Vadim N Gladyshev

Summary

Affiliation: University of Nebraska
Country: USA

Publications

  1. pmc The Plasmodium selenoproteome
    Alexey V Lobanov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Nucleic Acids Res 34:496-505. 2006
  2. pmc Nematode selenoproteome: the use of the selenocysteine insertion system to decode one codon in an animal genome?
    Kalin Taskov
    Department of Biochemistry, University of Nebraska Lincoln, NE 68588, USA
    Nucleic Acids Res 33:2227-38. 2005
  3. pmc SECIS elements in the coding regions of selenoprotein transcripts are functional in higher eukaryotes
    Heiko Mix
    Department of Biochemistry, University of Nebraska Beadle Center, Lincoln, NE 68588, USA
    Nucleic Acids Res 35:414-23. 2007
  4. pmc High content of proteins containing 21st and 22nd amino acids, selenocysteine and pyrrolysine, in a symbiotic deltaproteobacterium of gutless worm Olavius algarvensis
    Yan Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Nucleic Acids Res 35:4952-63. 2007
  5. pmc A structure-based approach for detection of thiol oxidoreductases and their catalytic redox-active cysteine residues
    Stefano M Marino
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, Nebraska, USA
    PLoS Comput Biol 5:e1000383. 2009
  6. pmc Selenium metabolism in Trypanosoma: characterization of selenoproteomes and identification of a Kinetoplastida-specific selenoprotein
    Alexey V Lobanov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Nucleic Acids Res 34:4012-24. 2006
  7. pmc Comparative genomic analyses of copper transporters and cuproproteomes reveal evolutionary dynamics of copper utilization and its link to oxygen
    Perry G Ridge
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, USA
    PLoS ONE 3:e1378. 2008
  8. pmc Selenocysteine insertion directed by the 3'-UTR SECIS element in Escherichia coli
    Dan Su
    Department of Biochemistry, University of Nebraska Lincoln Lincoln, NE 68588, USA
    Nucleic Acids Res 33:2486-92. 2005
  9. pmc Comparative genomic analyses of nickel, cobalt and vitamin B12 utilization
    Yan Zhang
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588 0664, USA
    BMC Genomics 10:78. 2009
  10. ncbi request reprint Identification of trace element-containing proteins in genomic databases
    Vadim N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Annu Rev Nutr 24:579-96. 2004

Research Grants

  1. Redox mechanism of cancer prevention by selenium
    VADIM GLADYSHEV; Fiscal Year: 2002
  2. 2006 Thiol-based Redox Regulation and Signaling Gordon Research Conference
    VADIM GLADYSHEV; Fiscal Year: 2006
  3. Redox Biology Center
    VADIM GLADYSHEV; Fiscal Year: 2007
  4. Redox Biology Center
    VADIM GLADYSHEV; Fiscal Year: 2008

Collaborators

Detail Information

Publications106 found, 100 shown here

  1. pmc The Plasmodium selenoproteome
    Alexey V Lobanov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Nucleic Acids Res 34:496-505. 2006
    ..Dependence of the plasmodial parasites on selenium suggests possible strategies for antimalarial drug development...
  2. pmc Nematode selenoproteome: the use of the selenocysteine insertion system to decode one codon in an animal genome?
    Kalin Taskov
    Department of Biochemistry, University of Nebraska Lincoln, NE 68588, USA
    Nucleic Acids Res 33:2227-38. 2005
    ....
  3. pmc SECIS elements in the coding regions of selenoprotein transcripts are functional in higher eukaryotes
    Heiko Mix
    Department of Biochemistry, University of Nebraska Beadle Center, Lincoln, NE 68588, USA
    Nucleic Acids Res 35:414-23. 2007
    ..Our data provide evidence that the location of the SECIS element in the untranslated region is not a functional necessity but rather is an evolutionary adaptation to enable a more efficient synthesis of selenoproteins...
  4. pmc High content of proteins containing 21st and 22nd amino acids, selenocysteine and pyrrolysine, in a symbiotic deltaproteobacterium of gutless worm Olavius algarvensis
    Yan Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Nucleic Acids Res 35:4952-63. 2007
    ..Anaerobic conditions and consistent selenium supply might be the factors that support the use of amino acids that extend the genetic code...
  5. pmc A structure-based approach for detection of thiol oxidoreductases and their catalytic redox-active cysteine residues
    Stefano M Marino
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, Nebraska, USA
    PLoS Comput Biol 5:e1000383. 2009
    ..This study provides insights into the structural properties of catalytic redox-active Cys and should further help to recognize thiol oxidoreductases in protein sequence and structure databases...
