RAYMOND FRIZZELL

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Regulation of the epithelial sodium channel by membrane trafficking
    Michael B Butterworth
    Dept of Cell Biology and Physiology, Univ of Pittsburgh, S375 BST, 3500 Terrace St, Pittsburgh, PA 15261, USA
    Am J Physiol Renal Physiol 296:F10-24. 2009
  2. pmc Physiology of epithelial chloride and fluid secretion
    Raymond A Frizzell
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cold Spring Harb Perspect Med 2:a009563. 2012
  3. ncbi request reprint The role of regulated CFTR trafficking in epithelial secretion
    Carol A Bertrand
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, S362 BST, 3500 Terrace St, Pittsburgh, PA 15261, USA
    Am J Physiol Cell Physiol 285:C1-18. 2003
  4. ncbi request reprint Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator
    Hui Zhang
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 277:28948-58. 2002
  5. ncbi request reprint Clathrin-mediated endocytosis of the epithelial sodium channel. Role of epsin
    Huamin Wang
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 281:14129-35. 2006
  6. pmc Chaperone displacement from mutant cystic fibrosis transmembrane conductance regulator restores its function in human airway epithelia
    Fei Sun
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    FASEB J 22:3255-63. 2008
  7. ncbi request reprint The deubiquitinating enzyme UCH-L3 regulates the apical membrane recycling of the epithelial sodium channel
    Michael B Butterworth
    Department of Cell Biology and Physiology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Biol Chem 282:37885-93. 2007
  8. pmc Regulation of CFTR trafficking by its R domain
    Christopher M Lewarchik
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 283:28401-12. 2008
  9. pmc Rab11b regulates the apical recycling of the cystic fibrosis transmembrane conductance regulator in polarized intestinal epithelial cells
    Mark R Silvis
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, PA 15261, USA
    Mol Biol Cell 20:2337-50. 2009
  10. pmc SLC26A9 is a constitutively active, CFTR-regulated anion conductance in human bronchial epithelia
    Carol A Bertrand
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Gen Physiol 133:421-38. 2009

Research Grants

  1. Chaperone Actions in CFTR Biogenesis
    Raymond A Frizzell; Fiscal Year: 2010
  2. TRAFFIC REGULATORY PROTEINS AND ENAC
    Raymond A Frizzell; Fiscal Year: 2010
  3. TRAFFIC REGULATORY PROTEINS AND ENAC
    RAYMOND FRIZZELL; Fiscal Year: 2009
  4. Co-chaperone Actions in CFTR Biogenesis
    RAYMOND FRIZZELL; Fiscal Year: 2007
  5. TRAFFIC REGULATORY PROTEINS AND ENAC
    RAYMOND FRIZZELL; Fiscal Year: 2007
  6. TRAFFIC REGULATORY PROTEINS AND ENAC
    RAYMOND FRIZZELL; Fiscal Year: 2002
  7. Chaperone Actions in CFTR Biogenesis
    Raymond A Frizzell; Fiscal Year: 2010

