David Galbraith

Summary

Affiliation: University of Arizona
Country: USA

Publications

  1. doi request reprint High-throughput monitoring of plant nuclear DNA contents via flow cytometry
    David W Galbraith
    School of Plant Sciences, University of Arizona, Tucson, AZ, USA
    Methods Mol Biol 918:311-25. 2012
  2. pmc Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts
    Xristo Zarate
    BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA
    Proteome Sci 8:32. 2010
  3. pmc Development and evaluation of a high-throughput, low-cost genotyping platform based on oligonucleotide microarrays in rice
    Jeremy D Edwards
    University of Florida, Gulf Coast Research and Education Center, Wimauma FL, 33598, USA
    Plant Methods 4:13. 2008
  4. pmc The construction and use of bacterial DNA microarrays based on an optimized two-stage PCR strategy
    Bradley L Postier
    Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078 USA
    BMC Genomics 4:23. 2003
  5. pmc Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells
    Roger A Barthelson
    Bio5 Institute for Collaborative Bioresearch and Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA
    BMC Genomics 8:340. 2007
  6. ncbi request reprint DNA microarray analyses in higher plants
    David W Galbraith
    Department of Plant Sciences, BIO5 Institute, University of Arizona, Tucson, Arizona 85721, USA
    OMICS 10:455-73. 2006
  7. ncbi request reprint Global studies of cell type-specific gene expression in plants
    David W Galbraith
    Department of Plant Sciences and BIO5 Institute, University of Arizona, Tucson, Arizona 85721, USA
    Annu Rev Plant Biol 57:451-75. 2006
  8. doi request reprint Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values
    David W Galbraith
    Department of Plant Sciences and BIO5 Institute for Collaborative Bioresearch, University of Arizona, Tucson Arizona 85721 0240, USA
    Cytometry A 75:692-8. 2009
  9. ncbi request reprint Cytometry and plant sciences: a personal retrospective
    David W Galbraith
    Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA
    Cytometry A 58:37-44. 2004
  10. pmc Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei
    Changqing Zhang
    Department of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA
    Plant Physiol 147:30-40. 2008

