Mark T Gladwin

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Doppler-defined pulmonary hypertension in medical intensive care unit patients: Retrospective investigation of risk factors and impact on mortality
    Jason A Stamm
    Department of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Pulm Circ 1:95-102. 2011
  2. pmc Elevated N-terminal pro-brain natriuretic peptide is associated with mortality in tobacco smokers independent of airflow obstruction
    Jason A Stamm
    Division of Pulmonary and Critical Care Medicine, Geisinger Medical Center, Danville, Pennsylvania, United States of America
    PLoS ONE 6:e27416. 2011
  3. pmc Expression of regulatory platelet microRNAs in patients with sickle cell disease
    Shilpa Jain
    Division of Pediatric Hematology Oncology, Children s Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    PLoS ONE 8:e60932. 2013
  4. pmc The relationship between the severity of hemolysis, clinical manifestations and risk of death in 415 patients with sickle cell anemia in the US and Europe
    Mehdi Nouraie
    Howard University, Washington, USA
    Haematologica 98:464-72. 2013
  5. pmc Pulmonary complications of sickle cell disease
    Andrew C Miller
    Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh Medical Center, 3459 Fifth Avenue, Pittsburgh, PA 15213, USA
    Am J Respir Crit Care Med 185:1154-65. 2012
  6. pmc Cardiovascular abnormalities in sickle cell disease
    Mark T Gladwin
    Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh, 3459 Fifth Avenue, Montefiore Hospital, Pittsburgh, PA 15213, USA
    J Am Coll Cardiol 59:1123-33. 2012
  7. pmc Hemolysis and cell-free hemoglobin drive an intrinsic mechanism for human disease
    Mark T Gladwin
    Vascular Medicine Institute and Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Clin Invest 122:1205-8. 2012
  8. ncbi request reprint Storage lesion in banked blood due to hemolysis-dependent disruption of nitric oxide homeostasis
    Mark T Gladwin
    Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Curr Opin Hematol 16:515-23. 2009
  9. doi request reprint Prevalence, risk factors and mortality of pulmonary hypertension defined by right heart catheterization in patients with sickle cell disease
    Mark T Gladwin
    Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA, USA and Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, USA
    Expert Rev Hematol 4:593-6. 2011
  10. doi request reprint The new chemical biology of nitrite reactions with hemoglobin: R-state catalysis, oxidative denitrosylation, and nitrite reductase/anhydrase
    Mark T Gladwin
    Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Acc Chem Res 42:157-67. 2009

Detail Information

Publications102 found, 100 shown here

  1. pmc Doppler-defined pulmonary hypertension in medical intensive care unit patients: Retrospective investigation of risk factors and impact on mortality
    Jason A Stamm
    Department of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Pulm Circ 1:95-102. 2011
    ..Prospective studies are needed to define the prevalence of pulmonary venous hypertension versus pulmonary arterial hypertension, and the clinical consequences of each, in a general medical intensive care unit population...
  2. pmc Elevated N-terminal pro-brain natriuretic peptide is associated with mortality in tobacco smokers independent of airflow obstruction
    Jason A Stamm
    Division of Pulmonary and Critical Care Medicine, Geisinger Medical Center, Danville, Pennsylvania, United States of America
    PLoS ONE 6:e27416. 2011
    ..We hypothesized that NT-proBNP would be an independent prognostic marker in a cohort of well-characterized tobacco smokers without known cardiovascular disease...
  3. pmc Expression of regulatory platelet microRNAs in patients with sickle cell disease
    Shilpa Jain
    Division of Pediatric Hematology Oncology, Children s Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    PLoS ONE 8:e60932. 2013
    ..This is the first study to determine whether platelets from SCD exhibit an altered miRNA expression profile...
  4. pmc The relationship between the severity of hemolysis, clinical manifestations and risk of death in 415 patients with sickle cell anemia in the US and Europe
    Mehdi Nouraie
    Howard University, Washington, USA
    Haematologica 98:464-72. 2013
    ..These results confirm associations between hemolytic rate and pulse pressure, oxygen saturation, increases in Doppler-estimated pulmonary systolic pressures and mortality (Clinicaltrials.gov identifier: NCT00492531)...
  5. pmc Pulmonary complications of sickle cell disease
    Andrew C Miller
    Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh Medical Center, 3459 Fifth Avenue, Pittsburgh, PA 15213, USA
    Am J Respir Crit Care Med 185:1154-65. 2012
    ..Clinicians should be vigilant in screening and treating such comorbidities to improve patient outcomes...
  6. pmc Cardiovascular abnormalities in sickle cell disease
    Mark T Gladwin
    Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh, 3459 Fifth Avenue, Montefiore Hospital, Pittsburgh, PA 15213, USA
    J Am Coll Cardiol 59:1123-33. 2012
    ..Sudden death is an increasingly recognized problem, and further cardiac investigations are necessary to recognize and treat high-risk patients...
