William T Gerthoffer

Summary

Affiliation: University of Nevada
Country: USA

Publications

  1. pmc Endogenous laminin is required for human airway smooth muscle cell maturation
    Thai Tran
    Department of Physiology, University of Manitoba, Winnipeg, MB, Canada
    Respir Res 7:117. 2006
  2. ncbi request reprint Actin cytoskeletal dynamics in smooth muscle contraction
    William T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557 0270, USA
    Can J Physiol Pharmacol 83:851-6. 2005
  3. ncbi request reprint Signal-transduction pathways that regulate visceral smooth muscle function. III. Coupling of muscarinic receptors to signaling kinases and effector proteins in gastrointestinal smooth muscles
    William T Gerthoffer
    University of Nevada School of Medicine, Department of Pharmacology, Reno, Nevada 89557 0270, USA
    Am J Physiol Gastrointest Liver Physiol 288:G849-53. 2005
  4. ncbi request reprint Secretory functions of smooth muscle: cytokines and growth factors
    William T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557 0270, USA
    Mol Interv 2:447-56. 2002
  5. ncbi request reprint MAPK regulation of gene expression in airway smooth muscle
    William T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557 0270, USA
    Respir Physiol Neurobiol 137:237-50. 2003
  6. ncbi request reprint Mechanisms of vascular smooth muscle cell migration
    William T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Circ Res 100:607-21. 2007
  7. ncbi request reprint Invited review: focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle
    W T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 9946, USA
    J Appl Physiol 91:963-72. 2001
  8. pmc Activation of MAP kinases and phosphorylation of caldesmon in canine colonic smooth muscle
    W T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno 89557 0046, USA
    J Physiol 495:597-609. 1996
  9. ncbi request reprint p38 mitogen-activated protein kinase expression and activation in smooth muscle
    J C Hedges
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 0046, USA
    Am J Physiol 275:C527-34. 1998
  10. ncbi request reprint Inflammatory gene expression by human colonic smooth muscle cells
    Sonemany Salinthone
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 0270, USA
    Am J Physiol Gastrointest Liver Physiol 287:G627-37. 2004

Collaborators

Detail Information

Publications45

  1. pmc Endogenous laminin is required for human airway smooth muscle cell maturation
    Thai Tran
    Department of Physiology, University of Manitoba, Winnipeg, MB, Canada
    Respir Res 7:117. 2006
    ..As both changes in ECM components and accumulation of contractile ASM are features of airway wall remodelling in asthma, we examined the role of the ECM protein, laminin, in the maturation of contractile phenotype in human ASM cells...
  2. ncbi request reprint Actin cytoskeletal dynamics in smooth muscle contraction
    William T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557 0270, USA
    Can J Physiol Pharmacol 83:851-6. 2005
    ..Evidence is presented showing that actin filaments are dynamic and that actin-associated proteins comprising the contractile element and actin attachment sites are necessary for smooth muscle contraction...
  3. ncbi request reprint Signal-transduction pathways that regulate visceral smooth muscle function. III. Coupling of muscarinic receptors to signaling kinases and effector proteins in gastrointestinal smooth muscles
    William T Gerthoffer
    University of Nevada School of Medicine, Department of Pharmacology, Reno, Nevada 89557 0270, USA
    Am J Physiol Gastrointest Liver Physiol 288:G849-53. 2005
    ....
  4. ncbi request reprint Secretory functions of smooth muscle: cytokines and growth factors
    William T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557 0270, USA
    Mol Interv 2:447-56. 2002
  5. ncbi request reprint MAPK regulation of gene expression in airway smooth muscle
    William T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557 0270, USA
    Respir Physiol Neurobiol 137:237-50. 2003
    ..These events thereby contribute to normal airway function and participate in pathological changes in ASM that accompany symptoms of asthma...
  6. ncbi request reprint Mechanisms of vascular smooth muscle cell migration
    William T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Circ Res 100:607-21. 2007
    ..Several therapeutically important drugs act by inhibiting cell cycle progression, which may reduce the population of migrating cells...
