Partho Ghosh

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Streptococcal M1 protein constructs a pathological host fibrinogen network
    Pauline Macheboeuf
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA
    Nature 472:64-8. 2011
  2. pmc Process of protein transport by the type III secretion system
    Partho Ghosh
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093 0314, USA
    Microbiol Mol Biol Rev 68:771-95. 2004
  3. doi request reprint The nonideal coiled coil of M protein and its multifarious functions in pathogenesis
    Partho Ghosh
    Department of Chemistry and Biochemistry, University of California, San Diego, CA, 92093 0375, USA
    Adv Exp Med Biol 715:197-211. 2011
  4. pmc Coiled-coil irregularities and instabilities in group A Streptococcus M1 are required for virulence
    Case McNamara
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
    Science 319:1405-8. 2008
  5. ncbi request reprint GW domains of the Listeria monocytogenes invasion protein InlB are required for potentiation of Met activation
    Manidipa Banerjee
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0314, USA
    Mol Microbiol 52:257-71. 2004
  6. pmc The type III secretion chaperone SycE promotes a localized disorder-to-order transition in the natively unfolded effector YopE
    Loren Rodgers
    Section of Molecular Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    J Biol Chem 283:20857-63. 2008
  7. ncbi request reprint Characterization of the calcium-binding sites of Listeria monocytogenes InlB
    Michael Marino
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0375, USA
    Biochem Biophys Res Commun 316:379-86. 2004
  8. pmc Selective ligand recognition by a diversity-generating retroelement variable protein
    Jason L Miller
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California, United States of America
    PLoS Biol 6:e131. 2008
  9. pmc Molecular basis for substrate recognition by MTMR2, a myotubularin family phosphoinositide phosphatase
    Michael J Begley
    Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:927-32. 2006
  10. pmc Surface display of a massively variable lipoprotein by a Legionella diversity-generating retroelement
    Diego Arambula
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 110:8212-7. 2013