Collaborators

Detail Information

Publications28

  1. pmc Regulation of the epithelial sodium channel by membrane trafficking
    Michael B Butterworth
    Dept of Cell Biology and Physiology, Univ of Pittsburgh, S375 BST, 3500 Terrace St, Pittsburgh, PA 15261, USA
    Am J Physiol Renal Physiol 296:F10-24. 2009
    ..While many details of the regulation of ENaC trafficking remain to be elucidated, knowledge of these mechanisms may provide further insights into ENaC activity in normal and disease states...
  2. pmc Physiology of epithelial chloride and fluid secretion
    Raymond A Frizzell
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cold Spring Harb Perspect Med 2:a009563. 2012
    ....
  3. ncbi request reprint The role of regulated CFTR trafficking in epithelial secretion
    Carol A Bertrand
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, S362 BST, 3500 Terrace St, Pittsburgh, PA 15261, USA
    Am J Physiol Cell Physiol 285:C1-18. 2003
    ..These models provide testable hypotheses that may lead to elucidation of CFTR trafficking mechanisms and permit their experimental manipulation in polarized epithelial cells...
  4. ncbi request reprint Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator
    Hui Zhang
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 277:28948-58. 2002
    ....
  5. ncbi request reprint Clathrin-mediated endocytosis of the epithelial sodium channel. Role of epsin
    Huamin Wang
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 281:14129-35. 2006
    ..These results identify epsin as an accessory protein linking ENaC to the clathrin-based endocytic machinery thereby regulating the activity of this ion channel at the cell surface...
  6. pmc Chaperone displacement from mutant cystic fibrosis transmembrane conductance regulator restores its function in human airway epithelia
    Fei Sun
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    FASEB J 22:3255-63. 2008
    ..Thus, PTD-mediated DeltaFRD fragment delivery may provide a therapy for CF...
  7. ncbi request reprint The deubiquitinating enzyme UCH-L3 regulates the apical membrane recycling of the epithelial sodium channel
    Michael B Butterworth
    Department of Cell Biology and Physiology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Biol Chem 282:37885-93. 2007
    ..This constitutes a novel function for UCH in epithelia and in the regulation of ion channels and demonstrates the dynamic regulation of apically located ENaC by recycling, which is facilitated by this DUB...
  8. pmc Regulation of CFTR trafficking by its R domain
    Christopher M Lewarchik
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 283:28401-12. 2008
    ..These observations suggest that the NEG2 region at the C terminus of the R domain allows stabilization of CFTR in a regulated intracellular compartment from which it traffics to the plasma membrane in response to cAMP/PKA stimulation...
  9. pmc Rab11b regulates the apical recycling of the cystic fibrosis transmembrane conductance regulator in polarized intestinal epithelial cells
    Mark R Silvis
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, PA 15261, USA
    Mol Biol Cell 20:2337-50. 2009
    ..This is the first report detailing apical CFTR recycling in a native expression system and to demonstrate that Rab11b regulates apical recycling in polarized epithelial cells...
  10. pmc SLC26A9 is a constitutively active, CFTR-regulated anion conductance in human bronchial epithelia
    Carol A Bertrand
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Gen Physiol 133:421-38. 2009
    ....
  11. pmc Acute ENaC stimulation by cAMP in a kidney cell line is mediated by exocytic insertion from a recycling channel pool
    Michael B Butterworth
    Department of Cell Biology and Physiology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Gen Physiol 125:81-101. 2005
    ..The model supports the concept of ENaC recycling in response to repetitive cAMP stimulation...
  12. ncbi request reprint Cysteine string protein monitors late steps in cystic fibrosis transmembrane conductance regulator biogenesis
    Hui Zhang
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 281:11312-21. 2006
    ....
  13. ncbi request reprint Derlin-1 promotes the efficient degradation of the cystic fibrosis transmembrane conductance regulator (CFTR) and CFTR folding mutants
    Fei Sun
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 281:36856-63. 2006
    ....
  14. pmc AS160 modulates aldosterone-stimulated epithelial sodium channel forward trafficking
    Xiubin Liang
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Mol Biol Cell 21:2024-33. 2010
    ....
  15. ncbi request reprint Syntaxin 1A regulates ENaC channel activity
    Steven B Condliffe
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15217, USA
    J Biol Chem 279:10085-92. 2004
    ..This rapid effect of S1A may modulate Na(+) entry rate during rapid increases in ENaC N...
  16. pmc An obligatory heterodimer of 14-3-3beta and 14-3-3epsilon is required for aldosterone regulation of the epithelial sodium channel
    Xiubin Liang
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 283:27418-25. 2008
    ....
  17. ncbi request reprint 14-3-3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels
    Xiubin Liang
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, 3500 Terrace Street, Pittsburgh, PA 15261, USA
    J Biol Chem 281:16323-32. 2006
    ..Our studies show that aldosterone increases the expression of 14-3-3beta, which interacts with phospho-Nedd4-2 to block its interaction with ENaC, thus enhancing sodium absorption by increasing apical membrane ENaC density...
  18. pmc Combined bicarbonate conductance-impairing variants in CFTR and SPINK1 variants are associated with chronic pancreatitis in patients without cystic fibrosis
    Alexander Schneider
    Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Gastroenterology 140:162-71. 2011
    ..We investigated whether sequence variants in the gene encoding the pancreatic secretory trypsin inhibitor SPINK1 further increase the risk of pancreatitis in these patients...
  19. pmc Cysteine string protein promotes proteasomal degradation of the cystic fibrosis transmembrane conductance regulator (CFTR) by increasing its interaction with the C terminus of Hsp70-interacting protein and promoting CFTR ubiquitylation
    Bela Z Schmidt
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
    J Biol Chem 284:4168-78. 2009
    ..These findings indicate that Csp not only regulates the exit of CFTR from the ER, but that this action is accompanied by Hsc70/Hsp70 and CHIP-mediated CFTR degradation...
  20. ncbi request reprint Transepithelial impedance analysis of chloride secretion
    Ashvani K Singh
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Med 70:129-42. 2002
  21. pmc Small heat-shock proteins select deltaF508-CFTR for endoplasmic reticulum-associated degradation
    Annette Ahner
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Biol Cell 18:806-14. 2007
    ....
  22. ncbi request reprint Identification and membrane localization of electrogenic sodium bicarbonate cotransporters in Calu-3 cells
    James L Kreindler
    Department of Pediatrics, Children s Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, PA 15213, USA
    Biochim Biophys Acta 1762:704-10. 2006
    ..These data suggest that these transporters contribute to regulated bicarbonate secretion across Calu-3 cells and perhaps human airway submucosal glands...
  23. ncbi request reprint Syntaxin 1A regulates ENaC via domain-specific interactions
    Steven B Condliffe
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 278:12796-804. 2003
    ..This study provides support for the concept that S1A inhibits ENaC-mediated Na(+) transport by decreasing cell surface channel number via direct protein-protein interactions at the ENaC C termini...
  24. pmc Prostasin expression is regulated by airway surface liquid volume and is increased in cystic fibrosis
    Mike M Myerburg
    Division of Pulmonary, Allergy, and Critical Care Medicine, Univ of Pittsburgh Medical Center, 628 NW Montefiore Univ Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213, USA
    Am J Physiol Lung Cell Mol Physiol 294:L932-41. 2008
    ....
  25. ncbi request reprint The epithelial sodium channel (ENaC) traffics to apical membrane in lipid rafts in mouse cortical collecting duct cells
    Warren G Hill
    Division of Matrix Biology, Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    J Biol Chem 282:37402-11. 2007
    ..We conclude that ENaC is present in and regulated by lipid rafts. The data are consistent with a model in which rafts mediate the constitutive apical delivery of ENaC...
  26. ncbi request reprint Airway surface liquid volume regulates ENaC by altering the serine protease-protease inhibitor balance: a mechanism for sodium hyperabsorption in cystic fibrosis
    Mike M Myerburg
    Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 281:27942-9. 2006
    ....
  27. ncbi request reprint Finally, mice with CF lung disease
    Raymond A Frizzell
    Nat Med 10:452-4. 2004
  28. ncbi request reprint Regulation of human cystic fibrosis transmembrane conductance regulator (CFTR) by serum- and glucocorticoid-inducible kinase (SGK1)
    J Denry Sato
    Mount Desert Island Biological Laboratory, Salisbury Cove, USA
    Cell Physiol Biochem 20:91-8. 2007
    ..Because SGK increases the plasma membrane expression of other ion channels, the goal of this paper was to test the hypothesis that SGK1 stimulates CFTR Cl currents by increasing the number of CFTR Cl channels in the plasma membrane...

