Research Topics
Genomes and GenesSpecies
| Partho GhoshSummaryAffiliation: University of California Country: USA Publications
Research Grants
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Detail Information
Publications
The nonideal coiled coil of M protein and its multifarious functions in pathogenesisPartho Ghosh
Department of Chemistry and Biochemistry, University of California, San Diego, CA, 92093 0375, USA
Adv Exp Med Biol 715:197-211. 2011..In addition, these structural irregularities are reminiscent of those in myosin and tropomyosin, which are targets for crossreactive antibodies in patients suffering from autoimmune sequelae of GAS infection...
Process of protein transport by the type III secretion systemPartho Ghosh
Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093 0314, USA
Microbiol Mol Biol Rev 68:771-95. 2004..Numerous models explaining how effectors are transported into host cells have been proposed, but understanding of this process is incomplete and this topic remains an active area of inquiry...- Streptococcal M1 protein constructs a pathological host fibrinogen networkPauline Macheboeuf
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA
Nature 472:64-8. 2011..Disruption of this network into other supramolecular assemblies was not tolerated. These results have bearing on the pathophysiology of streptococcal toxic shock... 
GW domains of the Listeria monocytogenes invasion protein InlB are required for potentiation of Met activationManidipa Banerjee
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0314, USA
Mol Microbiol 52:257-71. 2004..Lastly, we demonstrate that HGF, the endogenous ligand of Met, substitutes for InlB in promoting intracellular invasion, suggesting that no special properties are required of InlB in invasion besides its hormone-like mimicry of HGF...- The type III secretion chaperone SycE promotes a localized disorder-to-order transition in the natively unfolded effector YopELoren Rodgers
Section of Molecular Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
J Biol Chem 283:20857-63. 2008..The target of this putative signal is unknown but appears to be a bacterial component other than the TTS ATPase YscN... - Coiled-coil irregularities and instabilities in group A Streptococcus M1 are required for virulenceCase McNamara
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
Science 319:1405-8. 2008..Idealized M proteins appear to have promise as vaccine immunogens... - Selective ligand recognition by a diversity-generating retroelement variable proteinJason L Miller
Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California, United States of America
PLoS Biol 6:e131. 2008.... - Characterization of the calcium-binding sites of Listeria monocytogenes InlBMichael Marino
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0375, USA
Biochem Biophys Res Commun 316:379-86. 2004..Therefore, our results indicate that the sites are fortuitous in InlB, but also suggest that the simple architecture of the sites may be adaptable for protein engineering purposes... - Structure and interactions of the cytoplasmic domain of the Yersinia type III secretion protein YscDAlicia Gamez
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA
J Bacteriol 194:5949-58. 2012..These results suggest that substitutions in the L3 and L4 loops of YscD disrupted the dissociation of SycH from YopH, leading to the accumulation of a large protein complex that stalled the T3S apparatus... - A solvent-exposed patch in chaperone-bound YopE is required for translocation by the type III secretion systemLoren Rodgers
Section of Molecular Biology, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
J Bacteriol 192:3114-22. 2010..These residues form a solvent-exposed patch on the surface of the chaperone-bound Cb region, and thus their effect on translocation is consistent with the structure of the chaperone-bound Cb region serving as a signal for translocation... - M1 protein allows Group A streptococcal survival in phagocyte extracellular traps through cathelicidin inhibitionXavier Lauth
Department of Pediatrics, University of California San Diego, La Jolla, Calif 92093 0687, USA
J Innate Immun 1:202-14. 2009..We conclude increased resistance to host cathelicidin and killing within phagocyte extracellular traps contribute to the propensity of M1 GAS strains to produce invasive infections... - Molecular basis for substrate recognition by MTMR2, a myotubularin family phosphoinositide phosphataseMichael J Begley
Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 103:927-32. 2006..The conservation of these specificity determinants within the active, but not the inactive, myotubularins provides insight into the functional differences between the active and inactive members... - Two translation products of Yersinia yscQ assemble to form a complex essential to type III secretionKrzysztof P Bzymek
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093 0375, United States
Biochemistry 51:1669-77. 2012..A "snap-back" mechanism suggested by the structure can account for this. The 1:2 YscQ-YscQ-C complex is a close mimic of the 1:4 FliM-FliN complex and the likely building block of the putative Yersinia T3S system C-ring... - Specificity and cooperativity at β-lactamase position 104 in TEM-1/BLIP and SHV-1/BLIP interactionsMelinda S Hanes
Biophysics Graduate Group, University of California, Berkeley, Berkeley, California 94729, USA
Proteins 79:1267-76. 2011..We find that BLIP F142 cooperatively stabilizes both interactions, illustrating how a single mutation at a hot spot position can drive large perturbations in interface stability and specificity through a cooperative interaction network... - Conformational flexibility in the multidrug efflux system protein AcrAJonathan Mikolosko
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92037, USA
Structure 14:577-87. 2006..Notably, unsuspected conformational flexibility in the alpha-helical hairpin domain of AcrA is observed, which has potential mechanistic significance in coupling between AcrA conformations and TolC channel opening... - Multiple regions of internalin B contribute to its ability to turn on the Ras-mitogen-activated protein kinase pathwayJeremy Copp
Department of Chemistry and Biochemistry, University of California San Diego, La Jolla 92093-0359, USA
