C M Giachelli

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint Ectopic calcification: new concepts in cellular regulation
    C M Giachelli
    Bioengineering Department, Box 351720, University of Washington, Seattle, WA 98195, USA
    Z Kardiol 90:31-7. 2001
  2. pmc Inactivation of the osteopontin gene enhances vascular calcification of matrix Gla protein-deficient mice: evidence for osteopontin as an inducible inhibitor of vascular calcification in vivo
    Mei Y Speer
    Bioengineering Department, University of Washington, Seattle 98195, USA
    J Exp Med 196:1047-55. 2002
  3. pmc Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet
    Wei Ling Lau
    Department of Nephrology, University of Washington, Seattle, Washington 98195, USA
    Kidney Int 82:1261-70. 2012
  4. pmc High phosphate feeding promotes mineral and bone abnormalities in mice with chronic kidney disease
    Wei Ling Lau
    Department of Nephrology, University of Washington, Seattle, WA, USA
    Nephrol Dial Transplant 28:62-9. 2013
  5. ncbi request reprint Inducers and inhibitors of biomineralization: lessons from pathological calcification
    C M Giachelli
    Department of Bioengineering, University of Washington, Seattle, 98195, USA
    Orthod Craniofac Res 8:229-31. 2005
  6. ncbi request reprint Regulation of vascular calcification: roles of phosphate and osteopontin
    Cecilia M Giachelli
    Bioengineering Department, University of Washington, Seattle, Wash 98195, USA
    Circ Res 96:717-22. 2005
  7. ncbi request reprint Mechanisms of vascular calcification in uremia
    Cecilia M Giachelli
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Semin Nephrol 24:401-2. 2004
  8. ncbi request reprint Vascular calcification mechanisms
    Cecilia M Giachelli
    Bioengineering Department, Box 351720, University of Washington, Okanogan Lane, Bagley Hall, Seattle, WA 98195, USA
    J Am Soc Nephrol 15:2959-64. 2004
  9. ncbi request reprint Vascular calcification and inorganic phosphate
    C M Giachelli
    Department of Bioengineering, University of Washington, Seattle, WA, USA
    Am J Kidney Dis 38:S34-7. 2001
  10. ncbi request reprint Vascular calcification: in vitro evidence for the role of inorganic phosphate
    Cecilia M Giachelli
    Bioengineering Department, University of Washington, Seattle, Washington 98194 1720, USA
    J Am Soc Nephrol 14:S300-4. 2003

Research Grants

  1. Calcification and Vascular Cell Differentiation
    Cecilia Giachelli; Fiscal Year: 2009
  2. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2000
  3. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2009
  4. Calcification and Vascular Cell Differentiation
    Cecilia Giachelli; Fiscal Year: 2007
  5. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2007
  6. Calcification and Vascular Cell Differentiation
    Cecilia Giachelli; Fiscal Year: 2006
  7. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2006
  8. Calcification and Vascular Cell Differentiation
    Cecilia Giachelli; Fiscal Year: 2005
  9. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2005
  10. Common Mediators of Vascular Calcification and Bone Dis*
    Cecilia Giachelli; Fiscal Year: 2004

Collaborators

Detail Information

Publications80

  1. ncbi request reprint Ectopic calcification: new concepts in cellular regulation
    C M Giachelli
    Bioengineering Department, Box 351720, University of Washington, Seattle, WA 98195, USA
    Z Kardiol 90:31-7. 2001
    ..These studies suggest that both constitutively expressed (surveillance) and inducible (damage control) mineralization inhibitory molecules are produced by mesenchymal and inflammatory cells to control ectopic calcification...
  2. pmc Inactivation of the osteopontin gene enhances vascular calcification of matrix Gla protein-deficient mice: evidence for osteopontin as an inducible inhibitor of vascular calcification in vivo
    Mei Y Speer
    Bioengineering Department, University of Washington, Seattle 98195, USA
    J Exp Med 196:1047-55. 2002
    ..These studies are the first to demonstrate a role for OPN as an inducible inhibitor of ectopic calcification in vivo...
  3. pmc Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet
    Wei Ling Lau
    Department of Nephrology, University of Washington, Seattle, Washington 98195, USA
    Kidney Int 82:1261-70. 2012
    ..Thus, klotho and osteopontin were upregulated by VDRA therapy in chronic kidney disease, independent of changes in serum parathyroid hormone and calcium...
  4. pmc High phosphate feeding promotes mineral and bone abnormalities in mice with chronic kidney disease
    Wei Ling Lau
    Department of Nephrology, University of Washington, Seattle, WA, USA
    Nephrol Dial Transplant 28:62-9. 2013
    ..The mechanisms underlying this syndrome in individuals with chronic kidney disease (CKD) are not yet clear...