  7. pmc Hemolysis and cell-free hemoglobin drive an intrinsic mechanism for human disease
    Mark T Gladwin
    Vascular Medicine Institute and Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Clin Invest 122:1205-8. 2012
    ....
  8. ncbi request reprint Storage lesion in banked blood due to hemolysis-dependent disruption of nitric oxide homeostasis
    Mark T Gladwin
    Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Curr Opin Hematol 16:515-23. 2009
    ..However, the degree of intravascular hemolysis and microparticle formation in humans post-transfusion due to both storage and physiological shear has not been well studied...
  9. doi request reprint Prevalence, risk factors and mortality of pulmonary hypertension defined by right heart catheterization in patients with sickle cell disease
    Mark T Gladwin
    Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA, USA and Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, USA
    Expert Rev Hematol 4:593-6. 2011
    ..The presence of pulmonary hypertension diagnosed by echocardiography or right heart catheterization indicates a significant and independent risk factor for prospective mortality...
  10. doi request reprint The new chemical biology of nitrite reactions with hemoglobin: R-state catalysis, oxidative denitrosylation, and nitrite reductase/anhydrase
    Mark T Gladwin
    Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Acc Chem Res 42:157-67. 2009
    ..We consider how these reactions may contribute to physiological and pathological hypoxic signaling...
  11. pmc Nitric oxide for inhalation in the acute treatment of sickle cell pain crisis: a randomized controlled trial
    Mark T Gladwin
    Division of Pulmonary, Allergy, and Critical Care Medicine, Vascular Medicine Institute, University of Pittsburgh, 3459 Fifth Ave, 628 NW, Pittsburgh, PA 15213
    JAMA 305:893-902. 2011
    ..Inhaled nitric oxide has shown evidence of efficacy in mouse models of sickle cell disease (SCD), case series of patients with acute chest syndrome, and 2 small placebo-controlled trials for treatment of vaso-occlusive pain crisis (VOC)...
  12. pmc Nitrite reductase activity of hemoglobin as a systemic nitric oxide generator mechanism to detoxify plasma hemoglobin produced during hemolysis
    Peter C Minneci
    Department of Surgery, The Children s Institute for Surgical Science, The Children s Hospital of Philadelphia, PA, USA
    Am J Physiol Heart Circ Physiol 295:H743-54. 2008
    ..Nitrite infusions may promote NO generation from Hb while maintaining oxygen delivery; this effect could be harnessed to treat hemolytic conditions and to detoxify Hb-based blood substitutes...
  13. ncbi request reprint Nitrite infusion in humans and nonhuman primates: endocrine effects, pharmacokinetics, and tolerance formation
    Andre Dejam
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Circulation 116:1821-31. 2007
    ..However, important questions of potency, kinetics, mechanism of action, and possible induction of tolerance remain unanswered...
  14. pmc 14-3-3 binding and phosphorylation of neuroglobin during hypoxia modulate six-to-five heme pocket coordination and rate of nitrite reduction to nitric oxide
    Thottala Jayaraman
    Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 286:42679-89. 2011
    ..Hypoxia-regulated reactions of nitrite and neuroglobin may contribute to the cellular adaptation to hypoxia...
  15. pmc Circulating blood endothelial nitric oxide synthase contributes to the regulation of systemic blood pressure and nitrite homeostasis
    Katherine C Wood
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Arterioscler Thromb Vasc Biol 33:1861-71. 2013
    ..A functional red cell eNOS that modulates vascular NO• signaling has been proposed...
  16. pmc Hemodynamic and functional assessment of patients with sickle cell disease and pulmonary hypertension
    Anastasia Anthi
    Vascular Medicine Branch, Clinical Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 2089, USA
    Am J Respir Crit Care Med 175:1272-9. 2007
    ..Although pulmonary hypertension (PH) is a common complication of sickle cell disease (SCD) associated with high mortality, there exist few data characterizing hemodynamics and cardiopulmonary function in this population...
  17. pmc Hematologic, biochemical, and cardiopulmonary effects of L-arginine supplementation or phosphodiesterase 5 inhibition in patients with sickle cell disease who are on hydroxyurea therapy
    Jane A Little
    Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, NIH, Bethesda, MD 20892 1476, USA
    Eur J Haematol 82:315-21. 2009
    ..L-arginine, an NO precursor, and the phosphodiesterase (PDE) 5 inhibitor sildenafil, which potentiates cGMP, were studied in adults with sickle cell disease (SCD) who were stably on HU...
  18. pmc Angeli's salt counteracts the vasoactive effects of elevated plasma hemoglobin
    Steven B Solomon
    Critical Care Medicine Department, National Institutes of Health, Bethesda, MD 20892, USA Electronic address
    Free Radic Biol Med 53:2229-39. 2012
    ..Increases in metHb in the red blood cell, the potential of AS for neurotoxicity, and hypotension would need to be carefully monitored in a clinical trial. ..