J Biol Chem 278:7783-9. 2003..These unexpected observations suggest that HGF and InlB use alternative mechanisms to turn on cellular signaling pathways... - In vivo phospholipase activity of the Pseudomonas aeruginosa cytotoxin ExoU and protection of mammalian cells with phospholipase A2 inhibitorsRebecca M Phillips
Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093-0314, USA
J Biol Chem 278:41326-32. 2003.... - Conservation of the C-type lectin fold for massive sequence variation in a Treponema diversity-generating retroelementJohanne Le Coq
Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 108:14649-53. 2011..These results provide strong evidence for the conservation of the formylglycine-generating enzyme-type CLec fold among DGRs as a means of accommodating massive sequence variation... - An MHC-I cytoplasmic domain/HIV-1 Nef fusion protein binds directly to the mu subunit of the AP-1 endosomal coat complexRajendra Kumar Singh
Department of Medicine, University of California San Diego, La Jolla, California, United States of America
PLoS ONE 4:e8364. 2009..We previously proposed that Nef allows the MHC-I CD to bind the mu subunit of AP-1 (micro1) as if it contained a Yxxphimotif... - Chemical conjugation of heterologous proteins on the surface of Cowpea mosaic virusAnju Chatterji
Department of Molecular Biology, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA
Bioconjug Chem 15:807-13. 2004..Strategies demonstrated here allow virus particle targeting to specific cell types and the use of an icosahedral virus as a platform for structure determination of small proteins at moderate resolution... - Global functional analyses of cellular responses to pore-forming toxinsCheng Yuan Kao
Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California, United States of America
PLoS Pathog 7:e1001314. 2011.... - GW domains of the Listeria monocytogenes invasion protein InlB are SH3-like and mediate binding to host ligandsMichael Marino
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla 92093 0314, USA
EMBO J 21:5623-34. 2002.... - Three-dimensional secretion signals in chaperone-effector complexes of bacterial pathogensSara C Birtalan
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
Mol Cell 9:971-80. 2002.... - Identification of a bacterial type III effector family with G protein mimicry functionsNeal M Alto
Department of Pharmacology, Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
Cell 124:133-45. 2006..The activities of IpgB2, IpgB1, and Map are dependent on an invariant WxxxE motif found in numerous effectors leading to the speculation that they all function by a similar molecular mechanism... - The C-type lectin fold as an evolutionary solution for massive sequence variationStephen A McMahon
Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093, USA
Nat Struct Mol Biol 12:886-92. 2005..Mtd variants are biased toward the receptor pertactin, and there is evidence that the CLec fold is used broadly for sequence variation by related retroelements... - Investigation of the mechanism of binding between internalin B and heparin using surface plasmon resonanceSybil C Lang Hrtska
Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, Iowa 52242, USA
Biochemistry 46:2697-706. 2007..This mode is consistent with enhancement of interaction of InlB with glycosaminoglycans for activation of Met...
Research Grants
- Variable Proteins of Diversity-generating RetroelementsPartho Ghosh; Fiscal Year: 2009..Our project is aimed at providing basic knowledge on how these variable bacterial proteins recognize diverse targets, with the expectation that this knowledge will have practical applications. ..
- Mechanism of Host Cell Invasion by Microbial PathogensPartho Ghosh; Fiscal Year: 2010..In addition, the particular pathogen we are studying, Listeria monocytogenes, affects a protein called Met that is involved in some cancers. Thus, results from our studies may also have an impact on cancer therapies. ..
- Protein Translocation by the Type III Secretion SystemPartho Ghosh; Fiscal Year: 2010..Our proposal is aimed at understanding how this injection process occurs, and results from our studies are likely to be valuable in the design of antimicrobial strategies aimed at combating these pathogens. ..
- Streptococcus M protein and antibody cross-reactivity in rheumatic feverPartho Ghosh; Fiscal Year: 2007..The results from our studies are likely to indicate which features of M1 elicit such antibodies, and this information may be applicable to the design of safe and effective streptococcal vaccines. ..
- Host activation of the Pseudomonas aeruginosa cytoxin ExoUPartho Ghosh; Fiscal Year: 2007..The work described here will have potential impact in treating acute infections caused by the opportunistic bacterial pathogen Pseudomonas aeruginosa, and may also have impact on treatment of other bacterial infections. ..
- CRYSTAL STRUCTURES OF BACTERIAL PATHOGEN PROTEINSPartho Ghosh; Fiscal Year: 2004..This knowledge will be generally applicable to devising strategies to combat L. monocytogenes and other intracellular pathogens. ..
- Structure of the multidrug efflux protein AcrAPartho Ghosh; Fiscal Year: 2006..These short-term goals will enable the design of inhibitors of RND efflux pumps, which have the potential of rendering useful many antibiotics that have become clinically ineffective. ..
- Mechanism of Host Cell Invasion by Microbial PathogensPartho Ghosh; Fiscal Year: 2007..In addition, the particular pathogen we are studying, Listeria monocytogenes, affects a protein called Met that is involved in some cancers. Thus, results from our studies may also have an impact on cancer therapies. ..
- Variable Proteins of Diversity-generating RetroelementsPartho Ghosh; Fiscal Year: 2007..Our project is aimed at providing basic knowledge on how these variable bacterial proteins recognize diverse targets, with the expectation that this knowledge will have practical applications. ..
- Protein Translocation by the Type III Secretion SystemPartho Ghosh; Fiscal Year: 2007..Our proposal is aimed at understanding how this injection process occurs, and results from our studies are likely to be valuable in the design of antimicrobial strategies aimed at combating these pathogens. ..
- Variable Proteins of Diversity-generating RetroelementsPartho Ghosh; Fiscal Year: 2010..Our project is aimed at providing basic knowledge on how these variable bacterial proteins recognize diverse targets, with the expectation that this knowledge will have practical applications. ..