  7. ncbi request reprint Invited review: focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle
    W T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 9946, USA
    J Appl Physiol 91:963-72. 2001
    ..These signaling pathways are only part of a large number of yet to be defined pathways that mediate acute adaptive responses of the cytoskeleton in smooth muscle to environmental stimuli...
  8. pmc Activation of MAP kinases and phosphorylation of caldesmon in canine colonic smooth muscle
    W T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno 89557 0046, USA
    J Physiol 495:597-609. 1996
    ..Phosphorylation of caldesmon in vivo may reverse inhibitory influences of caldesmon on cross-bridge cycling...
  9. ncbi request reprint p38 mitogen-activated protein kinase expression and activation in smooth muscle
    J C Hedges
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 0046, USA
    Am J Physiol 275:C527-34. 1998
    ..The results suggest that elements of the "stress-response" pathway may be coupled to transcriptional control as well as to cytoskeletal and possibly contractile protein phosphorylation in mammalian smooth muscle...
  10. ncbi request reprint Inflammatory gene expression by human colonic smooth muscle cells
    Sonemany Salinthone
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 0270, USA
    Am J Physiol Gastrointest Liver Physiol 287:G627-37. 2004
    ..SB-203580 was a consistent, efficacious inhibitor of inflammatory gene expression, suggesting an important role of p38 MAPK in synthetic functions of human colonic smooth muscle...
  11. ncbi request reprint Overexpression of human Hsp27 inhibits serum-induced proliferation in airway smooth muscle myocytes and confers resistance to hydrogen peroxide cytotoxicity
    Sonemany Salinthone
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada, USA
    Am J Physiol Lung Cell Mol Physiol 293:L1194-207. 2007
    ..The results support the notion that ASM cells can be stimulated to undergo proliferation and death and that Hsp27 may regulate these processes, thereby contributing to airway remodeling in asthmatics...
  12. ncbi request reprint Synthesis of immune modulators by smooth muscles
    Cherie A Singer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557 0270, USA
    Bioessays 26:646-55. 2004
    ..Appreciating the role of smooth muscle cells in these diseases may lead to better understanding of the beneficial effects of anti-inflammatory drugs as well as identification of new targets for anti-inflammatory therapy...
  13. pmc MicroRNA expression in human airway smooth muscle cells: role of miR-25 in regulation of airway smooth muscle phenotype
    Andrew R Kuhn
    Department of Pharmacology 318, University of Nevada School of Medicine, Reno, NV 89557 0046, USA
    Am J Respir Cell Mol Biol 42:506-13. 2010
    ..This provides novel evidence that miR-25 targets KLF4 in ASM cells and proposes that miR-25 may be an important mediator of ASM phenotype...
  14. ncbi request reprint p38 MAPK and NF-kappaB mediate COX-2 expression in human airway myocytes
    Cherie A Singer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 0046, USA
    Am J Physiol Lung Cell Mol Physiol 285:L1087-98. 2003
    ..These findings suggest that although p38 MAPK and NF-kappaB signaling regulate steady-state levels of COX-2 expression, p38 MAPK additionally affects stability of COX-2 mRNA in cytokine-stimulated human airway myocytes...
  15. ncbi request reprint PKC-epsilon translocation in enteric neurons and interstitial cells of Cajal in response to muscarinic stimulation
    Xuan Yu Wang
    Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Am J Physiol Gastrointest Liver Physiol 285:G593-601. 2003
    ..In conclusion, PKC-epsilon translocation is linked to muscarinic receptor activation in ICC-DMP and a subpopulation of myenteric neurons. These studies demonstrate that ICC-DMP are functionally innervated by excitatory motoneurons...
  16. ncbi request reprint Coupling of M(2) muscarinic receptors to Src activation in cultured canine colonic smooth muscle cells
    Cherie A Singer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 0046, USA
    Am J Physiol Gastrointest Liver Physiol 282:G61-8. 2002
    ..Together these results indicate that M(2) receptors are coupled to Src tyrosine kinase and subsequent activation of ERK in cultured CSMC...
