V N Gladyshev

Summary

Affiliation: University of Nebraska
Country: USA

Publications

  1. ncbi request reprint Selenocysteine-containing thioredoxin reductase in C. elegans
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochem Biophys Res Commun 259:244-9. 1999
  2. ncbi request reprint Contrasting patterns of regulation of the antioxidant selenoproteins, thioredoxin reductase, and glutathione peroxidase, in cancer cells
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, 68588, USA
    Biochem Biophys Res Commun 251:488-93. 1998
  3. pmc Levels of major selenoproteins in T cells decrease during HIV infection and low molecular mass selenium compounds increase
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 96:835-9. 1999
  4. ncbi request reprint Selenocysteine-containing proteins in mammals
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    J Biomed Sci 6:151-60. 1999
  5. ncbi request reprint Identification and characterization of a new mammalian glutaredoxin (thioltransferase), Grx2
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 276:30374-80. 2001
  6. ncbi request reprint Heterogeneity within animal thioredoxin reductases. Evidence for alternative first exon splicing
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 276:3106-14. 2001
  7. pmc Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systems
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 98:3673-8. 2001
  8. ncbi request reprint Selenium metabolism in zebrafish: multiplicity of selenoprotein genes and expression of a protein containing 17 selenocysteine residues
    G V Kryukov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Genes Cells 5:1049-60. 2000
  9. ncbi request reprint Redox regulation of cell signaling by selenocysteine in mammalian thioredoxin reductases
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 274:24522-30. 1999
  10. ncbi request reprint Evolution of selenocysteine-containing proteins: significance of identification and functional characterization of selenoproteins
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biofactors 14:87-92. 2001

Collaborators

  • K T Jeang
  • Y Zhou
  • M F Lou
  • Y Wu
  • J C Wootton
  • Y Hu
  • Q A Sun
  • D L Hatfield
  • G V Kryukov
  • K V Korotkov
  • E Kumaraswamy
  • B J Lee
  • F J Martin-Romero
  • B A Carlson
  • F Zappacosta
  • J C Boyington
  • V M Factor
  • A V Lobanov
  • P J Wirth
  • L Kirnarsky
  • S Sherman
  • S Y Kwon
  • S Kozyavkin
  • M E Moustafa
  • A M Diamond
  • M J Berry
  • A Malykh
  • V M Kryukov
  • S V Khangulov
  • T C Stadtman
  • P D Sun