  6. pmc Selenium metabolism in Trypanosoma: characterization of selenoproteomes and identification of a Kinetoplastida-specific selenoprotein
    Alexey V Lobanov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Nucleic Acids Res 34:4012-24. 2006
    ..Overall, these data establish that Trypanosoma, Leishmania and likely other Kinetoplastida utilize and depend on the trace element selenium, and this dependence is due to occurrence of selenium in at least three selenoproteins...
  7. pmc Comparative genomic analyses of copper transporters and cuproproteomes reveal evolutionary dynamics of copper utilization and its link to oxygen
    Perry G Ridge
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, USA
    PLoS ONE 3:e1378. 2008
    ..Prokaryotic cuproproteomes were small and dominated by a single protein, cytochrome c oxidase. The data are consistent with the idea that proteins evolved to utilize copper following the oxygenation of the Earth...
  8. pmc Selenocysteine insertion directed by the 3'-UTR SECIS element in Escherichia coli
    Dan Su
    Department of Biochemistry, University of Nebraska Lincoln Lincoln, NE 68588, USA
    Nucleic Acids Res 33:2486-92. 2005
    ....
  9. pmc Comparative genomic analyses of nickel, cobalt and vitamin B12 utilization
    Yan Zhang
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588 0664, USA
    BMC Genomics 10:78. 2009
    ..Although a number of Ni and Co-dependent enzymes have been characterized, systematic evolutionary analyses of utilization of these metals are limited...
  10. ncbi request reprint Identification of trace element-containing proteins in genomic databases
    Vadim N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Annu Rev Nutr 24:579-96. 2004
    ..It also should be possible to search for entire sets of other trace element-associated proteins, such as metal-containing proteins, although methods for their identification are still in development...
  11. ncbi request reprint Evolution of selenocysteine-containing proteins: significance of identification and functional characterization of selenoproteins
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biofactors 14:87-92. 2001
    ..Further understanding of the evolution of selenoproteins as well as biological properties and biomedical applications of the trace element selenium requires identification and functional characterization of all mammalian selenoproteins...
  12. ncbi request reprint Identification and characterization of a new mammalian glutaredoxin (thioltransferase), Grx2
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 276:30374-80. 2001
    ..The Grx2 structural model suggested a common reaction mechanism for this class of proteins. The data provide the first example of a mitochondrial Grx and also indicate the occurrence of a second functional Grx in mammals...
  13. ncbi request reprint Thioredoxin and peptide methionine sulfoxide reductase: convergence of similar structure and function in distinct structural folds
    Vadim N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Proteins 46:149-52. 2002
    ..The data suggest that similar structures and functions of Trx and PMSR were acquired independently during evolution and point to a general strategy of identifying new redox regulatory proteins...
  14. ncbi request reprint Mammalian selenoprotein thioredoxin-glutathione reductase. Roles in disulfide bond formation and sperm maturation
    Dan Su
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 280:26491-8. 2005
    ..Together, TGR and GPx4 can serve as a novel disulfide bond formation system. Both enzymes contain a catalytic selenocysteine consistent with the role of selenium in male reproduction...
  15. ncbi request reprint Characterization of mammalian selenoproteomes
    Gregory V Kryukov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Science 300:1439-43. 2003
    ..The human selenoproteome consists of 25 selenoproteins...
  16. pmc Functional analysis of free methionine-R-sulfoxide reductase from Saccharomyces cerevisiae
    Dung Tien Le
    Department of Biochemistry and Redox Biology Center, University of Nebraska Lincoln, Lincoln, Nebraska 68588, USA
    J Biol Chem 284:4354-64. 2009
    ..The data show that fRMsr is the main enzyme responsible for the reduction of free Met-R-SO in S. cerevisiae...
  17. ncbi request reprint High-throughput identification of catalytic redox-active cysteine residues
    Dmitri E Fomenko
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Science 315:387-9. 2007
    ..Rapid accumulation of sequence information from genomic and metagenomic projects should allow detection of many additional oxidoreductase families as well as identification of redox-active Cys in these proteins...
  18. pmc Mammalian thioredoxin reductase 1: roles in redox homoeostasis and characterization of cellular targets
    Anton A Turanov
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588, U S A
    Biochem J 430:285-93. 2010
    ..Diminished hepatic TR1 function was associated with elevated Trx1 levels, but this protein was mostly in the oxidized state. Overall, this study provides evidence for the key role of the TR1-Trx1 pair in redox homoeostasis...
  19. ncbi request reprint SelT, SelW, SelH, and Rdx12: genomics and molecular insights into the functions of selenoproteins of a novel thioredoxin-like family
    Alexander Dikiy
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 46:6871-82. 2007
    ..Rdx12 was found to interact with glutathione peroxidase 1, whereas 14-3-3 protein was identified as one of the targets of mammalian SelW, suggesting a mechanism for redox regulation of the 14-3-3 family of proteins...