  19. pmc Biological activity of nitric oxide in the plasmatic compartment
    Xunde Wang
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Proc Natl Acad Sci U S A 101:11477-82. 2004
    ..The studies further suggest that alternative NO reaction end products in plasma, such as nitrite, N-nitrosamines, iron-nitrosyls, and nitrated lipids, should be evaluated in blood NO transport along the vasculature...
  20. pmc Evolution of novel small-molecule therapeutics targeting sickle cell vasculopathy
    Gregory J Kato
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, 10 Center Dr, MSC 1476, Bldg 10 CRC, Room 5 5140, Bethesda, MD 20892 1476, USA
    JAMA 300:2638-46. 2008
    ..This article reviews the pathophysiology of sickle vasculopathy and the results of preliminary clinical trials of novel small-molecule therapeutics directed at abnormal vascular biology in patients with sickle cell disease...
  21. ncbi request reprint Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxyhemoglobin
    Rozalina Grubina
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:12916-27. 2007
    ....
  22. pmc Platelet activation in patients with sickle disease, hemolysis-associated pulmonary hypertension, and nitric oxide scavenging by cell-free hemoglobin
    Jose Villagra
    Vascular Medicine Branch of National Heart, Lung, and Blood Institute, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1476, USA
    Blood 110:2166-72. 2007
    ..This supports a role for NO-based therapeutics for SCD vasculopathy. This trial was registered at www.clinicaltrials.gov as no. NCT00352430...
  23. pmc Severity of pulmonary hypertension during vaso-occlusive pain crisis and exercise in patients with sickle cell disease
    Roberto F Machado
    Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1476, USA
    Br J Haematol 136:319-25. 2007
    ..001) in all subjects. These data suggest that acute elevations in pulmonary pressures during VOC or exercise may contribute to morbidity and mortality in patients with sickle cell disease...
  24. ncbi request reprint Ceruloplasmin is a NO oxidase and nitrite synthase that determines endocrine NO homeostasis
    Sruti Shiva
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Chem Biol 2:486-93. 2006
    ....
  25. pmc Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control
    Zhi Huang
    Vascular Therapeutics Section, Cardiovascular Branch, National Heart, Lung and Blood Institute, and Laboratory of Chemical Biology, National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892 1662, USA
    J Clin Invest 115:2099-107. 2005
    ..These data suggest that the oxygen sensor for hypoxic vasodilation is determined by Hb oxygen saturation and quaternary structure and that the nitrite reductase activity of Hb generates NO gas under allosteric and pH control...
  26. pmc Dietary nitrate and nitrite modulate blood and organ nitrite and the cellular ischemic stress response
    Nicolaas J H Raat
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 47:510-7. 2009
    ....
  27. ncbi request reprint Nitrite reductase activity of hemoglobin S (sickle) provides insight into contributions of heme redox potential versus ligand affinity
    Rozalina Grubina
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:3628-38. 2008
    ..From a pathophysiological standpoint, under conditions where HbS polymers form, the rate of nitrite reduction is reduced compared with HbA and solution-phase HbS, indicating that HbS polymers reduce nitrite more slowly...
  28. pmc Nitrite anion provides potent cytoprotective and antiapoptotic effects as adjunctive therapy to reperfusion for acute myocardial infarction
    Felix M Gonzalez
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1061, USA
    Circulation 117:2986-94. 2008
    ....
  29. pmc Amplified expression profiling of platelet transcriptome reveals changes in arginine metabolic pathways in patients with sickle cell disease
    Nalini Raghavachari
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute National Institutes of Health, Bethesda, MD 20892 1454, USA
    Circulation 115:1551-62. 2007
    ..Recent functional studies of platelets in patients with sickle cell disease reveal a basally activated state, which suggests that pathological platelet activation may contribute to sickle cell disease vasculopathy...
  30. pmc In vivo reduction of cell-free methemoglobin to oxyhemoglobin results in vasoconstriction in canines
    Dong Wang
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland Division of Pulmonary, Allergy and Critical Care Medicine and the Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania Department of Physics and the Translational Science Center, Wake Forest University, Winston Salem, North Carolina Anesthesia and Critical Care Medicine Department, West China Hospital of Sichuan University, Cheng Du, China Critical Care Medicine Department, Hospital Universitario de Getafe, Getafe, Madrid, Spain
    Transfusion 53:3149-63. 2013
    ..To clarify this, we tested the vascular effect and fate of oxyHb or metHb infusions...
  31. ncbi request reprint Interferon-gamma induces p11 gene and protein expression in human epithelial cells through interferon-gamma-activated sequences in the p11 promoter
    Xiu li Huang
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:9298-308. 2003
    ....
  32. ncbi request reprint Deoxymyoglobin is a nitrite reductase that generates nitric oxide and regulates mitochondrial respiration
    Sruti Shiva
    Vascular Medicine Branch, Clinical Center National Institutes of Health, Bethesda, MD 20892 1662, USA
    Circ Res 100:654-61. 2007
    ....