  17. ncbi request reprint p21-activated kinase 1 participates in tracheal smooth muscle cell migration by signaling to p38 Mapk
    M A Dechert
    Cell and Molecular Biology Program, School of Medicine, University of Nevada, Reno, Nevada 89557-0046, USA
    Am J Physiol Cell Physiol 281:C123-32. 2001
    ..Collectively, these results suggest a role for PAK1 in chemotactic TSMC migration that involves catalytic activity and may require signaling to p38 MAPK among other pathways...
  18. pmc Direct evidence for functional smooth muscle myosin II in the 10S self-inhibited monomeric conformation in airway smooth muscle cells
    Deanna L Milton
    Department of Biochemistry and Molecular Biology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Proc Natl Acad Sci U S A 108:1421-6. 2011
    ..This study provides unique direct evidence for the presence of a significant pool of functional myosin in the 10S conformation in cells...
  19. pmc Migration of airway smooth muscle cells
    William T Gerthoffer
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV, USA
    Proc Am Thorac Soc 5:97-105. 2008
    ..Future studies of airway smooth muscle cell migration may uncover novel targets for drugs aimed at modifying airway remodeling...
  20. ncbi request reprint Loss of the alpha7 integrin promotes extracellular signal-regulated kinase activation and altered vascular remodeling
    Jennifer V Welser
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Circ Res 101:672-81. 2007
    ....
  21. ncbi request reprint Evidence for modulation of smooth muscle force by the p38 MAP kinase/HSP27 pathway
    I A Yamboliev
    Department of Pharmacology, University of Nevada School of Medicine, Reno 89557 0046, USA
    Am J Physiol Heart Circ Physiol 278:H1899-907. 2000
    ..These results suggest that ERK MAP kinases phosphorylate caldesmon in vivo but that activation of this pathway is unnecessary for force development. The generation of maximal force may be modulated by the p38 MAP kinase/HSP27 pathway...
  22. ncbi request reprint Phosphatidylinositol 3-kinases regulate ERK and p38 MAP kinases in canine colonic smooth muscle
    I A Yamboliev
    Department of Pharmacology, University of Nevada School of Medicine, Reno 89557 0046, USA
    Am J Physiol Cell Physiol 279:C352-60. 2000
    ....
  23. ncbi request reprint Modulatory role of ERK MAPK-caldesmon pathway in PDGF-stimulated migration of cultured pulmonary artery SMCs
    I A Yamboliev
    Department of Pharmacology, MS 318, University of Nevada School of Medicine, Reno, NV 89557 0046
    Am J Physiol Cell Physiol 280:C1680-8. 2001
    ..We conclude that while ERK MAPK activity is not required to initiate migration, an ERK MAPK-caldesmon pathway may modulate later events necessary for PDGF-stimulated migration of cultured PASMCs...
  24. ncbi request reprint PI 3-kinases and Src kinases regulate spreading and migration of cultured VSMCs
    I A Yamboliev
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557, USA
    Am J Physiol Cell Physiol 281:C709-18. 2001
    ..In conclusion, both PI 3-kinases and Src tyrosine kinases are components of pathways that mediate spreading and migration of cultured PASMCs on collagen...
  25. doi request reprint Tyrosine kinase inhibitors are potent acute pulmonary vasodilators in rats
    Kohtaro Abe
    Department of Pharmacology, University of South Alabama, Mobile, Alabama, USA
    Am J Respir Cell Mol Biol 45:804-8. 2011
    ..Vascular smooth muscle relaxation mediated via activation of myosin light chain phosphatase (Ca(2+) desensitization) appears to play a role in the imatinib-induced pulmonary vasodilation...
  26. pmc HSP20 phosphorylation and airway smooth muscle relaxation
    Mariam Ba
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV, USA
    Cell Health Cytoskelet 2009:27-42. 2009
    ..HSP20 is phosphorylated in vivo in a cAMP-dependent manner and the phosphorylated form promotes airway smooth muscle relaxation, possibly through depolymerization of F-actin as well as inhibition of myosin binding to actin...
