Carl W Fuller

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. doi request reprint The challenges of sequencing by synthesis
    Carl W Fuller
    GE Healthcare Life Sciences, Piscataway, New Jersey, USA
    Nat Biotechnol 27:1013-23. 2009
  2. ncbi request reprint Terminal phosphate labeled nucleotides: synthesis, applications, and linker effect on incorporation by DNA polymerases
    Shiv Kumar
    GE Healthcare, 800 Centennial Ave, Piscataway, NJ 08855 1327, USA
    Nucleosides Nucleotides Nucleic Acids 24:401-8. 2005
  3. ncbi request reprint Negatively charged, dye labeled-dideoxynucleotides for "direct-load" DNA sequencing
    Satyam Nampalli
    Amersham Biosciences, Piscataway, New Jersey 08855 1327, USA
    Nucleosides Nucleotides Nucleic Acids 22:1471-4. 2003
  4. pmc Efficient incorporation of positively charged 2', 3'-dideoxynucleoside-5'-triphosphates by DNA polymerases and their application in 'direct-load' DNA sequencing
    Patrick J Finn
    Amersham Biosciences, 800 Centennial Avenue, Piscataway, NJ 08855 1327, USA
    Nucleic Acids Res 31:4769-78. 2003
  5. ncbi request reprint Synthesis and energy transfer efficiency of FRET terminators derived from different linkers
    Shiv Kumar
    Amersham Biosciences, Piscataway, New Jersey 08855 1327, USA
    Nucleosides Nucleotides Nucleic Acids 22:1595-8. 2003
  6. ncbi request reprint Terminal phosphate-labeled nucleotides with improved substrate properties for homogeneous nucleic acid assays
    Anup Sood
    GE Healthcare, 800 Centennial Avenue, Piscataway, New Jersey 08855, USA
    J Am Chem Soc 127:2394-5. 2005
  7. ncbi request reprint Novel cyanine dye-labeled dideoxynucleoside triphosphates for DNA sequencing
    R Scott Duthie
    Amersham Biosciences, 800 Centennial Avenue, Piscataway, New Jersey 08855, USA
    Bioconjug Chem 13:699-706. 2002
  8. pmc Synthesis and application of charge-modified dye-labeled dideoxynucleoside-5'-triphosphates to 'direct-load' DNA sequencing
    Patrick J Finn
    Amersham Biosciences, 800 Centennial Avenue, Piscataway, NJ 08855 1327, USA
    Nucleic Acids Res 30:2877-85. 2002
  9. ncbi request reprint TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing
    John R Nelson
    Genomics Reagents Department, Amersham Biosciences, Piscataway, NJ 08855, USA
    Biotechniques . 2002

Collaborators

  • Shiv Kumar
  • Lei Sun
  • Xiaohua Huang
  • Satyam Nampalli
  • John R Nelson
  • Patrick J Finn
  • Parke K Flick
  • Haiguang Xiao
  • J Anthony Mamone
  • Anup Sood
  • Greg Grossmann
  • R Scott Duthie
  • John Macklin
  • Paula D Phillips
  • Gregory Grossmann
  • Bernard F McArdle
  • Matthew G Bull
  • Theresa L Giesler
  • Yuyang Christine Cai
  • C Palaniappan
  • Mahesh Khot
  • Maria Ortiz-Rivera
  • Lou P Hosta
  • Inna Livshin
  • Joseph W Farchaus
  • Scott Hamilton
  • Shanmuuga T Sundaram
  • Peter L Hewitt
  • Sui Bi Samols
  • Inta M Kalve

Detail Information

Publications9

  1. doi request reprint The challenges of sequencing by synthesis
    Carl W Fuller
    GE Healthcare Life Sciences, Piscataway, New Jersey, USA
    Nat Biotechnol 27:1013-23. 2009
    ..By framing these challenges in a manner accessible to a broad community of scientists and engineers, we hope to solicit input from the broader research community on means of accelerating the advancement of genome sequencing technology...
  2. ncbi request reprint Terminal phosphate labeled nucleotides: synthesis, applications, and linker effect on incorporation by DNA polymerases
    Shiv Kumar
    GE Healthcare, 800 Centennial Ave, Piscataway, NJ 08855 1327, USA
    Nucleosides Nucleotides Nucleic Acids 24:401-8. 2005
    ..These nucleotides have been tested as substrates for different DNA and RNA polymerases. We have also explored their utility in DNA sequencing, SNP analysis, nucleic acid amplification, quantitative PCR, and other biochemical assays...
  3. ncbi request reprint Negatively charged, dye labeled-dideoxynucleotides for "direct-load" DNA sequencing
    Satyam Nampalli
    Amersham Biosciences, Piscataway, New Jersey 08855 1327, USA
    Nucleosides Nucleotides Nucleic Acids 22:1471-4. 2003
    ....
  4. pmc Efficient incorporation of positively charged 2', 3'-dideoxynucleoside-5'-triphosphates by DNA polymerases and their application in 'direct-load' DNA sequencing
    Patrick J Finn
    Amersham Biosciences, 800 Centennial Avenue, Piscataway, NJ 08855 1327, USA
    Nucleic Acids Res 31:4769-78. 2003
    ..The charge-modified nucleotides have also been shown to be more efficiently incorporated by a number of DNA polymerases than regular dye-labeled dideoxynucleotide terminators or indeed normal dideoxynucleoside-5'-triphosphates...
  5. ncbi request reprint Synthesis and energy transfer efficiency of FRET terminators derived from different linkers
    Shiv Kumar
    Amersham Biosciences, Piscataway, New Jersey 08855 1327, USA
    Nucleosides Nucleotides Nucleic Acids 22:1595-8. 2003
    ..These terminators were evaluated for their energy transfer efficiency and DNA sequencing potential using thermostable DNA polymerase...
  6. ncbi request reprint Terminal phosphate-labeled nucleotides with improved substrate properties for homogeneous nucleic acid assays
    Anup Sood
    GE Healthcare, 800 Centennial Avenue, Piscataway, New Jersey 08855, USA
    J Am Chem Soc 127:2394-5. 2005
    ..We have explored the utility of these nucleotides in a variety of applications including homogeneous SNP detection methods, DNA sequencing, and quantitation of PCR and RCA...
  7. ncbi request reprint Novel cyanine dye-labeled dideoxynucleoside triphosphates for DNA sequencing
    R Scott Duthie
    Amersham Biosciences, 800 Centennial Avenue, Piscataway, New Jersey 08855, USA
    Bioconjug Chem 13:699-706. 2002
    ..The two sets of cyanine dye-labeled terminators chosen for this cycle sequencing study produced improved band patterns with band uniformity similar to that obtained with dye-primer sequencing methods...
  8. pmc Synthesis and application of charge-modified dye-labeled dideoxynucleoside-5'-triphosphates to 'direct-load' DNA sequencing
    Patrick J Finn
    Amersham Biosciences, 800 Centennial Avenue, Piscataway, NJ 08855 1327, USA
    Nucleic Acids Res 30:2877-85. 2002
    ..We also present evidence that these charge-modified terminators can relieve gel compression artefacts when used with dGTP in sequencing reactions...
  9. ncbi request reprint TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing
    John R Nelson
    Genomics Reagents Department, Amersham Biosciences, Piscataway, NJ 08855, USA
    Biotechniques . 2002
    ..Once completed, the product DNA can be used directly in sequencing reactions. Additionally, the properties of phi29 DNA polymerase and its use in applications such as amplification ofhuman genomic DNA for genotyping studies is discussed...