  33. pmc Sodium nitrite promotes regional blood flow in patients with sickle cell disease: a phase I/II study
    A Kyle Mack
    Pulmonary and Vascular Medicine Branch, National Heart, Lung and Blood Institute National Institutes of Health, Bethesda, MD, USA
    Br J Haematol 142:971-8. 2008
    ..The unique pharmacological properties of nitrite as a hypoxia-potentiated vasodilator and cytoprotective agent in the setting of ischaemia-reperfusion injury make this anion a plausible NO donor for future clinical trials in SCD...
  34. pmc Segmentation and quantification of pulmonary artery for noninvasive CT assessment of sickle cell secondary pulmonary hypertension
    Marius George Linguraru
    Radiology and Imaging Sciences, Clinical Center National Institutes of Health, Bethesda, Maryland 20892, USA
    Med Phys 37:1522-32. 2010
    ..The authors propose a semiautomated computer-assisted diagnostic (CAD) tool to quantify the main PA size from pulmonary CT angiography (CTA)...
  35. pmc Nitrite Reductase and Nitric-oxide Synthase Activity of the Mitochondrial Molybdopterin Enzymes mARC1 and mARC2
    Courtney E Sparacino-Watkins
    From the Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
    J Biol Chem 289:10345-58. 2014
    ..The reduction of nitrite by mARC in the mitochondria may represent a new signaling pathway for NADH-dependent hypoxic NO production. ..
  36. ncbi request reprint Abnormal pulmonary function and associated risk factors in children and adolescents with sickle cell anemia
    Manuel Arteta
    Department of Medicine, University of Michigan, Ann Arbor, MI Center for Sickle Cell Disease, Howard University Department of Pediatrics, Children s National Medical Center, Washington, DC Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD Department of Medicine, University of Pittsburgh, Pittsburgh, PA Sickle Cell Center, University of Illinois, Chicago, IL
    J Pediatr Hematol Oncol 36:185-9. 2014
    ..Full pulmonary function testing should be performed in children with hemoglobin SS or Sβ(0)-thalassemia, especially with history of asthma or wheezing and accentuated elevations in hemolytic markers...
  37. pmc Plasma thrombospondin-1 is increased during acute sickle cell vaso-occlusive events and associated with acute chest syndrome, hydroxyurea therapy, and lower hemolytic rates
    Enrico M Novelli
    Division of Hematology Oncology, University of Pittsburgh Medical Center UPMC, Vascular Medicine Institute VMI, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Am J Hematol 87:326-30. 2012
    ..Our results suggest that TSP1 levels are associated with more VOE, hydroxyurea use and lower rates of hemolysis. High TSP1 concentrations may indicate higher risk of the viscosity/vaso-occlusion phenotype of SCD...
  38. pmc Erythrocytes are the major intravascular storage sites of nitrite in human blood
    Andre Dejam
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 106:734-9. 2005
    ..These studies suggest a dynamic intravascular NO metabolism in which endothelial NOS-derived NO is stabilized as nitrite, transported by erythrocytes, and consumed during arterial-to-venous transit...
  39. pmc Endothelin receptor antagonists for pulmonary hypertension in adult patients with sickle cell disease
    Caterina P Minniti
    Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Br J Haematol 147:737-43. 2009
    ..Therapy was stopped in two patients who were switched then to the other ETR blocker agent. These data suggest preliminary evidence for the benefit of bosentan and ambrisentan in pulmonary hypertension in SCD...
  40. ncbi request reprint Divergent nitric oxide bioavailability in men and women with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Circulation 107:271-8. 2003
    ..Although reduced endothelial nitric oxide (NO) bioavailability has been demonstrated in arteriosclerotic vascular disease, the integrity of this system in sickle cell disease remains uncertain...
  41. pmc Washing older blood units before transfusion reduces plasma iron and improves outcomes in experimental canine pneumonia
    Irene Cortés-Puch
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD
    Blood 123:1403-11. 2014
    ..Our data suggest that fresh blood should not be washed routinely because, in a setting of established infection, washed RBC are prone to release CFH and result in worsened clinical outcomes. ..
  42. ncbi request reprint Inhaled nebulized nitrite is a hypoxia-sensitive NO-dependent selective pulmonary vasodilator
    Christian J Hunter
    Critical Care Medicine Department, Clinical Center, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892 1662, USA
    Nat Med 10:1122-7. 2004
    ....
  43. pmc Nitric oxide scavenging by red blood cell microparticles and cell-free hemoglobin as a mechanism for the red cell storage lesion
    Chenell Donadee
    Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Circulation 124:465-76. 2011
    ....
  44. pmc Normoxic cyclic GMP-independent oxidative signaling by nitrite enhances airway epithelial cell proliferation and wound healing
    Ling Wang
    Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Nitric Oxide 26:203-10. 2012
    ....
  45. ncbi request reprint Endothelial progenitor cell mobilization and increased intravascular nitric oxide in patients undergoing cardiac rehabilitation
    Jonathan D Paul
    Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    J Cardiopulm Rehabil Prev 27:65-73. 2007
    ....
