L I Gilbert

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Daphnia Halloween genes that encode cytochrome P450s mediating the synthesis of the arthropod molting hormone: evolutionary implications
    Kim F Rewitz
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599 3280 USA
    BMC Evol Biol 8:60. 2008
  2. ncbi request reprint Halloween genes encode P450 enzymes that mediate steroid hormone biosynthesis in Drosophila melanogaster
    Lawrence I Gilbert
    The University of North Carolina at Chapel Hill, CB 3280, Coker Hall, Chapel Hill, NC 27599 3280, USA
    Mol Cell Endocrinol 215:1-10. 2004
  3. ncbi request reprint Control of ecdysteroidogenesis: activation and inhibition of prothoracic gland activity
    L I Gilbert
    Department of Biology, University of North Carolina at Chapel Hill 27599 3280, USA
    Invert Neurosci 3:205-16. 1997
  4. ncbi request reprint Dynamic regulation of prothoracic gland ecdysteroidogenesis: Manduca sexta recombinant prothoracicotropic hormone and brain extracts have identical effects
    L I Gilbert
    Department of Biology, Campus Box 3280, Coker Hall, University of North Carolina at Chapel Hill, 27599 3280, Chapel Hill, NC, USA
    Insect Biochem Mol Biol 30:1079-89. 2000
  5. ncbi request reprint Control and biochemical nature of the ecdysteroidogenic pathway
    Lawrence I Gilbert
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Annu Rev Entomol 47:883-916. 2002
  6. ncbi request reprint Cloning of a beta1 tubulin cDNA from an insect endocrine gland: developmental and hormone-induced changes in mRNA expression
    R Rybczynski
    Department of Biology, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell Endocrinol 141:141-51. 1998
  7. ncbi request reprint Activation of an extracellular signal-regulated kinase (ERK) by the insect prothoracicotropic hormone
    R Rybczynski
    Department of Biology, Coker Hall CB 3280, University of North Carolina at Chapel Hill, 27599 3280, USA
    Mol Cell Endocrinol 184:1-11. 2001
  8. ncbi request reprint Woc (without children) gene control of ecdysone biosynthesis in Drosophila melanogaster
    J T Warren
    Department of Biology, Campus Box #3280, University of North Carolina at Chapel Hill, 27599-3280, USA
    Mol Cell Endocrinol 181:1-14. 2001
  9. ncbi request reprint cDNA cloning and expression of a hormone-regulated heat shock protein (hsc 70) from the prothoracic gland of Manduca sexta
    R Rybczynski
    Department of Biology, Coker Hall CB 3280, University of North Carolina at Chapel Hill, NC 27599, Chapel Hill, USA
    Insect Biochem Mol Biol 30:579-89. 2000
  10. ncbi request reprint A molecular genetic approach to the biosynthesis of the insect steroid molting hormone
    Lawrence I Gilbert
    Department of Biology, University of North Carolina, Chapel Hill North Carolina 27599, USA
    Vitam Horm 73:31-57. 2005

Collaborators

  • Kim F Rewitz
  • R Rybczynski
  • Q Song
  • Yutai Li
  • S Nagata
  • Brian Oliver
  • LINDA RESTIFO
  • S K Fellner
  • CARL THUMMEL
  • Stephen M Cohen
  • James T Warren
  • MICHAEL B O'CONNOR
  • Anna Petryk
  • Chantal Dauphin-Villemant
  • Jean Philippe Parvy
  • Guillermo Marques
  • Hajime Ono
  • Michael Jarcho
  • Michael J Parisi
  • Shiheng An
  • J T Warren
  • Xun Huang
  • Zofeyah McBrayer
  • Mary Jane Shimell
  • Tetsuro Shinoda
  • Kyo Itoyama
  • Dagmar Neubueser
  • Melanie J Palmer
  • Jean Marc Jallon
  • John H Malone
  • David Sturgill
  • Vaijayanti Gupta
  • Yu Zhang
  • Songjie Wang
  • Mark Ellersieck
  • Brenda Beerntsen
  • JoAnn Buchanan
  • Robert B Beckstead
  • MaryJane Shimell
  • Matthew P Scott
  • Yoram Yerushalmi
  • Jun Kobayashi
  • Michael P Jarcho
  • Jonathan Kahler
  • Xiaoping Guo
  • Xiaojing Jin
  • B Subrahmanyam
  • J Wismar
  • J D Dai
  • J S Bachmann

Detail Information

Publications31

  1. pmc Daphnia Halloween genes that encode cytochrome P450s mediating the synthesis of the arthropod molting hormone: evolutionary implications
    Kim F Rewitz
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599 3280 USA
    BMC Evol Biol 8:60. 2008
    ..In insects, the CYP enzymes mediating 20E biosynthesis have been identified, but evidence of their probable presence in crustaceans is indirect, relying solely on the ability of crustaceans to synthesize 20E...
