Sean C Gay

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Crystal structures of cytochrome P450 2B4 in complex with the inhibitor 1-biphenyl-4-methyl-1H-imidazole: ligand-induced structural response through alpha-helical repositioning
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, California 92093, USA
    Biochemistry 48:4762-71. 2009
  2. pmc Crystal structure of a cytochrome P450 2B6 genetic variant in complex with the inhibitor 4-(4-chlorophenyl)imidazole at 2.0-A resolution
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Dr 0703, La Jolla, CA 92093, USA
    Mol Pharmacol 77:529-38. 2010
  3. pmc Structures of cytochrome P450 2B4 complexed with the antiplatelet drugs ticlopidine and clopidogrel
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093, USA
    Biochemistry 49:8709-20. 2010
  4. pmc Structural features of cytochromes P450 and ligands that affect drug metabolism as revealed by X-ray crystallography and NMR
    Sean C Gay
    Future Med Chem 2:1451-68. 2010
  5. pmc Structural analysis of mammalian cytochrome P450 2B4 covalently bound to the mechanism-based inactivator tert-butylphenylacetylene: insight into partial enzymatic activity
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093, USA
    Biochemistry 50:4903-11. 2011
  6. doi request reprint Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme
    P Ross Wilderman
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA
    FEBS J 279:1607-20. 2012
  7. pmc Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity
    Sean C Gay
    Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA
    Acta Crystallogr D Biol Crystallogr 65:1021-31. 2009

Detail Information

Publications7

  1. pmc Crystal structures of cytochrome P450 2B4 in complex with the inhibitor 1-biphenyl-4-methyl-1H-imidazole: ligand-induced structural response through alpha-helical repositioning
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, California 92093, USA
    Biochemistry 48:4762-71. 2009
    ..Comparisons of ligand-bound CYP2B4 structures reveal trends in plastic region mobility that could allow for predictions of their position in future structures based on ligand shape and size...
  2. pmc Crystal structure of a cytochrome P450 2B6 genetic variant in complex with the inhibitor 4-(4-chlorophenyl)imidazole at 2.0-A resolution
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Dr 0703, La Jolla, CA 92093, USA
    Mol Pharmacol 77:529-38. 2010
    ..However, it is unknown whether 2B6 undergoes structural reorganization to accommodate bulkier molecules, as previously inferred from multiple P450 2B4 crystal structures...
  3. pmc Structures of cytochrome P450 2B4 complexed with the antiplatelet drugs ticlopidine and clopidogrel
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093, USA
    Biochemistry 49:8709-20. 2010
    ..Adjusting both protein conformation and ligand orientation in the active site gives 2B4 the flexibility to bind to the widest range of molecules, while also being energetically favorable...
  4. pmc Structural features of cytochromes P450 and ligands that affect drug metabolism as revealed by X-ray crystallography and NMR
    Sean C Gay
    Future Med Chem 2:1451-68. 2010
    ..The first is the various structural elements that microsomal P450s have at their disposal to assume the different conformations observed in X-ray crystal structures. The second is P450-ligand dynamics analyzed by NMR relaxation studies...
  5. pmc Structural analysis of mammalian cytochrome P450 2B4 covalently bound to the mechanism-based inactivator tert-butylphenylacetylene: insight into partial enzymatic activity
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093, USA
    Biochemistry 50:4903-11. 2011
    ..However, docking experiments using this open form show that tBPA is able to rotate upward to give testosterone and 7-ethoxy-4-trifluoromethylcoumarin access to the heme, which could explain the previously observed partial activity...
  6. doi request reprint Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme
    P Ross Wilderman
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA
    FEBS J 279:1607-20. 2012
    ..The results indicate that small changes in the protein scaffold lead to large differences in solution behavior and enzyme function...
  7. pmc Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity
    Sean C Gay
    Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA
    Acta Crystallogr D Biol Crystallogr 65:1021-31. 2009
    ..Therefore, these results provide the first structural insights into a unique hexameric APS kinase that contains a nonfunctional ATP sulfurylase-like domain of unknown function...