cobb
  • Anthony J Michael
  • Margaret Phillips
  • Seung Jae Lee
  • Yan Gao
  • Thomas M Moon
  • Yan Li
  • John A Tainer
  • Kim Orth
  • Kevin H Gardner
  • Qiong Wu
  • Yong Wang
  • W Kaminsky
  • J Han
  • Min Zheng
  • MISCHA CHRISTIAN MACHIUS
  • Radha Akella
  • Xiaoshan Min
  • Bing E Xu
  • Byung Hoon Lee
  • Xiaoyi Deng
  • Alexander T Piala
  • Haixia He
  • John M Humphreys
  • Tianjun Zhou
  • Jeongmi Lee
  • Laurie K Jackson
  • Rahul Shah
  • Mustafa N Yazicioglu
  • Huamin Zhou
  • Sohini Mukherjee
  • Julie L Wilsbacher
  • Tian Jun Zhou
  • Lisa Y Lenertz
  • Svetlana Earnest
  • Charles J Heise
  • Thomas Lee
  • Steve Stippec
  • Chung I Chang
  • Diana R Tomchick
  • Ramesh Gujjar
  • Pradipsinh K Rathod
  • Susan E Tsutakawa
  • Nicholas A Malmquist
  • Farah El Mazouni
  • Dariusz Martynowski
  • Angelique W Whitehurst
  • Daryl L Goad
  • Aarati Ranganathan
  • Li guang Sun
  • Derk Binns
  • Shengcai Lin
  • Tyler Zarubin
  • Zhiyun Ye
  • Anand R Kolatkar
  • Haydn L Ball
  • Jianming Chen
  • Andrei V Khokhlatchev
  • John Humphreys
  • Changchuan Xie
  • Liguang Sun
  • Yu Chi Juang
  • Gladys Keitany
  • Ewen Gallagher
  • Kyle Wedin
  • Lisa Lenertz
  • Kate Luby-Phelps
  • Hongjun Shu
  • Lin Chen
  • Yukihito Kabuyama
  • Jeffrey Baldwin
  • She Chen
  • Andrew N Hoofnagle
  • Natalie G Ahn
  • Katheryn A Resing
  • Malavika Raman
  • James Stroud
  • Zhu Chen

Detail Information

Publications33

  1. ncbi request reprint Three-dimensional docking in the MAPK p38α
    Elizabeth J Goldsmith
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 8816, USA
    Sci Signal 4:pe47. 2011
    ..This structure offers insights into the action of MKP5 and other MKPs...
  2. pmc Substrate and docking interactions in serine/threonine protein kinases
    Elizabeth J Goldsmith
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390 8816, USA
    Chem Rev 107:5065-81. 2007
  3. ncbi request reprint Structural studies of MAP Kinase cascade components
    Elizabeth J Goldsmith
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA
    Methods Mol Biol 661:223-37. 2010
    ..Here, we describe the preparation of MAP2K MEK6 and MAP3K TAO2 substituted with selenomethionine (SeMet) for de novo phasing. TAO2 and SeMet TAO2 were expressed in insect cells...
  4. ncbi request reprint WNK1: analysis of protein kinase structure, downstream targets, and potential roles in hypertension
    Bing E Xu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX, USA
    Cell Res 15:6-10. 2005
    ..The WNK kinases may be able to influence ion homeostasis through its effects on synaptotagmin function...
  5. pmc The structure of the MAP2K MEK6 reveals an autoinhibitory dimer
    Xiaoshan Min
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 8816, USA
    Structure 17:96-104. 2009
    ..The autoinhibited structure likely confers specificity on active MAP2Ks. The dimer may also serve the function in unphosphorylated MEK6 of preventing activation loop phosphorylation by inappropriate kinases...
  6. ncbi request reprint Properties of WNK1 and implications for other family members
    Lisa Y Lenertz
    Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    J Biol Chem 280:26653-8. 2005
    ..However, WNK1 phosphorylated both WNK4 and WNK2. In addition, the WNK1 autoinhibitory domain inhibited the catalytic activity of these WNKs. These findings suggest potential mechanisms for interconnected regulation of WNK family members...
  7. pmc Evolution of substrate specificity within a diverse family of beta/alpha-barrel-fold basic amino acid decarboxylases: X-ray structure determination of enzymes with specificity for L-arginine and carboxynorspermidine
    Xiaoyi Deng
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 285:25708-19. 2010
    ..These studies provide insight into the structural basis for the evolution of novel function within a common structural-fold...
