Chandrashekhar R Gandhi

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Cholera toxin-sensitive GTP-binding protein-coupled activation of augmenter of liver regeneration (ALR) receptor and its function in rat kupffer cells
    Chandrashekhar R Gandhi
    VA Pittsburgh Healthcare System, Pittsburgh, Pennsylvania, USA
    J Cell Physiol 222:365-73. 2010
  2. pmc Endotoxin causes up-regulation of endothelin receptors in cultured hepatic stellate cells via nitric oxide-dependent and -independent mechanisms
    C R Gandhi
    Department of Surgery, University of Pittsburgh, E 1540 BST, 200 Lothrop Street, Pittsburgh, Pennsylvania, PA 15213, USA
    Br J Pharmacol 131:319-27. 2000
  3. ncbi request reprint Endothelin stimulates transforming growth factor-beta1 and collagen synthesis in stellate cells from control but not cirrhotic rat liver
    C R Gandhi
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1540 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Eur J Pharmacol 406:311-8. 2000
  4. ncbi request reprint Endotoxin treatment causes an upregulation of the endothelin system in the liver: amelioration of increased portal resistance by endothelin receptor antagonism
    C R Gandhi
    Thomas E Starzl Transplantation Institute University of Pittsburgh and Veterans Administration Medical Center Pittsburgh, Pennsylvania 15213, USA
    J Gastroenterol Hepatol 16:61-9. 2001
  5. ncbi request reprint A23187 causes release of inositol phosphates from cultured rat Kupffer cells
    C R Gandhi
    Departments of Surgery and Pathology, Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1540 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Eur J Pharmacol 415:13-8. 2001
  6. pmc Portacaval shunt causes apoptosis and liver atrophy in rats despite increases in endogenous levels of major hepatic growth factors
    Chandrashekhar R Gandhi
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1540 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    J Hepatol 37:340-8. 2002
  7. ncbi request reprint Normal rat hepatic stellate cells respond to endotoxin in LBP-independent manner to produce inhibitor(s) of DNA synthesis in hepatocytes
    Chinnasamy Thirunavukkarasu
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, Pennsylvania
    J Cell Physiol 204:654-65. 2005
  8. pmc Hepatic stellate cells undermine the allostimulatory function of liver myeloid dendritic cells via STAT3-dependent induction of IDO
    Tina L Sumpter
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Immunol 189:3848-58. 2012
  9. doi request reprint Constitutive release of powerful antioxidant-scavenging activity by hepatic stellate cells: protection of hepatocytes from ischemia/reperfusion injury
    Noor Mohamed Jameel
    Thomas E Starzl Transplantation Institute and Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Liver Transpl 16:1400-9. 2010
  10. ncbi request reprint Mechanisms of endotoxin-induced NO, IL-6, and TNF-alpha production in activated rat hepatic stellate cells: role of p38 MAPK
    Chinnasamy Thirunavukkarasu
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Hepatology 44:389-98. 2006

Collaborators

Detail Information

Publications36

  1. pmc Cholera toxin-sensitive GTP-binding protein-coupled activation of augmenter of liver regeneration (ALR) receptor and its function in rat kupffer cells
    Chandrashekhar R Gandhi
    VA Pittsburgh Healthcare System, Pittsburgh, Pennsylvania, USA
    J Cell Physiol 222:365-73. 2010
    ..These results demonstrate specific G-protein coupled binding of ALR and its function in Kupffer cells and suggest that mediators produced by ALR-stimulated Kupffer cells may elicit physiologically important effects on hepatocytes...
  2. pmc Endotoxin causes up-regulation of endothelin receptors in cultured hepatic stellate cells via nitric oxide-dependent and -independent mechanisms
    C R Gandhi
    Department of Surgery, University of Pittsburgh, E 1540 BST, 200 Lothrop Street, Pittsburgh, Pennsylvania, PA 15213, USA
    Br J Pharmacol 131:319-27. 2000
    ..These results demonstrate that endotoxin up-regulates ET-1 receptors in HSC by NO-dependent and -independent mechanisms. Such effects of endotoxin can be of importance in acute endotoxemia and during chronic injury of the liver...
  3. ncbi request reprint Endothelin stimulates transforming growth factor-beta1 and collagen synthesis in stellate cells from control but not cirrhotic rat liver
    C R Gandhi
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1540 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Eur J Pharmacol 406:311-8. 2000
    ....