  2. ncbi request reprint Halloween genes encode P450 enzymes that mediate steroid hormone biosynthesis in Drosophila melanogaster
    Lawrence I Gilbert
    The University of North Carolina at Chapel Hill, CB 3280, Coker Hall, Chapel Hill, NC 27599 3280, USA
    Mol Cell Endocrinol 215:1-10. 2004
    ..Shade is present in several tissues as expected while disembodied and shadow are restricted to the ring gland. The paradigm used should allow us to define the enzymes mediating the entire ecdysteroid biosynthetic pathway...
  3. ncbi request reprint Control of ecdysteroidogenesis: activation and inhibition of prothoracic gland activity
    L I Gilbert
    Department of Biology, University of North Carolina at Chapel Hill 27599 3280, USA
    Invert Neurosci 3:205-16. 1997
    ..g. feedback inhibition) by controlling the levels of enzymes or other proteins in the ecdysteroid biosynthetic pathway...
  4. ncbi request reprint Dynamic regulation of prothoracic gland ecdysteroidogenesis: Manduca sexta recombinant prothoracicotropic hormone and brain extracts have identical effects
    L I Gilbert
    Department of Biology, Campus Box 3280, Coker Hall, University of North Carolina at Chapel Hill, 27599 3280, Chapel Hill, NC, USA
    Insect Biochem Mol Biol 30:1079-89. 2000
    ....
  5. ncbi request reprint Control and biochemical nature of the ecdysteroidogenic pathway
    Lawrence I Gilbert
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Annu Rev Entomol 47:883-916. 2002
    ....
  6. ncbi request reprint Cloning of a beta1 tubulin cDNA from an insect endocrine gland: developmental and hormone-induced changes in mRNA expression
    R Rybczynski
    Department of Biology, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell Endocrinol 141:141-51. 1998
    ..The data also support our earlier proposal that the prothoracic gland beta1 tubulin gene is ubiquitously expressed but exhibits tissue- and developmental-specific regulation of transcription and translation...
  7. ncbi request reprint Activation of an extracellular signal-regulated kinase (ERK) by the insect prothoracicotropic hormone
    R Rybczynski
    Department of Biology, Coker Hall CB 3280, University of North Carolina at Chapel Hill, 27599 3280, USA
    Mol Cell Endocrinol 184:1-11. 2001
    ..PTTH-stimulated ERK activity may be important for both rapid regulation of ecdysteroid synthesis and for longer-term changes in the size and function of prothoracic gland cells...
  8. ncbi request reprint Woc (without children) gene control of ecdysone biosynthesis in Drosophila melanogaster
    J T Warren
    Department of Biology, Campus Box #3280, University of North Carolina at Chapel Hill, 27599-3280, USA
    Mol Cell Endocrinol 181:1-14. 2001
    ..Data previously reported (Wismar et al., 2000) indicate that the woc gene encodes a zinc-finger protein that apparently modulates the activity of the 7,8-dehydrogenase...
  9. ncbi request reprint cDNA cloning and expression of a hormone-regulated heat shock protein (hsc 70) from the prothoracic gland of Manduca sexta
    R Rybczynski
    Department of Biology, Coker Hall CB 3280, University of North Carolina at Chapel Hill, NC 27599, Chapel Hill, USA
    Insect Biochem Mol Biol 30:579-89. 2000
    ..The protein and mRNA data suggest that hsc 70 could be involved in a negative feedback loop regulating assembly of the ecdysone receptor complex...