  20. pmc MsrB1 (methionine-R-sulfoxide reductase 1) knock-out mice: roles of MsrB1 in redox regulation and identification of a novel selenoprotein form
    Dmitri E Fomenko
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 284:5986-93. 2009
    ..Overall, this study established an important contribution of MsrB1 to the redox control in mouse liver and kidney and identified a novel form of this protein...
  21. ncbi request reprint Identity and functions of CxxC-derived motifs
    Dmitri E Fomenko
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 42:11214-25. 2003
    ..Each of the CxxC-derived motifs was also observed in natural selenium-containing proteins, in which selenocysteine was present in place of a catalytic cysteine...
  22. ncbi request reprint Reaction mechanism and regulation of mammalian thioredoxin/glutathione reductase
    Qi An Sun
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 44:14528-37. 2005
    ....
  23. pmc The microbial selenoproteome of the Sargasso Sea
    Yan Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Genome Biol 6:R37. 2005
    ..Herein, we characterized the selenoproteome of the largest microbial sequence dataset, the Sargasso Sea environmental genome project...
  24. pmc Analysis of methionine/selenomethionine oxidation and methionine sulfoxide reductase function using methionine-rich proteins and antibodies against their oxidized forms
    Dung Tien Le
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 47:6685-94. 2008
    ..These findings, reagents, assays, and approaches should facilitate research and applications in the area of Met sulfoxide reduction, oxidative stress, and aging...
  25. pmc Identification and characterization of a selenoprotein family containing a diselenide bond in a redox motif
    Valentina A Shchedrina
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 104:13919-24. 2007
    ..In a bacterial expression system, we directly demonstrated the formation of a diselenide bond between the two Sec, establishing it as the first diselenide bond found in a natural protein...
  26. pmc Regulation of selenoproteins and methionine sulfoxide reductases A and B1 by age, calorie restriction, and dietary selenium in mice
    Sergey V Novoselov
    Department of Biochemistry, University of Nebraska, Lincoln, NE, USA
    Antioxid Redox Signal 12:829-38. 2010
    ..Taken together, these findings better define dietary regulation of methionine sulfoxide reduction and selenoprotein expression in mice with regard to age, calorie restriction, dietary Se, and a combination of these factors...
  27. pmc Different catalytic mechanisms in mammalian selenocysteine- and cysteine-containing methionine-R-sulfoxide reductases
    Hwa Young Kim
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, USA
    PLoS Biol 3:e375. 2005
    ..The data also suggested that Sec- and Cys-containing oxidoreductases require distinct sets of active-site features that maximize their catalytic efficiencies and provide strategies for protein design with improved catalytic properties...
  28. ncbi request reprint Role of structural and functional elements of mouse methionine-S-sulfoxide reductase in its subcellular distribution
    Hwa Young Kim
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 44:8059-67. 2005
    ....
  29. pmc Evolutionary dynamics of eukaryotic selenoproteomes: large selenoproteomes may associate with aquatic life and small with terrestrial life
    Alexey V Lobanov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Genome Biol 8:R198. 2007
    ..Selenoproteins occur in both eukaryotes and prokaryotes, but the selenoprotein content of organisms (selenoproteome) is highly variable and some organisms do not utilize Sec at all...
  30. pmc X-ray fluorescence microscopy reveals the role of selenium in spermatogenesis
    Sebastian Kehr
    Redox Biology Center and Department of Biochemistry, University of Nebraska, Lincoln, 68588, USA
    J Mol Biol 389:808-18. 2009
    ..Overall, the use of XFM allowed visualization of tissue and cellular Se and provided important insights in the role of this and other trace elements in spermatogenesis...
  31. ncbi request reprint Selenoprotein H is a nucleolar thioredoxin-like protein with a unique expression pattern
    Sergey V Novoselov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 282:11960-8. 2007
    ..Overall, these data establish SelH as a novel nucleolar oxidoreductase and suggest that some functions in this compartment are regulated by redox and dependent on the trace element selenium...
  32. pmc Thiol peroxidases mediate specific genome-wide regulation of gene expression in response to hydrogen peroxide
    Dmitri E Fomenko
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 108:2729-34. 2011
    ..The data suggest that thiol peroxidases sense and transfer oxidative signals to the signaling proteins and regulate transcription, whereas a direct interaction between H(2)O(2) and other cellular proteins plays a secondary role...
  33. pmc Reduced reliance on the trace element selenium during evolution of mammals
    Alexey V Lobanov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Genome Biol 9:R62. 2008
    ....
  34. pmc Trends in selenium utilization in marine microbial world revealed through the analysis of the global ocean sampling (GOS) project
    Yan Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, USA
    PLoS Genet 4:e1000095. 2008
    ..Overall, our study provides insights into global trends in microbial selenium utilization in marine environments...