  5. ncbi request reprint Inducers and inhibitors of biomineralization: lessons from pathological calcification
    C M Giachelli
    Department of Bioengineering, University of Washington, Seattle, 98195, USA
    Orthod Craniofac Res 8:229-31. 2005
    ..Over the past several years, we have developed in vitro and in vivo models of ectopic calcification to investigate potential inducers and inhibitors of this process...
  6. ncbi request reprint Regulation of vascular calcification: roles of phosphate and osteopontin
    Cecilia M Giachelli
    Bioengineering Department, University of Washington, Seattle, Wash 98195, USA
    Circ Res 96:717-22. 2005
    ..Osteopontin, however, appears to block vascular calcification most likely by preventing calcium phosphate crystal growth and inducing cellular mineral resorption...
  7. ncbi request reprint Mechanisms of vascular calcification in uremia
    Cecilia M Giachelli
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Semin Nephrol 24:401-2. 2004
    ..Thus, in addition to contributing to increased calcium x phosphate product (Ca x P), hyperphosphatemia may have direct effects on SMCs that predispose these cells to calcium deposition in end-stage renal disease patients...
  8. ncbi request reprint Vascular calcification mechanisms
    Cecilia M Giachelli
    Bioengineering Department, Box 351720, University of Washington, Okanogan Lane, Bagley Hall, Seattle, WA 98195, USA
    J Am Soc Nephrol 15:2959-64. 2004
    ..Whether or not vascular calcification can be reversed is not yet known, but exciting new studies suggest that this may be possible in the future...
  9. ncbi request reprint Vascular calcification and inorganic phosphate
    C M Giachelli
    Department of Bioengineering, University of Washington, Seattle, WA, USA
    Am J Kidney Dis 38:S34-7. 2001
    ....
  10. ncbi request reprint Vascular calcification: in vitro evidence for the role of inorganic phosphate
    Cecilia M Giachelli
    Bioengineering Department, University of Washington, Seattle, Washington 98194 1720, USA
    J Am Soc Nephrol 14:S300-4. 2003
    ..From a clinical viewpoint, it seems that early control or prevention of hyperphosphatemia may reduce coronary calcification and its associated morbidity and mortality for patients on dialysis...
  11. ncbi request reprint Osteopontin: a versatile regulator of inflammation and biomineralization
    C M Giachelli
    Department of Bioengineering, University of Washington, Seattle, WA, 98195, USA
    Matrix Biol 19:615-22. 2000
    ....
  12. pmc Evidence for a role of osteopontin in macrophage infiltration in response to pathological stimuli in vivo
    C M Giachelli
    Department of Pathology, University of Washington, Seattle 98195, USA
    Am J Pathol 152:353-8. 1998
    ..These data support the hypothesis that osteopontin may be a critical mediator of inflammation in specific disease and injury states, potentially by promoting macrophage adhesion and migration...
  13. pmc The emerging role of phosphate in vascular calcification
    Cecilia M Giachelli
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Kidney Int 75:890-7. 2009
    ..Disease states and cytokines can increase expression of sodium-dependent phosphate cotransporters in SMCs, thereby increasing susceptibility to calcification even at phosphate concentrations that are in the normal range...
  14. ncbi request reprint Osteopontin N-terminal domain contains a cryptic adhesive sequence recognized by alpha9beta1 integrin
    L L Smith
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 271:28485-91. 1996
    ..We also demonstrate that osteopontin contains a cryptic binding activity, which can be recognized by a novel osteopontin receptor. This receptor has been identified as the alpha9beta1 integrin...
  15. pmc NF-kappaB mediates alphavbeta3 integrin-induced endothelial cell survival
    M Scatena
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    J Cell Biol 141:1083-93. 1998
    ..In contrast, inhibition of MEK and PI3-kinase did not affect osteopontin-induced NF-kappaB activation. These studies identify NF-kappaB as an important signaling molecule in alphavbeta3 integrin-mediated endothelial cell survival...
  16. ncbi request reprint Osteopontin expression in cardiovascular diseases
    C M Giachelli
    Department of Pathology SJ 60, University of Washington, School of Medicine, Seattle 98195, USA
    Ann N Y Acad Sci 760:109-26. 1995
    ....
  17. ncbi request reprint Smooth muscle cell phenotypic transition associated with calcification: upregulation of Cbfa1 and downregulation of smooth muscle lineage markers
    S A Steitz
    Department of Bioengineering, University of Washington, Seattle, Washington, USA
    Circ Res 89:1147-54. 2001
    ..The findings suggest a positive role for SMCs in promoting vascular calcification...