  8. pmc Precisely ordered phosphorylation reactions in the p38 mitogen-activated protein (MAP) kinase cascade
    John M Humphreys
    Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 288:23322-30. 2013
    ..The models confirmed the reaction order, revealed processivity in the phosphorylation of MEK6 by ASK1, and suggested that the order of phosphorylation is dictated by both binding and catalysis rates. ..
  9. ncbi request reprint Crystal structure of the TAO2 kinase domain: activation and specificity of a Ste20p MAP3K
    Tianjun Zhou
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Structure 12:1891-900. 2004
    ..Finally, active TAO2 displays unusual interactions with ATP, involving, in part, a subgroup-specific C-terminal extension of TAO2. The observed interactions may be useful in making specific inhibitors of TAO kinases...
  10. pmc Unique MAP Kinase binding sites
    Radha Akella
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 8816, USA
    Biochim Biophys Acta 1784:48-55. 2008
    ..Crystallographic evidence of these latter two binding sites is presented...
  11. ncbi request reprint Crystallization of MAP kinases
    Seung Jae Lee
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 8816, USA
    Methods 40:224-33. 2006
    ..Further, some success has been gained in crystallizing the MAP kinase activators MAP2Ks and MAP3K kinase domains. This review describes the key methods that have been utilized to crystallize MAP kinases and MAP kinase pathway components...
  12. pmc Structural plasticity of malaria dihydroorotate dehydrogenase allows selective binding of diverse chemical scaffolds
    Xiaoyi Deng
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9041, USA
    J Biol Chem 284:26999-7009. 2009
    ..Together, these studies will directly impact efforts to exploit PfDHODH for the development of anti-malarial chemotherapy...
  13. pmc Crystal structure of domain-swapped STE20 OSR1 kinase domain
    Seung Jae Lee
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 75390 9041, USA
    Protein Sci 18:304-13. 2009
    ..The OSR1 kinase domain has now been added to a growing list of domain-swapped protein kinases recently reported, suggesting that the domain-swapping event provides an additional layer of complexity in regulating protein kinase activity...
  14. ncbi request reprint WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding
    Byung Hoon Lee
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Mol Cell 15:741-51. 2004
    ..These findings provide a biochemical mechanism that could lead to the retention or insertion of proteins in the plasma membrane. Interruption of this regulatory pathway may disturb membrane events that regulate ion balance...
  15. ncbi request reprint Crystal structure of the MAP3K TAO2 kinase domain bound by an inhibitor staurosporine
    Tian Jun Zhou
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9038, USA
    Acta Biochim Biophys Sin (Shanghai) 38:385-92. 2006
    ..The structure presented here should aid in the design of inhibitors specific to TAO2 and related kinases...
  16. ncbi request reprint Docking interactions induce exposure of activation loop in the MAP kinase ERK2
    Tianjun Zhou
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Structure 14:1011-9. 2006
    ..However, the binding interactions and conformational changes are unique to each, thus contributing to specificity among MAP kinases...
  17. ncbi request reprint Crystal structure of the kinase domain of WNK1, a kinase that causes a hereditary form of hypertension
    Xiaoshan Min
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Structure 12:1303-11. 2004
    ..The structure of the WNK1 catalytic domain, with its unique active site, may help in the design of therapeutic reagents for the treatment of hypertension...
  18. ncbi request reprint Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation
    Sohini Mukherjee
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 312:1211-4. 2006
    ..The acetylation on MAPKK6 directly competed with phosphorylation, preventing activation of the modified protein. This covalent modification may be used as a general regulatory mechanism in biological signaling...
  19. ncbi request reprint Chloride sensing by WNK1 involves inhibition of autophosphorylation
    Alexander T Piala
    1Department of Biophysics, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Sci Signal 7:ra41. 2014
    ..Thus, these data suggest that WNK1 functions as a chloride sensor through direct binding of a regulatory chloride ion to the active site, which inhibits autophosphorylation. ..
  20. pmc X-ray structure of Paramecium bursaria Chlorella virus arginine decarboxylase: insight into the structural basis for substrate specificity
    Rahul Shah
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    Biochemistry 46:2831-41. 2007
    ..In conjunction with prior structural studies these data predict that this loop adopts different conformations throughout the catalytic cycle, and that loop movement may be kinetically linked to the rate-limiting step of product release...