  35. ncbi request reprint A novel cysteine-rich domain of Sep15 mediates the interaction with UDP-glucose:glycoprotein glucosyltransferase
    Vyacheslav M Labunskyy
    Department of Biochemistry, University of Nebraska, Lincoln, 68588, USA
    J Biol Chem 280:37839-45. 2005
    ..These data indicate that the cysteine-rich domain of Sep15 exclusively mediates protein-protein interactions with GT...
  36. pmc Genetic code supports targeted insertion of two amino acids by one codon
    Anton A Turanov
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588, USA
    Science 323:259-61. 2009
    ..Thus, the genetic code supports the use of one codon to code for multiple amino acids...
  37. pmc CUG start codon generates thioredoxin/glutathione reductase isoforms in mouse testes
    Maxim V Gerashchenko
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 285:4595-602. 2010
    ..This study shows that CUG codon is used as an inefficient start codon to generate protein isoforms in mouse...
  38. pmc Characterization of surface-exposed reactive cysteine residues in Saccharomyces cerevisiae
    Stefano M Marino
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 49:7709-21. 2010
    ....
  39. ncbi request reprint Pyrrolysine and selenocysteine use dissimilar decoding strategies
    Yan Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 280:20740-51. 2005
    ..Thus, Sec and Pyl follow dissimilar decoding and evolutionary strategies...
  40. pmc Methionine sulfoxide reductase regulation of yeast lifespan reveals reactive oxygen species-dependent and -independent components of aging
    Ahmet Koc
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 101:7999-8004. 2004
    ..The data also suggest a model wherein factors implicated in aging (for example, ROS) may influence the lifespan yet not be the cause of aging...
  41. ncbi request reprint Alternative splicing involving the thioredoxin reductase module in mammals: a glutaredoxin-containing thioredoxin reductase 1
    Dan Su
    Department of Biochemistry, University of Nebraska Lincoln, Lincoln, Nebraska 68588, USA
    Biochemistry 43:12177-88. 2004
    ..Our data also suggest that various N-terminal extensions in mammalian TRs are often expressed in testes...
  42. ncbi request reprint Reaction mechanism, evolutionary analysis, and role of zinc in Drosophila methionine-R-sulfoxide reductase
    R Abhilash Kumar
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 277:37527-35. 2002
    ..A Drosophila MsrA gene was also cloned, and the recombinant enzyme was found to be metal-free and specific for methionine S-sulfoxide and to employ a similar sulfenic acid/disulfide mechanism...
  43. pmc Diversity of protein and mRNA forms of mammalian methionine sulfoxide reductase B1 due to intronization and protein processing
    Xinwen Liang
    Department of Biochemistry and Redox Biology Center, University of Nebraska Lincoln, Lincoln, Nebraska, United States of America
    PLoS ONE 5:e11497. 2010
    ..MsrB1 is a selenocysteine-containing cytosolic/nuclear Msr with high expression in liver and kidney...
  44. ncbi request reprint Is there a twenty third amino acid in the genetic code?
    Alexey V Lobanov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Trends Genet 22:357-60. 2006
    ..Our data suggest that the occurrence of additional amino acids that are widely distributed and genetically encoded is unlikely...
  45. pmc Dynamic evolution of selenocysteine utilization in bacteria: a balance between selenoprotein loss and evolution of selenocysteine from redox active cysteine residues
    Yan Zhang
    Department of Biochemistry, University of Nebraska, 1901 Vine Street, Lincoln, NE 68588 0664, USA
    Genome Biol 7:R94. 2006
    ..It occurs in oxidoreductase active sites and often is catalytically superior to cysteine (Cys). However, Sec is used very selectively in proteins and organisms. The wide distribution of Sec and its restricted use have not been explained...
  46. pmc Methionine sulfoxide reduction in mammals: characterization of methionine-R-sulfoxide reductases
    Hwa Young Kim
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Mol Biol Cell 15:1055-64. 2004
    ..The data suggested that different cellular compartments in mammals maintain a system for repair of oxidized methionine residues and that this function is tuned in enzyme- and stereo-specific manner...
  47. pmc In silico identification of genes involved in selenium metabolism: evidence for a third selenium utilization trait
    Yan Zhang
    Redox Biology Center and Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    BMC Genomics 9:251. 2008
    ..Selenophosphate synthetase (SelD) is required for both utilization traits. However, previous research also revealed SelDs in two organisms lacking Sec and SeU, suggesting a possible additional use of Se that is dependent on SelD...
  48. pmc Structure-function relations, physiological roles, and evolution of mammalian ER-resident selenoproteins
    Valentina A Shchedrina
    Redox Biology Center and Department of Biochemistry, University of Nebraska, Lincoln, NE, USA
    Antioxid Redox Signal 12:839-49. 2010
    ..This review discusses evolution and structure-function relations of the ER-resident selenoproteins and summarizes recent findings on these proteins, which reveal the emerging important role of selenium and selenoproteins in ER function...
