Affiliation: University of Maryland
Kazansky Y, Lai M, Singh R, Fushman D. Impact of different ionization states of phosphorylated Serine-65 on ubiquitin structure and interactions. Sci Rep. 2018;8:2651 pubmed publisher
..Our results suggest that the pH-dependence of the conformations and binding properties of phosphorylated Ub and polyUb could provide an additional level of modulation in Ub-mediated signaling. ..
Nowicka U, Zhang D, Walker O, Krutauz D, CastaÃ±eda C, Chaturvedi A, et al
. DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin. Structure. 2015;23:542-57 pubmed publisher
..The dual functionality of Ddi1UBL, capable of binding both ubiquitin and proteasome, suggests an intriguing mechanism for Ddi1 as a proteasomal shuttle. ..
CastaÃ±eda C, Dixon E, Walker O, Chaturvedi A, Nakasone M, Curtis J, et al
. Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins. Structure. 2016;24:423-36 pubmed publisher
..Binding studies and mutagenesis confirmed this prediction, further highlighting structural/recognition versatility of polyubiquitins and the potential power of determining function from elucidation of conformational ensembles. ..
Fushman D. Exploring Polyubiquitin as a Flexible Multiple-Ligand Binding Platform. Structure. 2017;25:1-3 pubmed publisher
..In this issue of Structure, Lin et al. (2017) shed light on how linear tri-ubiquitin binds to ABIN2, a molecule that shares ubiquitin-binding properties of NEMO, the key activator of NF-?B. ..
Chojnacki M, Mansour W, Hameed D, Singh R, El Oualid F, Rosenzweig R, et al
. Polyubiquitin-Photoactivatable Crosslinking Reagents for Mapping Ubiquitin Interactome Identify Rpn1 as a Proteasome Ubiquitin-Associating Subunit. Cell Chem Biol. 2017;24:443-457.e6 pubmed publisher
..Our work demonstrates the value of UbPT, and we expect that its future uses will help define and investigate the ubiquitin interactome. ..
Nakasone M, Lewis T, Walker O, Thakur A, Mansour W, Castaneda C, et al
. Structural Basis for the Inhibitory Effects of Ubistatins in the Ubiquitin-Proteasome Pathway. Structure. 2017;25:1839-1855.e11 pubmed publisher
..These findings highlight versatile properties of ubistatins and have implications for their future development and use in targeting ubiquitin-signaling pathways. ..