Collaborators

Detail Information

Publications31

  1. pmc Streptococcal M1 protein constructs a pathological host fibrinogen network
    Pauline Macheboeuf
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA
    Nature 472:64-8. 2011
    ..Disruption of this network into other supramolecular assemblies was not tolerated. These results have bearing on the pathophysiology of streptococcal toxic shock...
  2. pmc Process of protein transport by the type III secretion system
    Partho Ghosh
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093 0314, USA
    Microbiol Mol Biol Rev 68:771-95. 2004
    ..Numerous models explaining how effectors are transported into host cells have been proposed, but understanding of this process is incomplete and this topic remains an active area of inquiry...
  3. doi request reprint The nonideal coiled coil of M protein and its multifarious functions in pathogenesis
    Partho Ghosh
    Department of Chemistry and Biochemistry, University of California, San Diego, CA, 92093 0375, USA
    Adv Exp Med Biol 715:197-211. 2011
    ..In addition, these structural irregularities are reminiscent of those in myosin and tropomyosin, which are targets for crossreactive antibodies in patients suffering from autoimmune sequelae of GAS infection...
  4. pmc Coiled-coil irregularities and instabilities in group A Streptococcus M1 are required for virulence
    Case McNamara
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
    Science 319:1405-8. 2008
    ..Idealized M proteins appear to have promise as vaccine immunogens...
  5. ncbi request reprint GW domains of the Listeria monocytogenes invasion protein InlB are required for potentiation of Met activation
    Manidipa Banerjee
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0314, USA
    Mol Microbiol 52:257-71. 2004
    ..Lastly, we demonstrate that HGF, the endogenous ligand of Met, substitutes for InlB in promoting intracellular invasion, suggesting that no special properties are required of InlB in invasion besides its hormone-like mimicry of HGF...
  6. pmc The type III secretion chaperone SycE promotes a localized disorder-to-order transition in the natively unfolded effector YopE
    Loren Rodgers
    Section of Molecular Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    J Biol Chem 283:20857-63. 2008
    ..The target of this putative signal is unknown but appears to be a bacterial component other than the TTS ATPase YscN...
  7. ncbi request reprint Characterization of the calcium-binding sites of Listeria monocytogenes InlB
    Michael Marino
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0375, USA
    Biochem Biophys Res Commun 316:379-86. 2004
    ..Therefore, our results indicate that the sites are fortuitous in InlB, but also suggest that the simple architecture of the sites may be adaptable for protein engineering purposes...
  8. pmc Selective ligand recognition by a diversity-generating retroelement variable protein
    Jason L Miller
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California, United States of America
    PLoS Biol 6:e131. 2008
    ....
  9. pmc Molecular basis for substrate recognition by MTMR2, a myotubularin family phosphoinositide phosphatase
    Michael J Begley
    Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:927-32. 2006
    ..The conservation of these specificity determinants within the active, but not the inactive, myotubularins provides insight into the functional differences between the active and inactive members...
  10. pmc Surface display of a massively variable lipoprotein by a Legionella diversity-generating retroelement
    Diego Arambula
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 110:8212-7. 2013
    ..Comparative bioinformatics predicts that surface display of massively variable proteins is a defining feature of a subset of bacterial DGRs...
  11. pmc Structure and interactions of the cytoplasmic domain of the Yersinia type III secretion protein YscD
    Alicia Gamez
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA
    J Bacteriol 194:5949-58. 2012
    ..These results suggest that substitutions in the L3 and L4 loops of YscD disrupted the dissociation of SycH from YopH, leading to the accumulation of a large protein complex that stalled the T3S apparatus...
  12. pmc A solvent-exposed patch in chaperone-bound YopE is required for translocation by the type III secretion system
    Loren Rodgers
    Section of Molecular Biology, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
    J Bacteriol 192:3114-22. 2010
    ..These residues form a solvent-exposed patch on the surface of the chaperone-bound Cb region, and thus their effect on translocation is consistent with the structure of the chaperone-bound Cb region serving as a signal for translocation...
  13. pmc Functionally essential interaction between Yersinia YscO and the T3S4 domain of YscP
    Romila Mukerjea
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA
    J Bacteriol 195:4631-8. 2013
    ..These results provide evidence that the interaction of YscO with YscP, and in particular the T3S4 domain of YscP, is essential to type III secretion. ..
  14. pmc Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase
    Maher Alayyoubi
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
    Structure 21:266-76. 2013
    ..This mutational analysis revealed a strict correspondence between retrohoming and interaction of bAvd with bRT, suggesting that the bRT-bAvd complex is important for DGR retrohoming...
  15. pmc M1 protein allows Group A streptococcal survival in phagocyte extracellular traps through cathelicidin inhibition
    Xavier Lauth
    Department of Pediatrics, University of California San Diego, La Jolla, Calif 92093 0687, USA
    J Innate Immun 1:202-14. 2009
    ..We conclude increased resistance to host cathelicidin and killing within phagocyte extracellular traps contribute to the propensity of M1 GAS strains to produce invasive infections...
  16. pmc Conformational flexibility in the multidrug efflux system protein AcrA
