David Goldfarb

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. ncbi request reprint Importin alpha: a multipurpose nuclear-transport receptor
    David S Goldfarb
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Trends Cell Biol 14:505-14. 2004
  2. doi request reprint Nuclear dimorphism: two peas in a pod
    David S Goldfarb
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Curr Biol 19:R449-52. 2009
  3. doi request reprint How to grow a bud: an importin acts in asymmetric division
    David S Goldfarb
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Nat Cell Biol 11:243-5. 2009
  4. doi request reprint Evolution of the metazoan-specific importin alpha gene family
    D Adam Mason
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    J Mol Evol 68:351-65. 2009
  5. pmc Genomic instability is associated with natural life span variation in Saccharomyces cerevisiae
    Hong Qin
    Center for Aging and Development Biology, Department of Biology, University of Rochester, Rochester, New York, United States of America
    PLoS ONE 3:e2670. 2008
  6. ncbi request reprint Measuring piecemeal microautophagy of the nucleus in Saccharomyces cerevisiae
    Jonathan I Millen
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Autophagy 5:75-81. 2009
  7. pmc The luminal N-terminus of yeast Nvj1 is an inner nuclear membrane anchor
    Jonathan I Millen
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Traffic 9:1653-64. 2008
  8. ncbi request reprint Nucleus-vacuole junctions and piecemeal microautophagy of the nucleus in S. cerevisiae
    Erik Kvam
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Autophagy 3:85-92. 2007
  9. ncbi request reprint Structure and function of nucleus-vacuole junctions: outer-nuclear-membrane targeting of Nvj1p and a role in tryptophan uptake
    Erik Kvam
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    J Cell Sci 119:3622-33. 2006
  10. ncbi request reprint Atg19p ubiquitination and the cytoplasm to vacuole trafficking pathway in yeast
    Bonnie K Baxter
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    J Biol Chem 280:39067-76. 2005

Research Grants

  1. Molecular Screen for Lifespan Extension in Yeast (RMI)
    David Goldfarb; Fiscal Year: 2004
  2. High Through-Put Genomics Approach To Lifespan in Yeast
    David Goldfarb; Fiscal Year: 2006
  3. Yeast Lifespan Chemical Screening (RMI)
    David Goldfarb; Fiscal Year: 2005
  4. Gating the nuclear pore complex translocon
    David Goldfarb; Fiscal Year: 2006