  18. ncbi request reprint Phosphate regulation of vascular smooth muscle cell calcification
    S Jono
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Circ Res 87:E10-7. 2000
    ..The full text of this article is available at http://www.circresaha.org...
  19. ncbi request reprint Obstructive uropathy in the mouse: role of osteopontin in interstitial fibrosis and apoptosis
    V Ophascharoensuk
    Division of Nephrology, Department of Medicine, University of Washington, Seattle 98195, USA
    Kidney Int 56:571-80. 1999
    ..Osteopontin is a macrophage adhesive protein that is expressed by renal tubules in tubulointerstitial disease...
  20. ncbi request reprint Differential expression of alpha 1 type VIII collagen in injured platelet-derived growth factor-BB--stimulated rat carotid arteries
    M P Bendeck
    Department of Pathology, University of Washington, Seattle, USA
    Circ Res 79:524-31. 1996
    ..This molecule constitutes an important component of smooth muscle cell response to vessel injury and may play an important functional role in mediating migration...
  21. ncbi request reprint Cellular proliferation and macrophage influx precede interstitial fibrosis in cyclosporine nephrotoxicity
    B A Young
    Department of Medicine, University of Washington, Seattle, USA
    Kidney Int 48:439-48. 1995
    ..These cellular events may be important in the pathogenesis of chronic CsA nephrotoxicity...
  22. ncbi request reprint Role of beta1 and beta3 integrins in human smooth muscle cell adhesion to and contraction of fibrin clots in vitro
    K O Yee
    Department of Pathology, University of Washington, Seattle 98195, USA
    Circ Res 83:241-51. 1998
    ..This interaction is of potential interest as a target for efforts to block vascular contraction...
  23. ncbi request reprint Osteopontin promotes vascular cell adhesion and spreading and is chemotactic for smooth muscle cells in vitro
    L Liaw
    Department of Pathology, University of Washington, Seattle 98195
    Circ Res 74:214-24. 1994
    ....
  24. ncbi request reprint Chimeric peptides of statherin and osteopontin that bind hydroxyapatite and mediate cell adhesion
    M Gilbert
    Department of Bioengineering and Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 275:16213-8. 2000
    ..This fusion peptide framework may thus provide a straightforward design for immobilizing bioactive sequences on hydroxyapatite for biomaterials, tissue engineering, and vaccine applications...
  25. ncbi request reprint Platelet-derived growth factor-A mRNA expression in fetal, normal adult, and atherosclerotic human aortas. Analysis by competitive polymerase chain reaction
    C E Murry
    Department of Pathology, University of Washington, School of Medicine, Seattle, WA 98195, USA
    Circulation 93:1095-106. 1996
    ..We tested whether platelet-derived growth factor-A (PDGF-A) mRNA levels correlated with cell proliferation in developing aorta, normal adult aorta, and atherosclerotic plaques...
  26. pmc Macrophages express osteopontin during repair of myocardial necrosis
    C E Murry
    Department of Pathology, University of Washington School of Medicine, Seattle 98195
    Am J Pathol 145:1450-62. 1994
    ..These results provide the first evidence that osteopontin may be important in healing after tissue injury, possibly in cellular adhesion, chemotaxis, and/or phagocytosis...
  27. pmc The adhesive and migratory effects of osteopontin are mediated via distinct cell surface integrins. Role of alpha v beta 3 in smooth muscle cell migration to osteopontin in vitro
    L Liaw
    Department of Pathology, University of Washington, Seattle 98195
    J Clin Invest 95:713-24. 1995
    ..Thus, interaction with distinct receptors is one mechanism by which osteopontin may initiate multiple functions...
  28. pmc Characterization of human aortic smooth muscle cells expressing HPV16 E6 and E7 open reading frames
    S C Conroy
    Fred Hutchison Cancer Research Center, Seattle, Washington 98104, USA
    Am J Pathol 147:753-62. 1995
    ....
  29. ncbi request reprint Osteopontin expression in human cyclosporine toxicity
    K L Hudkins
    Department of Pathology, University of Washington, Seattle, Washington, USA
    Kidney Int 60:635-40. 2001
    ..Therefore, osteopontin expression alone is insufficient to serve as the principal mediator of intrarenal monocyte/macrophage influx in the transplant setting...
  30. ncbi request reprint Phosphorylation of osteopontin is required for inhibition of vascular smooth muscle cell calcification
    S Jono
    Department of Bioengineering, University of Washington, Seattle, Washington, 98195, USA
    J Biol Chem 275:20197-203. 2000
    ..These results indicate that phosphorylation is required for the inhibitory effect of OPN on HSMC calcification, and that regulation of OPN phosphorylation represents one way in which mineralization may be controlled by cells...