  10. ncbi request reprint A molecular genetic approach to the biosynthesis of the insect steroid molting hormone
    Lawrence I Gilbert
    Department of Biology, University of North Carolina, Chapel Hill North Carolina 27599, USA
    Vitam Horm 73:31-57. 2005
    ..Further, the sequence of these hydroxylations has been determined, and developmental alterations in the expression of each of these genes have been quantified during both embryonic and postembryonic life...
  11. ncbi request reprint Differential incorporation of cholesterol and cholesterol derivatives into ecdysteroids by the larval ring glands and adult ovaries of Drosophila melanogaster: a putative explanation for the l(3)ecd1 mutation
    J T Warren
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Insect Biochem Mol Biol 26:931-43. 1996
    ....
  12. ncbi request reprint Prothoracicotropic hormone stimulated extracellular signal-regulated kinase (ERK) activity: the changing roles of Ca(2+)- and cAMP-dependent mechanisms in the insect prothoracic glands during metamorphosis
    R Rybczynski
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Mol Cell Endocrinol 205:159-68. 2003
    ..The data suggest that the role or importance of components of the PTTH signal transduction cascade are not fixed and can change during development...
  13. ncbi request reprint Ca2+ signaling in prothoracicotropic hormone-stimulated prothoracic gland cells of Manduca sexta: evidence for mobilization and entry mechanisms
    Susan K Fellner
    Department of Cell and Molecular Physiology CB 7545, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Insect Biochem Mol Biol 35:263-75. 2005
    ..We propose that PTTH initiates a transductory cascade typical of many G-protein coupled receptors, involving both Ca2+ mobilization and entry pathways...
  14. pmc Molecular and biochemical characterization of two P450 enzymes in the ecdysteroidogenic pathway of Drosophila melanogaster
    James T Warren
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599 3280, USA
    Proc Natl Acad Sci U S A 99:11043-8. 2002
    ..Expression of dib and sad continues to be localized in this endocrine compartment during larval development, being maximal in both the late second and third instar larvae, about the time of the premolt peaks in the ecdysteroid titer...
  15. pmc Discrete pulses of molting hormone, 20-hydroxyecdysone, during late larval development of Drosophila melanogaster: correlations with changes in gene activity
    James T Warren
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Dev Dyn 235:315-26. 2006
    ..The possible regulation of 20E levels by biosynthetic P450 enzymes and the roles of these early peaks in coordinating gene expression and late larval development are discussed...
  16. ncbi request reprint Protein kinase C modulates ecdysteroidogenesis in the prothoracic gland of the tobacco hornworm, Manduca sexta
    Robert Rybczynski
    Department of Biology, University of North Carolina at Chapel Hill, 27599 3280, USA
    Mol Cell Endocrinol 251:78-87. 2006
    ..These observations reveal that the acute regulation of prothoracic gland steroidogenesis is dependent on a web of interacting kinase pathways, which probably converge on factors that regulate translation...
  17. ncbi request reprint Phantom encodes the 25-hydroxylase of Drosophila melanogaster and Bombyx mori: a P450 enzyme critical in ecdysone biosynthesis
    James T Warren
    Department of Biology, University of North Carolina, 342 Wilson Hall, CB 3280, Chapel Hill, NC 27599 3280, USA
    Insect Biochem Mol Biol 34:991-1010. 2004
    ....
  18. doi request reprint Studies on the Black Box: incorporation of 3-oxo-7-dehydrocholesterol into ecdysteroids by Drosophila melanogaster and Manduca sexta
    James T Warren
    Department of Biology, Campus Box 3280, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Insect Biochem Mol Biol 39:677-87. 2009
    ....
  19. doi request reprint Manduca sexta prothoracicotropic hormone: evidence for a role beyond steroidogenesis
    Robert Rybczynski
    Department of Biology, CB 3280, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Arch Insect Biochem Physiol 70:217-29. 2009
    ..The data indicate that PTTH indeed plays a role in non-steroidogenic tissues and suggest possible future avenues for determining which cellular processes are being so regulated...
  20. ncbi request reprint Profiling of ecdysteroids in complex biological samples using liquid chromatography/ion trap mass spectrometry
    Yutai Li
    Department of Environmental Sciences and Engineering, School of Public Health, The University of North Carolina at Chapel Hill, NC 27599, USA
    Rapid Commun Mass Spectrom 20:185-92. 2006
    ....