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Importin alpha: a multipurpose nuclear-transport receptor
    David S Goldfarb
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Trends Cell Biol 14:505-14. 2004
    ..We also describe activities of importin alpha that seem to be distinct from its housekeeping functions in nuclear transport...
  2. doi request reprint Nuclear dimorphism: two peas in a pod
    David S Goldfarb
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Curr Biol 19:R449-52. 2009
    ..This extreme case of nuclear dimorphism can now be partially attributed to differences in the subunit compositions of their nuclear pore complexes...
  3. doi request reprint How to grow a bud: an importin acts in asymmetric division
    David S Goldfarb
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Nat Cell Biol 11:243-5. 2009
    ..An unexpected collaboration between the DEAD-box protein Dbp5 and the nuclear transport receptor Kap104 controls localized protein synthesis at the bud tip during mitosis...
  4. doi request reprint Evolution of the metazoan-specific importin alpha gene family
    D Adam Mason
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    J Mol Evol 68:351-65. 2009
    ..Finally, these results establish a rational basis for a unified nomenclature of the importin alpha gene family...
  5. pmc Genomic instability is associated with natural life span variation in Saccharomyces cerevisiae
    Hong Qin
    Center for Aging and Development Biology, Department of Biology, University of Rochester, Rochester, New York, United States of America
    PLoS ONE 3:e2670. 2008
    ..To the best of our knowledge, this is the first quantitative evidence that demonstrates a link between genomic instability and natural variation in life span...
  6. ncbi request reprint Measuring piecemeal microautophagy of the nucleus in Saccharomyces cerevisiae
    Jonathan I Millen
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Autophagy 5:75-81. 2009
    ..Together, these assays should facilitate the further study of this fascinating but poorly understood autophagic process in different genetic backgrounds, physiological states, and environmental conditions...
  7. pmc The luminal N-terminus of yeast Nvj1 is an inner nuclear membrane anchor
    Jonathan I Millen
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Traffic 9:1653-64. 2008
    ..We conclude that the N-terminus of Nvj1 is a retention sequence that bridges the perinuclear lumen and inserts into the inner nuclear membrane...
  8. ncbi request reprint Nucleus-vacuole junctions and piecemeal microautophagy of the nucleus in S. cerevisiae
    Erik Kvam
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Autophagy 3:85-92. 2007
    ..The sequestration of Osh1p by Nvj1p likely serves to negatively regulate the trafficking of tryptophan permease(s) to the plasma membrane. Thus, NV junctions and PMN orchestrate novel and sophisticated responses to nutrient limitation...
  9. ncbi request reprint Structure and function of nucleus-vacuole junctions: outer-nuclear-membrane targeting of Nvj1p and a role in tryptophan uptake
    Erik Kvam
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    J Cell Sci 119:3622-33. 2006
    ..Therefore, the Nvj1p-dependent sequestration of Osh1p negatively regulates tryptophan uptake from the medium, possible by affecting the trafficking of tryptophan permeases to the plasma membrane...
  10. ncbi request reprint Atg19p ubiquitination and the cytoplasm to vacuole trafficking pathway in yeast
    Bonnie K Baxter
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    J Biol Chem 280:39067-76. 2005
    ..These results suggest that both ubiquitination and deubiquitination of Atg19p are required for its full function...
  11. ncbi request reprint Increased nuclear envelope permeability and Pep4p-dependent degradation of nucleoporins during hydrogen peroxide-induced cell death
    D Adam Mason
    Department of Biology, University of Rochester, NY 14627, USA
    FEMS Yeast Res 5:1237-51. 2005
    ..We conclude that increases in ROS and NPC permeability occur relatively early during H(2)O(2)-induced cell death. Later, Pep4p migrates out of vacuoles and degrades nucleoporins after the cells are effectively dead...
  12. pmc Targeting of Tsc13p to nucleus-vacuole junctions: a role for very-long-chain fatty acids in the biogenesis of microautophagic vesicles
    Erik Kvam
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Mol Biol Cell 16:3987-98. 2005
    ..These results indicate that Nvj1p retains and compartmentalizes Tsc13p at NV junctions and that VLCFAs contribute to the normal biogenesis of trilaminar PMN structures in yeast...
  13. ncbi request reprint Nvj1p is the outer-nuclear-membrane receptor for oxysterol-binding protein homolog Osh1p in Saccharomyces cerevisiae
    Erik Kvam
    Department of Biology, University of Rochester, 436 Hutchison Hall, NY 14627, USA
    J Cell Sci 117:4959-68. 2004
    ..We conclude the formation of nuclear PMN vesicles requires the overlapping activities of Osh1p and other Osh family members...
  14. pmc Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae
    Paul Roberts
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Mol Biol Cell 14:129-41. 2003
    ..These results introduce a novel mode of selective microautophagy that targets nonessential components of the yeast nucleus for degradation and recycling in the vacuole...
  15. pmc Binding dynamics of structural nucleoporins govern nuclear pore complex permeability and may mediate channel gating
    Nataliya Shulga
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Mol Cell Biol 23:534-42. 2003
    ..These results are consistent with the hypothesis that NPC gating occurs when molecular latches composed of FG repeats and structural nucleoporins dissociate...
  16. pmc Drosophila melanogaster importin alpha1 and alpha3 can replace importin alpha2 during spermatogenesis but not oogenesis
    D Adam Mason
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Genetics 161:157-70. 2002
    ..These rescue experiments suggest that male and female gametogenesis have distinct requirements for importin alpha2...
  17. pmc The Drosophila melanogaster importin alpha3 locus encodes an essential gene required for the development of both larval and adult tissues
    D Adam Mason
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Genetics 165:1943-58. 2003
    ..These results are consistent with the view that the importin alpha's have both overlapping and distinct functions and that their role in animal development involves the spatial and temporal control of their expression...
  18. ncbi request reprint Minimal nuclear pore complexes define FG repeat domains essential for transport
    Lisa A Strawn
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, 3120A MRBIII, 465 21st Avenue South, Nashville, TN 37232 8240, USA
    Nat Cell Biol 6:197-206. 2004
    ..Significantly, symmetric deletions caused mild reductions in Kap95-Kap60-mediated import rates, but virtually abolished Kap104 import. These results suggest the existence of multiple translocation pathways...

Research Grants8

  1. Molecular Screen for Lifespan Extension in Yeast (RMI)
    David Goldfarb; Fiscal Year: 2004
    ..Thus, yeast remains fertile ground for gene discovery, for the elucidation of conserved aging mechanisms, and for studies of functional senescence. ..
  2. High Through-Put Genomics Approach To Lifespan in Yeast
    David Goldfarb; Fiscal Year: 2006
    ..The resulting public data set is likely to identify new aging genes that could be of considerable importance to human aging. ..
  3. Yeast Lifespan Chemical Screening (RMI)
    David Goldfarb; Fiscal Year: 2005
    ..Promising compounds can be readily assessed using other lifespan assays in yeast, worms, and fruit flies. The assay results can be easily formatted in raw and processed formats to maximize their sharing and utility to others. ..
  4. Gating the nuclear pore complex translocon
    David Goldfarb; Fiscal Year: 2006
    ..Nuclear transport is a fundamental cell process that is intimately involved in gene expression and is relevant to issues of human disease and health. ..