  31. ncbi request reprint Osteopontin: a multifunctional molecule regulating chronic inflammation and vascular disease
    Marta Scatena
    Department of Bioengineering, University of Washington, Box 355061, Seattle, WA 98195, USA
    Arterioscler Thromb Vasc Biol 27:2302-9. 2007
    ..We believe that further knowledge of the effects of OPN fragments on cell responses might help in designing therapeutics targeting inflammatory and cardiovascular diseases...
  32. ncbi request reprint Crosslinking of an oesophagus acellular matrix tissue scaffold
    Amit D Bhrany
    Department of Otolaryngology Head and Neck Surgery, University of Washington, Box 356515, Seattle, WA 98195, USA
    J Tissue Eng Regen Med 2:365-72. 2008
    ....
  33. ncbi request reprint Mitigation of ectopic calcification in osteopontin-deficient mice by exogenous osteopontin
    Rachit Ohri
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Calcif Tissue Int 76:307-15. 2005
    ....
  34. ncbi request reprint Free fatty acid impairment of nitric oxide production in endothelial cells is mediated by IKKbeta
    Francis Kim
    Department of Medicine, Division of Cardiology, University of Washington, Harborview Medical Center, Seattle, Wash 98104, USA
    Arterioscler Thromb Vasc Biol 25:989-94. 2005
    ..Insulin receptor substrate-1 (IRS-1) regulation is critical for activation of endothelial nitric oxide synthase (eNOS) in response to stimulation by insulin or fluid shear stress...
  35. ncbi request reprint Activation of IKKbeta by glucose is necessary and sufficient to impair insulin signaling and nitric oxide production in endothelial cells
    Francis Kim
    Department of Medicine, Harborview Medical Center, University of Washington, Box 359748, Seattle, WA 98104, USA
    J Mol Cell Cardiol 39:327-34. 2005
    ..These data demonstrate that activation of IKKbeta plays a critical and novel role to mediate the deleterious effects of high glucose on endothelial cell function...
  36. pmc Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin
    Mohga M El-Abbadi
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Kidney Int 75:1297-307. 2009
    ..These findings suggest that phosphate loading and the severity of uremia play critical roles in controlling arterial medial calcification in mice. Further, FGF-23 and osteopontin may be markers and/or inducers of this process...
  37. pmc The role of osteoprotegerin and tumor necrosis factor-related apoptosis-inducing ligand in human microvascular endothelial cell survival
    L B Pritzker
    Department of Bioengineering, University of Washington, Box 351720, Seattle, Washington 98195 1720, USA
    Mol Biol Cell 15:2834-41. 2004
    ..This evidence indicates that OPG blocks endothelial cell apoptosis through binding TRAIL and preventing its interaction with death-inducing TRAIL-receptors..
  38. ncbi request reprint A subpopulation of smooth muscle cells in injured rat arteries expresses platelet-derived growth factor-B chain mRNA
    V Lindner
    University of Washington, Department of Pathology, Seattle 98195, USA
    Circ Res 76:951-7. 1995
    ....
  39. ncbi request reprint Angiotensin II induction of osteopontin expression and DNA replication in rat arteries
    D deBlois
    Department of Pathology and Surgery, University of Washington, Seattle
    Hypertension 28:1055-63. 1996
    ..Osteopontin induction in smooth muscle cells may contribute to Ang II-dependent intimal lesion progression and vascular remodeling events associated with renovascular diseases or hyperadrenergic disorders...
  40. pmc Smooth muscle cells give rise to osteochondrogenic precursors and chondrocytes in calcifying arteries
    Mei Y Speer
    Department of Bioengineering, University of Washington, Box 355061, 1705 NE Pacific St Foege N330L, Seattle, WA 98195, USA
    Circ Res 104:733-41. 2009
    ....
  41. ncbi request reprint Molecular mediators of alphavbeta3-induced endothelial cell survival
    Julie Rice
    Department of Pathology, University of Washington, Seattle 98195 1720, USA
    J Vasc Res 43:422-36. 2006
    ..These studies for the first time show that the alphavbeta3-NF-kappaB-dependent endothelial survival pathway is dependent on IkappaBalpha, IKKbeta, and NIK...
  42. ncbi request reprint Arteriosclerosis, calcium phosphate deposition and cardiovascular disease in uremia: current concepts at the bench
    Mohga El-Abbadi
    Bioengineering Department, University of Washington, Seattle, Washington 98195, USA
    Curr Opin Nephrol Hypertens 14:519-24. 2005
    ..This review highlights important recent findings regarding the etiology of vascular calcification, with special emphasis on pathways that may be particularly relevant in chronic kidney disease patients...