  49. ncbi request reprint Characterization of mouse endoplasmic reticulum methionine-R-sulfoxide reductase
    Hwa Young Kim
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Biochem Biophys Res Commun 320:1277-83. 2004
    ..The data suggest that, in mice, MsrB3 is largely or exclusively an ER-resident protein, and that the reduction of methionine-R-sulfoxides in different cellular compartments is provided by individual MsrB isozymes...
  50. pmc Characterization of methionine oxidation and methionine sulfoxide reduction using methionine-rich cysteine-free proteins
    Xinwen Liang
    Department of Biochemistry, University of Nebraska, Lincoln, 68588, USA
    BMC Biochem 13:21. 2012
    ..Conventional methods of assaying MetO formation and reduction rely on chromatographic or mass spectrometry procedures, but the use of Met-rich proteins (MRPs) may offer a more streamlined alternative...
  51. ncbi request reprint A conserved cis-proline precludes metal binding by the active site thiolates in members of the thioredoxin family of proteins
    Dan Su
    Department of Biochemistry, Univeristy of Nebraska Lincoln, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 46:6903-10. 2007
    ..Taken together, our data suggest that an important function of Pro75 in human thioredoxin, and likely other members of this superfamily, is to prevent metal binding by the reactive thiolate-based active site...
  52. ncbi request reprint Characterization of alternative cytosolic forms and cellular targets of mouse mitochondrial thioredoxin reductase
    Anton A Turanov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 281:22953-63. 2006
    ..Overall, these data establish that the function of TR3 is not limited to its role in Trx2 reduction...
  53. pmc Identification and characterization of Fep15, a new selenocysteine-containing member of the Sep15 protein family
    Sergey V Novoselov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Biochem J 394:575-9. 2006
    ..Phylogenetic analyses of Sep15 family members suggest that Fep15 evolved by gene duplication...
  54. pmc The prokaryotic selenoproteome
    Gregory V Kryukov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    EMBO Rep 5:538-43. 2004
    ..Characterization of selenoproteomes allows interpretation of other UGA codons in completed genomes of prokaryotes as terminators, addressing the UGA dual-function problem...
  55. pmc Selenoproteins and selenocysteine insertion system in the model plant cell system, Chlamydomonas reinhardtii
    Sergey V Novoselov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    EMBO J 21:3681-93. 2002
    ....
  56. ncbi request reprint An algorithm for identification of bacterial selenocysteine insertion sequence elements and selenoprotein genes
    Yan Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Bioinformatics 21:2580-9. 2005
    ..Whereas SECIS elements in Escherichia coli are well characterized, a bacterial SECIS consensus structure is lacking...
  57. pmc Functional diversity of cysteine residues in proteins and unique features of catalytic redox-active cysteines in thiol oxidoreductases
    Dmitri E Fomenko
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588 0664, USA
    Mol Cells 26:228-35. 2008
    ....
  58. pmc Alternative first exon splicing regulates subcellular distribution of methionine sulfoxide reductases
    Hwa Young Kim
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    BMC Mol Biol 7:11. 2006
    ..In mammals, a single MsrA gene is known, however, its product is present in cytosol, nucleus, and mitochondria. In contrast, three mammalian MsrB genes have been identified whose products are located in different cellular compartments...
  59. pmc Selenocysteine in thiol/disulfide-like exchange reactions
    Robert J Hondal
    Given Laboratory, Department of Biochemistry, University of Vermont, Burlington, VT 05405, USA
    Antioxid Redox Signal 18:1675-89. 2013
    ..Sec differs from cysteine (Cys) by only one atom (selenium versus sulfur), yet this switch dramatically influences important aspects of enzyme reactivity...
  60. pmc Mammals reduce methionine-S-sulfoxide with MsrA and are unable to reduce methionine-R-sulfoxide, and this function can be restored with a yeast reductase
    Byung Cheon Lee
    Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 283:28361-9. 2008
    ....
  61. pmc Compartmentalization and regulation of mitochondrial function by methionine sulfoxide reductases in yeast
    Alaattin Kaya
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 49:8618-25. 2010
    ..These results show that oxidative stress contributes to mitochondrial dysfunction through oxidation of methionine residues in proteins located in different cellular compartments...
  62. ncbi request reprint New mammalian selenocysteine-containing proteins identified with an algorithm that searches for selenocysteine insertion sequence elements
    G V Kryukov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 274:33888-97. 1999
    ..SelR homologs were present in every organism characterized by complete genome sequencing. The data suggest applicability of SECISearch for identification of new selenoprotein genes in nucleotide data bases...
  63. pmc Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging
    Valentina A Shchedrina
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588, USA
    Mech Ageing Dev 130:429-43. 2009
    ..Thus, MsrA and MsrB, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies...
