K Fujiwara

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. pmc Mechanotransduction in an extracted cell model: Fyn drives stretch- and flow-elicited PECAM-1 phosphorylation
    Yi Jen Chiu
    Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, West Henrietta, NY 14586, USA
    J Cell Biol 182:753-63. 2008
  2. ncbi request reprint Is PECAM-1 a mechanoresponsive molecule?
    K Fujiwara
    Center for Cardiovascular Research, Department of Medicine, University of Rochester, NY 14624, USA
    Cell Struct Funct 26:11-7. 2001
  3. pmc Evidence for a role of platelet endothelial cell adhesion molecule-1 in endothelial cell mechanosignal transduction: is it a mechanoresponsive molecule?
    Masaki Osawa
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Osaka 565 8565, Japan
    J Cell Biol 158:773-85. 2002
  4. ncbi request reprint Endothelial cell-cell adhesion and mechanosignal transduction
    Yi Jen Chiu
    Center for Cardiovascular Research, University of Rochester Medical Center, Rochester, New York 14642, USA
    Endothelium 11:59-73. 2004
  5. ncbi request reprint Platelet endothelial cell adhesion molecule-1 and mechanotransduction in vascular endothelial cells
    K Fujiwara
    Cardiovascular Research Institute, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Intern Med 259:373-80. 2006
  6. ncbi request reprint Stress fiber organization regulated by MLCK and Rho-kinase in cultured human fibroblasts
    K Katoh
    Department of Structural Analysis, National Cardiovascular Center Research Institute, 5 Fujishirodai, Suita, Osaka 565 8565, Japan
    Am J Physiol Cell Physiol 280:C1669-79. 2001
  7. ncbi request reprint Platelet endothelial cell adhesion molecule-1 is a major SH-PTP2 binding protein in vascular endothelial cells
    M Masuda
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Suita, Osaka, Japan
    FEBS Lett 408:331-6. 1997
  8. ncbi request reprint GIT1 mediates thrombin signaling in endothelial cells: role in turnover of RhoA-type focal adhesions
    Geerten P van Nieuw Amerongen
    Center for Cardiovascular Research and Department of Medicine, Aab Institute for Biomedical Sciences, University of Rochester, Rochester, NY 14642, USA
    Circ Res 94:1041-9. 2004
  9. pmc On the tryptophan residue of smooth muscle myosin that responds to binding of nucleotide
    H Onishi
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishiro dai, Suita, Osaka 565 8565, Japan
    Proc Natl Acad Sci U S A 97:11203-8. 2000
  10. pmc Functional transitions in myosin: formation of a critical salt-bridge and transmission of effect to the sensitive tryptophan
    H Onishi
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishiro dai, Suita, Osaka 565 8565, Japan
    Proc Natl Acad Sci U S A 95:6653-8. 1998