  43. ncbi request reprint The role of osteopontin in foreign body giant cell formation
    Annabel T Tsai
    Department of Bioengineering, University of Washington, Box 351720, Seattle, WA 98195, USA
    Biomaterials 26:5835-43. 2005
    ..In an in vitro human FBGC assay, addition of soluble OPN was found to reduce macrophage fusion to giant cells. These are the first studies to show a direct inhibitory role of OPN in FBGC formation in response to implantation...
  44. ncbi request reprint Sodium-dependent phosphate cotransporters and vascular calcification
    Xianwu Li
    Department of Bioengineering, University of Washington, Seattle, Washington 98195 5061, USA
    Curr Opin Nephrol Hypertens 16:325-8. 2007
    ..This review focuses on the potential role of phosphate transport and type III sodium-dependent phosphate cotransporters in the process of vascular calcification...
  45. ncbi request reprint Mechanisms of vascular calcification
    Mohga El-Abbadi
    Department of Bioengineering, University of Washington, Seattle 98195, WA USA
    Adv Chronic Kidney Dis 14:54-66. 2007
    ..This article reviews current mechanisms proposed for the regulation of vascular calcification and data supporting their potential contribution to this process in chronic kidney disease...
  46. ncbi request reprint Quantitative interrogation of micropatterned biomolecules by surface force microscopy
    M D Garrison
    University of Washington Engineered Biomaterials and Department of Bioengineering, University of Washington, Seattle 98195 1750, USA
    Ultramicroscopy 82:193-202. 2000
    ..The surfaces were imaged via SFM and the specific binding forces between surface associated biomolecules and antibody functionalized tips were quantified...
  47. ncbi request reprint Hyaluronic acid grafting mitigates calcification of glutaraldehyde-fixed bovine pericardium
    Rachit Ohri
    Department of Bioengineering, University of Washington, Box 351720, Bagley Hall, 479, Seattle, Washington 98195
    J Biomed Mater Res A 70:328-34. 2004
    ..005). The calcification-mitigating effect of HA surface modification was also confirmed by microscopic analysis of explanted tissue stained with Alizarin Red S for calcium...
  48. ncbi request reprint Development of an esophagus acellular matrix tissue scaffold
    Amit D Bhrany
    Department of Otolaryngology Head and Neck Surgery, University of Washington, Seattle, USA
    Tissue Eng 12:319-30. 2006
    ....
  49. ncbi request reprint Src kinase activity is required for integrin alphaVbeta3-mediated activation of nuclear factor-kappaB
    Donald L Courter
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 280:12145-51. 2005
    ..Together, these experiments indicate that the C terminus of beta3 and Src activity are required for integrin alpha(v)beta3-mediated nuclear factor-kappaB activation...
  50. pmc Phosphate and vascular calcification: Emerging role of the sodium-dependent phosphate co-transporter PiT-1
    Wei Ling Lau
    Nephrology, University of Washington, Seattle, Washington, USA
    Thromb Haemost 104:464-70. 2010
    ....
  51. ncbi request reprint Characterization and analysis of osteopontin-immobilized poly(2-hydroxyethyl methacrylate) surfaces
    Stephanie M Martin
    Department of Bioengineering, University of Washington, Seattle, Washington, USA
    J Biomed Mater Res A 67:334-43. 2003
    ..The use of multiple tools to characterize the many facets of a biomaterial surface will undoubtedly improve our understanding of the surface and facilitate the amelioration of in vivo performance...
  52. ncbi request reprint Enhancing the biological activity of immobilized osteopontin using a type-1 collagen affinity coating
    Stephanie M Martin
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    J Biomed Mater Res A 70:10-9. 2004
    ..Adhesion of bovine aortic endothelial cells on OPN immobilized using the affinity coating was also significantly enhanced compared to controls. Investigation into the in vivo relevance of this immobilization method is currently underway...
  53. ncbi request reprint Smooth muscle cells deficient in osteopontin have enhanced susceptibility to calcification in vitro
    Mei Y Speer
    Bioengineering Department, Box 351720 University of Washington, Seattle, WA 98195, USA
    Cardiovasc Res 66:324-33. 2005
    ..Osteopontin (OPN) is abundantly expressed in human calcified arteries and inhibits vascular calcification in vitro and in vivo. How OPN functions in vascular calcification, however, is less clear...
  54. ncbi request reprint Upregulation of osteopontin expression in renal cortex of streptozotocin-induced diabetic rats is mediated by bradykinin
    J W Fischer
    Department of Pathology, University of Washington School of Medicine, Seattle, USA
    Diabetes 47:1512-8. 1998
    ..These data suggest that a strong bradykinin B2-receptor-mediated upregulation of osteopontin occurs during the pathogenesis of experimental diabetic nephropathy in rats...
