Research Topics
| Carl W FullerSummaryAffiliation: University of Pennsylvania Country: USA Publications
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Detail Information
Publications
The challenges of sequencing by synthesisCarl W Fuller
GE Healthcare Life Sciences, Piscataway, New Jersey, USA
Nat Biotechnol 27:1013-23. 2009..By framing these challenges in a manner accessible to a broad community of scientists and engineers, we hope to solicit input from the broader research community on means of accelerating the advancement of genome sequencing technology...
Terminal phosphate labeled nucleotides: synthesis, applications, and linker effect on incorporation by DNA polymerasesShiv Kumar
GE Healthcare, 800 Centennial Ave, Piscataway, NJ 08855-1327, USA
Nucleosides Nucleotides Nucleic Acids 24:401-8. 2005..These nucleotides have been tested as substrates for different DNA and RNA polymerases. We have also explored their utility in DNA sequencing, SNP analysis, nucleic acid amplification, quantitative PCR, and other biochemical assays...
Efficient incorporation of positively charged 2', 3'-dideoxynucleoside-5'-triphosphates by DNA polymerases and their application in 'direct-load' DNA sequencingPatrick J Finn
Amersham Biosciences, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA
Nucleic Acids Res 31:4769-78. 2003..The charge-modified nucleotides have also been shown to be more efficiently incorporated by a number of DNA polymerases than regular dye-labeled dideoxynucleotide terminators or indeed normal dideoxynucleoside-5'-triphosphates...- Negatively charged, dye labeled-dideoxynucleotides for "direct-load" DNA sequencingSatyam Nampalli
Amersham Biosciences, Piscataway, New Jersey 08855-1327, USA
Nucleosides Nucleotides Nucleic Acids 22:1471-4. 2003.... - Synthesis and energy transfer efficiency of FRET terminators derived from different linkersShiv Kumar
Amersham Biosciences, Piscataway, New Jersey 08855 1327, USA
Nucleosides Nucleotides Nucleic Acids 22:1595-8. 2003..These terminators were evaluated for their energy transfer efficiency and DNA sequencing potential using thermostable DNA polymerase... - Terminal phosphate-labeled nucleotides with improved substrate properties for homogeneous nucleic acid assaysAnup Sood
GE Healthcare, 800 Centennial Avenue, Piscataway, New Jersey 08855, USA
J Am Chem Soc 127:2394-5. 2005..We have explored the utility of these nucleotides in a variety of applications including homogeneous SNP detection methods, DNA sequencing, and quantitation of PCR and RCA... - Novel cyanine dye-labeled dideoxynucleoside triphosphates for DNA sequencingR Scott Duthie
Amersham Biosciences, 800 Centennial Avenue, Piscataway, New Jersey 08855, USA
Bioconjug Chem 13:699-706. 2002..The two sets of cyanine dye-labeled terminators chosen for this cycle sequencing study produced improved band patterns with band uniformity similar to that obtained with dye-primer sequencing methods... - Synthesis and application of charge-modified dye-labeled dideoxynucleoside-5'-triphosphates to 'direct-load' DNA sequencingPatrick J Finn
Amersham Biosciences, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA
Nucleic Acids Res 30:2877-85. 2002..We also present evidence that these charge-modified terminators can relieve gel compression artefacts when used with dGTP in sequencing reactions... - TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencingJohn R Nelson
Genomics Reagents Department, Amersham Biosciences, Piscataway, NJ 08855, USA
Biotechniques . 2002..Once completed, the product DNA can be used directly in sequencing reactions. Additionally, the properties of phi29 DNA polymerase and its use in applications such as amplification ofhuman genomic DNA for genotyping studies is discussed...