Detail Information

Publications20

  1. ncbi request reprint Selenocysteine-containing thioredoxin reductase in C. elegans
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochem Biophys Res Commun 259:244-9. 1999
    ..The data show that TR-Se is the major naturally occurring selenoprotein in C. elegans, and suggest an important role for selenium and the thioredoxin system in this organism...
  2. ncbi request reprint Contrasting patterns of regulation of the antioxidant selenoproteins, thioredoxin reductase, and glutathione peroxidase, in cancer cells
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, 68588, USA
    Biochem Biophys Res Commun 251:488-93. 1998
    ..The data suggest that additional studies on selenoprotein regulation in different cancers are required to evaluate future implementation of selenium as a dietary supplement in individuals at risk for developing certain cancers...
  3. pmc Levels of major selenoproteins in T cells decrease during HIV infection and low molecular mass selenium compounds increase
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 96:835-9. 1999
    ..In contrast to selenoproteins, low molecular mass [75Se]compounds accumulated during HIV infection and migrated as a diffuse band near the front of SDS/PAGE gels...
  4. ncbi request reprint Selenocysteine-containing proteins in mammals
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    J Biomed Sci 6:151-60. 1999
    ..This review describes known mammalian selenoproteins and discusses recent developments and future directions in the selenium field...
  5. ncbi request reprint Identification and characterization of a new mammalian glutaredoxin (thioltransferase), Grx2
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 276:30374-80. 2001
    ..The Grx2 structural model suggested a common reaction mechanism for this class of proteins. The data provide the first example of a mitochondrial Grx and also indicate the occurrence of a second functional Grx in mammals...
  6. ncbi request reprint Heterogeneity within animal thioredoxin reductases. Evidence for alternative first exon splicing
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 276:3106-14. 2001
    ..Multiple transcription start sites within TR genes may be relevant to complex regulation of expression and/or organelle- and cell type-specific location of animal thioredoxin reductases...
  7. pmc Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systems
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 98:3673-8. 2001
    ....
  8. ncbi request reprint Selenium metabolism in zebrafish: multiplicity of selenoprotein genes and expression of a protein containing 17 selenocysteine residues
    G V Kryukov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Genes Cells 5:1049-60. 2000
    ..Selenium is present in vertebrate proteins in the form of selenocysteine (Sec), the 21st natural amino acid in protein which is encoded by UGA...
  9. ncbi request reprint Redox regulation of cell signaling by selenocysteine in mammalian thioredoxin reductases
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 274:24522-30. 1999
    ..These observations identify the carboxyl-terminal selenocysteine of TR1 as a cellular redox sensor and support an essential role for mammalian TR isozymes in redox-regulated cell signaling...
  10. ncbi request reprint Evolution of selenocysteine-containing proteins: significance of identification and functional characterization of selenoproteins
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biofactors 14:87-92. 2001
    ..Further understanding of the evolution of selenoproteins as well as biological properties and biomedical applications of the trace element selenium requires identification and functional characterization of all mammalian selenoproteins...
  11. ncbi request reprint New mammalian selenocysteine-containing proteins identified with an algorithm that searches for selenocysteine insertion sequence elements
    G V Kryukov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 274:33888-97. 1999
    ..SelR homologs were present in every organism characterized by complete genome sequencing. The data suggest applicability of SECISearch for identification of new selenoprotein genes in nucleotide data bases...
  12. ncbi request reprint Association between the 15-kDa selenoprotein and UDP-glucose:glycoprotein glucosyltransferase in the endoplasmic reticulum of mammalian cells
    K V Korotkov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 276:15330-6. 2001
    ..This report provides the first example of the ER-resident selenoprotein and suggests a possible role of the trace element selenium in the quality control of protein folding...
  13. ncbi request reprint Analysis of selenocysteine-containing proteins
    V N Gladyshev
    University of Nebraska, Lincoln, Nebraska, USA
    Curr Protoc Protein Sci . 2001
    ..This unit provides an overview of known selenocysteine-containing proteins, examples of targeted incorporation of selenium into proteins, and methods specific for selenoprotein identification and characterization...
  14. ncbi request reprint Selenium metabolism in Drosophila: selenoproteins, selenoprotein mRNA expression, fertility, and mortality
    F J Martin-Romero
    Section on the Molecular Biology of Selenium, Basic Research Laboratory, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:29798-804. 2001
    ..The data provide evidence that dietary selenium deficiency shortens, while supplementation of the diet with selenium normalizes the Drosophila life span by a process that may involve the newly identified selenoproteins...
  15. ncbi request reprint Structure-expression relationships of the 15-kDa selenoprotein gene. Possible role of the protein in cancer etiology
    E Kumaraswamy
    Section on the Molecular Biology of Selenium, Basic Research Laboratory, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 275:35540-7. 2000
    ....
  16. ncbi request reprint A new human selenium-containing protein. Purification, characterization, and cDNA sequence
    V N Gladyshev
    Section on the Molecular Biology of Selenium, Basic Research Laboratory, NCI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 273:8910-5. 1998
    ..The discovery of a new human selenoprotein provides an additional example of the role of selenium in mammalian systems...
  17. ncbi request reprint Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster
    J C Boyington
    Laboratory of Molecular Structure, National Institute of Allergy and Infectious Diseases, National Institutes of Health NIH, Rockville, MD 20852, USA
    Science 275:1305-8. 1997
    ..These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer...
  18. ncbi request reprint Characterization of crystalline formate dehydrogenase H from Escherichia coli. Stabilization, EPR spectroscopy, and preliminary crystallographic analysis
    V N Gladyshev
    Laboratory of Biochemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 271:8095-100. 1996
    ..These data provide the first example of crystallization of a substrate-reduced form of a Se- and Mo-containing enzyme...
  19. ncbi request reprint Distribution and functional consequences of nucleotide polymorphisms in the 3'-untranslated region of the human Sep15 gene
    Y J Hu
    Department of Human Nutrition and Dietetics, University of Illinois at Chicago, 60612, USA
    Cancer Res 61:2307-10. 2001
    ..This, together with genetic data indicating loss of heterozygosity at the Sep15 locus in certain human tumor types, suggests that Sep15 may be involved in cancer development, risk, or both...
  20. pmc Selenocysteine, identified as the penultimate C-terminal residue in human T-cell thioredoxin reductase, corresponds to TGA in the human placental gene
    V N Gladyshev
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 93:6146-51. 1996
    ..e., thioredoxin-thioredoxin reductase), in addition to the well-known glutathione peroxidase enzyme system...