Research Grants18

  1. Chaperone Actions in CFTR Biogenesis
    Raymond A Frizzell; Fiscal Year: 2010
    ..This novel concept, that sHsps catalyze the transfer of SUMO to their substrates, will be evaluated as a general mechanism of sHsp chaperone function. ..
  2. TRAFFIC REGULATORY PROTEINS AND ENAC
    Raymond A Frizzell; Fiscal Year: 2010
    ..This work is expected to reveal new regulators and therapeutic targets for the control of sodium and fluid transport in the kidney. ..
  3. TRAFFIC REGULATORY PROTEINS AND ENAC
    RAYMOND FRIZZELL; Fiscal Year: 2009
    ..These studies will determine how ENaC's interactions with traffic regulatory proteins controls apical membrane channel density under normal conditions and in response to physiological modulators of Na transport. ..
  4. Co-chaperone Actions in CFTR Biogenesis
    RAYMOND FRIZZELL; Fiscal Year: 2007
    ..Understanding the role of direct, high affinity interactions with Csp may identify targets for favorable and selective modulation of CFTR biogenesis. ..
  5. TRAFFIC REGULATORY PROTEINS AND ENAC
    RAYMOND FRIZZELL; Fiscal Year: 2007
    ..These studies will determine how ENaC's interactions with traffic regulatory proteins controls apical membrane channel density under normal conditions and in response to physiological modulators of Na transport. ..
  6. TRAFFIC REGULATORY PROTEINS AND ENAC
    RAYMOND FRIZZELL; Fiscal Year: 2002
    ..This process has important implications for the regulation of body salt and water homeostasis, the control of blood pressure and cardiovascular diseases. ..
  7. Chaperone Actions in CFTR Biogenesis
    Raymond A Frizzell; Fiscal Year: 2010
    ..This novel concept, that sHsps catalyze the transfer of SUMO to their substrates, will be evaluated as a general mechanism of sHsp chaperone function. ..