Genomes and Genes
Affiliation: University of Cincinnati
- Centrosome amplification, chromosome instability and cancer developmentKenji Fukasawa
Department of Cell Biology, University of Cincinnati College of Medicine, P O Box 670521 3125 Eden Ave, Cincinnati, OH 45267 0521, USA
Cancer Lett 230:6-19. 2005..In this review, how centrosome amplification destabilizes chromosomes, how loss of certain tumor suppressor proteins leads to centrosome amplification, and the role of centrosome amplification in cancer development will be discussed...
- Introduction. CentrosomeKenji Fukasawa
Department of Cell Biology, University of Cincinnati College of Medicine, PO Box 670521, Cincinnati, Ohio, OH 45267 0521, USA
Oncogene 21:6140-5. 2002
- Interaction between ROCK II and nucleophosmin/B23 in the regulation of centrosome duplicationZhiyong Ma
Department of Cell Biology, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267 0521, USA
Mol Cell Biol 26:9016-34. 2006..All these findings point to ROCK II as the effector of the CDK2/cyclin E-NPM/B23 pathway in the regulation of centrosome duplication...
- Physical and functional interaction between mortalin and Mps1 kinaseMasayuki Kanai
Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
Genes Cells 12:797-810. 2007..Moreover, Mps1-associated acceleration of centrosome duplication depends on the presence of mortalin and super-activation by the Thr62/Ser65 phosphorylated mortalin...
- Thr199 phosphorylation targets nucleophosmin to nuclear speckles and represses pre-mRNA processingPheruza Tarapore
Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0521, USA
FEBS Lett 580:399-409. 2006..These findings indicate the involvement of NPM in the regulation of pre-mRNA processing, and its activity is controlled by CDK2-mediated phosphorylation on Thr(199)...
- A mammalian in vitro centriole duplication system: evidence for involvement of CDK2/cyclin E and nucleophosmin/B23 in centrosome duplicationPheruza Tarapore
Department of Cell Biology, University of Cincinnati College of Medicine, P O Box 670521, Ohio 45267 0521, USA
Cell Cycle 1:75-81. 2002....
- Characterization of centrosomal association of nucleophosmin/B23 linked to Crm1 activityKazuya Shinmura
Department of Cell Biology, University of Cincinnati College of Medicine, P O Box 670521 3125 Eden Avenue, Cincinnati, OH 45267 0521, United States
FEBS Lett 579:6621-34. 2005..We further found that inhibition of Crm1 nuclear export receptor results in both accumulation of cyclin E at centrosomes and efficient dissociation of NPM/B23 from centrosomes...
- Liver-specific pRB loss results in ectopic cell cycle entry and aberrant ploidyChristopher N Mayhew
Department of Cell Biology, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0521, USA
Cancer Res 65:4568-77. 2005..Together, these results show the crucial role played by pRB in maintaining hepatocyte quiescence and ploidy in adult liver in vivo and underscore the critical importance of delineating the consequences of acute pRB loss in adult animals...
- Analysis of centrosome localization of BRCA1 and its activity in suppressing centrosomal aster formationPheruza Tarapore
Department of Environmental Studies, University of Cincinnati, Cincinnati, OH, USA
Cell Cycle 11:2931-46. 2012..In addition, we identified a new domain of BRCA1 critical for γ-tubulin binding, which confers not only its localization to centrosomes, but also its activity to suppress centrosomal aster formation...
- Oncogenic RAS induces accelerated transition through G2/M and promotes defects in the G2 DNA damage and mitotic spindle checkpointsJeffrey A Knauf
Division of Endocrinology and Metabolism, University of Cincinnati College of Medicine, OH 45267, USA
J Biol Chem 281:3800-9. 2006..We propose that oncogenic RAS activation may predispose cells to genomic instability through both MAPK-dependent and independent pathways that affect critical checkpoints in G(2)/M...
- Induction of centrosome amplification and chromosome instability in p53-null cells by transient exposure to subtoxic levels of S-phase-targeting anticancer drugsRichard A Bennett
Department of Cell Biology, University of Cincinnati College of Medicine, PO Box 670521, Cincinnati, OH 45267 0521, USA
Oncogene 23:6823-9. 2004..This in turn promotes generation of tumor cells equipped with further malignant characteristics...
