David M Gamm
Affiliation: University of Wisconsin
- Human retinal progenitor cells grown as neurospheres demonstrate time-dependent changes in neuronal and glial cell fate potentialDavid M Gamm
Department of Opthalmology and Visual Sciences, University of Wisconsin, 1500 Highland Avenue, Madison, WI 53705 2280, USA
Ann N Y Acad Sci 1049:107-17. 2005..This provides information for optimizing culture systems aimed at the study of human retinal development and the generation of specific retinal cell types for therapeutic use or drug testing...
- Protection of visual functions by human neural progenitors in a rat model of retinal diseaseDavid M Gamm
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin, United States of America
PLoS ONE 2:e338. 2007..Based on these reports, we explored the potential of hNPC transplantation to rescue visual function in an animal model of retinal degeneration, the Royal College of Surgeons rat...
- A novel serum-free method for culturing human prenatal retinal pigment epithelial cellsDavid M Gamm
Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, T607 Waisman Center, Madison, WI 53705, USA
Invest Ophthalmol Vis Sci 49:788-99. 2008..Therefore, this study was conducted to develop a simple, serum-free system to propagate and sustain human RPE in vitro...
- Regulation of prenatal human retinal neurosphere growth and cell fate potential by retinal pigment epithelium and Mash1David M Gamm
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin 53705, USA
Stem Cells 26:3182-93. 2008....
- Functional analysis of serially expanded human iPS cell-derived RPE culturesRuchira Singh
Waisman Center, University of Wisconsin Madison, Madison, Wisconsin
Invest Ophthalmol Vis Sci 54:6767-78. 2013..To determine the effects of serial expansion on the cellular, molecular, and functional properties of human iPS cell (hiPSC)-derived RPE cultures...
- Optic vesicle-like structures derived from human pluripotent stem cells facilitate a customized approach to retinal disease treatmentJason S Meyer
Waisman Center, University of Wisconsin, Madison, Wisconsin, USA
Stem Cells 29:1206-18. 2011..The production of OV-like populations from human pluripotent stem cells should facilitate the study of human retinal development and disease and advance the use of hiPSCs in personalized medicine...
- Blood-derived human iPS cells generate optic vesicle-like structures with the capacity to form retinal laminae and develop synapsesM Joseph Phillips
Waisman Center, University of Wisconsin Madison, Madison, Wisconsin, USA
Invest Ophthalmol Vis Sci 53:2007-19. 2012..We sought to determine if human induced pluripotent stem cells (iPSCs) derived from blood could produce optic vesicle-like structures (OVs) with the capacity to stratify and express markers of intercellular communication...
- Modeling early retinal development with human embryonic and induced pluripotent stem cellsJason S Meyer
Stem Cell Research Program, Waisman Center, Departments of Anatomy and Neurology, University of Wisconsin Madison, 1500 Highland Avenue, Madison, WI 53705, USA
Proc Natl Acad Sci U S A 106:16698-703. 2009..These findings should aid in the investigation of the molecular events governing retinal specification from human pluripotent stem cells...
- Modeling human retinal development with patient-specific induced pluripotent stem cells reveals multiple roles for visual system homeobox 2M Joseph Phillips
Waisman Center, University of Wisconsin Madison, Madison, Wisconsin, USA McPherson Eye Research Institute, University of Wisconsin Madison, Madison, Wisconsin, USA
Stem Cells 32:1480-92. 2014..Our results establish hiPSC-OVs as versatile model systems to study retinal development at stages not previously accessible in humans and support the bona fide nature of hiPSC-OV-derived retinal progeny...
- Induced pluripotent stem cells as custom therapeutics for retinal repair: progress and rationaleLynda S Wright
Waisman Center, University of Wisconsin, Madison, WI, USA McPherson Eye Research Institute, University of Wisconsin, Madison, WI, USA
Exp Eye Res 123:161-72. 2014..The selection of appropriate hiPSC-derived donor cell type(s) for transplantation will be discussed, as will the caveats and prerequisite steps to formulating a clinical Good Manufacturing Practice (cGMP) product for clinical trials...
- iPS cell modeling of Best disease: insights into the pathophysiology of an inherited macular degenerationRuchira Singh
Waisman Center, University of Wisconsin, Madison, WI 53705, USA
Hum Mol Genet 22:593-607. 2013..In a larger context, this report demonstrates the potential to use patient-specific hiPSCs to model and study maculopathies, an important class of blinding disorders in humans...
- Vitamin D analogs, a new treatment for retinoblastoma: The first Ellsworth LectureDaniel M Albert
Department of Ophthalmology and Visual Sciences, School of Medicine, Madison, WI 53792 3284, USA
Ophthalmic Genet 23:137-56. 2002..We identified vitamin D receptors in retinoblastoma and examined the effectiveness and mechanism of action of these analogs...
- Long-term vision rescue by human neural progenitors in a rat model of photoreceptor degenerationShaomei Wang
Casey Eye Institute, Oregon Health and Sciences University, Portland, Oregon 97239, USA
Invest Ophthalmol Vis Sci 49:3201-6. 2008....
- Culture and Transplantation of Human Retinal SpheresDAVID GAMM; Fiscal Year: 2007..Together, these experiments are designed to build on recent advances in the burgeoning field of stem cell biology and apply them to the basic and clinical science of ophthalmology. ..
- Mechanisms of Retinogenesis in Human Stem CellsDavid M Gamm; Fiscal Year: 2010..Such a model will have great value in the study of basic mechanisms of retinal development and the causes of inherited eye diseases, and will complement our efforts to develop cell-based therapies for degenerative retinal disorders. ..