  55. pmc Immobilization of alkaline phosphatase on microporous nanofibrous fibrin scaffolds for bone tissue engineering
    Thanaphum Osathanon
    Department of Oral Biology, School of Dentistry, University of Washington, Seattle, WA 98195, USA
    Biomaterials 30:4513-21. 2009
    ..Furthermore, bone formation in the immobilized ALP/FS treated group was statistically significant compared to FS alone group. However, the response was not robust...
  56. pmc Role of carbonic anhydrase II in ectopic calcification
    Rupak M Rajachar
    Bioengineering Department, University of Washington, Seattle, 98195, USA
    Cardiovasc Pathol 18:77-82. 2009
    ..Previous studies suggested that, in addition to blocking apatite crystal growth, OPN promoted regression of ectopic calcification by inducing the expression of acid-generating carbonic anhydrase II (CAR2) in monocyte-derived cells...
  57. pmc A fibrinogen-based precision microporous scaffold for tissue engineering
    Michael P Linnes
    Department of Bioengineering, University of Washington, 1705 NE Pacific Street, W H Foege Building, Rm N330L, Box 355061, Seattle, WA 98195, USA
    Biomaterials 28:5298-306. 2007
    ..Using these methods we were able to produce microporous fibrin scaffolds that support cell growth and have mechanical properties similar to many native tissues...
  58. doi request reprint Matrix remodeling in vascular calcification associated with chronic kidney disease
    Ashwini S Pai
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    J Am Soc Nephrol 21:1637-40. 2010
    ..Additional studies aimed at further understanding their role are critical for the design of therapeutic interventions...
  59. ncbi request reprint Osteoprotegerin and RANKL differentially regulate angiogenesis and endothelial cell function
    Joseph S McGonigle
    Department of Bioengineering, University of Washington, Seattle, WA, USA
    Angiogenesis 12:35-46. 2009
    ..Our results indicate that OPG is a positive regulator of microvessel formation, while RANKL is an angiogenic inhibitor due to effects on regulation of endothelial cell proliferation, apoptosis, and signaling...
  60. ncbi request reprint Expression and function of the integrin alpha9beta1 in bovine aortic valve interstitial cells
    Lisa M Wiester
    Department of Bioengineering, University of Washington, Seattle, WA 98195 7335, USA
    J Heart Valve Dis 12:605-16. 2003
    ..Since cell-extracellular matrix interactions are critical for tissue morphogenesis, adhesive interactions and integrin function in VIC were investigated...
  61. ncbi request reprint Controlling osteopontin orientation on surfaces to modulate endothelial cell adhesion
    Lingyun Liu
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    J Biomed Mater Res A 74:23-31. 2005
    ..Our studies show that surface properties will alter OPN behavior on surfaces, thus influencing cell interactions...
  62. ncbi request reprint Regulation of cardiovascular calcification
    Mei Y Speer
    Bioengineering Department, University of Washington, Box 351720, Okanogan Lane, Bagley Hall, Seattle, WA 98195, USA
    Cardiovasc Pathol 13:63-70. 2004
    ..Several nonmutually exclusive theories to account for vascular calcification based on current studies are discussed...
  63. ncbi request reprint Adhesive protein interactions with chitosan: consequences for valve endothelial cell growth on tissue-engineering materials
    Janet L Cuy
    Department of Bioengineering, Box 351720, University of Washington, Seattle, Washington 98195, USA
    J Biomed Mater Res A 67:538-47. 2003
    ..These findings suggest potential manipulation of chitosan properties to improve amenability to valve tissue-engineering applications...
  64. ncbi request reprint Esophageal epithelial cell interaction with synthetic and natural scaffolds for tissue engineering
    Benjamin L Beckstead
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Biomaterials 26:6217-28. 2005
    ..Modification of the synthetic scaffold's surface properties and pore size may be necessary to mimic epithelial behavior on natural scaffolds...
  65. ncbi request reprint Mimicking cell-cell interactions at the biomaterial-cell interface for control of stem cell differentiation
    Benjamin L Beckstead
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    J Biomed Mater Res A 79:94-103. 2006
    ..The high potency of bound Jagged-1 suggests that modification of a surface with a Notch ligand presents a powerful method to control stem cell differentiation at the cell-biomaterial interface...
  66. ncbi request reprint Osteoprotegerin inactivation accelerates advanced atherosclerotic lesion progression and calcification in older ApoE-/- mice
    Brian J Bennett
    Department of Pathobiology, University of Washington, Seattle, WA 98195, USA
    Arterioscler Thromb Vasc Biol 26:2117-24. 2006
    ..The current study was designed to assess whether OPG plays a role in the progression and calcification of advanced atherosclerotic lesions in apoE(-/-) mice...