    Jonathan Mikolosko
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92037, USA
    Structure 14:577-87. 2006
    ..Notably, unsuspected conformational flexibility in the alpha-helical hairpin domain of AcrA is observed, which has potential mechanistic significance in coupling between AcrA conformations and TolC channel opening...
  17. pmc Specificity and cooperativity at β-lactamase position 104 in TEM-1/BLIP and SHV-1/BLIP interactions
    Melinda S Hanes
    Biophysics Graduate Group, University of California, Berkeley, Berkeley, California 94729, USA
    Proteins 79:1267-76. 2011
    ..We find that BLIP F142 cooperatively stabilizes both interactions, illustrating how a single mutation at a hot spot position can drive large perturbations in interface stability and specificity through a cooperative interaction network...
  18. pmc Global functional analyses of cellular responses to pore-forming toxins
    Cheng Yuan Kao
    Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California, United States of America
    PLoS Pathog 7:e1001314. 2011
    ....
  19. pmc Two translation products of Yersinia yscQ assemble to form a complex essential to type III secretion
    Krzysztof P Bzymek
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093 0375, United States
    Biochemistry 51:1669-77. 2012
    ..A "snap-back" mechanism suggested by the structure can account for this. The 1:2 YscQ-YscQ-C complex is a close mimic of the 1:4 FliM-FliN complex and the likely building block of the putative Yersinia T3S system C-ring...
  20. ncbi request reprint Multiple regions of internalin B contribute to its ability to turn on the Ras-mitogen-activated protein kinase pathway
    Jeremy Copp
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla 92093 0359, USA
    J Biol Chem 278:7783-9. 2003
    ..These unexpected observations suggest that HGF and InlB use alternative mechanisms to turn on cellular signaling pathways...
  21. ncbi request reprint Chemical conjugation of heterologous proteins on the surface of Cowpea mosaic virus
    Anju Chatterji
    Department of Molecular Biology, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Bioconjug Chem 15:807-13. 2004
    ..Strategies demonstrated here allow virus particle targeting to specific cell types and the use of an icosahedral virus as a platform for structure determination of small proteins at moderate resolution...
  22. ncbi request reprint In vivo phospholipase activity of the Pseudomonas aeruginosa cytotoxin ExoU and protection of mammalian cells with phospholipase A2 inhibitors
    Rebecca M Phillips
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093 0314, USA
    J Biol Chem 278:41326-32. 2003
    ....
  23. pmc Conservation of the C-type lectin fold for massive sequence variation in a Treponema diversity-generating retroelement
    Johanne Le Coq
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 108:14649-53. 2011
    ..These results provide strong evidence for the conservation of the formylglycine-generating enzyme-type CLec fold among DGRs as a means of accommodating massive sequence variation...
  24. ncbi request reprint Three-dimensional secretion signals in chaperone-effector complexes of bacterial pathogens
    Sara C Birtalan
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Cell 9:971-80. 2002
    ....
  25. pmc Structure and glycolipid binding properties of the nematicidal protein Cry5B
    Fan Hui
    Department of Chemistry and Biochemistry, 9500 Gilman Drive, University of California, San Diego, La Jolla, CA 92093 0375, USA
    Biochemistry 51:9911-21. 2012
    ..This suggests that the initial binding of Cry5B protoxin to glycolipids can be stabilized at the nematode cell surface by proteolysis. These results lay the groundwork for the design of effective Cry5B-based anthelmintics...
  26. pmc An MHC-I cytoplasmic domain/HIV-1 Nef fusion protein binds directly to the mu subunit of the AP-1 endosomal coat complex
    Rajendra Kumar Singh
    Department of Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 4:e8364. 2009
    ..We previously proposed that Nef allows the MHC-I CD to bind the mu subunit of AP-1 (micro1) as if it contained a Yxxphimotif...
  27. ncbi request reprint The C-type lectin fold as an evolutionary solution for massive sequence variation
    Stephen A McMahon
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093, USA
    Nat Struct Mol Biol 12:886-92. 2005
    ..Mtd variants are biased toward the receptor pertactin, and there is evidence that the CLec fold is used broadly for sequence variation by related retroelements...
  28. pmc GW domains of the Listeria monocytogenes invasion protein InlB are SH3-like and mediate binding to host ligands
    Michael Marino
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla 92093 0314, USA
    EMBO J 21:5623-34. 2002
    ....
  29. ncbi request reprint Identification of a bacterial type III effector family with G protein mimicry functions
    Neal M Alto
    Department of Pharmacology, Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 124:133-45. 2006
    ..The activities of IpgB2, IpgB1, and Map are dependent on an invariant WxxxE motif found in numerous effectors leading to the speculation that they all function by a similar molecular mechanism...
  30. ncbi request reprint Structural elements required for deamidation of RhoA by cytotoxic necrotizing factor 1
    Lori Buetow
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093 0314, USA
    Biochemistry 42:12784-91. 2003
    ..This modest value is consistent with the confining size of the active site pocket acting to exclude nonspecific targets but also limiting reactivity toward intended targets...
  31. pmc Investigation of the mechanism of binding between internalin B and heparin using surface plasmon resonance
    Sybil C Lang Hrtska
    Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, Iowa 52242, USA
    Biochemistry 46:2697-706. 2007
    ..This mode is consistent with enhancement of interaction of InlB with glycosaminoglycans for activation of Met...