  4. ncbi request reprint Endotoxin treatment causes an upregulation of the endothelin system in the liver: amelioration of increased portal resistance by endothelin receptor antagonism
    C R Gandhi
    Thomas E Starzl Transplantation Institute University of Pittsburgh and Veterans Administration Medical Center Pittsburgh, Pennsylvania 15213, USA
    J Gastroenterol Hepatol 16:61-9. 2001
    ..Because endothelin-1 (ET-1) has been implicated in endotoxin-induced liver injury, we investigated the hepatic ET-1 system in endotoxin-treated rats...
  5. ncbi request reprint A23187 causes release of inositol phosphates from cultured rat Kupffer cells
    C R Gandhi
    Departments of Surgery and Pathology, Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1540 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Eur J Pharmacol 415:13-8. 2001
    ..We propose that such effects should be considered while evaluating the Ca2+-dependence of biological processes based on the actions of A23187...
  6. pmc Portacaval shunt causes apoptosis and liver atrophy in rats despite increases in endogenous levels of major hepatic growth factors
    Chandrashekhar R Gandhi
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1540 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    J Hepatol 37:340-8. 2002
    ....
  7. ncbi request reprint Normal rat hepatic stellate cells respond to endotoxin in LBP-independent manner to produce inhibitor(s) of DNA synthesis in hepatocytes
    Chinnasamy Thirunavukkarasu
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, Pennsylvania
    J Cell Physiol 204:654-65. 2005
    ..They also indicate that other factors, in addition to NO, TGF-beta, TNF-alpha, IL-1beta, and IL-6 are involved in the mechanisms of the growth inhibitory effects of qHSCs on hepatocytes...
  8. pmc Hepatic stellate cells undermine the allostimulatory function of liver myeloid dendritic cells via STAT3-dependent induction of IDO
    Tina L Sumpter
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Immunol 189:3848-58. 2012
    ..In conclusion, by promoting IDO expression, HSCs may act as potent regulators of liver mDCs and function to maintain hepatic homeostasis and tolerogenicity...
  9. doi request reprint Constitutive release of powerful antioxidant-scavenging activity by hepatic stellate cells: protection of hepatocytes from ischemia/reperfusion injury
    Noor Mohamed Jameel
    Thomas E Starzl Transplantation Institute and Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Liver Transpl 16:1400-9. 2010
    ..Thus, HSCs protect hepatocytes against oxidative damage through the production of a novel protein, the further purification of which may lead to the isolation of a powerful oxygen radical scavenger with clinical applications...
  10. ncbi request reprint Mechanisms of endotoxin-induced NO, IL-6, and TNF-alpha production in activated rat hepatic stellate cells: role of p38 MAPK
    Chinnasamy Thirunavukkarasu
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Hepatology 44:389-98. 2006
    ..In conclusion, endotoxin-induced synthesis of NO, TNF-alpha, and IL-6 in HSCs is mediated by p38 and NF-kappaB, with involvement of H(2)O(2) in TNF-alpha production...
  11. pmc Augmenter of liver regeneration: an important intracellular survival factor for hepatocytes
    Chinnasamy Thirunavukkarasu
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1542 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    J Hepatol 48:578-88. 2008
    ..We sought to determine the effects of ALR depletion in hepatocytes by antisense oligonucleotide transfection...
  12. pmc Augmenter of liver regeneration (ALR) is a novel biomarker of hepatocellular stress/inflammation: in vitro, in vivo and in silico studies
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    Mol Med 18:1421-9. 2013
    ..ALR may thus serve as a potential diagnostic marker of hepatocellular stress and/or acute inflammatory conditions...
  13. pmc p38-MAPK- and caspase-3-mediated superoxide-induced apoptosis of rat hepatic stellate cells: reversal by retinoic acid
    Noor Mohamed Jameel
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, Pennsylvania
    J Cell Physiol 218:157-66. 2009
    ..These results suggest that the absence of retinoids render aHSCs susceptible to superoxide-induced apoptosis via caspase-3 and p38-MAPK activation...