  64. pmc Selenoprotein R is a zinc-containing stereo-specific methionine sulfoxide reductase
    Gregory V Kryukov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 99:4245-50. 2002
    ....
  65. pmc CxxS: fold-independent redox motif revealed by genome-wide searches for thiol/disulfide oxidoreductase function
    Dmitri E Fomenko
    Department of Biochemistry, University of Nebraska Lincoln, 68588 0664, USA
    Protein Sci 11:2285-96. 2002
    ..The data also indicate a general approach of genome-wide identification of redox proteins by searching for simple conserved motifs within secondary structure patterns...
  66. ncbi request reprint Comparative analysis of selenocysteine machinery and selenoproteome gene expression in mouse brain identifies neurons as key functional sites of selenium in mammals
    Yan Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 283:2427-38. 2008
    ..Overall, this study suggests that the main functions of selenium in mammals are confined to certain neurons in the brain...
  67. pmc Functions and evolution of selenoprotein methionine sulfoxide reductases
    Byung Cheon Lee
    Redox Biology Center and Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biochim Biophys Acta 1790:1471-7. 2009
    ..Msrs have roles in protecting cellular proteins from oxidative stress and through this function they may regulate lifespan in several model organisms...
  68. ncbi request reprint Selenoprotein deficiency and high levels of selenium compounds can effectively inhibit hepatocarcinogenesis in transgenic mice
    Sergey V Novoselov
    Department of Biochemistry, University of Nebraska, N 151 Beadle Center, 1901 Vine Street, Lincoln, NE 68588, USA
    Oncogene 24:8003-11. 2005
    ..Since dietary Se may have opposing effects on cancer, it is important to identify the subjects who will benefit from Se supplementation as well as those who will not...
  69. pmc Structural analysis of cysteine S-nitrosylation: a modified acid-based motif and the emerging role of trans-nitrosylation
    Stefano M Marino
    Department of Biochemistry and Redox Biology Center, University of Nebraska Lincoln, Lincoln, NE 68588, USA
    J Mol Biol 395:844-59. 2010
    ..For proteins lacking the revised motif, we discuss alternative mechanisms including a potential role of nitrosoglutathione as a trans-acting agent...
  70. pmc A highly efficient form of the selenocysteine insertion sequence element in protozoan parasites and its use in mammalian cells
    Sergey V Novoselov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Proc Natl Acad Sci U S A 104:7857-62. 2007
    ..It contains an SBP2 gene and the most efficient tested SECIS element: an AUGA mutant of the GGGA-type Toxoplasma SelT structure...
  71. ncbi request reprint Mammalian selenoprotein gene signature: identification and functional analysis of selenoprotein genes using bioinformatics methods
    Gregory V Kryukov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Methods Enzymol 347:84-100. 2002
  72. ncbi request reprint Redox regulation of cell signaling by selenocysteine in mammalian thioredoxin reductases
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 274:24522-30. 1999
    ..These observations identify the carboxyl-terminal selenocysteine of TR1 as a cellular redox sensor and support an essential role for mammalian TR isozymes in redox-regulated cell signaling...
  73. ncbi request reprint Selenium metabolism in zebrafish: multiplicity of selenoprotein genes and expression of a protein containing 17 selenocysteine residues
    G V Kryukov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Genes Cells 5:1049-60. 2000
    ..Selenium is present in vertebrate proteins in the form of selenocysteine (Sec), the 21st natural amino acid in protein which is encoded by UGA...
  74. pmc Non-animal origin of animal thioredoxin reductases: implications for selenocysteine evolution and evolution of protein function through carboxy-terminal extensions
    Sergey V Novoselov
    Department of Biochemistry, University of Nebraska, Lincoln 68588 0664, USA
    Protein Sci 12:372-8. 2003
    ..Our data implicate carboxy-terminal extension of proteins as a general mechanism of evolution of new protein function...
  75. ncbi request reprint Selenocysteine-containing thioredoxin reductase in C. elegans
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochem Biophys Res Commun 259:244-9. 1999
    ..The data show that TR-Se is the major naturally occurring selenoprotein in C. elegans, and suggest an important role for selenium and the thioredoxin system in this organism...
  76. ncbi request reprint Selenocysteine-containing proteins in mammals
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    J Biomed Sci 6:151-60. 1999
    ..This review describes known mammalian selenoproteins and discusses recent developments and future directions in the selenium field...
  77. ncbi request reprint A new human selenium-containing protein. Purification, characterization, and cDNA sequence
    V N Gladyshev
    Section on the Molecular Biology of Selenium, Basic Research Laboratory, NCI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 273:8910-5. 1998
    ..The discovery of a new human selenoprotein provides an additional example of the role of selenium in mammalian systems...