Research Grants24

  1. Protein Translocation by the Type III Secretion System
    Partho Ghosh; Fiscal Year: 2009
    ..Our proposal is aimed at understanding how this injection process occurs, and results from our studies are likely to be valuable in the design of antimicrobial strategies aimed at combating these pathogens. ..
  2. Protein Translocation by the Type III Secretion System
    Partho Ghosh; Fiscal Year: 2006
    ..Our proposal is aimed at understanding how this injection process occurs, and results from our studies are likely to be valuable in the design of antimicrobial strategies aimed at combating these pathogens. ..
  3. Mechanism of Host Cell Invasion by Microbial Pathogens
    Partho Ghosh; Fiscal Year: 2006
    ..In addition, the particular pathogen we are studying, Listeria monocytogenes, affects a protein called Met that is involved in some cancers. Thus, results from our studies may also have an impact on cancer therapies. ..
  4. Variable Proteins of Diversity-generating Retroelements
    Partho Ghosh; Fiscal Year: 2010
    ..Our project is aimed at providing basic knowledge on how these variable bacterial proteins recognize diverse targets, with the expectation that this knowledge will have practical applications. ..
  5. Streptococcus M protein and antibody cross-reactivity in rheumatic fever
    Partho Ghosh; Fiscal Year: 2007
    ..The results from our studies are likely to indicate which features of M1 elicit such antibodies, and this information may be applicable to the design of safe and effective streptococcal vaccines. ..
  6. Protein Translocation by the Type III Secretion System
    Partho Ghosh; Fiscal Year: 2007
    ..Our proposal is aimed at understanding how this injection process occurs, and results from our studies are likely to be valuable in the design of antimicrobial strategies aimed at combating these pathogens. ..
  7. Variable Proteins of Diversity-generating Retroelements
    Partho Ghosh; Fiscal Year: 2007
    ..Our project is aimed at providing basic knowledge on how these variable bacterial proteins recognize diverse targets, with the expectation that this knowledge will have practical applications. ..
  8. Structure of the multidrug efflux protein AcrA
    Partho Ghosh; Fiscal Year: 2006
    ..These short-term goals will enable the design of inhibitors of RND efflux pumps, which have the potential of rendering useful many antibiotics that have become clinically ineffective. ..
  9. Variable Proteins of Diversity-generating Retroelements
    Partho Ghosh; Fiscal Year: 2009
    ..Our project is aimed at providing basic knowledge on how these variable bacterial proteins recognize diverse targets, with the expectation that this knowledge will have practical applications. ..
  10. Mechanism of Host Cell Invasion by Microbial Pathogens
    Partho Ghosh; Fiscal Year: 2009
    ..In addition, the particular pathogen we are studying, Listeria monocytogenes, affects a protein called Met that is involved in some cancers. Thus, results from our studies may also have an impact on cancer therapies. ..
  11. Host activation of the Pseudomonas aeruginosa cytoxin ExoU
    Partho Ghosh; Fiscal Year: 2007
    ..The work described here will have potential impact in treating acute infections caused by the opportunistic bacterial pathogen Pseudomonas aeruginosa, and may also have impact on treatment of other bacterial infections. ..
  12. CRYSTAL STRUCTURES OF BACTERIAL PATHOGEN PROTEINS
    Partho Ghosh; Fiscal Year: 2004
    ..This knowledge will be generally applicable to devising strategies to combat L. monocytogenes and other intracellular pathogens. ..
  13. Protein Translocation by the Type III Secretion System
    Partho Ghosh; Fiscal Year: 2010
    ..Our proposal is aimed at understanding how this injection process occurs, and results from our studies are likely to be valuable in the design of antimicrobial strategies aimed at combating these pathogens. ..
  14. Mechanism of Host Cell Invasion by Microbial Pathogens
    Partho Ghosh; Fiscal Year: 2010
    ..In addition, the particular pathogen we are studying, Listeria monocytogenes, affects a protein called Met that is involved in some cancers. Thus, results from our studies may also have an impact on cancer therapies. ..
  15. CRYSTAL STRUCTURES OF BACTERIAL PATHOGEN PROTEINS
    Partho Ghosh; Fiscal Year: 2001
    ..This knowledge will be generally applicable to devising strategies to combat L. monocytogenes and other intracellular pathogens. ..
  16. CRYSTAL STRUCTURES OF BACTERIAL PATHOGEN PROTEINS
    Partho Ghosh; Fiscal Year: 2002
    ..This knowledge will be generally applicable to devising strategies to combat L. monocytogenes and other intracellular pathogens. ..
  17. Mechanism of Host Cell Invasion by Microbial Pathogens
    Partho Ghosh; Fiscal Year: 2007
    ..In addition, the particular pathogen we are studying, Listeria monocytogenes, affects a protein called Met that is involved in some cancers. Thus, results from our studies may also have an impact on cancer therapies. ..
  18. CRYSTAL STRUCTURES OF BACTERIAL PATHOGEN PROTEINS
    Partho Ghosh; Fiscal Year: 2003
    ..This knowledge will be generally applicable to devising strategies to combat L. monocytogenes and other intracellular pathogens. ..
  19. CRYSTAL STRUCTURES OF BACTERIAL PATHOGEN PROTEINS
    Partho Ghosh; Fiscal Year: 2000
    ..This knowledge will be generally applicable to devising strategies to combat L. monocytogenes and other intracellular pathogens. ..