  46. ncbi request reprint Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilation
    Mark T Gladwin
    Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1454, USA
    Am J Physiol Heart Circ Physiol 291:H2026-35. 2006
    ..An expanded consideration of nitrite as a hypoxia-dependent intrinsic signaling molecule has opened up a new field of research and therapeutic opportunities for diseases associated with regional hypoxia and vasoconstriction...
  47. ncbi request reprint The emerging biology of the nitrite anion
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Chem Biol 1:308-14. 2005
    ..A recent two-day symposium at the US National Institutes of Health highlighted recent advances in the understanding of nitrite biochemistry, physiology and therapeutics...
  48. pmc Increased pulmonary pressures and myocardial wall stress in children with severe malaria
    Jacqueline J Janka
    Clinical Center Critical Care Medicine Department and Pulmonary and Vascular Medicine and Translational Medicine Branches, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Infect Dis 202:791-800. 2010
    ..To test whether this pathophysiology occurs in malaria, we examined in Mali 53 children who were admitted to the hospital with severe malaria (excluding cerebral malaria) and 31 age-matched controls...
  49. doi request reprint Nitrite potently inhibits hypoxic and inflammatory pulmonary arterial hypertension and smooth muscle proliferation via xanthine oxidoreductase-dependent nitric oxide generation
    Brian S Zuckerbraun
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Circulation 121:98-109. 2010
    ..Considering the fact that nitric oxide exhibits vasoprotective properties, we examined the effects of nitrite on experimental pulmonary arterial hypertension...
  50. ncbi request reprint Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation
    Kenyatta Cosby
    Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 7B15 Bethesda, Maryland 20892, USA
    Nat Med 9:1498-505. 2003
    ..These results suggest that nitrite represents a major bioavailable pool of NO, and describe a new physiological function for hemoglobin as a nitrite reductase, potentially contributing to hypoxic vasodilation...
  51. pmc Echocardiographic markers of elevated pulmonary pressure and left ventricular diastolic dysfunction are associated with exercise intolerance in adults and adolescents with homozygous sickle cell anemia in the United States and United Kingdom
    Vandana Sachdev
    Cardiovascular and Pulmonary Medicine Branch, National Heart, Lung, and Blood Institute, Bethesda, MD, USA
    Circulation 124:1452-60. 2011
    ....
  52. pmc Hemolysis-associated endothelial dysfunction mediated by accelerated NO inactivation by decompartmentalized oxyhemoglobin
    Peter C Minneci
    Critical Care Medicine Department, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 115:3409-17. 2005
    ....
  53. pmc The detection of the nitrite reductase and NO-generating properties of haemoglobin by mitochondrial inhibition
    Sruti Shiva
    Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA
    Cardiovasc Res 89:566-73. 2011
    ..Here, we characterize the NO-generating properties of Hb using the cGMP-independent and NO-dependent inhibition of mitochondrial cytochrome c oxidase...
  54. pmc Characterization of erythrocytic uptake and release and disposition pathways of nitrite, nitrate, methemoglobin, and iron-nitrosyl hemoglobin in the human circulation
    Yuen Yi Hon
    Clinical Center Pharmacy Department, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    Drug Metab Dispos 38:1707-13. 2010
    ....
  55. pmc Chronic hyper-hemolysis in sickle cell anemia: association of vascular complications and mortality with less frequent vasoocclusive pain
    James G Taylor
    Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 3:e2095. 2008
    ..We have previously reported that intense hemolysis is associated with increased risk of vascular complications in a small cohort of adults with sickle cell disease. These observations have not been validated in other populations...
  56. pmc Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins
    Xunde Wang
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 44:1362-72. 2008
    ..The addition of copper to ascorbate allows for a simple assay modification that dramatically increases sensitivity while maintaining specificity...
  57. pmc Levels of soluble endothelium-derived adhesion molecules in patients with sickle cell disease are associated with pulmonary hypertension, organ dysfunction, and mortality
    Gregory J Kato
    Vascular Therapeutics Section, Cardiovascular Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
    Br J Haematol 130:943-53. 2005
    ..Our data are consistent with steady state levels of soluble adhesion molecules as markers of pulmonary hypertension and risk of death...
  58. ncbi request reprint Role of the red blood cell in nitric oxide homeostasis and hypoxic vasodilation
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung and Blood Institute National Institutes of Health, Bethesda, Maryland 20892 1454, USA
    Adv Exp Med Biol 588:189-205. 2006
    ..The later newly described role of hemoglobin as a nitrite reductase is discussed in the context of hypoxic vasodilation, blood flow regulation and oxygen sensing...
  59. ncbi request reprint The biochemistry of nitric oxide, nitrite, and hemoglobin: role in blood flow regulation
    Mark T Gladwin
    Critical Care Medicine Department, Clinical Center, Laboratory of Chemical Biology, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Free Radic Biol Med 36:707-17. 2004
    ..This newly described role of hemoglobin as a nitrite reductase is discussed in the context of blood flow regulation, oxygen sensing, and nitrite-based therapeutics...