- Loss of p53 and centrosome hyperamplificationPheruza Tarapore
Department of Cell Biology, University of Cincinnati College of Medicine PO Box 670521, Cincinnati, Ohio, OH 45267 0521, USA
Oncogene 21:6234-40. 2002..p53 appears to exert its transactivation-independent control through direct physical binding to the centrosomes...
- Induction of centrosome amplification and chromosome instability in human bladder cancer cells by p53 mutation and cyclin E overexpressionKenji Kawamura
Department of Cell Biology, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267, USA
Cancer Res 64:4800-9. 2004....
- Active RB elicits late G1/S inhibitionSteven P Angus
Department of Cell Biology, University of Cincinnati College of Medicine, Vontz Center for Molecular Studies, Cincinnati, Ohio 45267 0521, USA
Exp Cell Res 276:201-13. 2002..These studies indicate that RB inhibits cell cycle progression by targeting CDK2/cyclin A-dependent events at the G1/S transition to inhibit cell cycle progression...
- Suppression of centrosome amplification after DNA damage depends on p27 accumulationEiji Sugihara
Nagahama Institute of Bio Science and Technology, Shiga, Japan
Cancer Res 66:4020-9. 2006..These results suggest that the accumulation of p27 after DNA damage is required for suppression of centrosome amplification, thereby preventing chromosomal instability...
- Involvement of Crm1 in hepatitis B virus X protein-induced aberrant centriole replication and abnormal mitotic spindlesMarshonna Forgues
Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute NIH, 37 Convent Drive, Bethesda, MD 20892, USA
Mol Cell Biol 23:5282-92. 2003..Based on this evidence, we suggest that Crm1 is actively involved in maintaining centrosome integrity and that HBx disrupts this process by inactivating Crm1 and thus contributes to HBV-mediated carcinogenesis...
- Oncogenes and tumour suppressors take on centrosomesKenji Fukasawa
Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612, USA
Nat Rev Cancer 7:911-24. 2007..How these proteins control centrosome duplication and function, and how their mutational activation and/or inactivation results in numeral and functional centrosome abnormalities, is discussed in this Review...
- Transcriptional control of BubR1 by p53 and suppression of centrosome amplification by BubR1Tatsuo Oikawa
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 1677 1 Yoshida, Yamaguchi 753 8515, Japan
Mol Cell Biol 25:4046-61. 2005..Our studies demonstrate the molecular aspect of genomic convergence in cultured cells, providing critical information for understanding the stepwise progression of tumors...
- Functional proteomic analysis of melanoma progressionKarine Bernard
Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA
Cancer Res 63:6716-25. 2003..Thus, expression of this antigen likely reports a reduced dependence of protein expression on epidermal interactions...
- Inactivation of E2F3 results in centrosome amplificationHarold I Saavedra
Human Cancer Genetics Program, Department of Molecular Virology, Immunology and Medical Genetics, Children s Research Institute, The Ohio State University, Columbus, OH 43210, USA
Cancer Cell 3:333-46. 2003..Our findings implicate the E2F3 transcription factor as an important link that orchestrates DNA and centrosome duplication cycles, ensuring the faithful transmission of genetic material to daughter cells...
- Involvement of poly(ADP-Ribose) polymerase 1 and poly(ADP-Ribosyl)ation in regulation of centrosome functionMasayuki Kanai
Department of Biochemistry and Molecular Oncology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
Mol Cell Biol 23:2451-62. 2003..These results indicate that PARP-1 and PARP-1-mediated poly(ADP-ribosyl)ation of centrosomal proteins are involved in the regulation of centrosome function...
- Cell cycle arrest and apoptosis induced by human Polo-like kinase 3 is mediated through perturbation of microtubule integrityQi Wang
Department of Medicine and Brander Cancer Research Institute, New York Medical College, Hwathorne, 10532, USA
Mol Cell Biol 22:3450-9. 2002....