Research Grants

  1. Functional Genomics of Nuclei
    David Galbraith; Fiscal Year: 2005

Collaborators

Detail Information

Publications35

  1. doi request reprint High-throughput monitoring of plant nuclear DNA contents via flow cytometry
    David W Galbraith
    School of Plant Sciences, University of Arizona, Tucson, AZ, USA
    Methods Mol Biol 918:311-25. 2012
    ..This chapter provides a general discussion of flow cytometric methods for measuring plant genome sizes, and detailed methods for carrying out these analyses...
  2. pmc Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts
    Xristo Zarate
    BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA
    Proteome Sci 8:32. 2010
    ..A cell-free expression system that synthesizes folded eukaryotic proteins in considerable yields would optimize in vitro translation for protein microarray assembly...
  3. pmc Development and evaluation of a high-throughput, low-cost genotyping platform based on oligonucleotide microarrays in rice
    Jeremy D Edwards
    University of Florida, Gulf Coast Research and Education Center, Wimauma FL, 33598, USA
    Plant Methods 4:13. 2008
    ..In contrast to methods employing whole-genome tiling microarrays for genotyping, our method is based on low-cost spotted microarray production, focusing only on known polymorphic features...
  4. pmc The construction and use of bacterial DNA microarrays based on an optimized two-stage PCR strategy
    Bradley L Postier
    Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078 USA
    BMC Genomics 4:23. 2003
    ..g. over-expression of protein). We describe the successful construction and use of DNA microarrays by the two-stage amplification approach and discuss the technical challenges that were met and resolved during the project...
  5. pmc Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells
    Roger A Barthelson
    Bio5 Institute for Collaborative Bioresearch and Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA
    BMC Genomics 8:340. 2007
    ..One technology, based on microarrays, categorizes gene expression in terms of the abundance of RNA transcripts, and typically employs RNA prepared from whole cells, where cytoplasmic RNA predominates...
  6. ncbi request reprint DNA microarray analyses in higher plants
    David W Galbraith
    Department of Plant Sciences, BIO5 Institute, University of Arizona, Tucson, Arizona 85721, USA
    OMICS 10:455-73. 2006
    ..This article describes, in a necessarily selective manner, the types of platforms currently employed in microarray research and provides an overview of recent activities using these platforms in plant biology...
  7. ncbi request reprint Global studies of cell type-specific gene expression in plants
    David W Galbraith
    Department of Plant Sciences and BIO5 Institute, University of Arizona, Tucson, Arizona 85721, USA
    Annu Rev Plant Biol 57:451-75. 2006
    ..The spatial dissection of the transcriptome has also yielded insights into the localized mediators of hormone inputs and promises to provide detail on cell-specific effects of microRNAs...
  8. doi request reprint Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values
    David W Galbraith
    Department of Plant Sciences and BIO5 Institute for Collaborative Bioresearch, University of Arizona, Tucson Arizona 85721 0240, USA
    Cytometry A 75:692-8. 2009
    ....
  9. ncbi request reprint Cytometry and plant sciences: a personal retrospective
    David W Galbraith
    Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA
    Cytometry A 58:37-44. 2004
  10. pmc Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei
    Changqing Zhang
    Department of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA
    Plant Physiol 147:30-40. 2008
    ..We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem...
  11. ncbi request reprint A framework of integrating gene relations from heterogeneous data sources: an experiment on Arabidopsis thaliana
    Jiexun Li
    Department of Management Information Systems, University of Arizona Tucson, AZ 85721, USA
    Bioinformatics 22:2037-43. 2006
    ..Domain expert judgments on the gene functional clusters in the network confirmed the validity of our approach for relation integration and network inference...
  12. ncbi request reprint Temporal progression of gene expression responses to salt shock in maize roots
    Hong Wang
    Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA
    Plant Mol Biol 52:873-91. 2003
    ..Within this framework, 16 signaling molecules, including six protein kinases, two protein phosphatases and eight transcription factors, were regulated with distinct expression patterns by high salinity...
  13. pmc High-throughput, high-sensitivity analysis of gene expression in Arabidopsis
    Richard Martin Kris
    NuvoGen Research LLC, Tucson, AZ 85728, USA
    Plant Physiol 144:1256-66. 2007
    ....
  14. ncbi request reprint Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley
    Z Neslihan Oztur
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Biosciences West, Tucson 85721 0088, USA
    Plant Mol Biol 48:551-73. 2002
    ....
  15. ncbi request reprint Performance analysis of a dual-buffer architecture for digital flow cytometry
    Shiva Murthi
    Department of Electrical and Computer Engineering, University of Arizona, Tucson, AZ 85721, USA
    Cytometry A 66:109-18. 2005
    ..An improved version of the system is discussed in this paper, focusing on dual-buffering to ensure increased pulse capture. A mathematical model of the system is also presented for performance analysis...
  16. ncbi request reprint Integrative flow cytometric and microarray approaches for use in transcriptional profiling
    David W Galbraith
    Department of Plant Sciences, University of Arizona, Tucson, AZ, USA
    Methods Mol Biol 263:259-80. 2004
    ....
  17. ncbi request reprint The rainbow of fluorescent proteins