Collaborators

Detail Information

Publications23

  1. pmc Mechanotransduction in an extracted cell model: Fyn drives stretch- and flow-elicited PECAM-1 phosphorylation
    Yi Jen Chiu
    Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, West Henrietta, NY 14586, USA
    J Cell Biol 182:753-63. 2008
    ..We suggest that PECAM-1 and Fyn are essential components of a PECAM-1-based mechanosensory complex in ECs...
  2. ncbi request reprint Is PECAM-1 a mechanoresponsive molecule?
    K Fujiwara
    Center for Cardiovascular Research, Department of Medicine, University of Rochester, NY 14624, USA
    Cell Struct Funct 26:11-7. 2001
    ..This PE-CAM-1 phosphorylation initiates a signaling cascade leading to ERK activation. Here we review what is known about PECAM-1 tyrosine phosphorylation and suggest a possible role of PECAM-1 in mechanosensing by endothelial cells...
  3. pmc Evidence for a role of platelet endothelial cell adhesion molecule-1 in endothelial cell mechanosignal transduction: is it a mechanoresponsive molecule?
    Masaki Osawa
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Osaka 565 8565, Japan
    J Cell Biol 158:773-85. 2002
    ..Binding of the beads by itself or pulling on the cell surface using poly-l-coated beads did not induce phosphorylation of PECAM-1 and ERK. These results suggest that PECAM-1 is a mechanotransduction molecule...
  4. ncbi request reprint Endothelial cell-cell adhesion and mechanosignal transduction
    Yi Jen Chiu
    Center for Cardiovascular Research, University of Rochester Medical Center, Rochester, New York 14642, USA
    Endothelium 11:59-73. 2004
    ..We will also review the available evidence for PECAM-1 as a mechanotransducing molecule in endothelial cells...
  5. ncbi request reprint Platelet endothelial cell adhesion molecule-1 and mechanotransduction in vascular endothelial cells
    K Fujiwara
    Cardiovascular Research Institute, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Intern Med 259:373-80. 2006
    ..These results appear to indicate that PECAM-1 is a mechanotransduction molecule. To our knowledge, this is the first mammalian molecule that is shown to respond to mechanical force directly exerted to it...
  6. ncbi request reprint Stress fiber organization regulated by MLCK and Rho-kinase in cultured human fibroblasts
    K Katoh
    Department of Structural Analysis, National Cardiovascular Center Research Institute, 5 Fujishirodai, Suita, Osaka 565 8565, Japan
    Am J Physiol Cell Physiol 280:C1669-79. 2001
    ..The central stress fiber system is dependent more on the activity of Rho-kinase than on that of MLCK, while the peripheral stress fiber system depends on MLCK...
  7. ncbi request reprint Platelet endothelial cell adhesion molecule-1 is a major SH-PTP2 binding protein in vascular endothelial cells
    M Masuda
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Suita, Osaka, Japan
    FEBS Lett 408:331-6. 1997
    ..Our results suggest that PECAM-1 is a component of a mechanosensing machinery acting upstream of SH-PTP2...
  8. ncbi request reprint GIT1 mediates thrombin signaling in endothelial cells: role in turnover of RhoA-type focal adhesions
    Geerten P van Nieuw Amerongen
    Center for Cardiovascular Research and Department of Medicine, Aab Institute for Biomedical Sciences, University of Rochester, Rochester, NY 14642, USA
    Circ Res 94:1041-9. 2004
    ....
  9. pmc On the tryptophan residue of smooth muscle myosin that responds to binding of nucleotide
    H Onishi
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishiro dai, Suita, Osaka 565 8565, Japan
    Proc Natl Acad Sci U S A 97:11203-8. 2000
    ..We suggest that the mutational changes at 512 are transmitted along this path to Cys-717, where they induce changes similar to those caused by reacting wild-type HMM with thiol reagent...
  10. pmc Functional transitions in myosin: formation of a critical salt-bridge and transmission of effect to the sensitive tryptophan
    H Onishi
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishiro dai, Suita, Osaka 565 8565, Japan
    Proc Natl Acad Sci U S A 95:6653-8. 1998
    ..Our results also suggest that structural impairments in the 470-247 region interfere with the transmission of these signals to the responsive Trp...
  11. ncbi request reprint Tyrosine phosphorylation of platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) in mechanically stimulated vascular endothelial cells
    M Osawa
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Osaka Japan
    Eur J Cell Biol 72:229-37. 1997
    ..Our study is the first demonstration of inducible tyrosine phosphorylation of PECAM-1 and suggests involvement of PECAM-1 and Src family kinases in the sensing/signal transduction of mechanical stimuli in ECs...
  12. pmc Rho-kinase--mediated contraction of isolated stress fibers
    K Katoh