  78. ncbi request reprint Association between the 15-kDa selenoprotein and UDP-glucose:glycoprotein glucosyltransferase in the endoplasmic reticulum of mammalian cells
    K V Korotkov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 276:15330-6. 2001
    ..This report provides the first example of the ER-resident selenoprotein and suggests a possible role of the trace element selenium in the quality control of protein folding...
  79. pmc Levels of major selenoproteins in T cells decrease during HIV infection and low molecular mass selenium compounds increase
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 96:835-9. 1999
    ..In contrast to selenoproteins, low molecular mass [75Se]compounds accumulated during HIV infection and migrated as a diffuse band near the front of SDS/PAGE gels...
  80. ncbi request reprint Contrasting patterns of regulation of the antioxidant selenoproteins, thioredoxin reductase, and glutathione peroxidase, in cancer cells
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, 68588, USA
    Biochem Biophys Res Commun 251:488-93. 1998
    ..The data suggest that additional studies on selenoprotein regulation in different cancers are required to evaluate future implementation of selenium as a dietary supplement in individuals at risk for developing certain cancers...
  81. ncbi request reprint Heterogeneity within animal thioredoxin reductases. Evidence for alternative first exon splicing
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 276:3106-14. 2001
    ..Multiple transcription start sites within TR genes may be relevant to complex regulation of expression and/or organelle- and cell type-specific location of animal thioredoxin reductases...
  82. pmc Eukaryotic selenoproteins and selenoproteomes
    Alexey V Lobanov
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588, USA
    Biochim Biophys Acta 1790:1424-8. 2009
    ..We also discuss selenoprotein functions and evolutionary trends in the use of these proteins in eukaryotes. Functional analysis of selenoproteins is critical for better understanding of the role of selenium in human health and disease...
  83. ncbi request reprint Genomics perspective on disulfide bond formation
    Dmitri E Fomenko
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    Antioxid Redox Signal 5:397-402. 2003
    ..Emerging evidence suggests that these ER functions might be assisted by mammalian selenocysteine-containing oxidoreductases Sep15 and SelM...
  84. pmc Molybdoproteomes and evolution of molybdenum utilization
    Yan Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Mol Biol 379:881-99. 2008
    ..Overall, our data provide new insights into Mo utilization and show its wide occurrence, yet limited use of this metal in individual organisms in all three domains of life...
  85. pmc Mammalian selenoprotein in which selenocysteine (Sec) incorporation is supported by a new form of Sec insertion sequence element
    Konstantin V Korotkov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Mol Cell Biol 22:1402-11. 2002
    ..SelM is expressed in a variety of tissues, with increased levels in the brain. It is localized to the perinuclear structures, and its N-terminal signal peptide is necessary for protein translocation...
  86. pmc Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systems
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 98:3673-8. 2001
    ....
  87. ncbi request reprint Redox regulation of cell signaling by thioredoxin reductases
    Qi An Sun
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Methods Enzymol 347:451-61. 2002
  88. pmc Semisynthesis and characterization of mammalian thioredoxin reductase
    Brian Eckenroth
    Department of Biochemistry, University of Vermont, 89 Beaumont Avenue, Given Laboratory, Room B413, Burlington, Vermont 05405, USA
    Biochemistry 45:5158-70. 2006
    ..This noncovalent peptide-enzyme complex has 350-500-fold lower activity than the semisynthetic enzyme produced by peptide ligation...
  89. pmc Selenoproteinless animals: selenophosphate synthetase SPS1 functions in a pathway unrelated to selenocysteine biosynthesis
    Alexey V Lobanov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Protein Sci 17:176-82. 2008
    ..Since SPS1 evolved from a protein that utilizes selenium for Sec biosynthesis, an attractive possibility is that SPS1 may define a new pathway of selenium utilization in animals...
  90. pmc Catalytic advantages provided by selenocysteine in methionine-S-sulfoxide reductases
    Hwa Young Kim
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 45:13697-704. 2006
    ..Overall, this study demonstrates that sporadically evolved Sec-containing forms of methionine sulfoxide reductases reflect catalytic advantages provided by Sec in these and likely other thiol-dependent oxidoreductases...
  91. ncbi request reprint Analysis of selenocysteine-containing proteins
    V N Gladyshev
    University of Nebraska, Lincoln, Nebraska, USA
    Curr Protoc Protein Sci . 2001
    ..This unit provides an overview of known selenocysteine-containing proteins, examples of targeted incorporation of selenium into proteins, and methods specific for selenoprotein identification and characterization...
  92. pmc Evolution of selenium utilization traits
    Hector Romero
    Laboratorio de Organización y Evolución del Genoma, Dpto de Biología Celular y Molecular, Instituto de Biologia, Facultad de Ciencias, Igua 4225, Montevideo, CP 11400, Uruguay
    Genome Biol 6:R66. 2005
    ..Selenium is also found in the form of 2-selenouridine, a modified base present in the wobble position of certain tRNAs, whose synthesis is catalyzed by YbbB using selenophosphate as a precursor...