  67. pmc Osteopontin inhibits mineral deposition and promotes regression of ectopic calcification
    Susan A Steitz
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Am J Pathol 161:2035-46. 2002
    ..These findings suggest a novel mechanism of OPN action and potential therapeutic approach to the treatment of ectopic calcification...
  68. pmc Arterial calcification in chronic kidney disease: key roles for calcium and phosphate
    Catherine M Shanahan
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Circ Res 109:697-711. 2011
    ..Unraveling the complex regulatory pathways that mediate the effects of both Ca and P on VSMCs will ultimately provide novel targets and therapies to limit the destructive effects of vascular calcification in CKD patients...
  69. pmc Vitamin D receptor activators induce an anticalcific paracrine program in macrophages: requirement of osteopontin
    Xianwu Li
    University of Washington, Seattle, WA 98195, USA
    Arterioscler Thromb Vasc Biol 30:321-6. 2010
    ..Vascular calcification is highly correlated with morbidity and mortality, and it is often associated with inflammation. Vitamin D may regulate vascular calcification and has been associated with cardiovascular survival benefits...
  70. pmc Characterization of mandibular bone in a mouse model of chronic kidney disease
    Melissa M Lee
    Department of Periodontics, University of Washington School of Dentistry, Seattle, WA 98195 6365, USA
    J Periodontol 81:300-9. 2010
    ..With advances in medical treatment, patients with CKD are living longer and require oral care. The aim of this study is to determine the effects of CKD and dietary phosphate on mandibular bone structure using a uremic mouse model...
  71. ncbi request reprint Effects of cyclosporine in osteopontin null mice
    Marilda Mazzali
    Division of Nephrology, Department of Pathology, University of Washington, Seattle, Washington, USA
    Kidney Int 62:78-85. 2002
    ..This study tested the hypothesis that the absence of OPN would ameliorate CsA nephropathy...
  72. ncbi request reprint Immunohistologic labeling of murine endothelium
    Jitka A Ismail
    Department of Pathology, Box 357470, Room D 514, Health Sciences Building, University of Washington, 1959 Pacific St NE, Seattle, WA 98195, USA
    Cardiovasc Pathol 12:82-90. 2003
    ..With the rising use of mouse models of disease and genetic manipulation, a consistent system to label murine endothelial cells in normal and diseased tissues would be an invaluable tool...
  73. ncbi request reprint Role of the sodium-dependent phosphate cotransporter, Pit-1, in vascular smooth muscle cell calcification
    Xianwu Li
    Department of Bioengineering, University of Washington, Seattle, WA 98195 1720, USA
    Circ Res 98:905-12. 2006
    ..Our results indicate that phosphate uptake through Pit-1 is essential for SMC calcification and phenotypic modulation in response to elevated phosphate...
  74. ncbi request reprint Elevated extracellular calcium levels induce smooth muscle cell matrix mineralization in vitro
    Hsueh Yang
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Kidney Int 66:2293-9. 2004
    ..Hyperphosphatemia, elevated calcium x phosphorus product (Ca x P), and calcium burden, major causes of vascular calcification, are correlated with increased cardiovascular morbidity and mortality in dialysis patients...
  75. ncbi request reprint Calcification of advanced atherosclerotic lesions in the innominate arteries of ApoE-deficient mice: potential role of chondrocyte-like cells
    Marcello Rattazzi
    Department of Pathobiology, University of Washington, Seattle, WA 98195, USA
    Arterioscler Thromb Vasc Biol 25:1420-5. 2005
    ..The time course, cell types, and mechanism(s) associated with calcification were investigated...
  76. pmc Microporous nanofibrous fibrin-based scaffolds for bone tissue engineering
    Thanaphum Osathanon
    Department of Oral Biology, School of Dentistry, University of Washington, Seattle, WA 98195, United States
    Biomaterials 29:4091-9. 2008
    ..25 and 0.5g). All types of scaffolds were degraded both in vitro and in vivo. Furthermore, these scaffolds promoted bone formation in a mouse calvarial defect model and the bone formation was enhanced by addition of rhBMP-2...
  77. ncbi request reprint Biomimetic peptides that engage specific integrin-dependent signaling pathways and bind to calcium phosphate surfaces
    Michele Gilbert
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    J Biomed Mater Res A 67:69-77. 2003
    ..This peptide system can be used to study adhesion-dependent control of signaling in the context of the relevant biomineral surface and may also be useful in biomaterial and tissue engineering applications...
  78. pmc BMP-2 promotes phosphate uptake, phenotypic modulation, and calcification of human vascular smooth muscle cells
    Xianwu Li
    Department of Bioengineering, University of Washington, Seattle, WA 98195 5061, United States
    Atherosclerosis 199:271-7. 2008
    ..Thus, BMP-2 may promote vascular calcification via increased phosphate uptake and induction of osteogenic phenotype modulation in SMC...