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Suita, Osaka 565 8565, Japan
    J Cell Biol 153:569-84. 2001
    ..We propose that Ca(2+) is used to generate rapid contraction, whereas Rho-kinase plays a major role in maintaining sustained contraction in cells...
  13. ncbi request reprint Two new modes of smooth muscle myosin regulation by the interaction between the two regulatory light chains, and by the S2 domain
    K Konishi
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishiro dai, Suita, Osaka 565 8565, Japan
    J Biochem 129:365-72. 2001
    ..We also propose that a cooperative interaction between two motor domains is required for a higher level of actin activation...
  14. ncbi request reprint Serum response factor: master regulator of the actin cytoskeleton and contractile apparatus
    Joseph M Miano
    Cardiovascular Research Institute, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Am J Physiol Cell Physiol 292:C70-81. 2007
    ..This review summarizes the recent genomic and genetic analyses of CArG-SRF that support its role as an ancient, master regulator of the actin cytoskeleton and contractile machinery...
  15. pmc ECM remodeling in hypertensive heart disease
    Bradford C Berk
    Cardiovascular Research Institute and Department of Medicine, University of Rochester, Rochester, NY 14642, USA
    J Clin Invest 117:568-75. 2007
    ..Recent research suggests that therapies targeting the expression, synthesis, or activation of the enzymes responsible for ECM homeostasis might represent novel opportunities to modify the natural progression of HHD...
  16. ncbi request reprint LST-2, a human liver-specific organic anion transporter, determines methotrexate sensitivity in gastrointestinal cancers
    T Abe
    Division of Nephrology, Endocrinology, and Vascular Medicine, Department of Medicine, Tohoku University School of Medicine, Sendai, Japan
    Gastroenterology 120:1689-99. 2001
    ..The aim of this study was to isolate an organic anion transporter that is weak in normal cells, but abundantly expressed in cancer cells, to deliver the anticancer drugs to the cells...
  17. ncbi request reprint Neuron-specific expression of cationic amino acid transporter 3 in the adult rat brain
    H Hosokawa
    Department of Structural Analysis, National Cardiovascular Research Institute, 5 7 1 Fujishirodai, Suita, Osaka 565, Japan
    Brain Res 838:158-65. 1999
    ..The discrepancy between the distribution of mRNA and its translation product suggests a regional difference in the translation rate of the CAT3 transcript...
  18. pmc Down-regulation of ERK but not MEK phosphorylation in cultured endothelial cells by repeated changes in cyclic stretch
    Feng Shi
    Cardiovascular Research Institute, University of Rochester, 601 Elmwood Avenue, Box 679, Rochester, NY 14642, USA
    Cardiovasc Res 73:813-22. 2007
    ..Experiments were designed to study how endothelial cells maintained under certain levels of cyclic stretch responded to shifts in stretch frequencies and amplitudes...
  19. pmc Collagen I matrix turnover is regulated by fibronectin polymerization
    Feng Shi
    Aab Cardiovascular Research Institute, Univ of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Am J Physiol Cell Physiol 298:C1265-75. 2010
    ..These data highlight the complexity of ECM remodeling. This multifaceted regulatory process may be important to ensure tight regulation of ECM fibronectin and collagen I levels...
  20. pmc Phosphorylation and localization of protein-zero related (PZR) in cultured endothelial cells
    Ken ichi Kusano
    Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York 14586, USA
    Endothelium 15:127-36. 2008
    ..These results suggest that PZR is a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts...
  21. ncbi request reprint Vascular endothelial cells that express dystroglycan are involved in angiogenesis
    Hiroshi Hosokawa
    Center for Cardiovascular Research, University of Rochester School of Medicine and Dentistry, Rochester NY 14642 8679, USA
    J Cell Sci 115:1487-96. 2002
    ..Our results suggest that the expression of DG in endothelial cells is under a dynamic regulation and may play a role in angiogenesis...
  22. pmc PKCzeta decreases eNOS protein stability via inhibitory phosphorylation of ERK5
    Patrizia Nigro
    Aab Cardiovascular Research Institute and Department of Medicine, University of Rochester Medical Center, 601 Elmwood Ave, Rochester, NY 14642, USA
    Blood 116:1971-9. 2010
    ..Taken together our results show that PKCĪ¶ binds and phosphorylates ERK5, thereby decreasing eNOS protein stability and contributing to early events of atherosclerosis...
  23. ncbi request reprint Mechanical stresses keep endothelial cells healthy: beneficial effects of a physiological level of cyclic stretch on endothelial barrier function
    Keigi Fujiwara
    Am J Physiol Lung Cell Mol Physiol 285:L782-4. 2003