  60. pmc Mutations and polymorphisms in hemoglobin genes and the risk of pulmonary hypertension and death in sickle cell disease
    James G Taylor
    Vascular Medicine Branch, NHLBI, NIH, Bethesda, Maryland 20892 1476, USA
    Am J Hematol 83:6-14. 2008
    ..Despite this protective association, patients with SC who did develop pulmonary hypertension remained at significant risk for death during 49 months of follow-up (Hazard Ratio=8.20, P=0.0057)...
  61. ncbi request reprint Methodologies for the sensitive and specific measurement of S-nitrosothiols, iron-nitrosyls, and nitrite in biological samples
    Benjamin K Yang
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Free Radic Res 37:1-10. 2003
  62. pmc Physiologic changes in a nonhuman primate model of HIV-associated pulmonary arterial hypertension
    M Patricia George
    Department of Immunology, School of Medicine, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15261
    Am J Respir Cell Mol Biol 48:374-81. 2013
    ..These studies establish an important nonhuman primate model of HIV-associated PAH that will be useful in studies of disease pathogenesis and the efficacy of interventions...
  63. ncbi request reprint Lopinavir-ritonavir: effects on endothelial cell function in healthy subjects
    Jessica R Grubb
    Critical Care Medical Department, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Infect Dis 193:1516-9. 2006
    ..Thus, in this small cohort of subjects, short-term treatment with lopinavir-ritonavir does not appear to directly promote endothelial cell dysfunction...
  64. pmc Human neuroglobin functions as a redox-regulated nitrite reductase
    Mauro Tiso
    Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 286:18277-89. 2011
    ..We hypothesize that the six-coordinate heme globin superfamily may subserve a function as primordial hypoxic and redox-regulated NO-signaling proteins...
  65. ncbi request reprint Heme oxygenase-1 deficiency accelerates formation of arterial thrombosis through oxidative damage to the endothelium, which is rescued by inhaled carbon monoxide
    Andrea L True
    National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892, USA
    Circ Res 101:893-901. 2007
    ..Induction of HO-1 may be beneficial in the prevention of thrombosis associated with vascular oxidant stress and inflammation...
  66. ncbi request reprint Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease
    Christopher D Reiter
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 8:1383-9. 2002
    ..Decompartmentalization of hemoglobin and subsequent dioxygenation of nitric oxide may explain the vascular complications shared by acute and chronic hemolytic disorders...
  67. pmc Nitrite augments tolerance to ischemia/reperfusion injury via the modulation of mitochondrial electron transfer
    Sruti Shiva
    Vascular Medicine Branch, National Heart Lung Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 204:2089-102. 2007
    ..These data suggest that nitrite dynamically modulates mitochondrial resilience to reperfusion injury and may represent an effector of the cell-survival program of ischemic preconditioning and the Mediterranean diet...
  68. pmc Low NO concentration dependence of reductive nitrosylation reaction of hemoglobin
    Jesús Tejero
    Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 287:18262-74. 2012
    ..The observed fast reductive nitrosylation observed at low NO concentrations may function to preserve NO bioactivity via primary oxidation of NO to form nitrite or in the presence of nitrite to form N(2)O(3) and S-nitrosothiols...
  69. pmc Diastolic dysfunction is an independent risk factor for death in patients with sickle cell disease
    Vandana Sachdev
    Cardiovascular Branch, Echocardiography Laboratory, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892 1454, USA
    J Am Coll Cardiol 49:472-9. 2007
    ..The goal of this study was to characterize left ventricular diastolic function in the sickle cell disease (SCD) population and to relate echocardiographic measures of dysfunction with pulmonary hypertension and mortality...
  70. pmc Nitrite-generated NO circumvents dysregulated arginine/NOS signaling to protect against intimal hyperplasia in Sprague-Dawley rats
    Matthew J Alef
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Clin Invest 121:1646-56. 2011
    ..The vasoprotective effects of nitrite were counteracted by inhibition of XOR. These data illustrate the importance of nitrite-generated NO as an endogenous adaptive response and as a pathway that can be harnessed for therapeutic benefit...
  71. ncbi request reprint Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: effects of nitric oxide
    Karel J Zuzak
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases NIH, 9000 Rockville Pike, Bethesda, MD 20892 0510, USA
    Am J Physiol Heart Circ Physiol 285:H1183-9. 2003
    ....
  72. doi request reprint Sickle cell disease vasculopathy: a state of nitric oxide resistance
    Katherine C Wood
    Vascular Medicine Branch, National Heart Lung Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 44:1506-28. 2008
    ..New therapeutic strategies to limit intravascular hemolysis and ROS generation and increase NO bioavailability are discussed...
  73. pmc Markers of severe vaso-occlusive painful episode frequency in children and adolescents with sickle cell anemia
    Deepika S Darbari
    Cardiovascular and Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    J Pediatr 160:286-90. 2012
    ....