  93. ncbi request reprint Evidence for direct roles of two additional factors, SECp43 and soluble liver antigen, in the selenoprotein synthesis machinery
    Xue Ming Xu
    Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:41568-75. 2005
    ..The data also reveal a role of SECp43 in regulation of selenoprotein expression by affecting the synthesis of Um34 on tRNA([Ser]Sec) and the intracellular location of SLA...
  94. ncbi request reprint NMR structures of the selenoproteins Sep15 and SelM reveal redox activity of a new thioredoxin-like family
    Andrew D Ferguson
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 281:3536-43. 2006
    ..A physiological role for Sep15 and SelM as thiol-disulfide oxidoreductases and their contribution to the quality control pathways of the endoplasmic reticulum are discussed...
  95. pmc Biosynthesis of selenocysteine on its tRNA in eukaryotes
    Xue Ming Xu
    Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Biol 5:e4. 2007
    ..Conservation of the overall pathway of Sec biosynthesis suggests that this pathway is also active in other eukaryotes and archaea that synthesize selenoproteins...
  96. ncbi request reprint Solution structure of selenoprotein W and NMR analysis of its interaction with 14-3-3 proteins
    Finn L Aachmann
    Department of Biotechnology, Norwegian University of Science and Technology, N 7491 Trondheim, Norway
    J Biol Chem 282:37036-44. 2007
    ..In the present work, with the aid of NMR spectroscopy, we demonstrated specificity of this interaction and identified mobile loops in SelW as interacting surfaces. This finding suggests that 14-3-3 are redox-regulated proteins...
  97. pmc The Chlamydomonas genome reveals the evolution of key animal and plant functions
    Sabeeha S Merchant
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Science 318:245-50. 2007
    ....
  98. ncbi request reprint Structure and catalytic mechanism of eukaryotic selenocysteine synthase
    Oleg M Ganichkin
    Max Planck Institut fur biophysikalische Chemie, Zelluläre Biochemie Makromolekulare Röntgenkristallographie, Am Fassberg 11, D 37077 Gottingen, Germany
    J Biol Chem 283:5849-65. 2008
    ..Based on these results and on the activity profiles of mechanism-based inhibitors, we offer a detailed reaction mechanism for the enzyme...
  99. pmc Platyhelminth mitochondrial and cytosolic redox homeostasis is controlled by a single thioredoxin glutathione reductase and dependent on selenium and glutathione
    Mariana Bonilla
    Catedra de Inmunologia, Facultad de Quimica Facultad de Ciencias, Instituto de Higiene, Universidad de la Republica, Piso 2, Montevideo, Uruguay
    J Biol Chem 283:17898-907. 2008
    ..These studies establish the selenium- and glutathione-dependent regulation of cytosolic and mitochondrial redox homeostasis through a single TGR enzyme in platyhelminths...
  100. ncbi request reprint Specific excision of the selenocysteine tRNA[Ser]Sec (Trsp) gene in mouse liver demonstrates an essential role of selenoproteins in liver function
    Bradley A Carlson
    Section on the Molecular Biology of Selenium, Basic Research Laboratory, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland, 20892, USA
    J Biol Chem 279:8011-7. 2004
    ..Death appeared to be due to severe hepatocellular degeneration and necrosis with concomitant necrosis of peritoneal and retroperitoneal fat. These studies revealed an essential role of selenoproteins in liver function...
  101. pmc How selenium has altered our understanding of the genetic code
    Dolph L Hatfield
    Molecular Biology of Selenium Section, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 22:3565-76. 2002

Research Grants4

  1. Redox mechanism of cancer prevention by selenium
    VADIM GLADYSHEV; Fiscal Year: 2002
    ..These approaches should yield insight into the mechanism of the anticancer effect of selenium and may lead to the identification of human cancers most susceptible to selenium chemoprevention. ..
  2. 2006 Thiol-based Redox Regulation and Signaling Gordon Research Conference
    VADIM GLADYSHEV; Fiscal Year: 2006
    ..A diverse mix of established and emerging leaders in the field, and of junior and senior investigators will be included in the program. [unreadable] [unreadable] [unreadable]..
  3. Redox Biology Center
    VADIM GLADYSHEV; Fiscal Year: 2007
    ..Elucidation of redox-linked mechanisms in diseases ranging from cataracts to cancers and neurodegeneration by RBC members hold promise for developing therapeutic strategies for intervention. ..
  4. Redox Biology Center
    VADIM GLADYSHEV; Fiscal Year: 2008
    ..Elucidation of redox-linked mechanisms in diseases ranging from cataracts to cancers and neurodegeneration by RBC members hold promise for developing therapeutic strategies for intervention. ..