  79. ncbi request reprint Delivery of non-viral gene carriers from sphere-templated fibrin scaffolds for sustained transgene expression
    Justin M Saul
    Department of Bioengineering, University of Washington, 1705 NE Pacific Street, W H Foege Building, Room N530P, Mailstop 355061, Seattle, WA 98195, USA
    Biomaterials 28:4705-16. 2007
    ..The integrated material formulations developed in this work provide a useful technology for tissue engineering applications by demonstrating the ability to provide long-term biomimetic cues through non-viral gene delivery...
  80. pmc Methods to promote Notch signaling at the biomaterial interface and evaluation in a rafted organ culture model
    Benjamin L Beckstead
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    J Biomed Mater Res A 91:436-46. 2009
    ..Thus, Notch-signaling biomaterials provide a robust approach to control keratinocyte differentiation and may find application to other progenitor and stem cells...

Research Grants19

  1. Calcification and Vascular Cell Differentiation
    Cecilia Giachelli; Fiscal Year: 2009
    ..These studies will aid in the development of novel therapeutic strategies to prevent and potentially reverse vascular calcification, an urgent need in the ESRD population. ..
  2. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2000
    ..These studies will identify underlying mechanisms regulating ectopic mineralization, and identify novel strategies to prevent native and prosthetic vascular calcification. ..
  3. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2009
    ..Our studies aim to determine mechanisms of vascular calcification such that new targets for therapeutic treatment can be identified. ..
  4. Calcification and Vascular Cell Differentiation
    Cecilia Giachelli; Fiscal Year: 2007
    ..These studies will aid in the development of novel therapeutic strategies to prevent and potentially reverse vascular calcification, an urgent need in the ESRD population. ..
  5. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2007
    ..Specific Aim 3 will determine the mechanism by which osteopontin promotes regression of ectopic calcification in vivo and in vitro. ..
  6. Calcification and Vascular Cell Differentiation
    Cecilia Giachelli; Fiscal Year: 2006
    ..These studies will aid in the development of novel therapeutic strategies to prevent and potentially reverse vascular calcification, an urgent need in the ESRD population. ..
  7. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2006
    ..Specific Aim 3 will determine the mechanism by which osteopontin promotes regression of ectopic calcification in vivo and in vitro. ..
  8. Calcification and Vascular Cell Differentiation
    Cecilia Giachelli; Fiscal Year: 2005
    ..These studies will aid in the development of novel therapeutic strategies to prevent and potentially reverse vascular calcification, an urgent need in the ESRD population. ..
  9. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2005
    ..Specific Aim 3 will determine the mechanism by which osteopontin promotes regression of ectopic calcification in vivo and in vitro. ..
  10. Common Mediators of Vascular Calcification and Bone Dis*
    Cecilia Giachelli; Fiscal Year: 2004
    ..Aim 3 will determine the effect of hyperlipidemia on cartilaginous metaplasia, vascular calcification and osteoporosis in a mouse model of atherosclerosis...
  11. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2004
    ..Specific Aim 3 will determine the mechanism by which osteopontin promotes regression of ectopic calcification in vivo and in vitro. ..
  12. Common Mediators of Vascular Calcification and Bone Dis*
    Cecilia Giachelli; Fiscal Year: 2003
    ..Aim 3 will determine the effect of hyperlipidemia on cartilaginous metaplasia, vascular calcification and osteoporosis in a mouse model of atherosclerosis...
  13. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2002
    ..These studies will identify underlying mechanisms regulating ectopic mineralization, and identify novel strategies to prevent native and prosthetic vascular calcification. ..
  14. Common Mediators of Vascular Calcification and Bone Dis*
    Cecilia Giachelli; Fiscal Year: 2002
    ..Aim 3 will determine the effect of hyperlipidemia on cartilaginous metaplasia, vascular calcification and osteoporosis in a mouse model of atherosclerosis...
  15. Mediators of Vascular Calcification and Bone Disease
    Cecilia Giachelli; Fiscal Year: 2001
    ..Aim 3 will determine the effect of hyperlipidemia on cartilaginous metaplasia, vascular calcification and osteoporosis in a mouse model of atherosclerosis...
  16. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia Giachelli; Fiscal Year: 2001
    ..These studies will identify underlying mechanisms regulating ectopic mineralization, and identify novel strategies to prevent native and prosthetic vascular calcification. ..
  17. MECHANISMS OF VASCULAR CALCIFICATION
    Cecilia M Giachelli; Fiscal Year: 2010
    ..Our studies aim to determine mechanisms of vascular calcification such that new targets for therapeutic treatment can be identified. ..