    David W Galbraith
    University of Arizona, Department of Plant Sciences, Institute for Biomedical Science and Biotechnology, Tucson, Arizona 85721, USA
    Methods Cell Biol 75:153-69. 2004
  18. ncbi request reprint AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis
    Chun Peng Song
    Department of Plant Sciences, University of Arizona, 303 Forbes Building, Tucson, AZ 85721, USA
    Plant Mol Biol 60:241-57. 2006
    ....
  19. pmc The Oryza bacterial artificial chromosome library resource: construction and analysis of 12 deep-coverage large-insert BAC libraries that represent the 10 genome types of the genus Oryza
    Jetty S S Ammiraju
    Arizona Genomics Institute, University of Arizona, Tucson, Arizona 85721 USA
    Genome Res 16:140-7. 2006
    ....
  20. pmc A probable Na+(K+)/H+ exchanger on the chloroplast envelope functions in pH homeostasis and chloroplast development in Arabidopsis thaliana
    Chun Peng Song
    Department of Plant Sciences, University of Arizona, Tucson, 85721, USA
    Proc Natl Acad Sci U S A 101:10211-6. 2004
    ..chx23 mutants displayed a high sensitivity to NaCl. Together, these data indicate that CHX23 is a probable chloroplast Na(+)(K(+))/H(+) exchanger important for pH homeostasis and chloroplast development and function...
  21. pmc Meiotically stable natural epialleles of Sadhu, a novel Arabidopsis retroposon
    Sanjida H Rangwala
    Department of Biology, Washington University in St Louis, St Louis, Missouri, USA
    PLoS Genet 2:e36. 2006
    ..These findings support the view that an important component of natural variation in retroelements is epigenetic...
  22. ncbi request reprint The sfr6 mutant of Arabidopsis is defective in transcriptional activation via CBF/DREB1 and DREB2 and shows sensitivity to osmotic stress
    Joy M Boyce
    Department of Plant Sciences, University of Oxford, South Parks Road, UK
    Plant J 34:395-406. 2003
    ..Tolerance of osmotic stress was also found to be reduced in sfr6, consistent with a role in osmotic stress tolerance for the cold-, ABA- and drought-inducible genes whose expression is affected by the sfr6 mutation...
  23. ncbi request reprint A gene expression map of the Arabidopsis root
    Kenneth Birnbaum
    Department of Biology, New York University, New York, NY 10003, USA
    Science 302:1956-60. 2003
    ..Chromosomal clustering defined some coregulated genes. This expression map correlates groups of genes to specific cell fates and should serve to guide reverse genetics...
  24. pmc Methods for transcriptional profiling in plants. Be fruitful and replicate
    Blake C Meyers
    Department of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711, USA
    Plant Physiol 135:637-52. 2004
  25. pmc Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors
    Paolo Facella
    ENEA, Trisaia Research Center, Rotondella MT, Italy
    PLoS ONE 3:e2798. 2008
    ..There is considerable experimental evidence demonstrating the roles of photoreceptors in providing light input to the clock...
  26. pmc Salt cress. A halophyte and cryophyte Arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles
    Gunsu Inan
    Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, Indiana 47907 2010, USA
    Plant Physiol 135:1718-37. 2004
    ..In addition, the generation of T-DNA-tagged mutant collections of salt cress, already in progress, will open the door to a new era of forward and reverse genetic studies of extremophile plant biology...
  27. pmc Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression
    Maria Eugenia Zanetti
    Department of Botany and Plant Science, Center for Plant Cell Biology, University of California, Riverside, California 92521 0124, USA
    Plant Physiol 138:624-35. 2005
    ..This technology can be extended to evaluation of mRNA populations at the cell- or tissue-specific level by regulation of the tagged RP with distinct promoters...
  28. ncbi request reprint Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter lines
    Kenneth Birnbaum
    Department of Biology, New York University, 100 Washington Square East, New York, New York 10003, USA
    Nat Methods 2:615-9. 2005
  29. pmc Comparison of RNA expression profiles based on maize expressed sequence tag frequency analysis and micro-array hybridization
    John Fernandes
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    Plant Physiol 128:896-910. 2002
    ..Reassembly of ESTs at the criterion of 90% identity suggests how cross hybridization among gene family members can overestimate the overlap in genes expressed in micro-array hybridization experiments...
  30. pmc Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome
    Charlotte Kristensen
    Plant Biochemistry Laboratory, Department of Plant Biology, and Center for Molecular Plant Physiology, Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Proc Natl Acad Sci U S A 102:1779-84. 2005
    ..thaliana plants with marginal inadvertent effects on the transcriptome and metabolome...
  31. doi request reprint Nanobiotechnology: silica breaks through in plants
    David W Galbraith
    Nat Nanotechnol 2:272-3. 2007
  32. doi request reprint Plant systems biology comes of age
    Joshua S Yuan
    UTIA Genomics Hub, University of Tennessee, Knoxville, TN 37996, USA
    Trends Plant Sci 13:165-71. 2008
    ..Applications of systems biology to plant science have been rapid, and have increased our knowledge about circadian rhythms, multigenic traits, stress responses and plant defenses, and have advanced the virtual plant project...
  33. ncbi request reprint The daunting process of MIAME
    David W Galbraith
    Nature 444:31. 2006

Research Grants2

  1. Functional Genomics of Nuclei
    David Galbraith; Fiscal Year: 2005
    ..Moreover, the constructs will be used to develop mouse lines from which investigators can perform the cell specific gene array studies, as well a studies of cell function within sections of living tissue. ..