  27. pmc Src mediates cytokine-stimulated gene expression in airway myocytes through ERK MAPK
    Cherie A Singer
    University of Nevada School of Medicine, Department of Pharmacology Reno, NV 89557, USA
    Cell Commun Signal 9:14. 2011
    ....
  28. ncbi request reprint Cloning and sequencing of a cDNA encoding the canine HSP27 protein
    J K Larsen
    Department of Pharmacology, University of Nevada School of Medicine, Reno, USA
    Gene 161:305-6. 1995
    ..Similar to human HSP27, the canine protein contains three Ser residues that are potential MAPKAP kinase II substrates...
  29. ncbi request reprint Caveolae facilitate muscarinic receptor-mediated intracellular Ca2+ mobilization and contraction in airway smooth muscle
    Reinoud Gosens
    Department of Physiology, University of Manitoba, John Buhler Research Centre, Winnipeg, Manitoba, Canada
    Am J Physiol Lung Cell Mol Physiol 293:L1406-18. 2007
    ..These results indicate caveolae and caveolin-1 facilitate [Ca(2+)](i) mobilization leading to ASM contraction induced by submaximal concentrations of ACh...
  30. pmc Airway smooth muscle in bronchial tone, inflammation, and remodeling: basic knowledge to clinical relevance
    Reynold A Panettieri
    Airway Biology and Disease Program, Division of Lung Diseases, National Heart, Lung, and Blood Institute, Two Rockledge Center, Suite 10042, 6701 Rockledge Drive, MSC 7952, Bethesda, MD 20892 7952, USA
    Am J Respir Crit Care Med 177:248-52. 2008
    ....
  31. pmc Laminin-binding integrin alpha7 is required for contractile phenotype expression by human airway myocytes
    Thai Tran
    Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada
    Am J Respir Cell Mol Biol 37:668-80. 2007
    ..These observations provide new insight into mechanisms that likely underpin normal development and remodeling associated with airways disease...
  32. pmc Universal physical responses to stretch in the living cell
    Xavier Trepat
    Program in Molecular and Integrative Physiological Sciences, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Nature 447:592-5. 2007
    ..These laws seem to be both universal and primitive, and thus comprise a striking intersection between the worlds of cell biology and soft matter physics...
  33. ncbi request reprint p42/p44 MAP kinase activation is localized to caveolae-free membrane domains in airway smooth muscle
    Reinoud Gosens
    Department of Physiology, University of Manitoba, Manitoba Institute of Child Health, Winnipeg, Manitoba, Canada
    Am J Physiol Lung Cell Mol Physiol 292:L1163-72. 2007
    ..This reveals that directed trafficking of plasma membrane EGFR is an essential element of signal transduction leading to p42/p44 MAP kinase activation...
  34. ncbi request reprint Role of heat shock protein 27 in cytoskeletal remodeling of the airway smooth muscle cell
    Steven S An
    Physiology Program, Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115
    J Appl Physiol 96:1701-13. 2004
    ..Taken together, these results show that phosphorylated HSP27 favors reduced bead motions that are probably due to stabilization of the actin cytoskeleton...
  35. ncbi request reprint Role of caveolin-1 in p42/p44 MAP kinase activation and proliferation of human airway smooth muscle
    Reinoud Gosens
    Department of Physiology, University of Manitoba, Winnipeg, Canada
    Am J Physiol Lung Cell Mol Physiol 291:L523-34. 2006
    ..As such, caveolae and caveolin-1 coordinate PDGF receptor signaling, leading to myocyte proliferation, and inhibit constitutive activity of p42/p44 MAPK to sustain cell quiescence...
  36. ncbi request reprint Novel role for STAT-5B in the regulation of Hsp27-FGF-2 axis facilitating thrombin-induced vascular smooth muscle cell growth and motility
    Huiqing Cao
    Department of Physiology, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Circ Res 98:913-22. 2006
    ....