  21. pmc Germline-dependent gene expression in distant non-gonadal somatic tissues of Drosophila
    Michael J Parisi
    Department of Biology, University of Pennsylvania, Philadelphia, PA, USA
    BMC Genomics 11:346. 2010
    ..We know little about the coordinated regulation of gene expression patterns in distant somatic tissues that support the developmental cost of gamete production...
  22. ncbi request reprint Identification, characterization and developmental expression of Halloween genes encoding P450 enzymes mediating ecdysone biosynthesis in the tobacco hornworm, Manduca sexta
    Kim F Rewitz
    Department of Life Sciences and Chemistry, Roskilde University, P O Box 260, 4000 Roskilde, Denmark
    Insect Biochem Mol Biol 36:188-99. 2006
    ..The consistent expression of the Halloween genes confirms the importance of the prothoracic glands in pupal-adult development. These studies establish Manduca as an excellent model for examining the regulation of the Halloween genes...
  23. pmc Prothoracicotropic hormone regulates developmental timing and body size in Drosophila
    Zofeyah McBrayer
    The Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Dev Cell 13:857-71. 2007
    ..These results indicate that final body size in insects is determined by a balance between growth-rate regulators such as insulin and developmental timing cues such as PTTH that set the duration of the feeding interval...
  24. ncbi request reprint Drosophila Niemann-Pick type C-2 genes control sterol homeostasis and steroid biosynthesis: a model of human neurodegenerative disease
    Xun Huang
    Department of Developmental Biology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305 5439, USA
    Development 134:3733-42. 2007
    ..Moreover, npc2a; npc2b double mutants undergo apoptotic neurodegeneration, thus constituting a new fly model of human neurodegenerative disease...
  25. ncbi request reprint Molecular evolution of the insect Halloween family of cytochrome P450s: phylogeny, gene organization and functional conservation
    Kim F Rewitz
    Department of Science, Systems and Models, Roskilde University, P O Box 260, 4000 Roskilde, Denmark
    Insect Biochem Mol Biol 37:741-53. 2007
    ..The results suggest that duplications of ancestral P450 genes that acquired novel functions may have been an important mechanism for evolving the ecdysteroidogenic pathway...
  26. ncbi request reprint Spook and Spookier code for stage-specific components of the ecdysone biosynthetic pathway in Diptera
    Hajime Ono
    The Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Dev Biol 298:555-70. 2006
    ..These studies suggest an evolutionary split between Diptera and Lepidoptera in how the ecdysone biosynthetic pathway is regulated during development...
  27. ncbi request reprint molting defective is required for ecdysone biosynthesis
    Dagmar Neubueser
    European Molecular Biology Laboratory, Heidelberg, Germany
    Dev Biol 280:362-72. 2005
    ..Here, we report identification of one such gene, which we call molting defective, on the basis of its lethal phenotype. molting defective encodes a nuclear zinc finger protein required for ecdysone biosynthesis...
  28. ncbi request reprint Developmental expression of Manduca shade, the P450 mediating the final step in molting hormone synthesis
    Kim F Rewitz
    Department of Life Sciences and Chemistry, Roskilde University, P O Box 260, 4000 Roskilde, Denmark
    Mol Cell Endocrinol 247:166-74. 2006
    ..The data suggest that precise tissue-specific transcriptional regulation controls the levels, and thereby the activity, of the Manduca E20MO...
  29. pmc Global identification of bursicon-regulated genes in Drosophila melanogaster
    Shiheng An
    Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA
    BMC Genomics 9:424. 2008
    ..However, little is known regarding the genes that are regulated by bursicon. The identification of bursicon-regulated genes is the focus of this investigation...
  30. pmc Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone
    Anna Petryk
    Department of Pediatrics, University of Minnesota, 516 Delaware Street SE, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:13773-8. 2003
    ....
  31. ncbi request reprint Developmental profiles of ecdysteroids, ecdysteroid receptor mRNAs and DNA binding properties of ecdysteroid receptors in the Ixodid tick Amblyomma americanum (L.)
    Melanie J Palmer
    Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater 74078 3033, USA
    Insect Biochem Mol Biol 32:465-76. 2002
    ..These data suggest that functional differences may exist between the AamRXR1 and AamRXR2 proteins...