  74. ncbi request reprint S-Nitrosohemoglobin is unstable in the reductive erythrocyte environment and lacks O2/NO-linked allosteric function
    Mark T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
    J Biol Chem 277:27818-28. 2002
    ....
  75. pmc The functional nitrite reductase activity of the heme-globins
    Mark T Gladwin
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Bethesda, MD, USA
    Blood 112:2636-47. 2008
    ..We will review these reactions in the biologic framework of hypoxic signaling in blood and the heart...
  76. ncbi request reprint Hemolytic anemia-associated pulmonary hypertension in sickle cell disease
    Elaina E Lin
    National Institutes of Health, 31 Center Drive, Bethesda, MD 20892, USA
    Curr Hematol Rep 4:117-25. 2005
    ..Finally, we review suggested therapies including the established treatments and new pulmonary vasodilator and remodeling agents in the management of pulmonary hypertension in hemolytic anemias...
  77. pmc Lactate dehydrogenase as a biomarker of hemolysis-associated nitric oxide resistance, priapism, leg ulceration, pulmonary hypertension, and death in patients with sickle cell disease
    Gregory J Kato
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Dr, MSC 1476, Bldg 10CRC, Rm 5 5140, Bethesda, MD 20892, USA
    Blood 107:2279-85. 2006
    ..We propose that LDH elevation identifies patients with a syndrome of hemolysis-associated NO resistance, endothelial dysfunction, and end-organ vasculopathy...
  78. pmc Deconstructing sickle cell disease: reappraisal of the role of hemolysis in the development of clinical subphenotypes
    Gregory J Kato
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, Critical Care Medicine Department, Clinical Center, National Institutes of Health, 10 Center Drive, Building 10CRC 5 5140, Bethesda, MD 20892 1476, USA
    Blood Rev 21:37-47. 2007
    ..Some of these drugs are now being studied in clinical trials...
  79. pmc Cerebrovascular disease associated with sickle cell pulmonary hypertension
    Gregory J Kato
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1476, USA
    Am J Hematol 81:503-10. 2006
    ..Clinicians should suspect occult cerebrovascular disease in sickle cell patients with pulmonary hypertension...
  80. ncbi request reprint N-terminal pro-brain natriuretic peptide levels and risk of death in sickle cell disease
    Roberto F Machado
    Vascular Medicine Branch, Clinical Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1454, USA
    JAMA 296:310-8. 2006
    ..Levels of N-terminal pro-brain natriuretic peptide (NT-proBNP) provide such information in patients with idiopathic pulmonary arterial hypertension...
  81. doi request reprint Myocardial protection by nitrite: evidence that this reperfusion therapeutic will not be lost in translation
    Shashank S Sinha
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Trends Cardiovasc Med 18:163-72. 2008
    ....
  82. pmc Sildenafil therapy in patients with sickle cell disease and pulmonary hypertension
    Roberto F Machado
    Vascular Therapeutics Section, Cardiovascular Branch, National Heart Lung and Blood Institute, Bethesda, MD, USA
    Br J Haematol 130:445-53. 2005
    ..No episodes of priapism occurred in the three men in the study; two of them were on chronic exchange transfusions and one had erectile dysfunction...
  83. ncbi request reprint An emerging role for nitric oxide in sickle cell disease vascular homeostasis and therapy
    Christopher D Reiter
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
    Curr Opin Hematol 10:99-107. 2003
    ..Possible effects of chronic nitric oxide-based therapies on erythrocyte density, pulmonary artery pressures, and fetal hemoglobin induction deserve study...
  84. pmc Role of the anion nitrite in ischemia-reperfusion cytoprotection and therapeutics
    Cameron Dezfulian
    Vascular Medicine Branch, National Heart Lung Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cardiovasc Res 75:327-38. 2007
    ....
  85. ncbi request reprint Nitrite infusions to prevent delayed cerebral vasospasm in a primate model of subarachnoid hemorrhage
    Ryszard M Pluta
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    JAMA 293:1477-84. 2005
    ..Decreased bioavailability of nitric oxide has been associated with the development of cerebral vasospasm...
  86. pmc Severe pulmonary hypertension in an adolescent with sickle cell disease
    James G Taylor
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1476, USA
    Am J Hematol 83:71-2. 2008
  87. ncbi request reprint Pulmonary hypertension as a risk factor for death in patients with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    N Engl J Med 350:886-95. 2004
    ..The prevalence of pulmonary hypertension in adults with sickle cell disease, the mechanism of its development, and its prospective prognostic significance are unknown...
  88. pmc Pulmonary vascular lesions are common in SIV- and SHIV-env-infected macaques
    M Patricia George
    Department of Medicine, University of Pittsburgh, Pennsylvania 15213, USA
    AIDS Res Hum Retroviruses 27:103-11. 2011
    ..This SHIV-env model provides a new means to investigate HIV-PAH...