  14. pmc Gliotoxin causes apoptosis and necrosis of rat Kupffer cells in vitro and in vivo in the absence of oxidative stress: exacerbation by caspase and serine protease inhibition
    Kristin Anselmi
    Thomas E Starzl Transplantation Institute, Department of Surgery, and VA Medical Center, University of Pittsburgh, E 1518 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    J Hepatol 47:103-13. 2007
    ..A potential application of gliotoxin therapy for liver fibrosis was suggested by its apoptotic effect on fibrogenic activated stellate cells. We investigated if gliotoxin exerts similar effects on hepatic macrophage Kupffer cells...
  15. pmc Selective expansion of allogeneic regulatory T cells by hepatic stellate cells: role of endotoxin and implications for allograft tolerance
    Anil Dangi
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Immunol 188:3667-77. 2012
    ..These data implicate endotoxin-stimulated HSCs as important immune regulators in liver transplantation by inducing selective expansion of tolerance-promoting Tregs and reducing inflammation and alloimmunity...
  16. ncbi request reprint Kupffer cells are a major source of increased platelet activating factor in the CCl4-induced cirrhotic rat liver
    Yongping Yang
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1542 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    J Hepatol 39:200-7. 2003
    ..The aim of this study was to determine if the responsiveness of Kupffer cells to produce PAF upon ET-1 challenge is modified by cirrhosis...
  17. pmc Prevention of cultured rat stellate cell transformation and endothelin-B receptor upregulation by retinoic acid
    Xuedong Chi
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1540 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Br J Pharmacol 139:765-74. 2003
    ..4 These results showing prevention of HSC activation and negative regulation of ETB receptor expression in them by retinoic acid may have important pathophysiologic implications...
  18. ncbi request reprint Constitutive release of powerful antioxidant-scavenging activity by hepatic stellate cells: Protection of hepatocytes from ischemia/reperfusion injury
    Noor Mohamed Jameel
    Thomas E Starzl Transplantation Institute and Departments of, University of Pittsburgh, Pittsburgh, PA
    Liver Transpl 16:1400-9. 2010
    ..Liver Transpl 16:1400-1409, 2010. © 2010 AASLD...
  19. ncbi request reprint Accelerated reversal of carbon tetrachloride-induced cirrhosis in rats by the endothelin receptor antagonist TAK-044
    Kristin Anselmi
    Department of Surgery, Thomas E Starzl, Transplantation Institute, University of Pittsburgh, PA 15213, USA
    J Gastroenterol Hepatol 17:589-97. 2002
    ..Carbon tetrachloride (CCl4)-induced cirrhosis in rats resolves upon termination of CCl4 treatment. We determined the hepatic ET-1 system during such reversal and assessed whether ET-1 receptor antagonism enhances this process...
  20. pmc miR-122 regulates collagen production via targeting hepatic stellate cells and suppressing P4HA1 expression
    Jiang Li
    Center for Pharmacogenetics, Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, United States
    J Hepatol 58:522-8. 2013
    ..miR-122 has been extensively studied in hepatocarcinogenesis. However, the role of miR-122 in liver fibrosis remains unknown...
  21. pmc Transgenic expression of cyclooxygenase-2 in hepatocytes accelerates endotoxin-induced acute liver failure
    Chang Han
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Immunol 181:8027-35. 2008
    ..Therefore, blocking COX-2-EP(1) pathway may represent a potential approach for amelioration of LPS-induced liver injury...
  22. pmc Inhibition of DNA synthesis in cultured hepatocytes by endotoxin-conditioned medium of activated stellate cells is transforming growth factor-beta and nitric oxide-independent
    T Uemura
    Department of Surgery, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15213
    Br J Pharmacol 133:1125-33. 2001
    ....
  23. pmc A novel mouse model of depletion of stellate cells clarifies their role in ischemia/reperfusion- and endotoxin-induced acute liver injury
    Rachel K Stewart
    Thomas E Starzl Transplantation Institute and Departments of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Hepatol 60:298-305. 2014
    ..However, this function has not been clarified due to unavailability of a model, in which HSCs are depleted from the normal liver...
  24. doi request reprint Endotoxin-stimulated Rat Hepatic Stellate Cells Induce Autophagy in Hepatocytes as a Survival Mechanism
    Anil Dangi
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania
    J Cell Physiol 231:94-105. 2016
    ..While LPS-stimulated HSCs cause apoptosis of a subpopulation of hepatocytes by producing IFNβ, they also induce cell survival mechanisms, which may be of critical importance in resistance to liver injury during endotoxemia...