  21. ncbi request reprint Multiple active site conformations revealed by distant site mutation in ornithine decarboxylase
    Laurie K Jackson
    Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9041, USA
    Biochemistry 43:12990-9. 2004
    ..These data also suggest that the structure is less constrained in the mutant enzyme. The observation of a gem-diamine intermediate provides insight into the conformational changes that occur during the ODC catalytic cycle...
  22. doi request reprint The third conformation of p38α MAP kinase observed in phosphorylated p38α and in solution
    Radha Akella
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 8816, USA
    Structure 18:1571-8. 2010
    ..The structure supports the idea that MAP kinases adopt three distinct conformations: unphosphorylated, phosphorylated, and a docking peptide-induced form...
  23. ncbi request reprint Characterization of mitogen-activated protein kinase (MAPK) dimers
    Julie L Wilsbacher
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390 9041, USA
    Biochemistry 45:13175-82. 2006
    ....
  24. ncbi request reprint Mutations in ERK2 binding sites affect nuclear entry
    Mustafa N Yazicioglu
    Departments of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9041, USA
    J Biol Chem 282:28759-67. 2007
    ..These results further support the idea that direct interactions with nucleoporins are involved in ERK2 nuclear entry and that multiple events contribute to the ligand-dependent relocalization of these protein kinases...
  25. ncbi request reprint Phylogenetic diversity and the structural basis of substrate specificity in the beta/alpha-barrel fold basic amino acid decarboxylases
    Jeongmi Lee
    Departments of Pharmacology and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    J Biol Chem 282:27115-25. 2007
    ..Our data demonstrate that there is greater structural and functional diversity in bacterial polyamine biosynthetic decarboxylases than previously suspected...
  26. ncbi request reprint Structure of MAPKs
    Elizabeth J Goldsmith
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA
    Methods Mol Biol 250:127-44. 2004
  27. ncbi request reprint X-ray structure determination of Trypanosoma brucei ornithine decarboxylase bound to D-ornithine and to G418: insights into substrate binding and ODC conformational flexibility
    Laurie K Jackson
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, 5323 Harry Hines Boulevard, TX 75390 9041, USA
    J Biol Chem 278:22037-43. 2003
    ..The disordering of residues in the active site provides a potential mechanism for inhibition by G418 and suggests that allosteric inhibition from this site is feasible...
  28. pmc Natural language query in the biochemistry and molecular biology domains based on cognition search™
    Elizabeth J Goldsmith
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390 8816
    Summit on Translat Bioinforma 2009:32-7. 2009
    ..Thus, the CSIR has the right architecture to form the basis for a scientific search engine...
  29. ncbi request reprint Another twist in helix C and a missing pocket
    Elizabeth J Goldsmith
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, 75390, USA
    Structure 10:888-9. 2002
    ..PKB/Akt reveals a major role for helix C in the regulation of activity in the first structure of an AGC family protein kinase in its low-activity form...
  30. ncbi request reprint Crystal structures of MAP kinase p38 complexed to the docking sites on its nuclear substrate MEF2A and activator MKK3b
    Chung I Chang
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Mol Cell 9:1241-9. 2002
    ..The peptides also induce unexpected and different conformational changes in the active site, as well as structural disorder in the phosphorylation lip...
  31. pmc Determinants that control the specific interactions between TAB1 and p38alpha
    Huamin Zhou
    The Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Fujian, China
    Mol Cell Biol 26:3824-34. 2006
    ..This suggests that TAB1-induced autophosphorylation of p38alpha results from conformational changes that are similar but unique to those seen in p38alpha interactions with its substrates and activating kinases...
  32. ncbi request reprint Regulation of WNK1 by an autoinhibitory domain and autophosphorylation
    Bing E Xu
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas 75390 9041, USA
    J Biol Chem 277:48456-62. 2002
    ..We also found that WNK1 expressed in bacteria is autophosphorylated; autophosphorylation on serine 382 in the activation loop is required for its activity...
  33. ncbi request reprint Docking motif interactions in MAP kinases revealed by hydrogen exchange mass spectrometry
    Thomas Lee
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309, USA
    Mol Cell 14:43-55. 2004
    ..In vitro assays confirm the dependence of Elk1 and nucleoporin binding on ERK2 phosphorylation, and provide a structural basis for preferential involvement of active ERK in substrate binding and nuclear pore protein interactions...