  89. pmc Nitrite in pulmonary arterial hypertension: therapeutic avenues in the setting of dysregulated arginine/nitric oxide synthase signalling
    Brian S Zuckerbraun
    Department of Surgery, University of Pittsburgh, NW 607 MUH, 3459 Fifth Avenue, Pittsburgh, PA 15213, USA
    Cardiovasc Res 89:542-52. 2011
    ....
  90. ncbi request reprint Nitric oxide's reactions with hemoglobin: a view through the SNO-storm
    Mark T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 9:496-500. 2003
  91. ncbi request reprint Hemoglobin and the paracrine and endocrine functions of nitric oxide
    Alan N Schechter
    National Institute of Diabetes and Digestive and Kidney Diseases, and the Critical Care Medicine Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1822, USA
    N Engl J Med 348:1483-5. 2003
  92. pmc NO solutions?
    Alan N Schechter
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 109:1149-51. 2002
  93. ncbi request reprint Nitric oxide donor properties of hydroxyurea in patients with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, Bethesda, MD 20892 1662, USA
    Br J Haematol 116:436-44. 2002
    ..The role of NO in the induction of fetal haemoglobin and possible synergy between NO donor therapy and classic cytostatic and differentiating medications should be explored...
  94. ncbi request reprint Emerging role of nitrite in human biology
    Andre Dejam
    Laboratory of Chemical Biology, National Institute of Diabetes, Digestive and Kidney Disease, National Institute of Health, Bethesda, MD 20892, USA
    Blood Cells Mol Dis 32:423-9. 2004
    ..This review focuses on the role of nitrite as a circulating NO donor, its potential as an index of NO synthase (NOS) activity and endothelial function, and discusses potential diagnostic and therapeutic applications...
  95. pmc Haldane, hot dogs, halitosis, and hypoxic vasodilation: the emerging biology of the nitrite anion
    Mark T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
    J Clin Invest 113:19-21. 2004
    ..Nitrite levels in blood reflect NO production from endothelial NO synthase enzymes, and recent data suggest that nitrite contributes to blood flow regulation by reaction with deoxygenated hemoglobin and tissue heme proteins to form NO...
  96. ncbi request reprint Measurement of circulating nitrite and S-nitrosothiols by reductive chemiluminescence
    Peter H MacArthur
    Vascular Medicine Branch, National Heart Lung Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 851:93-105. 2007
    ..The underlying reaction mechanisms, as well as the potential pitfalls of each method are discussed...
  97. pmc Deconstructing endothelial dysfunction: soluble guanylyl cyclase oxidation and the NO resistance syndrome
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, and Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 116:2330-2. 2006
    ..Diseases associated with NO resistance would appear to be ideally suited for therapies directed at restoring redox homeostasis, sGC activity, and NO sensitivity...
  98. ncbi request reprint Methodological vexation about thiol oxidation versus S-nitrosation -- a commentary on "An ascorbate-dependent artifact that interferes with the interpretation of the biotin-switch assay"
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 41:557-61. 2006
  99. pmc Hydroxyurea induces fetal hemoglobin by the nitric oxide-dependent activation of soluble guanylyl cyclase
    Vladan P Cokic
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Clin Invest 111:231-9. 2003
    ..These data provide strong evidence for an NO-derived mechanism for HbF induction by hydroxyurea and suggest possibilities for therapies based on NO-releasing or -potentiating agents...
  100. ncbi request reprint A pharmacogenomic study of atorvastatin effects on eNOS activity: do "statins" modulate blood nitrite levels and intravascular oxidant stress in susceptible individuals?
    Cameron Dezfulian
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Free Radic Biol Med 41:1041-3. 2006
  101. ncbi request reprint Blood mononuclear cell gene expression profiles characterize the oxidant, hemolytic, and inflammatory stress of sickle cell disease
    Maria L Jison
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 104:270-80. 2004
    ..Global transcriptional analysis of circulating leukocytes highlights the intense oxidant and inflammatory nature of steady-state sickle cell disease and provides insight into the broad compensatory responses to vascular injury...

Research Grants3

  1. Storage Lesion in Banked Blood Due to Disruption of Nitric Oxide Homeostasis
    Mark Gladwin; Fiscal Year: 2009
    ..The project investigates the cause of this reduction in nitric oxide bioavailability and explores ways to restore nitric oxide upon red cell transfusion. ..
  2. Myoglobin as a Nitrite Reductase that Regulates Hypoxic Cardiac NO Signaling
    Sruti Shiva; Fiscal Year: 2010
    ..This project will investigate how nitrite and myoglobin work together so that nitrite can be used to protect the heart during a heart attack. ..
  3. Storage Lesion in Banked Blood Due to Disruption of Nitric Oxide Homeostasis
    Daniel B Kim Shapiro; Fiscal Year: 2010
    ..The project investigates the cause of this reduction in nitric oxide bioavailability and explores ways to restore nitric oxide upon red cell transfusion. ..