  25. pmc Liver-specific deletion of augmenter of liver regeneration accelerates development of steatohepatitis and hepatocellular carcinoma in mice
    Chandrashekhar R Gandhi
    Department of Pediatrics, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio Department of Surgery, University of Cincinnati, Cincinnati, Ohio Cincinnati VA Medical Center, Cincinnati, Ohio Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pennsylvania Electronic address
    Gastroenterology 148:379-391.e4. 2015
    ..However, little is known about its roles in hepatic physiology and pathology. We created mice with liver-specific deletion of ALR to study its function...
  26. ncbi request reprint Down-regulation and recovery of endothelin-1 binding and signaling in rat cardiomyocytes
    S A Harvey
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78284 7760, USA
    Biochem Biophys Res Commun 288:558-63. 2001
    ..The recovery of PI-PLC signaling in these cells is in contrast with a previous report, but consistent with recovery of the receptor complement...
  27. pmc Endothelin receptor antagonist TAK-044 arrests and reverses the development of carbon tetrachloride induced cirrhosis in rats
    C Thirunavukkarasu
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Gut 53:1010-9. 2004
    ..We investigated whether ET-1 receptor antagonism, after the development of fibrosis and cirrhosis, arrests/reverses the progression of chronic liver disease...
  28. ncbi request reprint Inhibition of T-cell responses by hepatic stellate cells via B7-H1-mediated T-cell apoptosis in mice
    Ming Chin Yu
    Thomas E Starzl Transplantation Institute and Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Hepatology 40:1312-21. 2004
    ..In conclusion, the bidirectional interactions between HSCs and immune cells may contribute to hepatic immune tolerance...
  29. ncbi request reprint Superoxide-induced apoptosis of activated rat hepatic stellate cells
    Chinnasamy Thirunavukkarasu
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, E 1542 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    J Hepatol 41:567-75. 2004
    ..However, apoptotic aHSCs are also observed during active fibrogenesis in the injured liver. Therefore, we investigated the mechanisms of apoptosis of aHSCs in relation to ROS...
  30. pmc Hepatic stellate cells increase the immunosuppressive function of natural Foxp3+ regulatory T cells via IDO-induced AhR activation
    Sudhir Kumar
    Department of Surgery, University of Cincinnati, Cincinnati, Ohio, USA Cincinnati VA Medical Center, Cincinnati, Ohio, USA
    J Leukoc Biol . 2016
    ..nTregs expanded by cryopreserved HSCs may have potential for clinical use...
  31. pmc The transcriptomic response of rat hepatic stellate cells to endotoxin: implications for hepatic inflammation and immune regulation
    Stephen A K Harvey
    Department of Opthalmology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 8:e82159. 2013
    ....
  32. doi request reprint Involvement of cytosolic phospholipase A2 alpha signalling pathway in spontaneous and transforming growth factor-beta-induced activation of rat hepatic stellate cells
    Liena Zhao
    Department of Pathology and Laboratory Medicine, University of Calgary and Calgary Laboratory Services, Calgary, AB, Canada
    Liver Int 31:1565-73. 2011
    ....
  33. pmc Roles of microRNA-29a in the antifibrotic effect of farnesoid X receptor in hepatic stellate cells
    Jiang Li
    Center for Pharmacogenetics, Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Mol Pharmacol 80:191-200. 2011
    ..Our study uncovers a new mechanism by which FXR negatively regulates the expression of ECM in HSCs...
  34. pmc Increased hepatic platelet activating factor (PAF) and PAF receptors in carbon tetrachloride induced liver cirrhosis
    Y Yang
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Gut 53:877-83. 2004
    ..As PAF is implicated in portal hypertension and hyperdynamic circulation associated with liver cirrhosis, we characterised changes in the hepatic PAF system in experimental cirrhosis...
  35. ncbi request reprint Some morphological, growth, and genomic properties of human cells chronically infected with porcine endogenous retrovirus (PERV)
    Ruhul H Kuddus
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh Medical Center, PA 15261, USA
    Genome 46:858-69. 2003
    ..Finally, PERV infection had no effect on transcription of human endogenous retrovirus-K (HERV-K) genes. Together, no significant effect attributable to PERV infection was evident on chronically PERV-infected HEK-293 cells...
  36. pmc A fresh look at augmenter of liver regeneration in rats
    C R Gandhi
    Thomas E Starzl Transplantation Institute, Veterans Administration Medical Center, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Hepatology 29:1435-45. 1999
    ....