  37. ncbi request reprint Phosphoinositide-dependent kinase 1 and p21-activated protein kinase mediate reactive oxygen species-dependent regulation of platelet-derived growth factor-induced smooth muscle cell migration
    David S Weber
    Department of Medicine, Division of Cardiology, Emory University, Atlanta, GA 30322, USA
    Circ Res 94:1219-26. 2004
    ..Such information is critical to understanding the role of ROS in vascular diseases in which migration of VSMCs is an important component...
  38. ncbi request reprint Cooperative regulation of GSK-3 by muscarinic and PDGF receptors is associated with airway myocyte proliferation
    Reinoud Gosens
    Departments of Physiology, University of Manitoba, Winnipeg
    Am J Physiol Lung Cell Mol Physiol 293:L1348-58. 2007
    ..Moreover, muscarinic receptors mediate PKC-dependent GSK-3 inhibition, and this appears to be a primary mechanism underpinning augmentation of PDGF-induced cell growth...
  39. ncbi request reprint On the terminology for describing the length-force relationship and its changes in airway smooth muscle
    Tony R Bai
    James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, University of British Columbia, Vancouver, Canada
    J Appl Physiol 97:2029-34. 2004
    ..We also discuss frequently used terms that do not have commonly accepted definitions that should be used cautiously...
  40. ncbi request reprint p38 MAP kinase-dependent regulation of endothelial cell permeability
    Talaibek Borbiev
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 287:L911-8. 2004
    ..These results suggest the involvement of p38 MAP kinase activities and caldesmon phosphorylation in the MLCK-independent regulation of thrombin-induced endothelial cell permeability...
  41. ncbi request reprint Chronic hypoxia modulates relations among calcium, myosin light chain phosphorylation, and force differently in fetal and adult ovine basilar arteries
    Surya M Nauli
    Renal Division, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts, USA
    J Appl Physiol 99:120-7. 2005
    ..Postnatal maturation reprioritizes the mechanisms regulating hypoxic contractility through changes in the source of activator Ca2+, the pathways governing myosin light chain phosphorylation, and its interaction with actin...
  42. ncbi request reprint Angiotensin II-induced activation of p21-activated kinase 1 requires Ca2+ and protein kinase C{delta} in vascular smooth muscle cells
    Elethia A Woolfolk
    Meharry Medical College, Department of Physiology, 1005 DB Todd Blvd, Nashville, TN 37208, USA
    Am J Physiol Cell Physiol 289:C1286-94. 2005
    ..Finally, dnPAK1 markedly inhibited ANG II-induced protein synthesis in VSMCs. These data provide a novel signaling pathway by which ANG II may contribute to vascular remodeling...
  43. ncbi request reprint Expression of the dystrophin-glycoprotein complex is a marker for human airway smooth muscle phenotype maturation
    Pawan Sharma
    Department of Physiology, Section of Respiratory Disease, Univ of Manitoba, Winnipeg, MB, Canada
    Am J Physiol Lung Cell Mol Physiol 294:L57-68. 2008
    ..This provides a strong rationale for future studies investigating the role of the DGC in ASM smooth muscle physiology in health and disease...
  44. ncbi request reprint IL-1beta inhibits intestinal smooth muscle proliferation in an organ culture system: involvement of COX-2 and iNOS induction in muscularis resident macrophages
    Takashi Ohama
    Dept of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The Univ of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    Am J Physiol Gastrointest Liver Physiol 292:G1315-22. 2007
    ..Based on these findings, IL-1beta acts as an anti-proliferative mediator, which acts indirectly through the production of PGE(2) and NO from resident macrophage within ileal smooth muscle tissue...
  45. pmc Myocardin is sufficient for a smooth muscle-like contractile phenotype
    Xiaochun Long
    Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, 211 Bailey Road, Rochester, New York 14586, USA
    Arterioscler Thromb Vasc Biol 28:1505-10. 2008
    ..Here, we sought to ascertain whether Myocd-mediated gene expression confers a structural and physiological cardiac or SMC phenotype...